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Q00748

- MDR65_DROME

UniProt

Q00748 - MDR65_DROME

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Protein

Multidrug resistance protein homolog 65

Gene

Mdr65

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi440 – 4478ATP 1PROSITE-ProRule annotation
Nucleotide bindingi1094 – 11018ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. efflux transmembrane transporter activity Source: FlyBase
  3. xenobiotic-transporting ATPase activity Source: FlyBase

GO - Biological processi

  1. drug transmembrane transport Source: GOC
  2. establishment of glial blood-brain barrier Source: FlyBase
  3. response to methotrexate Source: FlyBase
  4. transmembrane transport Source: RefGenome
  5. xenobiotic transport Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_180846. PPARA activates gene expression.
REACT_184368. Abacavir transmembrane transport.
REACT_240183. Recycling of bile acids and salts.
REACT_245482. ABC-family proteins mediated transport.
REACT_263220. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein homolog 65 (EC:3.6.3.44)
Alternative name(s):
P-glycoprotein 65
Gene namesi
Name:Mdr65
ORF Names:CG10181
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0004513. Mdr65.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei49 – 6921Helical; Name=1PROSITE-ProRule annotationAdd
BLAST
Topological domaini70 – 11849ExtracellularSequence AnalysisAdd
BLAST
Transmembranei119 – 14729Helical; Name=2PROSITE-ProRule annotationAdd
BLAST
Topological domaini148 – 19447CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei195 – 21521Helical; Name=3PROSITE-ProRule annotationAdd
BLAST
Topological domaini216 – 2238ExtracellularSequence Analysis
Transmembranei224 – 24219Helical; Name=4PROSITE-ProRule annotationAdd
BLAST
Topological domaini243 – 30260CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei303 – 32321Helical; Name=5PROSITE-ProRule annotationAdd
BLAST
Topological domaini324 – 34118ExtracellularSequence AnalysisAdd
BLAST
Transmembranei342 – 36221Helical; Name=6PROSITE-ProRule annotationAdd
BLAST
Topological domaini363 – 731369CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei732 – 75322Helical; Name=7PROSITE-ProRule annotationAdd
BLAST
Topological domaini754 – 77623ExtracellularSequence AnalysisAdd
BLAST
Transmembranei777 – 79822Helical; Name=8PROSITE-ProRule annotationAdd
BLAST
Topological domaini799 – 85254CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei853 – 87321Helical; Name=9PROSITE-ProRule annotationAdd
BLAST
Topological domaini874 – 8741ExtracellularSequence Analysis
Transmembranei875 – 89420Helical; Name=10PROSITE-ProRule annotationAdd
BLAST
Topological domaini895 – 95662CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei957 – 97721Helical; Name=11PROSITE-ProRule annotationAdd
BLAST
Topological domaini978 – 99316ExtracellularSequence AnalysisAdd
BLAST
Transmembranei994 – 101421Helical; Name=12PROSITE-ProRule annotationAdd
BLAST
Topological domaini1015 – 1302288CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. ATP-binding cassette (ABC) transporter complex Source: FlyBase
  2. plasma membrane Source: FlyBase
  3. pleated septate junction Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13021302Multidrug resistance protein homolog 65PRO_0000093348Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi103 – 1031N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ00748.
PRIDEiQ00748.

Expressioni

Gene expression databases

BgeeiQ00748.
ExpressionAtlasiQ00748. differential.

Interactioni

Protein-protein interaction databases

BioGridi64179. 8 interactions.
DIPiDIP-17575N.
IntActiQ00748. 2 interactions.
MINTiMINT-288870.

Structurei

3D structure databases

ProteinModelPortaliQ00748.
SMRiQ00748. Positions 35-690, 717-1300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 369322ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini405 – 641237ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini732 – 1020289ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1059 – 1298240ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1132.
GeneTreeiENSGT00530000062896.
InParanoidiQ00748.
OMAiKPNFFRT.
OrthoDBiEOG7Z3F4H.
PhylomeDBiQ00748.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 3 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00748-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERDEVSTSS SEGKSQEEAP MAEGLEPTEP IAFLKLFRFS TYGEIGWLFF
60 70 80 90 100
GFIMCCIKAL TLPAVVIIYS EFTSMLVDRA MQFGTSSNVH ALPLFGGGKT
110 120 130 140 150
LTNASREENN EALYDDSISY GILLTIASVV MFISGIFSVD VFNMVALRQV
160 170 180 190 200
TRMRIKLFSS VIRQDIGWHD LASKQNFTQS MVDDVEKIRD GISEKVGHFV
210 220 230 240 250
YLVVGFIITV AISFSYGWKL TLAVSSYIPL VILLNYYVAK FQGKLTAREQ
260 270 280 290 300
ESYAGAGNLA EEILSSIRTV VSFGGEKSEV QRYENFLVPA RKASQWKGAF
310 320 330 340 350
SGLSDAVLKS MLYLSCAGAF WYGVNLIIDD RNVENKEYTP AILMIAFFGI
360 370 380 390 400
IVGADNIART APFLESFATA RGCATNLFKV IDLTSKIDPL STDGKLLNYG
410 420 430 440 450
LRGDVEFQDV FFRYPSRPEV IVHRGLNIRI RAGQTVALVG SSGCGKSTCV
460 470 480 490 500
QLLQRFYDPV FGSVLLDDLD IRKYNIQWLR SNIAVVGQEP VLFLGTIAQN
510 520 530 540 550
ISYGKPGATQ KEIEAAATQA GAHEFITNLP ESYRSMIGER GSQLSGGQKQ
560 570 580 590 600
RIAIARALIQ NPKILLLDEA TSALDYQSEK QVQQALDLAS KGRTTIVVSH
610 620 630 640 650
RLSAIRGADK IVFIHDGKVL EEGSHDDLMA LEGAYYNMVR AGDINMPDEV
660 670 680 690 700
EKEDSIEDTK QKSLALFEKS FETSPLNFEK GQKNSVQFEE PIIKALIKDT
710 720 730 740 750
NAQSAEAPPE KPNFFRTFSR ILQLAKQEWC YLILGTISAV AVGFLYPAFA
760 770 780 790 800
VIFGEFYAAL AEKDPEDALR RTAVLSWACL GLAFLTGLVC FLQTYLFNYA
810 820 830 840 850
GIWLTTRMRA MTFNAMVNQE VGWFDDENNS VGALSARLSG EAVDIQGAIG
860 870 880 890 900
YPLSGMIQAL SNFISSVSVA MYYNWKLALL CLANCPIIVG SVILEAKMMS
910 920 930 940 950
NAVVREKQVI EEACRIATES ITNIRTVAGL RREADVIREY TEEIQRVEVL
960 970 980 990 1000
IRQKLRWRGV LNSTMQASAF FAYAVALCYG GVLVSEGQLP FQDIIKVSET
1010 1020 1030 1040 1050
LLYGSMMLAQ SLAFTPAFSA ALIAGHRLFQ ILDRKPKIQS PMGTIKNTLA
1060 1070 1080 1090 1100
KQLNLFEGVR YRGIQFRYPT RPDAKILNGL DLEVLKGQTV ALVGHSGCGK
1110 1120 1130 1140 1150
STCVQLLQRY YDPDEGTIHI DHDDIQHDLT LDGVRTKLGI VSQEPTLFER
1160 1170 1180 1190 1200
SIAENIAYGD NRRSVSMVEI IAAAKSANAH SFIISLPNGY DTRMGARGTQ
1210 1220 1230 1240 1250
LSGGQKQRIA IARALVRNPK ILLLDEATSA LDLQSEQLVQ QALDTACSGR
1260 1270 1280 1290 1300
TCIVIAHRLS TVQNADVICV IQNGQVVEQG NHMQLISQGG IYAKLHKTQK

DH
Length:1,302
Mass (Da):143,784
Last modified:November 2, 2001 - v2
Checksum:i39A7BCABFA31924A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti369 – 3691T → S in AAA28680. (PubMed:2072901)Curated
Sequence conflicti678 – 6781F → L in AAA28680. (PubMed:2072901)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59077 mRNA. Translation: AAA28680.1.
AF251287 Genomic DNA. Translation: AAF69147.1.
AF251286 Genomic DNA. Translation: AAF69146.1.
AE014296 Genomic DNA. Translation: AAF50669.1.
PIRiB41249.
RefSeqiNP_476831.1. NM_057483.4.
UniGeneiDm.4740.

Genome annotation databases

EnsemblMetazoaiFBtr0077011; FBpp0076719; FBgn0004513.
GeneIDi38726.
KEGGidme:Dmel_CG10181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59077 mRNA. Translation: AAA28680.1 .
AF251287 Genomic DNA. Translation: AAF69147.1 .
AF251286 Genomic DNA. Translation: AAF69146.1 .
AE014296 Genomic DNA. Translation: AAF50669.1 .
PIRi B41249.
RefSeqi NP_476831.1. NM_057483.4.
UniGenei Dm.4740.

3D structure databases

ProteinModelPortali Q00748.
SMRi Q00748. Positions 35-690, 717-1300.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 64179. 8 interactions.
DIPi DIP-17575N.
IntActi Q00748. 2 interactions.
MINTi MINT-288870.

Proteomic databases

PaxDbi Q00748.
PRIDEi Q00748.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0077011 ; FBpp0076719 ; FBgn0004513 .
GeneIDi 38726.
KEGGi dme:Dmel_CG10181.

Organism-specific databases

CTDi 38726.
FlyBasei FBgn0004513. Mdr65.

Phylogenomic databases

eggNOGi COG1132.
GeneTreei ENSGT00530000062896.
InParanoidi Q00748.
OMAi KPNFFRT.
OrthoDBi EOG7Z3F4H.
PhylomeDBi Q00748.

Enzyme and pathway databases

Reactomei REACT_180846. PPARA activates gene expression.
REACT_184368. Abacavir transmembrane transport.
REACT_240183. Recycling of bile acids and salts.
REACT_245482. ABC-family proteins mediated transport.
REACT_263220. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.

Miscellaneous databases

GenomeRNAii 38726.
NextBioi 810073.

Gene expression databases

Bgeei Q00748.
ExpressionAtlasi Q00748. differential.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 3 hits.
PROSITEi PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of Drosophila multidrug resistance gene homologs."
    Wu C.-T., Budding M., Griffin M.S., Croop J.M.
    Mol. Cell. Biol. 11:3940-3948(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Head.
  2. "Genetics of alpha-amanitin resistance in a natural population of Drosophila melanogaster."
    Begun D.J., Whitley P.
    Heredity 85:184-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: WSIII25 and WSIII27.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. "Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster."
    Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V.
    Glycobiology 17:1388-1403(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-103, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Oregon-R.
    Tissue: Head.

Entry informationi

Entry nameiMDR65_DROME
AccessioniPrimary (citable) accession number: Q00748
Secondary accession number(s): Q9VRW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 2, 2001
Last modified: November 26, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3