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Protein

Protein phosphatase 1 regulatory subunit 3A

Gene

PPP1R3A

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Plays an important role in glycogen synthesis but is not essential for insulin activation of glycogen synthase (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 3A
Alternative name(s):
Protein phosphatase 1 glycogen-associated regulatory subunit
Protein phosphatase type-1 glycogen targeting subunit
Short name:
RG1
Gene namesi
Name:PPP1R3A
Synonyms:PP1G
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Chromosome 7

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1066 – 108621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11091109Protein phosphatase 1 regulatory subunit 3APRO_0000071502Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401Phosphoserine; by GSK31 Publication
Modified residuei44 – 441Phosphoserine; by GSK31 Publication
Modified residuei48 – 481Phosphoserine; by PKA and ISPK2 Publications
Modified residuei51 – 511PhosphoserineBy similarity
Modified residuei58 – 581PhosphothreonineBy similarity
Modified residuei67 – 671Phosphoserine; by PKA1 Publication
Modified residuei843 – 8431PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation at Ser-48 by ISPK stimulates the dephosphorylation of glycogen synthase and phosphorylase kinase.2 Publications

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiQ00756.

Expressioni

Tissue specificityi

Skeletal muscle, diaphragm and cardiac muscle.1 Publication

Interactioni

Subunit structurei

Interacts with PPP1CC catalytic subunit of PP1, and associates with glycogen.By similarity

Protein-protein interaction databases

IntActiQ00756. 2 interactions.
MINTiMINT-6369057.
STRINGi9986.ENSOCUP00000000266.

Structurei

Secondary structure

1
1109
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi104 – 1063Combined sources
Beta strandi108 – 1103Combined sources
Helixi118 – 1258Combined sources
Beta strandi130 – 1378Combined sources
Beta strandi144 – 1518Combined sources
Beta strandi155 – 16915Combined sources
Beta strandi185 – 1939Combined sources
Turni198 – 2003Combined sources
Beta strandi207 – 2159Combined sources
Beta strandi218 – 2225Combined sources
Beta strandi231 – 2355Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M83NMR-A102-237[»]
ProteinModelPortaliQ00756.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 232109CBM21PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 674PP1-binding motif

Domaini

The CBM21 domain is known to be involved in the localization to glycogen and is characteristic of some regulatory subunit of phosphatase complexes.

Sequence similaritiesi

Contains 1 CBM21 (carbohydrate binding type-21) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3986. Eukaryota.
ENOG4111FT1. LUCA.
GeneTreeiENSGT00530000062978.
HOGENOMiHOG000115667.
HOVERGENiHBG053657.
InParanoidiQ00756.
KOiK07189.
OMAiLFTCQET.
OrthoDBiEOG769ZKR.
TreeFamiTF105537.

Family and domain databases

InterProiIPR005036. CBM21_dom.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
[Graphical view]
PROSITEiPS51159. CBM21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00756-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPSEVPGQN SKDNFLEVPN LSDSLCEDEE VKAIFKPGFS PQPSRRGSES
60 70 80 90 100
SEEVYVHTAS SGGRRVSFAD NFGFNLVSVK EFDTWELPSV STTFELGKDA
110 120 130 140 150
FQTEEYVLSP LFDLPASKED LMQQLQVQKA MLESTEYVPG STSMKGIIRV
160 170 180 190 200
LNISFEKLVY VRMSLDDWQT HYDILAEYVP NSCDGETDQF SFKISLVPPY
210 220 230 240 250
QKDGSKVEFC IRYETSVGTF WSNNNGTNYT LVCQKKEPEP EPGKPLEEAP
260 270 280 290 300
SKQKKGCLKV KSSKEESSET SEENNFENSK IADTYIPTIV CSHEEKEDLK
310 320 330 340 350
SSYQNVKDVN TEHDEHNEKE LELMINQRLI RTRCAASEYG KNTLSSDPSN
360 370 380 390 400
IPNKPEELQK NQSHSEACTD LSQRLLSPGS SAESSLKGDF YHTEKYSSGN
410 420 430 440 450
ESSHQPSDMG EINPSLGGTT SDGSVQLHIS SKEILDDNAN PAHGSGRGEI
460 470 480 490 500
SCSFPGQLKA SNLNKKYEGG AENSEMKDCE CLPRDVHLKA SDYFKKSTEN
510 520 530 540 550
RPSEEDYGTS KDNKEKRIQL DVDEKTSKNF RSIFYDQERN VGHLEITVEG
560 570 580 590 600
IEASDRDLTS LPTKDTTIPT WAIMEDTFHS SRTPLGREEA VLTTPEHDLS
610 620 630 640 650
SSEGTILGGL TGGVCSPRNG NVLKNDYLFQ VEKRKSGWIN PEDQNKDTQH
660 670 680 690 700
QQSWNVLESQ EKARGSKTNI AEQIKEQVDC EDMWEKRDNT GSLKATPAEA
710 720 730 740 750
LFTCQEAEHC ELSPLADHGI PGKAEAGTAY IIKTTSETTP ESMSAGEKAI
760 770 780 790 800
IAKLPQETAR SDRPMEVKET AFDPHEGRND DSHYTLCQRD TVGVIDDNGV
810 820 830 840 850
EKESHLDICN ARLDEMRKEE AMSMHSPGKM RDREKLGIGN ITSVEESSQV
860 870 880 890 900
IANNEKATSR LDLHLEMPSA DKKIFPENRD LGQVQELSKK TDIDNTVHSA
910 920 930 940 950
FNSDTNRASR DDSLLSSHHT ETSVLSCEQA NAVKNTVTTT ALQTSATESE
960 970 980 990 1000
YNCSPTRETQ GQPASKPEEV SRGSRRVTSE TRKEKCVGQM FQSGECNVEM
1010 1020 1030 1040 1050
SQGPMILVSE SRENVERERH ENEGLINSGD KEFESSASSS LPVQETQDQS
1060 1070 1080 1090 1100
NESLLSKYTN SKIPYFLLFL MFLVTVYHYD LMIGLAFYLF SLYWLYWEEG

RQKESVKKK
Length:1,109
Mass (Da):124,170
Last modified:November 1, 1996 - v1
Checksum:i1CD9FB8F47890226
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261C → Y AA sequence (PubMed:1651919).Curated
Sequence conflicti183 – 1831C → S AA sequence (PubMed:1651919).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti311 – 3111T → M.1 Publication
Natural varianti413 – 4131N → K.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65109 mRNA. Translation: AAA31462.1.
PIRiA40801.
RefSeqiNP_001075772.1. NM_001082303.1.
UniGeneiOcu.1960.

Genome annotation databases

EnsembliENSOCUT00000000303; ENSOCUP00000000266; ENSOCUG00000000303.
GeneIDi100009140.
KEGGiocu:100009140.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65109 mRNA. Translation: AAA31462.1.
PIRiA40801.
RefSeqiNP_001075772.1. NM_001082303.1.
UniGeneiOcu.1960.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M83NMR-A102-237[»]
ProteinModelPortaliQ00756.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ00756. 2 interactions.
MINTiMINT-6369057.
STRINGi9986.ENSOCUP00000000266.

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.

PTM databases

iPTMnetiQ00756.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSOCUT00000000303; ENSOCUP00000000266; ENSOCUG00000000303.
GeneIDi100009140.
KEGGiocu:100009140.

Organism-specific databases

CTDi5506.

Phylogenomic databases

eggNOGiKOG3986. Eukaryota.
ENOG4111FT1. LUCA.
GeneTreeiENSGT00530000062978.
HOGENOMiHOG000115667.
HOVERGENiHBG053657.
InParanoidiQ00756.
KOiK07189.
OMAiLFTCQET.
OrthoDBiEOG769ZKR.
TreeFamiTF105537.

Family and domain databases

InterProiIPR005036. CBM21_dom.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
[Graphical view]
PROSITEiPS51159. CBM21. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and expression of the regulatory (RG1) subunit of the glycogen-associated protein phosphatase."
    Tang P.M., Bondor J.A., Swiderek K.M., DePaoli-Roach A.A.
    J. Biol. Chem. 266:15782-15789(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 13-52; 59-68; 75-118; 163-193; 540-561; 565-580 AND 625-632, TISSUE SPECIFICITY, VARIANTS MET-311 AND LYS-413.
    Strain: New Zealand.
    Tissue: Skeletal muscle.
  2. "Multisite phosphorylation of the glycogen-binding subunit of protein phosphatase-1G by cyclic AMP-dependent protein kinase and glycogen synthase kinase-3."
    Dent P., Campbell D.G., Hubbard M.J., Cohen P.
    FEBS Lett. 248:67-72(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 33-46, PHOSPHORYLATION AT SER-40; SER-44; SER-48 AND SER-67.
  3. "The molecular mechanism by which insulin stimulates glycogen synthesis in mammalian skeletal muscle."
    Dent P., Lavoinne A., Nakielny S., Caudwell F.B., Watt P., Cohen P.
    Nature 348:302-308(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-48.

Entry informationi

Entry nameiPPR3A_RABIT
AccessioniPrimary (citable) accession number: Q00756
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: November 1, 1996
Last modified: January 20, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.