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Q00748

- MDR65_DROME

UniProt

Q00748 - MDR65_DROME

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Protein

Multidrug resistance protein homolog 65

Gene
Mdr65, CG10181
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi440 – 4478ATP 1 Reviewed prediction
Nucleotide bindingi1094 – 11018ATP 2 Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. efflux transmembrane transporter activity Source: FlyBase
  3. xenobiotic-transporting ATPase activity Source: FlyBase

GO - Biological processi

  1. drug transmembrane transport Source: GOC
  2. establishment of glial blood-brain barrier Source: FlyBase
  3. response to methotrexate Source: FlyBase
  4. transmembrane transport Source: RefGenome
  5. xenobiotic transport Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein homolog 65 (EC:3.6.3.44)
Alternative name(s):
P-glycoprotein 65
Gene namesi
Name:Mdr65
ORF Names:CG10181
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0004513. Mdr65.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei49 – 6921Helical; Name=1; Reviewed predictionAdd
BLAST
Topological domaini70 – 11849Extracellular Reviewed predictionAdd
BLAST
Transmembranei119 – 14729Helical; Name=2; Reviewed predictionAdd
BLAST
Topological domaini148 – 19447Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei195 – 21521Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini216 – 2238Extracellular Reviewed prediction
Transmembranei224 – 24219Helical; Name=4; Reviewed predictionAdd
BLAST
Topological domaini243 – 30260Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei303 – 32321Helical; Name=5; Reviewed predictionAdd
BLAST
Topological domaini324 – 34118Extracellular Reviewed predictionAdd
BLAST
Transmembranei342 – 36221Helical; Name=6; Reviewed predictionAdd
BLAST
Topological domaini363 – 731369Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei732 – 75322Helical; Name=7; Reviewed predictionAdd
BLAST
Topological domaini754 – 77623Extracellular Reviewed predictionAdd
BLAST
Transmembranei777 – 79822Helical; Name=8; Reviewed predictionAdd
BLAST
Topological domaini799 – 85254Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei853 – 87321Helical; Name=9; Reviewed predictionAdd
BLAST
Topological domaini874 – 8741Extracellular Reviewed prediction
Transmembranei875 – 89420Helical; Name=10; Reviewed predictionAdd
BLAST
Topological domaini895 – 95662Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei957 – 97721Helical; Name=11; Reviewed predictionAdd
BLAST
Topological domaini978 – 99316Extracellular Reviewed predictionAdd
BLAST
Transmembranei994 – 101421Helical; Name=12; Reviewed predictionAdd
BLAST
Topological domaini1015 – 1302288Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. ATP-binding cassette (ABC) transporter complex Source: FlyBase
  2. plasma membrane Source: FlyBase
  3. pleated septate junction Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13021302Multidrug resistance protein homolog 65PRO_0000093348Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi103 – 1031N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ00748.
PRIDEiQ00748.

Expressioni

Gene expression databases

BgeeiQ00748.

Interactioni

Protein-protein interaction databases

BioGridi64179. 8 interactions.
DIPiDIP-17575N.
IntActiQ00748. 2 interactions.
MINTiMINT-288870.

Structurei

3D structure databases

ProteinModelPortaliQ00748.
SMRiQ00748. Positions 35-690, 717-1300.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 369322ABC transmembrane type-1 1Add
BLAST
Domaini405 – 641237ABC transporter 1Add
BLAST
Domaini732 – 1020289ABC transmembrane type-1 2Add
BLAST
Domaini1059 – 1298240ABC transporter 2Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1132.
GeneTreeiENSGT00530000062896.
InParanoidiQ00748.
OMAiKPNFFRT.
OrthoDBiEOG7Z3F4H.
PhylomeDBiQ00748.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 3 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00748-1 [UniParc]FASTAAdd to Basket

« Hide

MERDEVSTSS SEGKSQEEAP MAEGLEPTEP IAFLKLFRFS TYGEIGWLFF     50
GFIMCCIKAL TLPAVVIIYS EFTSMLVDRA MQFGTSSNVH ALPLFGGGKT 100
LTNASREENN EALYDDSISY GILLTIASVV MFISGIFSVD VFNMVALRQV 150
TRMRIKLFSS VIRQDIGWHD LASKQNFTQS MVDDVEKIRD GISEKVGHFV 200
YLVVGFIITV AISFSYGWKL TLAVSSYIPL VILLNYYVAK FQGKLTAREQ 250
ESYAGAGNLA EEILSSIRTV VSFGGEKSEV QRYENFLVPA RKASQWKGAF 300
SGLSDAVLKS MLYLSCAGAF WYGVNLIIDD RNVENKEYTP AILMIAFFGI 350
IVGADNIART APFLESFATA RGCATNLFKV IDLTSKIDPL STDGKLLNYG 400
LRGDVEFQDV FFRYPSRPEV IVHRGLNIRI RAGQTVALVG SSGCGKSTCV 450
QLLQRFYDPV FGSVLLDDLD IRKYNIQWLR SNIAVVGQEP VLFLGTIAQN 500
ISYGKPGATQ KEIEAAATQA GAHEFITNLP ESYRSMIGER GSQLSGGQKQ 550
RIAIARALIQ NPKILLLDEA TSALDYQSEK QVQQALDLAS KGRTTIVVSH 600
RLSAIRGADK IVFIHDGKVL EEGSHDDLMA LEGAYYNMVR AGDINMPDEV 650
EKEDSIEDTK QKSLALFEKS FETSPLNFEK GQKNSVQFEE PIIKALIKDT 700
NAQSAEAPPE KPNFFRTFSR ILQLAKQEWC YLILGTISAV AVGFLYPAFA 750
VIFGEFYAAL AEKDPEDALR RTAVLSWACL GLAFLTGLVC FLQTYLFNYA 800
GIWLTTRMRA MTFNAMVNQE VGWFDDENNS VGALSARLSG EAVDIQGAIG 850
YPLSGMIQAL SNFISSVSVA MYYNWKLALL CLANCPIIVG SVILEAKMMS 900
NAVVREKQVI EEACRIATES ITNIRTVAGL RREADVIREY TEEIQRVEVL 950
IRQKLRWRGV LNSTMQASAF FAYAVALCYG GVLVSEGQLP FQDIIKVSET 1000
LLYGSMMLAQ SLAFTPAFSA ALIAGHRLFQ ILDRKPKIQS PMGTIKNTLA 1050
KQLNLFEGVR YRGIQFRYPT RPDAKILNGL DLEVLKGQTV ALVGHSGCGK 1100
STCVQLLQRY YDPDEGTIHI DHDDIQHDLT LDGVRTKLGI VSQEPTLFER 1150
SIAENIAYGD NRRSVSMVEI IAAAKSANAH SFIISLPNGY DTRMGARGTQ 1200
LSGGQKQRIA IARALVRNPK ILLLDEATSA LDLQSEQLVQ QALDTACSGR 1250
TCIVIAHRLS TVQNADVICV IQNGQVVEQG NHMQLISQGG IYAKLHKTQK 1300
DH 1302
Length:1,302
Mass (Da):143,784
Last modified:November 2, 2001 - v2
Checksum:i39A7BCABFA31924A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti369 – 3691T → S in AAA28680. 1 Publication
Sequence conflicti678 – 6781F → L in AAA28680. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59077 mRNA. Translation: AAA28680.1.
AF251287 Genomic DNA. Translation: AAF69147.1.
AF251286 Genomic DNA. Translation: AAF69146.1.
AE014296 Genomic DNA. Translation: AAF50669.1.
PIRiB41249.
RefSeqiNP_476831.1. NM_057483.4.
UniGeneiDm.4740.

Genome annotation databases

EnsemblMetazoaiFBtr0077011; FBpp0076719; FBgn0004513.
GeneIDi38726.
KEGGidme:Dmel_CG10181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59077 mRNA. Translation: AAA28680.1 .
AF251287 Genomic DNA. Translation: AAF69147.1 .
AF251286 Genomic DNA. Translation: AAF69146.1 .
AE014296 Genomic DNA. Translation: AAF50669.1 .
PIRi B41249.
RefSeqi NP_476831.1. NM_057483.4.
UniGenei Dm.4740.

3D structure databases

ProteinModelPortali Q00748.
SMRi Q00748. Positions 35-690, 717-1300.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 64179. 8 interactions.
DIPi DIP-17575N.
IntActi Q00748. 2 interactions.
MINTi MINT-288870.

Proteomic databases

PaxDbi Q00748.
PRIDEi Q00748.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0077011 ; FBpp0076719 ; FBgn0004513 .
GeneIDi 38726.
KEGGi dme:Dmel_CG10181.

Organism-specific databases

CTDi 38726.
FlyBasei FBgn0004513. Mdr65.

Phylogenomic databases

eggNOGi COG1132.
GeneTreei ENSGT00530000062896.
InParanoidi Q00748.
OMAi KPNFFRT.
OrthoDBi EOG7Z3F4H.
PhylomeDBi Q00748.

Miscellaneous databases

GenomeRNAii 38726.
NextBioi 810073.

Gene expression databases

Bgeei Q00748.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 3 hits.
PROSITEi PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of Drosophila multidrug resistance gene homologs."
    Wu C.-T., Budding M., Griffin M.S., Croop J.M.
    Mol. Cell. Biol. 11:3940-3948(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Head.
  2. "Genetics of alpha-amanitin resistance in a natural population of Drosophila melanogaster."
    Begun D.J., Whitley P.
    Heredity 85:184-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: WSIII25 and WSIII27.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. "Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster."
    Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V.
    Glycobiology 17:1388-1403(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-103, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Oregon-R.
    Tissue: Head.

Entry informationi

Entry nameiMDR65_DROME
AccessioniPrimary (citable) accession number: Q00748
Secondary accession number(s): Q9VRW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 2, 2001
Last modified: September 3, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi