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Q00711

- SDHA_YEAST

UniProt

Q00711 - SDHA_YEAST

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Protein
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
Gene
SDH1, SDHA, YKL148C, YKL602
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH1 and SDH2 form the catalytic dimer. Electrons flow from succinate to the FAD bound to SDH1, and sequentially through the iron-sulfur clusters bound to SDH2 and enter the membrane dimer formed by SDH3 and SDH4.

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

FAD.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei266 – 2661FAD By similarity
Binding sitei287 – 2871Substrate By similarity
Binding sitei299 – 2991Substrate By similarity
Active sitei331 – 3311Proton acceptor By similarity
Binding sitei398 – 3981Substrate By similarity
Binding sitei433 – 4331FAD By similarity
Binding sitei444 – 4441Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi59 – 646FAD By similarity
Nucleotide bindingi82 – 9716FAD By similarity
Add
BLAST
Nucleotide bindingi449 – 4502FAD By similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: SGD
  2. protein binding Source: UniProtKB
  3. succinate dehydrogenase (ubiquinone) activity Source: SGD

GO - Biological processi

  1. cellular respiration Source: SGD
  2. mitochondrial electron transport, succinate to ubiquinone Source: SGD
  3. tricarboxylic acid cycle Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:YKL148C-MONOMER.
YEAST:YKL148C-MONOMER.
UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Flavoprotein subunit of complex II
Short name:
FP
Gene namesi
Name:SDH1
Synonyms:SDHA
Ordered Locus Names:YKL148C
ORF Names:YKL602
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL148c.
SGDiS000001631. SDH1.

Subcellular locationi

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side 1 Publication

GO - Cellular componenti

  1. mitochondrial membrane Source: SGD
  2. mitochondrial respiratory chain complex II Source: SGD
  3. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi90 – 901H → S: Abolishes covalent attachment of FAD. No effect on complex assembly. Abolishes succinate-dehydrogenase activity but no effect on fumarate reductase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2828Mitochondrion2 Publications
Add
BLAST
Chaini29 – 640612Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
PRO_0000010342Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei90 – 901Tele-8alpha-FAD histidine By similarity

Proteomic databases

MaxQBiQ00711.
PaxDbiQ00711.
PeptideAtlasiQ00711.
PRIDEiQ00711.

Expressioni

Gene expression databases

GenevestigatoriQ00711.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit. Interacts with EMI5/SDH5; interaction is required for FAD attachment.1 Publication

Protein-protein interaction databases

BioGridi33989. 57 interactions.
DIPiDIP-5853N.
IntActiQ00711. 5 interactions.
MINTiMINT-4817456.
STRINGi4932.YKL148C.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ORZmodel-A29-640[»]
1PB4model-A29-640[»]
ProteinModelPortaliQ00711.
SMRiQ00711. Positions 45-640.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1053.
GeneTreeiENSGT00750000117848.
HOGENOMiHOG000160475.
KOiK00234.
OMAiALRHDTH.
OrthoDBiEOG7V76G3.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00711-1 [UniParc]FASTAAdd to Basket

« Hide

MLSLKKSALS KLTLLRNTRT FTSSALVRQT QGSVNGSASR SADGKYHIID    50
HEYDCVVIGA GGAGLRAAFG LAEAGYKTAC ISKLFPTRSH TVAAQGGINA 100
ALGNMHKDNW KWHMYDTVKG SDWLGDQDSI HYMTREAPKS IIELEHYGVP 150
FSRTENGKIY QRAFGGQTKE YGKGAQAYRT CAVADRTGHA LLHTLYGQAL 200
RHDTHFFIEY FALDLLTHNG EVVGVIAYNQ EDGTIHRFRA HKTIIATGGY 250
GRAYFSCTSA HTCTGDGNAM VSRAGFPLQD LEFVQFHPSG IYGSGCLITE 300
GARGEGGFLV NSEGERFMER YAPTAKDLAC RDVVSRAITM EIREGRGVGK 350
KKDHMYLQLS HLPPEVLKER LPGISETAAI FAGVDVTKEP IPIIPTVHYN 400
MGGIPTKWNG EALTIDEETG EDKVIPGLMA CGEAACVSVH GANRLGANSL 450
LDLVVFGRAV AHTVADTLQP GLPHKPLPSD LGKESIANLD KLRNANGSRS 500
TAEIRMNMKQ TMQKDVSVFR TQSSLDEGVR NITAVEKTFD DVKTTDRSMI 550
WNSDLVETLE LQNLLTCASQ TAVSAANRKE SRGAHAREDY PNRDDEHWMK 600
HTLSWQKDVA APVTLKYRRV IDHTLDEKEC PSVPPTVRAY 640
Length:640
Mass (Da):70,229
Last modified:April 1, 1993 - v1
Checksum:iA657E440B8ED58E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M94874 Genomic DNA. Translation: AAA35024.1.
M86909 Genomic DNA. Translation: AAA35022.1.
M86746 Genomic DNA. Translation: AAA35026.1.
Z26877 Genomic DNA. Translation: CAA81506.1.
Z28148 Genomic DNA. Translation: CAA81989.1.
BK006944 Genomic DNA. Translation: DAA09015.1.
PIRiS34793.
RefSeqiNP_012774.1. NM_001179714.1.

Genome annotation databases

EnsemblFungiiYKL148C; YKL148C; YKL148C.
GeneIDi853709.
KEGGisce:YKL148C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M94874 Genomic DNA. Translation: AAA35024.1 .
M86909 Genomic DNA. Translation: AAA35022.1 .
M86746 Genomic DNA. Translation: AAA35026.1 .
Z26877 Genomic DNA. Translation: CAA81506.1 .
Z28148 Genomic DNA. Translation: CAA81989.1 .
BK006944 Genomic DNA. Translation: DAA09015.1 .
PIRi S34793.
RefSeqi NP_012774.1. NM_001179714.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ORZ model - A 29-640 [» ]
1PB4 model - A 29-640 [» ]
ProteinModelPortali Q00711.
SMRi Q00711. Positions 45-640.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33989. 57 interactions.
DIPi DIP-5853N.
IntActi Q00711. 5 interactions.
MINTi MINT-4817456.
STRINGi 4932.YKL148C.

Proteomic databases

MaxQBi Q00711.
PaxDbi Q00711.
PeptideAtlasi Q00711.
PRIDEi Q00711.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL148C ; YKL148C ; YKL148C .
GeneIDi 853709.
KEGGi sce:YKL148C.

Organism-specific databases

CYGDi YKL148c.
SGDi S000001631. SDH1.

Phylogenomic databases

eggNOGi COG1053.
GeneTreei ENSGT00750000117848.
HOGENOMi HOG000160475.
KOi K00234.
OMAi ALRHDTH.
OrthoDBi EOG7V76G3.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01006 .
BioCyci MetaCyc:YKL148C-MONOMER.
YEAST:YKL148C-MONOMER.

Miscellaneous databases

NextBioi 974713.

Gene expression databases

Genevestigatori Q00711.

Family and domain databases

Gene3Di 1.20.58.100. 1 hit.
3.90.700.10. 1 hit.
InterProi IPR003953. FAD_bind_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view ]
Pfami PF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view ]
SUPFAMi SSF46977. SSF46977. 1 hit.
SSF56425. SSF56425. 1 hit.
TIGRFAMsi TIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEi PS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure, import, and assembly of the yeast homolog of succinate dehydrogenase flavoprotein."
    Schuelke N., Blobel G., Pain D.
    Proc. Natl. Acad. Sci. U.S.A. 89:8011-8015(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-53; 114-136 AND 429-448.
  2. "SDH1, the gene encoding the succinate dehydrogenase flavoprotein subunit from Saccharomyces cerevisiae."
    Chapman K.B., Solomon S.D., Boeke J.D.
    Gene 118:131-136(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Isolation and nucleotide sequence of the Saccharomyces cerevisiae gene for the succinate dehydrogenase flavoprotein subunit."
    Robinson K.M., Lemire B.D.
    J. Biol. Chem. 267:10101-10107(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "Isolation and characterization of the Saccharomyces cerevisiae SDH4 gene encoding a membrane anchor subunit of succinate dehydrogenase."
    Bullis B.L., Lemire B.D.
    J. Biol. Chem. 269:6543-6549(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-38.
    Strain: S288c / GRF88.
  8. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 607-615, SUBCELLULAR LOCATION.
    Strain: ATCC 201238 / W303-1B.
  9. "The covalent attachment of FAD to the flavoprotein of Saccharomyces cerevisiae succinate dehydrogenase is not necessary for import and assembly into mitochondria."
    Robinson K.M., Rothery R.A., Weiner J.H., Lemire B.D.
    Eur. J. Biochem. 222:983-990(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF HIS-90.
  10. "The Saccharomyces cerevisiae mitochondrial succinate:ubiquinone oxidoreductase."
    Lemire B.D., Oyedotun K.S.
    Biochim. Biophys. Acta 1553:102-116(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON SUCCINATE DEHYDROGENASE.
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. Cited for: INTERACTION WITH EMI5.
  13. "The quaternary structure of the Saccharomyces cerevisiae succinate dehydrogenase. Homology modeling, cofactor docking, and molecular dynamics simulation studies."
    Oyedotun K.S., Lemire B.D.
    J. Biol. Chem. 279:9424-9431(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 29-640.

Entry informationi

Entry nameiSDHA_YEAST
AccessioniPrimary (citable) accession number: Q00711
Secondary accession number(s): D6VX49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10400 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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