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Q00721 (NSP3_ROTS4) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 3

Short name=NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name=NS34
OrganismRotavirus A (strain SA11-4F G3-P6[1]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Simian Agent 11 (strain 4F))
Taxonomic identifier36436 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.

Subunit structure

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Sequence similarities

Belongs to the rotavirus A NSP3 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ZC3H7BQ9UGR26EBI-1263962,EBI-948845From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Non-structural protein 3
PRO_0000149542

Regions

Region4 – 149146RNA-binding By similarity
Region150 – 20657Dimerization By similarity
Region170 – 23465Interaction with ZC3H7B By similarity
Region208 – 315108Interaction with EIF4G1 By similarity
Coiled coil166 – 23772 Potential

Sequences

Sequence LengthMass (Da)Tools
Q00721 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 9E5D39FDE6FCE73B

FASTA31536,451
        10         20         30         40         50         60 
MLKMESTQQM AVSIINSSFE AAVVAATSAL ENMGIEYDYQ DIYSRVKNKF DFVMDDSGVK 

        70         80         90        100        110        120 
NNLIGKAITI DQALNNKFGS AIRNRNWLAD TSRPAKLDED VNKLRMMLSS KGIDQKMRVL 

       130        140        150        160        170        180 
NACFSVKRIP GKSSSIIKCT KLMRDKLERG EVEVDDSFVD EKMEVDTIDW KSRYEQLEQR 

       190        200        210        220        230        240 
FESLKSRVNE KYNNWVLKAR KMNENMHSLQ NVISQQQAHI AELQVYNNKL ERDLQNKIGS 

       250        260        270        280        290        300 
LTSSIEWYLR SMELDPEIKA DIEQQINSID AINPLHAFDD LESVIRNLIS DYDKLFLMFK 

       310 
GLIQRCNYQY SFGCE 

« Hide

References

[1]"Characterization of an oligomerization domain and RNA-binding properties on rotavirus nonstructural protein NS34."
Mattion N.M., Cohen J., Aponte C., Estes M.K.
Virology 190:68-83(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M87502 Genomic RNA. Translation: AAA47295.1.

3D structure databases

ProteinModelPortalQ00721.
SMRQ00721. Positions 7-163, 206-315.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ00721. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMSSF69903. SSF69903. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP3_ROTS4
AccessionPrimary (citable) accession number: Q00721
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 16, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families