Reviewed, UniProtKB/Swiss-Prot Q00756 (PPR3A_RABIT)

Names and origin

Protein names

Recommended name:
    Protein phosphatase 1 regulatory subunit 3A
Alternative name(s):
    Protein phosphatase 1 glycogen-associated regulatory subunit
    Protein phosphatase type-1 glycogen targeting subunit
    RG1

Gene names

Name:	PPP1R3A
Synonyms:	PP1G

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	1109 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Plays an important role in glycogen synthesis but is not essential for insulin activation of glycogen synthase By similarity.
Subunit structure	Interacts with PPP1CC catalytic subunit of PP1, and associates with glycogen By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Skeletal muscle, diaphragm and cardiac muscle. Ref.1
Domain	The CBM21 domain is known to be involved in the localization to glycogen and is characteristic of some regulatory subunit of phosphatase complexes.
Post-translational modification	Phosphorylation at Ser-48 by ISPK stimulates the dephosphorylation of glycogen synthase and phosphorylase kinase.
Sequence similarities	Contains 1 CBM21 (carbohydrate binding type-21) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycogen metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1109	1109	Protein phosphatase 1 regulatory subunit 3A		PRO_0000071502
Regions
	Transmembrane	1066 – 1086	21	Potential
	Domain	124 – 232	109	CBM21
	Motif	64 – 67	4	PP1-binding motif
Amino acid modifications
	Modified residue	40	1	Phosphoserine; by GSK3 Ref.2
	Modified residue	44	1	Phosphoserine; by GSK3 Ref.2
	Modified residue	48	1	Phosphoserine; by PKA and ISPK Ref.2 Ref.3
	Modified residue	67	1	Phosphoserine; by PKA Ref.2
	Modified residue	263	1	Phosphoserine By similarity
	Modified residue	758	1	Phosphothreonine By similarity
Natural variations
	Natural variant	311	1	T → M Ref.1
	Natural variant	413	1	N → K Ref.1
Experimental info
	Sequence conflict	26	1	C → Y AA sequence Ref.1
	Sequence conflict	183	1	C → S AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q00756-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1CD9FB8F47890226	FASTA	1,109	124,170
	10 20 30 40 50 60 MEPSEVPGQN SKDNFLEVPN LSDSLCEDEE VKAIFKPGFS PQPSRRGSES SEEVYVHTAS 70 80 90 100 110 120 SGGRRVSFAD NFGFNLVSVK EFDTWELPSV STTFELGKDA FQTEEYVLSP LFDLPASKED 130 140 150 160 170 180 LMQQLQVQKA MLESTEYVPG STSMKGIIRV LNISFEKLVY VRMSLDDWQT HYDILAEYVP 190 200 210 220 230 240 NSCDGETDQF SFKISLVPPY QKDGSKVEFC IRYETSVGTF WSNNNGTNYT LVCQKKEPEP 250 260 270 280 290 300 EPGKPLEEAP SKQKKGCLKV KSSKEESSET SEENNFENSK IADTYIPTIV CSHEEKEDLK 310 320 330 340 350 360 SSYQNVKDVN TEHDEHNEKE LELMINQRLI RTRCAASEYG KNTLSSDPSN IPNKPEELQK 370 380 390 400 410 420 NQSHSEACTD LSQRLLSPGS SAESSLKGDF YHTEKYSSGN ESSHQPSDMG EINPSLGGTT 430 440 450 460 470 480 SDGSVQLHIS SKEILDDNAN PAHGSGRGEI SCSFPGQLKA SNLNKKYEGG AENSEMKDCE 490 500 510 520 530 540 CLPRDVHLKA SDYFKKSTEN RPSEEDYGTS KDNKEKRIQL DVDEKTSKNF RSIFYDQERN 550 560 570 580 590 600 VGHLEITVEG IEASDRDLTS LPTKDTTIPT WAIMEDTFHS SRTPLGREEA VLTTPEHDLS 610 620 630 640 650 660 SSEGTILGGL TGGVCSPRNG NVLKNDYLFQ VEKRKSGWIN PEDQNKDTQH QQSWNVLESQ 670 680 690 700 710 720 EKARGSKTNI AEQIKEQVDC EDMWEKRDNT GSLKATPAEA LFTCQEAEHC ELSPLADHGI 730 740 750 760 770 780 PGKAEAGTAY IIKTTSETTP ESMSAGEKAI IAKLPQETAR SDRPMEVKET AFDPHEGRND 790 800 810 820 830 840 DSHYTLCQRD TVGVIDDNGV EKESHLDICN ARLDEMRKEE AMSMHSPGKM RDREKLGIGN 850 860 870 880 890 900 ITSVEESSQV IANNEKATSR LDLHLEMPSA DKKIFPENRD LGQVQELSKK TDIDNTVHSA 910 920 930 940 950 960 FNSDTNRASR DDSLLSSHHT ETSVLSCEQA NAVKNTVTTT ALQTSATESE YNCSPTRETQ 970 980 990 1000 1010 1020 GQPASKPEEV SRGSRRVTSE TRKEKCVGQM FQSGECNVEM SQGPMILVSE SRENVERERH 1030 1040 1050 1060 1070 1080 ENEGLINSGD KEFESSASSS LPVQETQDQS NESLLSKYTN SKIPYFLLFL MFLVTVYHYD 1090 1100 LMIGLAFYLF SLYWLYWEEG RQKESVKKK

References

[1]	"Molecular cloning and expression of the regulatory (RG1) subunit of the glycogen-associated protein phosphatase." Tang P.M., Bondor J.A., Swiderek K.M., DePaoli-Roach A.A. J. Biol. Chem. 266:15782-15789(1991) [PubMed: 1651919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 13-52; 59-68; 75-118; 163-193; 540-561; 565-580 AND 625-632, TISSUE SPECIFICITY, VARIANTS MET-311 AND LYS-413. Strain: New Zealand. Tissue: Skeletal muscle.
[2]	"Multisite phosphorylation of the glycogen-binding subunit of protein phosphatase-1G by cyclic AMP-dependent protein kinase and glycogen synthase kinase-3." Dent P., Campbell D.G., Hubbard M.J., Cohen P. FEBS Lett. 248:67-72(1989) [PubMed: 2542090] [Abstract] Cited for: PROTEIN SEQUENCE OF 33-46, PHOSPHORYLATION AT SER-40; SER-44; SER-48 AND SER-67.
[3]	"The molecular mechanism by which insulin stimulates glycogen synthesis in mammalian skeletal muscle." Dent P., Lavoinne A., Nakielny S., Caudwell F.B., Watt P., Cohen P. Nature 348:302-308(1990) [PubMed: 2123524] [Abstract] Cited for: PHOSPHORYLATION AT SER-48.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M65109 mRNA. Translation: AAA31462.1.
PIR	A40801.
RefSeq	NP_001075772.1.
UniGene	Ocu.1960
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM21. Carbohydrate-Binding Module Family 21.
Genome annotation databases
Ensembl	ENSOCUG00000000303. Oryctolagus cuniculus. [Contig view]
GeneID	100009140.
Phylogenomic databases
HOVERGEN	Q00756.
Family and domain databases
InterPro	IPR005036. CBM_21. [Graphical view]
Pfam	PF03370. CBM_21. 1 hit. [Graphical view]
PROSITE	PS51159. CBM21. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPR3A_RABIT

Accession

Primary (citable) accession number: Q00756

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	November 1, 1996
Last modified:	April 14, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)