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Q00764 (TPS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit

EC=2.4.1.15
Alternative name(s):
General glucose sensor subunit 1
Glycogen metabolism control protein GLC6
Trehalose synthase complex catalytic subunit TPS1
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene names
Name:TPS1
Synonyms:BYP1, CIF1, FDP1, GGS1, GLC6, TSS1
Ordered Locus Names:YBR126C
ORF Names:YBR0922
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length495 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Synthase catalytic subunit of the trehalose synthase complex that catalyzes the production of trehalose from glucose-6-phosphate and UDP-glucose in a two step process. Can function independently of the complex. Ref.17

Catalytic activity

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Enzyme regulation

Activated by fructose 6-phosphate. Inorganic phosphate inhibits the synthase activity in the complex, but activates the synthase activity in the free monomeric form. Ref.16

Subunit structure

The trehalose synthase complex is composed of the two catalytic subunits TPS1 and TPS2 and at least one of the two regulatory subunits TPS3 or TSL1. Ref.18

Subcellular location

Cytoplasm Ref.19.

Induction

Either partial repression by glucose or induction by galactose. Induced by heat shock. Ref.16

Miscellaneous

Present with 10900 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyltransferase 20 family.

Biophysicochemical properties

Kinetic parameters:

KM=3.5 mM for D-glucose 6-phosphate Ref.15

KM=0.5 mM for UDP-glucose

Sequence caution

The sequence AAA66891.1 differs from that shown. Reason: Frameshift at position 32.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 495495Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit
PRO_0000122502

Experimental info

Mutagenesis2231H → Y in GLC6-1; low glycogen accumulation. Ref.6
Sequence conflict751K → M in CAA48296. Ref.2
Sequence conflict1011H → L in CAA48296. Ref.2
Sequence conflict1041G → W in CAA48296. Ref.2
Sequence conflict129 – 1302LA → FG in CAA43580. Ref.4
Sequence conflict2171T → S in CAA48296. Ref.2
Sequence conflict2881I → L in CAA48296. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q00764 [UniParc].

Last modified July 1, 1993. Version 2.
Checksum: 47325A0FA3554E3D

FASTA49556,148
        10         20         30         40         50         60 
MTTDNAKAQL TSSSGGNIIV VSNRLPVTIT KNSSTGQYEY AMSSGGLVTA LEGLKKTYTF 

        70         80         90        100        110        120 
KWFGWPGLEI PDDEKDQVRK DLLEKFNAVP IFLSDEIADL HYNGFSNSIL WPLFHYHPGE 

       130        140        150        160        170        180 
INFDENAWLA YNEANQTFTN EIAKTMNHND LIWVHDYHLM LVPEMLRVKI HEKQLQNVKV 

       190        200        210        220        230        240 
GWFLHTPFPS SEIYRILPVR QEILKGVLSC DLVGFHTYDY ARHFLSSVQR VLNVNTLPNG 

       250        260        270        280        290        300 
VEYQGRFVNV GAFPIGIDVD KFTDGLKKES VQKRIQQLKE TFKGCKIIVG VDRLDYIKGV 

       310        320        330        340        350        360 
PQKLHAMEVF LNEHPEWRGK VVLVQVAVPS RGDVEEYQYL RSVVNELVGR INGQFGTVEF 

       370        380        390        400        410        420 
VPIHFMHKSI PFEELISLYA VSDVCLVSST RDGMNLVSYE YIACQEEKKG SLILSEFTGA 

       430        440        450        460        470        480 
AQSLNGAIIV NPWNTDDLSD AINEALTLPD VKKEVNWEKL YKYISKYTSA FWGENFVHEL 

       490 
YSTSSSSTSS SATKN 

« Hide

References

« Hide 'large scale' references
[1]"A conserved gene encoding the 57-kDa subunit of the yeast vacuolar H+-ATPase."
Nelson H., Mandiyan S., Nelson N.
J. Biol. Chem. 264:1775-1778(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Characterization of the 56-kDa subunit of yeast trehalose-6-phosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation."
Bell W., Klaassen P., Ohnacker M., Boller T., Herweijer M., Schoppink P., van der Zee P., Wiemken A.
Eur. J. Biochem. 209:951-959(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C13-ABYS86.
[3]"A yeast gene for trehalose-6-phosphate synthase and its complementation of an Escherichia coli otsA mutant."
McDougall J., Kaasen I., Stroem A.R.
FEMS Microbiol. Lett. 107:25-30(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Molecular cloning of CIF1, a yeast gene necessary for growth on glucose."
Gonzales M.I., Stucka R., Blazquez M.A., Feldmann H., Gancedo C.
Yeast 8:183-192(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C836.
[5]"Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae."
van Aelst L., Hohmann S., Bulaya B., de Koning W., Sierkstra L., Neves M.J., Luyten K., Alijo R., Ramos J., Coccetti P., Martegani E., de Magalhaes-Rocha N.M., Brandao R.L., van Dijck P., Vanhalewyn M., Durnez P., Jans A.W.H., Thevelein J.M.
Mol. Microbiol. 8:927-943(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"Characterization of glycogen-deficient glc mutants of Saccharomyces cerevisiae."
Cannon J.F., Pringle J.R., Fiechter A., Khalil M.
Genetics 136:485-503(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-223.
[7]"Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae."
Vuorio O.E., Kalkkinen N., Londesborough J.
Eur. J. Biochem. 216:849-861(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 204508 / S288c.
[8]"cDNA cloning, sequence analysis of trehalose-6-phosphate synthase gene from Saccharomyces cerevisiae AS2.1416."
Dong Z.Y., Duan Y.K., Chen H.M., Jin C., Zhang S.Z.
Sheng Wu Gong Cheng Xue Bao 16:408-410(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: AS2.1416.
[9]"Research on the mechanism of osmotolerance and thermotolerance of yeast."
Ma X., Guo Y., Zhang F., Yu H., Kuang J., Yao J., Li Z., He G.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: FHS and WFB.
[10]"The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes."
Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F., Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M., Herbert C.J.
Yeast 10:S1-S11(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[11]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[12]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[13]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[14]"Analysis of a 70 kb region on the right arm of yeast chromosome II."
Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 291-495.
Strain: ATCC 204508 / S288c.
[15]"Characterization of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase of Saccharomyces cerevisiae."
Vandercammen A., Francois J., Hers H.-G.
Eur. J. Biochem. 182:613-620(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
[16]"Purification of trehalose synthase from baker's yeast. Its temperature-dependent activation by fructose 6-phosphate and inhibition by phosphate."
Londesborough J., Vuorio O.E.
Eur. J. Biochem. 216:841-848(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[17]"Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock."
Reinders A., Buerckert N., Hohmann S., Thevelein J.M., Boller T., Wiemken A., De Virgilio C.
Mol. Microbiol. 24:687-695(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TPS2; TPS3 AND TSL1.
[18]"Composition and functional analysis of the Saccharomyces cerevisiae trehalose synthase complex."
Bell W., Sun W., Hohmann S., Wera S., Reinders A., De Virgilio C., Wiemken A., Thevelein J.M.
J. Biol. Chem. 273:33311-33319(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[19]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[20]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[21]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04450 Genomic DNA. Translation: AAA66891.1. Frameshift.
X68214 mRNA. Translation: CAA48296.1.
X68496 Genomic DNA. Translation: CAA48510.1.
X67499 Genomic DNA. Translation: CAA47834.1.
X61275 Genomic DNA. Translation: CAA43580.1.
L21999 Genomic DNA. Translation: AAA53672.1.
X78993 Genomic DNA. Translation: CAA55627.1. Sequence problems.
X75891 Genomic DNA. Translation: CAA53485.1.
AF061037 mRNA. Translation: AAC16974.1.
AY598966 Genomic DNA. Translation: AAT27376.1.
AY598964 Genomic DNA. Translation: AAT27374.1.
Z35995 Genomic DNA. Translation: CAA85083.1.
AY693147 Genomic DNA. Translation: AAT93166.1.
BK006936 Genomic DNA. Translation: DAA07243.1.
PIRS34979.
RefSeqNP_009684.1. NM_001178474.1.

3D structure databases

ProteinModelPortalQ00764.
SMRQ00764. Positions 18-480.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32827. 221 interactions.
DIPDIP-744N.
IntActQ00764. 26 interactions.
MINTMINT-618278.
STRING4932.YBR126C.

Protein family/group databases

CAZyGT20. Glycosyltransferase Family 20.

Proteomic databases

PaxDbQ00764.
PeptideAtlasQ00764.
PRIDEQ00764.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR126C; YBR126C; YBR126C.
GeneID852423.
KEGGsce:YBR126C.

Organism-specific databases

CYGDYBR126c.
SGDS000000330. TPS1.

Phylogenomic databases

eggNOGCOG0380.
GeneTreeENSGT00550000075557.
HOGENOMHOG000191477.
KOK00697.
OMAIEFMPIH.
OrthoDBEOG76QFS6.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-563.
YEAST:YBR126C-MONOMER.

Gene expression databases

GenevestigatorQ00764.

Family and domain databases

InterProIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetSearch...

Other

NextBio971291.
PROQ00764.

Entry information

Entry nameTPS1_YEAST
AccessionPrimary (citable) accession number: Q00764
Secondary accession number(s): D6VQC3 expand/collapse secondary AC list , Q01801, Q05168, Q6J5J4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families