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Q00722 (PLCB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C-beta-2
Phospholipase C-beta-2
Short name=PLC-beta-2
Gene names
Name:PLCB2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1185 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactor

Binds 1 calcium ion per subunit.

Subunit structure

Interacts with RAC1.

Miscellaneous

The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16.

Sequence similarities

Contains 1 C2 domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q00722-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q00722-2)

The sequence of this isoform differs from the canonical sequence as follows:
     492-495: Missing.
Isoform 3 (identifier: Q00722-3)

The sequence of this isoform differs from the canonical sequence as follows:
     868-882: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 118511851-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
PRO_0000088489

Regions

Domain312 – 463152PI-PLC X-box
Domain546 – 662117PI-PLC Y-box
Domain669 – 76799C2
Coiled coil988 – 1147160 Potential
Compositional bias500 – 54546Glu-rich

Sites

Active site3271 By similarity
Active site3741 By similarity
Metal binding3281Calcium
Metal binding3571Calcium
Metal binding3591Calcium
Metal binding4081Calcium

Natural variations

Alternative sequence492 – 4954Missing in isoform 2.
VSP_035770
Alternative sequence868 – 88215Missing in isoform 3.
VSP_054490
Natural variant3241N → I.
Corresponds to variant rs45628633 [ dbSNP | Ensembl ].
VAR_047509
Natural variant5981R → H.
Corresponds to variant rs8025153 [ dbSNP | Ensembl ].
VAR_047510
Natural variant6641P → L.
Corresponds to variant rs9972332 [ dbSNP | Ensembl ].
VAR_047511
Natural variant7121G → R.
Corresponds to variant rs28395835 [ dbSNP | Ensembl ].
VAR_047512
Natural variant10951E → G. Ref.2
Corresponds to variant rs936212 [ dbSNP | Ensembl ].
VAR_047513

Experimental info

Mutagenesis521Q → A: Strongly reduces interaction with RAC1. Ref.5
Sequence conflict1191K → R in BAF84346. Ref.2
Sequence conflict10601K → R in BAF84346. Ref.2

Secondary structure

..................................................................................................................... 1185
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 25, 2008. Version 2.
Checksum: A0397309D943672A

FASTA1,185134,024
        10         20         30         40         50         60 
MSLLNPVLLP PKVKAYLSQG ERFIKWDDET TVASPVILRV DPKGYYLYWT YQSKEMEFLD 

        70         80         90        100        110        120 
ITSIRDTRFG KFAKMPKSQK LRDVFNMDFP DNSFLLKTLT VVSGPDMVDL TFHNFVSYKE 

       130        140        150        160        170        180 
NVGKAWAEDV LALVKHPLTA NASRSTFLDK ILVKLKMQLN SEGKIPVKNF FQMFPADRKR 

       190        200        210        220        230        240 
VEAALSACHL PKGKNDAINP EDFPEPVYKS FLMSLCPRPE IDEIFTSYHA KAKPYMTKEH 

       250        260        270        280        290        300 
LTKFINQKQR DSRLNSLLFP PARPDQVQGL IDKYEPSGIN AQRGQLSPEG MVWFLCGPEN 

       310        320        330        340        350        360 
SVLAQDKLLL HHDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL SGCRCVELDC 

       370        380        390        400        410        420 
WKGKPPDEEP IITHGFTMTT DIFFKEAIEA IAESAFKTSP YPIILSFENH VDSPRQQAKM 

       430        440        450        460        470        480 
AEYCRTIFGD MLLTEPLEKF PLKPGVPLPS PEDLRGKILI KNKKNQFSGP TSSSKDTGGE 

       490        500        510        520        530        540 
AEGSSPPSAP AGEGTVWAGE EGTELEEEEV EEEEEEESGN LDEEEIKKMQ SDEGTAGLEV 

       550        560        570        580        590        600 
TAYEEMSSLV NYIQPTKFVS FEFSAQKNRS YVISSFTELK AYDLLSKASV QFVDYNKRQM 

       610        620        630        640        650        660 
SRIYPKGTRM DSSNYMPQMF WNAGCQMVAL NFQTMDLPMQ QNMAVFEFNG QSGYLLKHEF 

       670        680        690        700        710        720 
MRRPDKQFNP FSVDRIDVVV ATTLSITVIS GQFLSERSVR TYVEVELFGL PGDPKRRYRT 

       730        740        750        760        770        780 
KLSPSTNSIN PVWKEEPFVF EKILMPELAS LRVAVMEEGN KFLGHRIIPI NALNSGYHHL 

       790        800        810        820        830        840 
CLHSESNMPL TMPALFIFLE MKDYIPGAWA DLTVALANPI KFFSAHDTKS VKLKEAMGGL 

       850        860        870        880        890        900 
PEKPFPLASP VASQVNGALA PTSNGSPAAR AGAREEAMKE AAEPRTASLE ELRELKGVVK 

       910        920        930        940        950        960 
LQRRHEKELR ELERRGARRW EELLQRGAAQ LAELGPPGVG GVGACKLGPG KGSRKKRSLP 

       970        980        990       1000       1010       1020 
REESAGAAPG EGPEGVDGRV RELKDRLELE LLRQGEEQYE CVLKRKEQHV AEQISKMMEL 

      1030       1040       1050       1060       1070       1080 
AREKQAAELK ALKETSENDT KEMKKKLETK RLERIQGMTK VTTDKMAQER LKREINNSHI 

      1090       1100       1110       1120       1130       1140 
QEVVQVIKQM TENLERHQEK LEEKQAACLE QIREMEKQFQ KEALAEYEAR MKGLEAEVKE 

      1150       1160       1170       1180 
SVRACLRTCF PSEAKDKPER ACECPPELCE QDPLIAKADA QESRL 

« Hide

Isoform 2 [UniParc].

Checksum: 7A6889F204D17FA4
Show »

FASTA1,181133,680
Isoform 3 [UniParc].

Checksum: BC9DC96247E5762D
Show »

FASTA1,170132,511

References

« Hide 'large scale' references
[1]"Cloning, sequencing, expression, and Gq-independent activation of phospholipase C-beta 2."
Park D., Jhon D.-Y., Kriz R., Knopf J., Rhee S.G.
J. Biol. Chem. 267:16048-16055(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLY-1095.
Tissue: Placenta.
[3]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Testis.
[5]"Crystal structure of Rac1 bound to its effector phospholipase C-beta2."
Jezyk M.R., Snyder J.T., Gershberg S., Worthylake D.K., Harden T.K., Sondek J.
Nat. Struct. Mol. Biol. 13:1135-1140(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-803 (ISOFORM 2) IN COMPLEX WITH RAC1, MUTAGENESIS OF GLN-52.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95678 mRNA. Translation: AAA36453.1.
AK291657 mRNA. Translation: BAF84346.1.
AC020658 Genomic DNA. No translation available.
BC136467 mRNA. Translation: AAI36468.1.
CCDSCCDS42020.1. [Q00722-1]
CCDS61592.1. [Q00722-2]
PIRA43346.
RefSeqNP_001271226.1. NM_001284297.1. [Q00722-2]
NP_001271227.1. NM_001284298.1. [Q00722-3]
NP_004564.2. NM_004573.2. [Q00722-1]
UniGeneHs.355888.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FJUX-ray2.20B1-803[»]
2ZKMX-ray1.62X1-799[»]
ProteinModelPortalQ00722.
SMRQ00722. Positions 11-803, 883-1151.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111346. 12 interactions.
DIPDIP-29259N.
MINTMINT-1726830.
STRING9606.ENSP00000260402.

PTM databases

PhosphoSiteQ00722.

Polymorphism databases

DMDM215273902.

Proteomic databases

MaxQBQ00722.
PaxDbQ00722.
PRIDEQ00722.

Protocols and materials databases

DNASU5330.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000260402; ENSP00000260402; ENSG00000137841. [Q00722-1]
ENST00000456256; ENSP00000411991; ENSG00000137841.
ENST00000557821; ENSP00000453975; ENSG00000137841. [Q00722-2]
GeneID5330.
KEGGhsa:5330.
UCSCuc001zld.3. human. [Q00722-1]
uc010bbo.3. human. [Q00722-2]

Organism-specific databases

CTD5330.
GeneCardsGC15M040580.
H-InvDBHIX0012125.
HGNCHGNC:9055. PLCB2.
HPACAB009443.
MIM604114. gene.
neXtProtNX_Q00722.
PharmGKBPA33385.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG149692.
HOGENOMHOG000232046.
HOVERGENHBG053609.
InParanoidQ00722.
KOK05858.
OMAFIKWDDE.
OrthoDBEOG7WDN1N.
PhylomeDBQ00722.
TreeFamTF313216.

Enzyme and pathway databases

BioCycMetaCyc:HS06408-MONOMER.
BRENDA3.1.4.11. 2681.
ReactomeREACT_111102. Signal Transduction.
REACT_111217. Metabolism.
REACT_13685. Neuronal System.
SignaLinkQ00722.

Gene expression databases

ArrayExpressQ00722.
BgeeQ00722.
CleanExHS_PLCB2.
GenevestigatorQ00722.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028403. PLC-beta2.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF10. PTHR10336:SF10. 1 hit.
PfamPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFPIRSF000956. PLC-beta. 1 hit.
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPLCB2. human.
EvolutionaryTraceQ00722.
GeneWikiPLCB2.
GenomeRNAi5330.
NextBio20640.
PROQ00722.
SOURCESearch...

Entry information

Entry namePLCB2_HUMAN
AccessionPrimary (citable) accession number: Q00722
Secondary accession number(s): A8K6J2, B9EGH5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 25, 2008
Last modified: July 9, 2014
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM