Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit

Gene

TPS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Synthase catalytic subunit of the trehalose synthase complex that catalyzes the production of trehalose from glucose-6-phosphate and UDP-glucose in a two step process. Can function independently of the complex.1 Publication

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Enzyme regulationi

Activated by fructose 6-phosphate. Inorganic phosphate inhibits the synthase activity in the complex, but activates the synthase activity in the free monomeric form.1 Publication

Kineticsi

  1. KM=3.5 mM for D-glucose 6-phosphate1 Publication
  2. KM=0.5 mM for UDP-glucose1 Publication

    GO - Molecular functioni

    • alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: SGD

    GO - Biological processi

    • cellular carbohydrate metabolic process Source: SGD
    • cellular response to desiccation Source: SGD
    • cellular response to heat Source: SGD
    • cellular response to oxidative stress Source: SGD
    • dephosphorylation Source: GOC
    • trehalose biosynthetic process Source: SGD
    • trehalose metabolism in response to stress Source: GO_Central
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    BioCyciMetaCyc:YBR126C-MONOMER.
    YEAST:YBR126C-MONOMER.
    BRENDAi2.4.1.15. 984.

    Protein family/group databases

    CAZyiGT20. Glycosyltransferase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit (EC:2.4.1.15)
    Alternative name(s):
    General glucose sensor subunit 1
    Glycogen metabolism control protein GLC6
    Trehalose synthase complex catalytic subunit TPS1
    Trehalose-6-phosphate synthase
    UDP-glucose-glucosephosphate glucosyltransferase
    Gene namesi
    Name:TPS1
    Synonyms:BYP1, CIF1, FDP1, GGS1, GLC6, TSS1
    Ordered Locus Names:YBR126C
    ORF Names:YBR0922
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome II

    Organism-specific databases

    EuPathDBiFungiDB:YBR126C.
    SGDiS000000330. TPS1.

    Subcellular locationi

    GO - Cellular componenti

    • alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi223H → Y in GLC6-1; low glycogen accumulation. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001225021 – 495Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunitAdd BLAST495

    Proteomic databases

    MaxQBiQ00764.
    PRIDEiQ00764.
    TopDownProteomicsiQ00764.

    PTM databases

    iPTMnetiQ00764.

    Expressioni

    Inductioni

    Either partial repression by glucose or induction by galactose. Induced by heat shock.

    Interactioni

    Subunit structurei

    The trehalose synthase complex is composed of the two catalytic subunits TPS1 and TPS2 and at least one of the two regulatory subunits TPS3 or TSL1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-19430,EBI-19430
    TPS2P316888EBI-19430,EBI-19440
    TPS3P384266EBI-19430,EBI-19448
    TSL1P384277EBI-19430,EBI-19638

    Protein-protein interaction databases

    BioGridi32827. 226 interactors.
    DIPiDIP-744N.
    IntActiQ00764. 26 interactors.
    MINTiMINT-618278.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00764.
    SMRiQ00764.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 20 family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00550000075557.
    HOGENOMiHOG000191477.
    InParanoidiQ00764.
    KOiK00697.
    OMAiWLAYNEA.
    OrthoDBiEOG092C0LDC.

    Family and domain databases

    CDDicd03788. GT1_TPS. 1 hit.
    InterProiIPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q00764-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTTDNAKAQL TSSSGGNIIV VSNRLPVTIT KNSSTGQYEY AMSSGGLVTA
    60 70 80 90 100
    LEGLKKTYTF KWFGWPGLEI PDDEKDQVRK DLLEKFNAVP IFLSDEIADL
    110 120 130 140 150
    HYNGFSNSIL WPLFHYHPGE INFDENAWLA YNEANQTFTN EIAKTMNHND
    160 170 180 190 200
    LIWVHDYHLM LVPEMLRVKI HEKQLQNVKV GWFLHTPFPS SEIYRILPVR
    210 220 230 240 250
    QEILKGVLSC DLVGFHTYDY ARHFLSSVQR VLNVNTLPNG VEYQGRFVNV
    260 270 280 290 300
    GAFPIGIDVD KFTDGLKKES VQKRIQQLKE TFKGCKIIVG VDRLDYIKGV
    310 320 330 340 350
    PQKLHAMEVF LNEHPEWRGK VVLVQVAVPS RGDVEEYQYL RSVVNELVGR
    360 370 380 390 400
    INGQFGTVEF VPIHFMHKSI PFEELISLYA VSDVCLVSST RDGMNLVSYE
    410 420 430 440 450
    YIACQEEKKG SLILSEFTGA AQSLNGAIIV NPWNTDDLSD AINEALTLPD
    460 470 480 490
    VKKEVNWEKL YKYISKYTSA FWGENFVHEL YSTSSSSTSS SATKN
    Length:495
    Mass (Da):56,148
    Last modified:July 1, 1993 - v2
    Checksum:i47325A0FA3554E3D
    GO

    Sequence cautioni

    The sequence AAA66891 differs from that shown. Reason: Frameshift at position 32.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti75K → M in CAA48296 (PubMed:1425702).Curated1
    Sequence conflicti101H → L in CAA48296 (PubMed:1425702).Curated1
    Sequence conflicti104G → W in CAA48296 (PubMed:1425702).Curated1
    Sequence conflicti129 – 130LA → FG in CAA43580 (PubMed:1315471).Curated2
    Sequence conflicti217T → S in CAA48296 (PubMed:1425702).Curated1
    Sequence conflicti288I → L in CAA48296 (PubMed:1425702).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J04450 Genomic DNA. Translation: AAA66891.1. Frameshift.
    X68214 mRNA. Translation: CAA48296.1.
    X68496 Genomic DNA. Translation: CAA48510.1.
    X67499 Genomic DNA. Translation: CAA47834.1.
    X61275 Genomic DNA. Translation: CAA43580.1.
    L21999 Genomic DNA. Translation: AAA53672.1.
    X78993 Genomic DNA. Translation: CAA55627.1. Sequence problems.
    X75891 Genomic DNA. Translation: CAA53485.1.
    AF061037 mRNA. Translation: AAC16974.1.
    AY598966 Genomic DNA. Translation: AAT27376.1.
    AY598964 Genomic DNA. Translation: AAT27374.1.
    Z35995 Genomic DNA. Translation: CAA85083.1.
    AY693147 Genomic DNA. Translation: AAT93166.1.
    BK006936 Genomic DNA. Translation: DAA07243.1.
    PIRiS34979.
    RefSeqiNP_009684.1. NM_001178474.1.

    Genome annotation databases

    EnsemblFungiiYBR126C; YBR126C; YBR126C.
    GeneIDi852423.
    KEGGisce:YBR126C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J04450 Genomic DNA. Translation: AAA66891.1. Frameshift.
    X68214 mRNA. Translation: CAA48296.1.
    X68496 Genomic DNA. Translation: CAA48510.1.
    X67499 Genomic DNA. Translation: CAA47834.1.
    X61275 Genomic DNA. Translation: CAA43580.1.
    L21999 Genomic DNA. Translation: AAA53672.1.
    X78993 Genomic DNA. Translation: CAA55627.1. Sequence problems.
    X75891 Genomic DNA. Translation: CAA53485.1.
    AF061037 mRNA. Translation: AAC16974.1.
    AY598966 Genomic DNA. Translation: AAT27376.1.
    AY598964 Genomic DNA. Translation: AAT27374.1.
    Z35995 Genomic DNA. Translation: CAA85083.1.
    AY693147 Genomic DNA. Translation: AAT93166.1.
    BK006936 Genomic DNA. Translation: DAA07243.1.
    PIRiS34979.
    RefSeqiNP_009684.1. NM_001178474.1.

    3D structure databases

    ProteinModelPortaliQ00764.
    SMRiQ00764.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi32827. 226 interactors.
    DIPiDIP-744N.
    IntActiQ00764. 26 interactors.
    MINTiMINT-618278.

    Protein family/group databases

    CAZyiGT20. Glycosyltransferase Family 20.

    PTM databases

    iPTMnetiQ00764.

    Proteomic databases

    MaxQBiQ00764.
    PRIDEiQ00764.
    TopDownProteomicsiQ00764.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYBR126C; YBR126C; YBR126C.
    GeneIDi852423.
    KEGGisce:YBR126C.

    Organism-specific databases

    EuPathDBiFungiDB:YBR126C.
    SGDiS000000330. TPS1.

    Phylogenomic databases

    GeneTreeiENSGT00550000075557.
    HOGENOMiHOG000191477.
    InParanoidiQ00764.
    KOiK00697.
    OMAiWLAYNEA.
    OrthoDBiEOG092C0LDC.

    Enzyme and pathway databases

    BioCyciMetaCyc:YBR126C-MONOMER.
    YEAST:YBR126C-MONOMER.
    BRENDAi2.4.1.15. 984.

    Miscellaneous databases

    PROiQ00764.

    Family and domain databases

    CDDicd03788. GT1_TPS. 1 hit.
    InterProiIPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiTPS1_YEAST
    AccessioniPrimary (citable) accession number: Q00764
    Secondary accession number(s): D6VQC3
    , Q01801, Q05168, Q6J5J4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: July 1, 1993
    Last modified: November 2, 2016
    This is version 167 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 10900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.