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Protein

Multidrug resistance protein homolog 65

Gene

Mdr65

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi440 – 447ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1094 – 1101ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • efflux transmembrane transporter activity Source: FlyBase
  • xenobiotic-transporting ATPase activity Source: FlyBase

GO - Biological processi

  • establishment of glial blood-brain barrier Source: FlyBase
  • response to methotrexate Source: FlyBase
  • xenobiotic transport Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-159418. Recycling of bile acids and salts.
R-DME-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-DME-382556. ABC-family proteins mediated transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein homolog 65 (EC:3.6.3.44)
Alternative name(s):
P-glycoprotein 65
Gene namesi
Name:Mdr65
ORF Names:CG10181
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0004513. Mdr65.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48CytoplasmicSequence analysisAdd BLAST48
Transmembranei49 – 69Helical; Name=1PROSITE-ProRule annotationAdd BLAST21
Topological domaini70 – 118ExtracellularSequence analysisAdd BLAST49
Transmembranei119 – 147Helical; Name=2PROSITE-ProRule annotationAdd BLAST29
Topological domaini148 – 194CytoplasmicSequence analysisAdd BLAST47
Transmembranei195 – 215Helical; Name=3PROSITE-ProRule annotationAdd BLAST21
Topological domaini216 – 223ExtracellularSequence analysis8
Transmembranei224 – 242Helical; Name=4PROSITE-ProRule annotationAdd BLAST19
Topological domaini243 – 302CytoplasmicSequence analysisAdd BLAST60
Transmembranei303 – 323Helical; Name=5PROSITE-ProRule annotationAdd BLAST21
Topological domaini324 – 341ExtracellularSequence analysisAdd BLAST18
Transmembranei342 – 362Helical; Name=6PROSITE-ProRule annotationAdd BLAST21
Topological domaini363 – 731CytoplasmicSequence analysisAdd BLAST369
Transmembranei732 – 753Helical; Name=7PROSITE-ProRule annotationAdd BLAST22
Topological domaini754 – 776ExtracellularSequence analysisAdd BLAST23
Transmembranei777 – 798Helical; Name=8PROSITE-ProRule annotationAdd BLAST22
Topological domaini799 – 852CytoplasmicSequence analysisAdd BLAST54
Transmembranei853 – 873Helical; Name=9PROSITE-ProRule annotationAdd BLAST21
Topological domaini874ExtracellularSequence analysis1
Transmembranei875 – 894Helical; Name=10PROSITE-ProRule annotationAdd BLAST20
Topological domaini895 – 956CytoplasmicSequence analysisAdd BLAST62
Transmembranei957 – 977Helical; Name=11PROSITE-ProRule annotationAdd BLAST21
Topological domaini978 – 993ExtracellularSequence analysisAdd BLAST16
Transmembranei994 – 1014Helical; Name=12PROSITE-ProRule annotationAdd BLAST21
Topological domaini1015 – 1302CytoplasmicSequence analysisAdd BLAST288

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: FlyBase
  • plasma membrane Source: FlyBase
  • pleated septate junction Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933481 – 1302Multidrug resistance protein homolog 65Add BLAST1302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi103N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ00748.
PRIDEiQ00748.

Expressioni

Gene expression databases

BgeeiFBgn0004513.
GenevisibleiQ00748. DM.

Interactioni

Protein-protein interaction databases

BioGridi64179. 8 interactors.
DIPiDIP-17575N.
IntActiQ00748. 2 interactors.
MINTiMINT-288870.
STRINGi7227.FBpp0076719.

Structurei

3D structure databases

ProteinModelPortaliQ00748.
SMRiQ00748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 369ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST322
Domaini405 – 641ABC transporter 1PROSITE-ProRule annotationAdd BLAST237
Domaini732 – 1020ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST289
Domaini1059 – 1298ABC transporter 2PROSITE-ProRule annotationAdd BLAST240

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00530000062896.
InParanoidiQ00748.
OMAiFLYPAFA.
OrthoDBiEOG091G0HVA.
PhylomeDBiQ00748.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 3 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERDEVSTSS SEGKSQEEAP MAEGLEPTEP IAFLKLFRFS TYGEIGWLFF
60 70 80 90 100
GFIMCCIKAL TLPAVVIIYS EFTSMLVDRA MQFGTSSNVH ALPLFGGGKT
110 120 130 140 150
LTNASREENN EALYDDSISY GILLTIASVV MFISGIFSVD VFNMVALRQV
160 170 180 190 200
TRMRIKLFSS VIRQDIGWHD LASKQNFTQS MVDDVEKIRD GISEKVGHFV
210 220 230 240 250
YLVVGFIITV AISFSYGWKL TLAVSSYIPL VILLNYYVAK FQGKLTAREQ
260 270 280 290 300
ESYAGAGNLA EEILSSIRTV VSFGGEKSEV QRYENFLVPA RKASQWKGAF
310 320 330 340 350
SGLSDAVLKS MLYLSCAGAF WYGVNLIIDD RNVENKEYTP AILMIAFFGI
360 370 380 390 400
IVGADNIART APFLESFATA RGCATNLFKV IDLTSKIDPL STDGKLLNYG
410 420 430 440 450
LRGDVEFQDV FFRYPSRPEV IVHRGLNIRI RAGQTVALVG SSGCGKSTCV
460 470 480 490 500
QLLQRFYDPV FGSVLLDDLD IRKYNIQWLR SNIAVVGQEP VLFLGTIAQN
510 520 530 540 550
ISYGKPGATQ KEIEAAATQA GAHEFITNLP ESYRSMIGER GSQLSGGQKQ
560 570 580 590 600
RIAIARALIQ NPKILLLDEA TSALDYQSEK QVQQALDLAS KGRTTIVVSH
610 620 630 640 650
RLSAIRGADK IVFIHDGKVL EEGSHDDLMA LEGAYYNMVR AGDINMPDEV
660 670 680 690 700
EKEDSIEDTK QKSLALFEKS FETSPLNFEK GQKNSVQFEE PIIKALIKDT
710 720 730 740 750
NAQSAEAPPE KPNFFRTFSR ILQLAKQEWC YLILGTISAV AVGFLYPAFA
760 770 780 790 800
VIFGEFYAAL AEKDPEDALR RTAVLSWACL GLAFLTGLVC FLQTYLFNYA
810 820 830 840 850
GIWLTTRMRA MTFNAMVNQE VGWFDDENNS VGALSARLSG EAVDIQGAIG
860 870 880 890 900
YPLSGMIQAL SNFISSVSVA MYYNWKLALL CLANCPIIVG SVILEAKMMS
910 920 930 940 950
NAVVREKQVI EEACRIATES ITNIRTVAGL RREADVIREY TEEIQRVEVL
960 970 980 990 1000
IRQKLRWRGV LNSTMQASAF FAYAVALCYG GVLVSEGQLP FQDIIKVSET
1010 1020 1030 1040 1050
LLYGSMMLAQ SLAFTPAFSA ALIAGHRLFQ ILDRKPKIQS PMGTIKNTLA
1060 1070 1080 1090 1100
KQLNLFEGVR YRGIQFRYPT RPDAKILNGL DLEVLKGQTV ALVGHSGCGK
1110 1120 1130 1140 1150
STCVQLLQRY YDPDEGTIHI DHDDIQHDLT LDGVRTKLGI VSQEPTLFER
1160 1170 1180 1190 1200
SIAENIAYGD NRRSVSMVEI IAAAKSANAH SFIISLPNGY DTRMGARGTQ
1210 1220 1230 1240 1250
LSGGQKQRIA IARALVRNPK ILLLDEATSA LDLQSEQLVQ QALDTACSGR
1260 1270 1280 1290 1300
TCIVIAHRLS TVQNADVICV IQNGQVVEQG NHMQLISQGG IYAKLHKTQK

DH
Length:1,302
Mass (Da):143,784
Last modified:November 2, 2001 - v2
Checksum:i39A7BCABFA31924A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti369T → S in AAA28680 (PubMed:2072901).Curated1
Sequence conflicti678F → L in AAA28680 (PubMed:2072901).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59077 mRNA. Translation: AAA28680.1.
AF251287 Genomic DNA. Translation: AAF69147.1.
AF251286 Genomic DNA. Translation: AAF69146.1.
AE014296 Genomic DNA. Translation: AAF50669.1.
PIRiB41249.
RefSeqiNP_476831.1. NM_057483.4.
UniGeneiDm.4740.

Genome annotation databases

EnsemblMetazoaiFBtr0077011; FBpp0076719; FBgn0004513.
GeneIDi38726.
KEGGidme:Dmel_CG10181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59077 mRNA. Translation: AAA28680.1.
AF251287 Genomic DNA. Translation: AAF69147.1.
AF251286 Genomic DNA. Translation: AAF69146.1.
AE014296 Genomic DNA. Translation: AAF50669.1.
PIRiB41249.
RefSeqiNP_476831.1. NM_057483.4.
UniGeneiDm.4740.

3D structure databases

ProteinModelPortaliQ00748.
SMRiQ00748.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64179. 8 interactors.
DIPiDIP-17575N.
IntActiQ00748. 2 interactors.
MINTiMINT-288870.
STRINGi7227.FBpp0076719.

Proteomic databases

PaxDbiQ00748.
PRIDEiQ00748.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077011; FBpp0076719; FBgn0004513.
GeneIDi38726.
KEGGidme:Dmel_CG10181.

Organism-specific databases

CTDi38726.
FlyBaseiFBgn0004513. Mdr65.

Phylogenomic databases

eggNOGiKOG0055. Eukaryota.
COG1132. LUCA.
GeneTreeiENSGT00530000062896.
InParanoidiQ00748.
OMAiFLYPAFA.
OrthoDBiEOG091G0HVA.
PhylomeDBiQ00748.

Enzyme and pathway databases

ReactomeiR-DME-159418. Recycling of bile acids and salts.
R-DME-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-DME-382556. ABC-family proteins mediated transport.

Miscellaneous databases

GenomeRNAii38726.
PROiQ00748.

Gene expression databases

BgeeiFBgn0004513.
GenevisibleiQ00748. DM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 3 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDR65_DROME
AccessioniPrimary (citable) accession number: Q00748
Secondary accession number(s): Q9VRW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 2, 2001
Last modified: November 30, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.