Reviewed, UniProtKB/Swiss-Prot Q00757 (CHSB_EMENI)

Names and origin

Protein names

Recommended name:
    Chitin synthase B
    EC=2.4.1.16
Alternative name(s):
    Chitin-UDP acetyl-glucosaminyl transferase B
    Class-III chitin synthase B

Gene names

Name:	chsB
ORF Names:	AN2523

Organism

Emericella nidulans (Aspergillus nidulans) [Complete proteome]

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	916 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class III subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 916	916	Chitin synthase B		PRO_0000193692
Regions
	Transmembrane	544 – 561	18	Potential
	Transmembrane	588 – 608	21	Potential
	Transmembrane	629 – 649	21	Potential
	Transmembrane	664 – 684	21	Potential
	Transmembrane	716 – 736	21	Potential
	Transmembrane	845 – 865	21	Potential
	Transmembrane	884 – 904	21	Potential
Experimental info
	Sequence conflict	136 – 137	2	AG → RR in BAA04807. Ref.1
	Sequence conflict	136 – 137	2	AG → RR in BAA11845. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q00757-1 [UniParc]. Last modified May 26, 2009. Version 2. Checksum: 622356DC99AA4B5E	FASTA	916	101,882
	10 20 30 40 50 60 MAYHGSGPQS PGEHTYDDGH QLRDLSHSNT SYEEEASHGL LSSQQSPFAG PFDDPHQQRG 70 80 90 100 110 120 LTASPVQRPT SGYSLTESYA PDAAYHDPYS ANQSVYSGHS ENPAAAFGVP GRVASPYARS 130 140 150 160 170 180 ETSSTEAWRQ RQAGAAGGGN GLRRYATRKV KLVQGSVLSV DYPVPSAIQN AIQAKYRNDL 190 200 210 220 230 240 EGGSEEFTHM RYTAATCDPN EFTLHNGYNL RPAMYNRHTE LLIAITYYNE DKTLTARTLH 250 260 270 280 290 300 GVMQNIRDIV NLKKSEFWNK GGPAWQKIVV CLVFDGIDPC DKDTLDVLAT VGIYQDGVMK 310 320 330 340 350 360 RDVDGKETVA HIFEYTTQLS VTPNQQLIRP TDDGPSTLPP VQMMFCLKQK NSKKINSHRW 370 380 390 400 410 420 LFNAFGRILN PEVCILLDAG TKPGPKSLLY LWEAFYNDKD LGGACGEIHA MLGKGWKKLL 430 440 450 460 470 480 NPLVAAQNFE YKISNILDKP LESSFGYVSV LPGAFSAYRF RAIMGRPLEQ YFHGDHTLSK 490 500 510 520 530 540 QLGKKGIEGM NIFKKNMFLA EDRILCFELV AKAGSKWHLS YVKASKGETD VPEGAPEFIS 550 560 570 580 590 600 QRRRWLNGSF AAGIYSLMHF GRMYKSGHNI VRMFFLHLQM LYNWFSTFLT WFSLASYWLT 610 620 630 640 650 660 TSVIMDLVGT PSSSNGYTAF PFGKTATPII NTLVKYIYLA FLLLQFILAL GNRPKGSKLS 670 680 690 700 710 720 YLASFVAFGI IQLYVVVDAL YLVVRAFTGG APMDFNTDDG IGAFLSSFFG SSGAGIIIIA 730 740 750 760 770 780 LAATFGLYFV ASFMYLDPWH MFTSFPAYMA VQSSYINILN VYAFSNWHDV SWGTKGSDKA 790 800 810 820 830 840 DALPSAKTTG GKGEEAVIEE IDKPQADIDS QFEATVKRAL TPYVPPEEKE EKSLDDSYKS 850 860 870 880 890 900 FRTRLVTLWL FSNGLLAVCI TSEGLDKFGF TNTSTERTSR FFQALLWSNA VVALIRFIGA 910 TWFLGKTGLL CCFARR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of two chitin synthase genes from Aspergillus nidulans." Yanai K., Kojima N., Takaya N., Horiuchi H., Ohta A., Takagi M. Biosci. Biotechnol. Biochem. 58:1828-1835(1994) [PubMed: 7765508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FGSC 89.
[2]	"Aspergillus nidulans chitin synthase B gene." Tatsuno K. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: FGSC 89.
[3]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D21269 Genomic DNA. Translation: BAA04807.1. D83216 mRNA. Translation: BAA11845.1. AACD01000043 Genomic DNA. Translation: EAA64628.1.
PIR	JC2315.
RefSeq	XP_660127.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	2874737.
KEGG	ani:AN2523.2.
Phylogenomic databases
PhylomeDB	Q00757.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHSB_EMENI

Accession

Primary (citable) accession number: Q00757
Secondary accession number(s): P87326, Q5BAA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 26, 2009
Last modified:	January 19, 2010
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)