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Q00721

- NSP3_ROTS4

UniProt

Q00721 - NSP3_ROTS4

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Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain SA11-4F G3-P6[1]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Simian Agent 11 (strain 4F))
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of translation Source: UniProtKB-KW
  2. suppression by virus of host gene expression Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (strain SA11-4F G3-P6[1]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Simian Agent 11 (strain 4F))
Taxonomic identifieri36436 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Non-structural protein 3PRO_0000149542Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ZC3H7BQ9UGR26EBI-1263962,EBI-948845From a different organism.

Protein-protein interaction databases

IntActiQ00721. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ00721.
SMRiQ00721. Positions 7-163, 206-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 149146RNA-bindingBy similarityAdd
BLAST
Regioni150 – 20657DimerizationBy similarityAdd
BLAST
Regioni170 – 23465Interaction with ZC3H7BBy similarityAdd
BLAST
Regioni208 – 315108Interaction with EIF4G1By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili166 – 23772Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.Curated

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

Q00721-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKMESTQQM AVSIINSSFE AAVVAATSAL ENMGIEYDYQ DIYSRVKNKF
60 70 80 90 100
DFVMDDSGVK NNLIGKAITI DQALNNKFGS AIRNRNWLAD TSRPAKLDED
110 120 130 140 150
VNKLRMMLSS KGIDQKMRVL NACFSVKRIP GKSSSIIKCT KLMRDKLERG
160 170 180 190 200
EVEVDDSFVD EKMEVDTIDW KSRYEQLEQR FESLKSRVNE KYNNWVLKAR
210 220 230 240 250
KMNENMHSLQ NVISQQQAHI AELQVYNNKL ERDLQNKIGS LTSSIEWYLR
260 270 280 290 300
SMELDPEIKA DIEQQINSID AINPLHAFDD LESVIRNLIS DYDKLFLMFK
310
GLIQRCNYQY SFGCE
Length:315
Mass (Da):36,451
Last modified:June 1, 1994 - v1
Checksum:i9E5D39FDE6FCE73B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M87502 Genomic RNA. Translation: AAA47295.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M87502 Genomic RNA. Translation: AAA47295.1 .

3D structure databases

ProteinModelPortali Q00721.
SMRi Q00721. Positions 7-163, 206-315.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q00721. 2 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002873. Rotavirus_NSP3.
[Graphical view ]
Pfami PF01665. Rota_NSP3. 1 hit.
[Graphical view ]
SUPFAMi SSF69903. SSF69903. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of an oligomerization domain and RNA-binding properties on rotavirus nonstructural protein NS34."
    Mattion N.M., Cohen J., Aponte C., Estes M.K.
    Virology 190:68-83(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP3_ROTS4
AccessioniPrimary (citable) accession number: Q00721
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3