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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit

Gene

TPS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Synthase catalytic subunit of the trehalose synthase complex that catalyzes the production of trehalose from glucose-6-phosphate and UDP-alpha-D-glucose in a two step process. Can function independently of the complex.1 Publication

Miscellaneous

Present with 10900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Enzyme regulationi

Activated by fructose 6-phosphate. Inorganic phosphate inhibits the synthase activity in the complex, but activates the synthase activity in the free monomeric form.1 Publication

Kineticsi

  1. KM=3.5 mM for D-glucose 6-phosphate1 Publication
  2. KM=0.5 mM for UDP-alpha-D-glucose1 Publication

    GO - Molecular functioni

    • alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: SGD
    • phosphatase activity Source: GO_Central
    • trehalose-phosphatase activity Source: SGD

    GO - Biological processi

    • cellular carbohydrate metabolic process Source: SGD
    • cellular response to desiccation Source: SGD
    • cellular response to heat Source: SGD
    • cellular response to oxidative stress Source: SGD
    • negative regulation of execution phase of apoptosis Source: SGD
    • trehalose biosynthetic process Source: SGD
    • trehalose metabolism in response to stress Source: GO_Central

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    Biological processStress response

    Enzyme and pathway databases

    BioCyciMetaCyc:YBR126C-MONOMER
    YEAST:YBR126C-MONOMER
    BRENDAi2.4.1.15 984

    Protein family/group databases

    CAZyiGT20 Glycosyltransferase Family 20

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit (EC:2.4.1.15)
    Alternative name(s):
    General glucose sensor subunit 1
    Glycogen metabolism control protein GLC6
    Trehalose synthase complex catalytic subunit TPS1
    Trehalose-6-phosphate synthase
    UDP-glucose-glucosephosphate glucosyltransferase
    Gene namesi
    Name:TPS1
    Synonyms:BYP1, CIF1, FDP1, GGS1, GLC6, TSS1
    Ordered Locus Names:YBR126C
    ORF Names:YBR0922
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome II

    Organism-specific databases

    EuPathDBiFungiDB:YBR126C
    SGDiS000000330 TPS1

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi223H → Y in GLC6-1; low glycogen accumulation. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001225021 – 495Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunitAdd BLAST495

    Proteomic databases

    MaxQBiQ00764
    PaxDbiQ00764
    PRIDEiQ00764
    TopDownProteomicsiQ00764

    PTM databases

    CarbonylDBiQ00764
    iPTMnetiQ00764

    Expressioni

    Inductioni

    Either partial repression by glucose or induction by galactose. Induced by heat shock.

    Interactioni

    Subunit structurei

    The trehalose synthase complex is composed of the two catalytic subunits TPS1 and TPS2 and at least one of the two regulatory subunits TPS3 or TSL1.1 Publication

    Binary interactionsi

    Show more details

    Protein-protein interaction databases

    BioGridi32827, 474 interactors
    ComplexPortaliCPX-582 Trehalose-6-phosphate synthase/phosphatase variant 1
    CPX-583 Trehalose-6-phosphate synthase/phosphatase variant 2
    DIPiDIP-744N
    IntActiQ00764, 39 interactors
    MINTiQ00764
    STRINGi4932.YBR126C

    Structurei

    3D structure databases

    ProteinModelPortaliQ00764
    SMRiQ00764
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 20 family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00550000075557
    HOGENOMiHOG000191477
    InParanoidiQ00764
    KOiK00697
    OMAiIEFMPIH
    OrthoDBiEOG092C0LDC

    Family and domain databases

    CDDicd03788 GT1_TPS, 1 hit
    InterProiView protein in InterPro
    IPR001830 Glyco_trans_20
    IPR012766 Trehalose_OtsA
    PfamiView protein in Pfam
    PF00982 Glyco_transf_20, 1 hit
    TIGRFAMsiTIGR02400 trehalose_OtsA, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q00764-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTTDNAKAQL TSSSGGNIIV VSNRLPVTIT KNSSTGQYEY AMSSGGLVTA
    60 70 80 90 100
    LEGLKKTYTF KWFGWPGLEI PDDEKDQVRK DLLEKFNAVP IFLSDEIADL
    110 120 130 140 150
    HYNGFSNSIL WPLFHYHPGE INFDENAWLA YNEANQTFTN EIAKTMNHND
    160 170 180 190 200
    LIWVHDYHLM LVPEMLRVKI HEKQLQNVKV GWFLHTPFPS SEIYRILPVR
    210 220 230 240 250
    QEILKGVLSC DLVGFHTYDY ARHFLSSVQR VLNVNTLPNG VEYQGRFVNV
    260 270 280 290 300
    GAFPIGIDVD KFTDGLKKES VQKRIQQLKE TFKGCKIIVG VDRLDYIKGV
    310 320 330 340 350
    PQKLHAMEVF LNEHPEWRGK VVLVQVAVPS RGDVEEYQYL RSVVNELVGR
    360 370 380 390 400
    INGQFGTVEF VPIHFMHKSI PFEELISLYA VSDVCLVSST RDGMNLVSYE
    410 420 430 440 450
    YIACQEEKKG SLILSEFTGA AQSLNGAIIV NPWNTDDLSD AINEALTLPD
    460 470 480 490
    VKKEVNWEKL YKYISKYTSA FWGENFVHEL YSTSSSSTSS SATKN
    Length:495
    Mass (Da):56,148
    Last modified:July 1, 1993 - v2
    Checksum:i47325A0FA3554E3D
    GO

    Sequence cautioni

    The sequence AAA66891 differs from that shown. Reason: Frameshift at position 32.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti75K → M in CAA48296 (PubMed:1425702).Curated1
    Sequence conflicti101H → L in CAA48296 (PubMed:1425702).Curated1
    Sequence conflicti104G → W in CAA48296 (PubMed:1425702).Curated1
    Sequence conflicti129 – 130LA → FG in CAA43580 (PubMed:1315471).Curated2
    Sequence conflicti217T → S in CAA48296 (PubMed:1425702).Curated1
    Sequence conflicti288I → L in CAA48296 (PubMed:1425702).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J04450 Genomic DNA Translation: AAA66891.1 Frameshift.
    X68214 mRNA Translation: CAA48296.1
    X68496 Genomic DNA Translation: CAA48510.1
    X67499 Genomic DNA Translation: CAA47834.1
    X61275 Genomic DNA Translation: CAA43580.1
    L21999 Genomic DNA Translation: AAA53672.1
    X78993 Genomic DNA Translation: CAA55627.1 Sequence problems.
    X75891 Genomic DNA Translation: CAA53485.1
    AF061037 mRNA Translation: AAC16974.1
    AY598966 Genomic DNA Translation: AAT27376.1
    AY598964 Genomic DNA Translation: AAT27374.1
    Z35995 Genomic DNA Translation: CAA85083.1
    AY693147 Genomic DNA Translation: AAT93166.1
    BK006936 Genomic DNA Translation: DAA07243.1
    PIRiS34979
    RefSeqiNP_009684.1, NM_001178474.1

    Genome annotation databases

    EnsemblFungiiYBR126C; YBR126C; YBR126C
    GeneIDi852423
    KEGGisce:YBR126C

    Similar proteinsi

    Entry informationi

    Entry nameiTPS1_YEAST
    AccessioniPrimary (citable) accession number: Q00764
    Secondary accession number(s): D6VQC3
    , Q01801, Q05168, Q6J5J4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: July 1, 1993
    Last modified: June 20, 2018
    This is version 183 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

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