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Q00748 (MDR65_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multidrug resistance protein homolog 65
EC=3.6.3.44
Alternative name(s):
P-glycoprotein 65
Gene names
Name:Mdr65
ORF Names:CG10181
OrganismDrosophila melanogaster (Fruit fly)
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length1302 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13021302Multidrug resistance protein homolog 65
PRO_0000093348

Regions

Topological domain1 – 4848Cytoplasmic Potential
Transmembrane49 – 6921Helical; Name=1; Potential
Topological domain70 – 11849Extracellular Potential
Transmembrane119 – 14729Helical; Name=2; Potential
Topological domain148 – 19447Cytoplasmic Potential
Transmembrane195 – 21521Helical; Name=3; Potential
Topological domain216 – 2238Extracellular Potential
Transmembrane224 – 24219Helical; Name=4; Potential
Topological domain243 – 30260Cytoplasmic Potential
Transmembrane303 – 32321Helical; Name=5; Potential
Topological domain324 – 34118Extracellular Potential
Transmembrane342 – 36221Helical; Name=6; Potential
Topological domain363 – 731369Cytoplasmic Potential
Transmembrane732 – 75322Helical; Name=7; Potential
Topological domain754 – 77623Extracellular Potential
Transmembrane777 – 79822Helical; Name=8; Potential
Topological domain799 – 85254Cytoplasmic Potential
Transmembrane853 – 87321Helical; Name=9; Potential
Topological domain8741Extracellular Potential
Transmembrane875 – 89420Helical; Name=10; Potential
Topological domain895 – 95662Cytoplasmic Potential
Transmembrane957 – 97721Helical; Name=11; Potential
Topological domain978 – 99316Extracellular Potential
Transmembrane994 – 101421Helical; Name=12; Potential
Topological domain1015 – 1302288Cytoplasmic Potential
Domain48 – 369322ABC transmembrane type-1 1
Domain405 – 641237ABC transporter 1
Domain732 – 1020289ABC transmembrane type-1 2
Domain1059 – 1298240ABC transporter 2
Nucleotide binding440 – 4478ATP 1 Potential
Nucleotide binding1094 – 11018ATP 2 Potential

Amino acid modifications

Glycosylation1031N-linked (GlcNAc...) Ref.5

Experimental info

Sequence conflict3691T → S in AAA28680. Ref.1
Sequence conflict6781F → L in AAA28680. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q00748 [UniParc].

Last modified November 2, 2001. Version 2.
Checksum: 39A7BCABFA31924A

FASTA1,302143,784
        10         20         30         40         50         60 
MERDEVSTSS SEGKSQEEAP MAEGLEPTEP IAFLKLFRFS TYGEIGWLFF GFIMCCIKAL 

        70         80         90        100        110        120 
TLPAVVIIYS EFTSMLVDRA MQFGTSSNVH ALPLFGGGKT LTNASREENN EALYDDSISY 

       130        140        150        160        170        180 
GILLTIASVV MFISGIFSVD VFNMVALRQV TRMRIKLFSS VIRQDIGWHD LASKQNFTQS 

       190        200        210        220        230        240 
MVDDVEKIRD GISEKVGHFV YLVVGFIITV AISFSYGWKL TLAVSSYIPL VILLNYYVAK 

       250        260        270        280        290        300 
FQGKLTAREQ ESYAGAGNLA EEILSSIRTV VSFGGEKSEV QRYENFLVPA RKASQWKGAF 

       310        320        330        340        350        360 
SGLSDAVLKS MLYLSCAGAF WYGVNLIIDD RNVENKEYTP AILMIAFFGI IVGADNIART 

       370        380        390        400        410        420 
APFLESFATA RGCATNLFKV IDLTSKIDPL STDGKLLNYG LRGDVEFQDV FFRYPSRPEV 

       430        440        450        460        470        480 
IVHRGLNIRI RAGQTVALVG SSGCGKSTCV QLLQRFYDPV FGSVLLDDLD IRKYNIQWLR 

       490        500        510        520        530        540 
SNIAVVGQEP VLFLGTIAQN ISYGKPGATQ KEIEAAATQA GAHEFITNLP ESYRSMIGER 

       550        560        570        580        590        600 
GSQLSGGQKQ RIAIARALIQ NPKILLLDEA TSALDYQSEK QVQQALDLAS KGRTTIVVSH 

       610        620        630        640        650        660 
RLSAIRGADK IVFIHDGKVL EEGSHDDLMA LEGAYYNMVR AGDINMPDEV EKEDSIEDTK 

       670        680        690        700        710        720 
QKSLALFEKS FETSPLNFEK GQKNSVQFEE PIIKALIKDT NAQSAEAPPE KPNFFRTFSR 

       730        740        750        760        770        780 
ILQLAKQEWC YLILGTISAV AVGFLYPAFA VIFGEFYAAL AEKDPEDALR RTAVLSWACL 

       790        800        810        820        830        840 
GLAFLTGLVC FLQTYLFNYA GIWLTTRMRA MTFNAMVNQE VGWFDDENNS VGALSARLSG 

       850        860        870        880        890        900 
EAVDIQGAIG YPLSGMIQAL SNFISSVSVA MYYNWKLALL CLANCPIIVG SVILEAKMMS 

       910        920        930        940        950        960 
NAVVREKQVI EEACRIATES ITNIRTVAGL RREADVIREY TEEIQRVEVL IRQKLRWRGV 

       970        980        990       1000       1010       1020 
LNSTMQASAF FAYAVALCYG GVLVSEGQLP FQDIIKVSET LLYGSMMLAQ SLAFTPAFSA 

      1030       1040       1050       1060       1070       1080 
ALIAGHRLFQ ILDRKPKIQS PMGTIKNTLA KQLNLFEGVR YRGIQFRYPT RPDAKILNGL 

      1090       1100       1110       1120       1130       1140 
DLEVLKGQTV ALVGHSGCGK STCVQLLQRY YDPDEGTIHI DHDDIQHDLT LDGVRTKLGI 

      1150       1160       1170       1180       1190       1200 
VSQEPTLFER SIAENIAYGD NRRSVSMVEI IAAAKSANAH SFIISLPNGY DTRMGARGTQ 

      1210       1220       1230       1240       1250       1260 
LSGGQKQRIA IARALVRNPK ILLLDEATSA LDLQSEQLVQ QALDTACSGR TCIVIAHRLS 

      1270       1280       1290       1300 
TVQNADVICV IQNGQVVEQG NHMQLISQGG IYAKLHKTQK DH

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of Drosophila multidrug resistance gene homologs."
Wu C.-T., Budding M., Griffin M.S., Croop J.M.
Mol. Cell. Biol. 11:3940-3948(1991) [PubMed: 2072901] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Head.
[2]"Genetics of alpha-amanitin resistance in a natural population of Drosophila melanogaster."
Begun D.J., Whitley P.
Heredity 85:184-190(2000) [PubMed: 11012721] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: WSIII25 and WSIII27.
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[5]"Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster."
Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V.
Glycobiology 17:1388-1403(2007) [PubMed: 17893096] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-103, MASS SPECTROMETRY.
Strain: Oregon-R.
Tissue: Head.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M59077 mRNA. Translation: AAA28680.1.
AF251287 Genomic DNA. Translation: AAF69147.1.
AF251286 Genomic DNA. Translation: AAF69146.1.
AE014296 Genomic DNA. Translation: AAF50669.1.
PIRB41249.
RefSeqNP_476831.1. NM_057483.3.
UniGeneDm.4740.

3D structure databases

ProteinModelPortalQ00748.
SMRQ00748. Positions 31-643, 718-1301.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-17575N.
IntActQ00748. 2 interactions.
MINTMINT-288870.
STRINGQ00748.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0077011; FBpp0076719; FBgn0004513.
GeneID38726.
KEGGdme:Dmel_CG10181.
NMPDRfig|7227.3.peg.8529.

Organism-specific databases

CTD38726.
FlyBaseFBgn0004513. Mdr65.

Phylogenomic databases

eggNOGinNOG05231.
InParanoidQ00748.
OrthoDBEOG4N2Z3R.
PhylomeDBQ00748.

Gene expression databases

ArrayExpressQ00748.
BgeeQ00748.
GermOnlineCG10181. Drosophila melanogaster.

Family and domain databases

InterProIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR001140. ABC_transptr_TM_dom.
IPR011527. ABC_transptrTM_dom_typ1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF90123. ABC_TM_1. 2 hits.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio810073.

Entry information

Entry nameMDR65_DROME
AccessionPrimary (citable) accession number: Q00748
Secondary accession number(s): Q9VRW3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 2, 2001
Last modified: January 25, 2012
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents