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Q00715

- H2B1_RAT

UniProt

Q00715 - H2B1_RAT

Protein

Histone H2B type 1

Gene
N/A
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid By similarity.By similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to bacterium Source: UniProtKB-KW
    2. nucleosome assembly Source: InterPro

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone H2B type 1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. nucleosome Source: UniProtKB-KW
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chromosome, Nucleosome core, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 125124Histone H2B type 1PRO_0000071841Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylprolineBy similarity
    Modified residuei6 – 61N6-acetyllysine; alternate1 Publication
    Modified residuei6 – 61N6-crotonyllysine; alternateBy similarity
    Modified residuei12 – 121N6-acetyllysine; alternateBy similarity
    Modified residuei12 – 121N6-crotonyllysine; alternateBy similarity
    Modified residuei13 – 131N6-acetyllysine; alternate1 Publication
    Modified residuei13 – 131N6-crotonyllysine; alternateBy similarity
    Modified residuei15 – 151Phosphoserine; by STK4/MST1By similarity
    Modified residuei16 – 161N6-acetyllysine; alternate1 Publication
    Modified residuei16 – 161N6-crotonyllysine; alternateBy similarity
    Modified residuei17 – 171N6-acetyllysine; alternateBy similarity
    Modified residuei17 – 171N6-crotonyllysine; alternateBy similarity
    Modified residuei21 – 211N6-acetyllysine; alternate1 Publication
    Modified residuei21 – 211N6-crotonyllysine; alternateBy similarity
    Modified residuei24 – 241N6-acetyllysine; alternateBy similarity
    Modified residuei24 – 241N6-crotonyllysine; alternateBy similarity
    Modified residuei35 – 351N6-crotonyllysine; alternateBy similarity
    Cross-linki35 – 35Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
    Modified residuei37 – 371Phosphoserine; by AMPKBy similarity
    Modified residuei47 – 471N6-methyllysineBy similarity
    Modified residuei58 – 581N6,N6-dimethyllysineBy similarity
    Modified residuei79 – 791Dimethylated arginineBy similarity
    Modified residuei85 – 851N6,N6,N6-trimethyllysine; alternateBy similarity
    Modified residuei85 – 851N6-acetyllysine; alternateBy similarity
    Modified residuei86 – 861Omega-N-methylarginineBy similarity
    Modified residuei92 – 921Omega-N-methylarginineBy similarity
    Modified residuei108 – 1081N6-methyllysineBy similarity
    Modified residuei115 – 1151PhosphothreonineBy similarity
    Modified residuei116 – 1161N6-methylated lysineBy similarity
    Cross-linki120 – 120Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Post-translational modificationi

    Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-120 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons By similarity.By similarity
    Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription By similarity.By similarity
    Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes By similarity.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ00715.
    PRIDEiQ00715.

    PTM databases

    PhosphoSiteiQ00715.

    Expressioni

    Gene expression databases

    GenevestigatoriQ00715.

    Interactioni

    Subunit structurei

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

    Protein-protein interaction databases

    BioGridi249180. 2 interactions.
    IntActiQ00715. 1 interaction.
    STRINGi10116.ENSRNOP00000028779.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00715.
    SMRiQ00715. Positions 5-125.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the histone H2B family.Curated

    Phylogenomic databases

    eggNOGiNOG289161.
    HOGENOMiHOG000231213.
    HOVERGENiHBG007774.
    InParanoidiQ00715.
    KOiK11252.
    PhylomeDBiQ00715.

    Family and domain databases

    Gene3Di1.10.20.10. 1 hit.
    InterProiIPR009072. Histone-fold.
    IPR007125. Histone_core_D.
    IPR000558. Histone_H2B.
    [Graphical view]
    PANTHERiPTHR23428. PTHR23428. 1 hit.
    PfamiPF00125. Histone. 1 hit.
    [Graphical view]
    PRINTSiPR00621. HISTONEH2B.
    SMARTiSM00427. H2B. 1 hit.
    [Graphical view]
    SUPFAMiSSF47113. SSF47113. 1 hit.
    PROSITEiPS00357. HISTONE_H2B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00715-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPEPAKSRPA PKKGSKKAVT KAQKKDGKER KRSRKESYSV YVYKVLKQVH    50
    PDTGISSKAM GIMNSFVNDI FERIAGERRL AHYNKRSTIT SREIQTAVRL 100
    LLPGELAKHA VSEGTKAVTK YTSSK 125
    Length:125
    Mass (Da):13,990
    Last modified:January 23, 2007 - v2
    Checksum:i43BF1F86A6DA221A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59961 Genomic DNA. Translation: CAA42585.1.
    PIRiB45945.
    S26185.
    RefSeqiNP_072173.1. NM_022647.1.
    UniGeneiRn.112590.

    Genome annotation databases

    GeneIDi64647.
    KEGGirno:64647.
    UCSCiRGD:621439. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59961 Genomic DNA. Translation: CAA42585.1 .
    PIRi B45945.
    S26185.
    RefSeqi NP_072173.1. NM_022647.1.
    UniGenei Rn.112590.

    3D structure databases

    ProteinModelPortali Q00715.
    SMRi Q00715. Positions 5-125.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249180. 2 interactions.
    IntActi Q00715. 1 interaction.
    STRINGi 10116.ENSRNOP00000028779.

    PTM databases

    PhosphoSitei Q00715.

    Proteomic databases

    PaxDbi Q00715.
    PRIDEi Q00715.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 64647.
    KEGGi rno:64647.
    UCSCi RGD:621439. rat.

    Organism-specific databases

    CTDi 8340.

    Phylogenomic databases

    eggNOGi NOG289161.
    HOGENOMi HOG000231213.
    HOVERGENi HBG007774.
    InParanoidi Q00715.
    KOi K11252.
    PhylomeDBi Q00715.

    Miscellaneous databases

    NextBioi 613637.

    Gene expression databases

    Genevestigatori Q00715.

    Family and domain databases

    Gene3Di 1.10.20.10. 1 hit.
    InterProi IPR009072. Histone-fold.
    IPR007125. Histone_core_D.
    IPR000558. Histone_H2B.
    [Graphical view ]
    PANTHERi PTHR23428. PTHR23428. 1 hit.
    Pfami PF00125. Histone. 1 hit.
    [Graphical view ]
    PRINTSi PR00621. HISTONEH2B.
    SMARTi SM00427. H2B. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47113. SSF47113. 1 hit.
    PROSITEi PS00357. HISTONE_H2B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Presence of a bi-directional S phase-specific transcription regulatory element in the promoter shared by testis-specific TH2A and TH2B histone genes."
      Huh N.E., Hwang I., Lim K., You K.H., Chae C.-B.
      Nucleic Acids Res. 19:93-98(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
    2. "Molecular cloning and differential expression of somatic and testis-specific H2B histone genes during rat spermatogenesis."
      Kim Y.-J., Hwang I., Tres L.L., Kierszenbaum A.L., Chae C.-B.
      Dev. Biol. 124:23-34(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Lubec G., Kang S.U., Lubec S.
      Submitted (SEP-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 36-44; 48-73 AND 100-108, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    4. "Inhibition of core histones acetylation by carcinogenic nickel(II)."
      Golebiowski F., Kasprzak K.S.
      Mol. Cell. Biochem. 279:133-139(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION AT LYS-6; LYS-13; LYS-16 AND LYS-21.

    Entry informationi

    Entry nameiH2B1_RAT
    AccessioniPrimary (citable) accession number: Q00715
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3