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Q00748

- MDR65_DROME

UniProt

Q00748 - MDR65_DROME

Protein

Multidrug resistance protein homolog 65

Gene

Mdr65

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (02 Nov 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi440 – 4478ATP 1PROSITE-ProRule annotation
    Nucleotide bindingi1094 – 11018ATP 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. efflux transmembrane transporter activity Source: FlyBase
    3. xenobiotic-transporting ATPase activity Source: FlyBase

    GO - Biological processi

    1. drug transmembrane transport Source: GOC
    2. establishment of glial blood-brain barrier Source: FlyBase
    3. response to methotrexate Source: FlyBase
    4. transmembrane transport Source: RefGenome
    5. xenobiotic transport Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multidrug resistance protein homolog 65 (EC:3.6.3.44)
    Alternative name(s):
    P-glycoprotein 65
    Gene namesi
    Name:Mdr65
    ORF Names:CG10181
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3L

    Organism-specific databases

    FlyBaseiFBgn0004513. Mdr65.

    Subcellular locationi

    GO - Cellular componenti

    1. ATP-binding cassette (ABC) transporter complex Source: FlyBase
    2. plasma membrane Source: FlyBase
    3. pleated septate junction Source: FlyBase

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13021302Multidrug resistance protein homolog 65PRO_0000093348Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi103 – 1031N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ00748.
    PRIDEiQ00748.

    Expressioni

    Gene expression databases

    BgeeiQ00748.

    Interactioni

    Protein-protein interaction databases

    BioGridi64179. 8 interactions.
    DIPiDIP-17575N.
    IntActiQ00748. 2 interactions.
    MINTiMINT-288870.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00748.
    SMRiQ00748. Positions 35-690, 717-1300.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4848CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini70 – 11849ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini148 – 19447CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini216 – 2238ExtracellularSequence Analysis
    Topological domaini243 – 30260CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini324 – 34118ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini363 – 731369CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini754 – 77623ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini799 – 85254CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini874 – 8741ExtracellularSequence Analysis
    Topological domaini895 – 95662CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini978 – 99316ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1015 – 1302288CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei49 – 6921Helical; Name=1PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei119 – 14729Helical; Name=2PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei195 – 21521Helical; Name=3PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei224 – 24219Helical; Name=4PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei303 – 32321Helical; Name=5PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei342 – 36221Helical; Name=6PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei732 – 75322Helical; Name=7PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei777 – 79822Helical; Name=8PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei853 – 87321Helical; Name=9PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei875 – 89420Helical; Name=10PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei957 – 97721Helical; Name=11PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei994 – 101421Helical; Name=12PROSITE-ProRule annotationAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini48 – 369322ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini405 – 641237ABC transporter 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini732 – 1020289ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1059 – 1298240ABC transporter 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
    Contains 2 ABC transporter domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1132.
    GeneTreeiENSGT00530000062896.
    InParanoidiQ00748.
    OMAiKPNFFRT.
    OrthoDBiEOG7Z3F4H.
    PhylomeDBiQ00748.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00664. ABC_membrane. 2 hits.
    PF00005. ABC_tran. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF90123. SSF90123. 3 hits.
    PROSITEiPS50929. ABC_TM1F. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 2 hits.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00748-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERDEVSTSS SEGKSQEEAP MAEGLEPTEP IAFLKLFRFS TYGEIGWLFF     50
    GFIMCCIKAL TLPAVVIIYS EFTSMLVDRA MQFGTSSNVH ALPLFGGGKT 100
    LTNASREENN EALYDDSISY GILLTIASVV MFISGIFSVD VFNMVALRQV 150
    TRMRIKLFSS VIRQDIGWHD LASKQNFTQS MVDDVEKIRD GISEKVGHFV 200
    YLVVGFIITV AISFSYGWKL TLAVSSYIPL VILLNYYVAK FQGKLTAREQ 250
    ESYAGAGNLA EEILSSIRTV VSFGGEKSEV QRYENFLVPA RKASQWKGAF 300
    SGLSDAVLKS MLYLSCAGAF WYGVNLIIDD RNVENKEYTP AILMIAFFGI 350
    IVGADNIART APFLESFATA RGCATNLFKV IDLTSKIDPL STDGKLLNYG 400
    LRGDVEFQDV FFRYPSRPEV IVHRGLNIRI RAGQTVALVG SSGCGKSTCV 450
    QLLQRFYDPV FGSVLLDDLD IRKYNIQWLR SNIAVVGQEP VLFLGTIAQN 500
    ISYGKPGATQ KEIEAAATQA GAHEFITNLP ESYRSMIGER GSQLSGGQKQ 550
    RIAIARALIQ NPKILLLDEA TSALDYQSEK QVQQALDLAS KGRTTIVVSH 600
    RLSAIRGADK IVFIHDGKVL EEGSHDDLMA LEGAYYNMVR AGDINMPDEV 650
    EKEDSIEDTK QKSLALFEKS FETSPLNFEK GQKNSVQFEE PIIKALIKDT 700
    NAQSAEAPPE KPNFFRTFSR ILQLAKQEWC YLILGTISAV AVGFLYPAFA 750
    VIFGEFYAAL AEKDPEDALR RTAVLSWACL GLAFLTGLVC FLQTYLFNYA 800
    GIWLTTRMRA MTFNAMVNQE VGWFDDENNS VGALSARLSG EAVDIQGAIG 850
    YPLSGMIQAL SNFISSVSVA MYYNWKLALL CLANCPIIVG SVILEAKMMS 900
    NAVVREKQVI EEACRIATES ITNIRTVAGL RREADVIREY TEEIQRVEVL 950
    IRQKLRWRGV LNSTMQASAF FAYAVALCYG GVLVSEGQLP FQDIIKVSET 1000
    LLYGSMMLAQ SLAFTPAFSA ALIAGHRLFQ ILDRKPKIQS PMGTIKNTLA 1050
    KQLNLFEGVR YRGIQFRYPT RPDAKILNGL DLEVLKGQTV ALVGHSGCGK 1100
    STCVQLLQRY YDPDEGTIHI DHDDIQHDLT LDGVRTKLGI VSQEPTLFER 1150
    SIAENIAYGD NRRSVSMVEI IAAAKSANAH SFIISLPNGY DTRMGARGTQ 1200
    LSGGQKQRIA IARALVRNPK ILLLDEATSA LDLQSEQLVQ QALDTACSGR 1250
    TCIVIAHRLS TVQNADVICV IQNGQVVEQG NHMQLISQGG IYAKLHKTQK 1300
    DH 1302
    Length:1,302
    Mass (Da):143,784
    Last modified:November 2, 2001 - v2
    Checksum:i39A7BCABFA31924A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti369 – 3691T → S in AAA28680. (PubMed:2072901)Curated
    Sequence conflicti678 – 6781F → L in AAA28680. (PubMed:2072901)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59077 mRNA. Translation: AAA28680.1.
    AF251287 Genomic DNA. Translation: AAF69147.1.
    AF251286 Genomic DNA. Translation: AAF69146.1.
    AE014296 Genomic DNA. Translation: AAF50669.1.
    PIRiB41249.
    RefSeqiNP_476831.1. NM_057483.4.
    UniGeneiDm.4740.

    Genome annotation databases

    EnsemblMetazoaiFBtr0077011; FBpp0076719; FBgn0004513.
    GeneIDi38726.
    KEGGidme:Dmel_CG10181.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59077 mRNA. Translation: AAA28680.1 .
    AF251287 Genomic DNA. Translation: AAF69147.1 .
    AF251286 Genomic DNA. Translation: AAF69146.1 .
    AE014296 Genomic DNA. Translation: AAF50669.1 .
    PIRi B41249.
    RefSeqi NP_476831.1. NM_057483.4.
    UniGenei Dm.4740.

    3D structure databases

    ProteinModelPortali Q00748.
    SMRi Q00748. Positions 35-690, 717-1300.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 64179. 8 interactions.
    DIPi DIP-17575N.
    IntActi Q00748. 2 interactions.
    MINTi MINT-288870.

    Proteomic databases

    PaxDbi Q00748.
    PRIDEi Q00748.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0077011 ; FBpp0076719 ; FBgn0004513 .
    GeneIDi 38726.
    KEGGi dme:Dmel_CG10181.

    Organism-specific databases

    CTDi 38726.
    FlyBasei FBgn0004513. Mdr65.

    Phylogenomic databases

    eggNOGi COG1132.
    GeneTreei ENSGT00530000062896.
    InParanoidi Q00748.
    OMAi KPNFFRT.
    OrthoDBi EOG7Z3F4H.
    PhylomeDBi Q00748.

    Miscellaneous databases

    GenomeRNAii 38726.
    NextBioi 810073.

    Gene expression databases

    Bgeei Q00748.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00664. ABC_membrane. 2 hits.
    PF00005. ABC_tran. 2 hits.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF90123. SSF90123. 3 hits.
    PROSITEi PS50929. ABC_TM1F. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 2 hits.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of Drosophila multidrug resistance gene homologs."
      Wu C.-T., Budding M., Griffin M.S., Croop J.M.
      Mol. Cell. Biol. 11:3940-3948(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Head.
    2. "Genetics of alpha-amanitin resistance in a natural population of Drosophila melanogaster."
      Begun D.J., Whitley P.
      Heredity 85:184-190(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: WSIII25 and WSIII27.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. "Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster."
      Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V.
      Glycobiology 17:1388-1403(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-103, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Oregon-R.
      Tissue: Head.

    Entry informationi

    Entry nameiMDR65_DROME
    AccessioniPrimary (citable) accession number: Q00748
    Secondary accession number(s): Q9VRW3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: November 2, 2001
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3