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Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene

SDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH1 and SDH2 form the catalytic dimer. Electrons flow from succinate to the FAD bound to SDH1, and sequentially through the iron-sulfur clusters bound to SDH2 and enter the membrane dimer formed by SDH3 and SDH4.

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.By similarity

Cofactori

FADBy similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (eukaryal route).By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial (YJL045W), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDH2), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDH1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (eukaryal route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei266FADBy similarity1
Binding sitei287SubstrateBy similarity1
Binding sitei299SubstrateBy similarity1
Active sitei331Proton acceptorBy similarity1
Binding sitei398SubstrateBy similarity1
Binding sitei433FADBy similarity1
Binding sitei444SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi59 – 64FADBy similarity6
Nucleotide bindingi82 – 97FADBy similarityAdd BLAST16
Nucleotide bindingi449 – 450FADBy similarity2

GO - Molecular functioni

  • electron carrier activity Source: GO_Central
  • flavin adenine dinucleotide binding Source: SGD
  • succinate dehydrogenase (ubiquinone) activity Source: SGD

GO - Biological processi

  • anaerobic respiration Source: GO_Central
  • cellular respiration Source: SGD
  • mitochondrial electron transport, succinate to ubiquinone Source: SGD
  • tricarboxylic acid cycle Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:YKL148C-MONOMER.
YEAST:YKL148C-MONOMER.
UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (EC:1.3.5.1By similarity)
Alternative name(s):
Flavoprotein subunit of complex II
Short name:
FP
Gene namesi
Name:SDH1
Synonyms:SDHA
Ordered Locus Names:YKL148C
ORF Names:YKL602
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL148C.
SGDiS000001631. SDH1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial membrane Source: SGD
  • mitochondrial respiratory chain complex II, succinate dehydrogenase complex (ubiquinone) Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi90H → S: Abolishes covalent attachment of FAD. No effect on complex assembly. Abolishes succinate-dehydrogenase activity but no effect on fumarate reductase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28Mitochondrion2 PublicationsAdd BLAST28
ChainiPRO_000001034229 – 640Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialAdd BLAST612

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei90Tele-8alpha-FAD histidineBy similarity1

Proteomic databases

MaxQBiQ00711.
PRIDEiQ00711.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit. Interacts with EMI5/SDH5; interaction is required for FAD attachment.1 Publication

Protein-protein interaction databases

BioGridi33989. 58 interactors.
DIPiDIP-5853N.
IntActiQ00711. 6 interactors.
MINTiMINT-4817456.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ORZmodel-A29-640[»]
1PB4model-A29-640[»]
ProteinModelPortaliQ00711.
SMRiQ00711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00860000134556.
HOGENOMiHOG000160475.
InParanoidiQ00711.
KOiK00234.
OMAiLFKYTAG.
OrthoDBiEOG092C17LB.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR030664. SdhA/FrdA/AprA.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000171. SDHA_APRA_LASPO. 1 hit.
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLKKSALS KLTLLRNTRT FTSSALVRQT QGSVNGSASR SADGKYHIID
60 70 80 90 100
HEYDCVVIGA GGAGLRAAFG LAEAGYKTAC ISKLFPTRSH TVAAQGGINA
110 120 130 140 150
ALGNMHKDNW KWHMYDTVKG SDWLGDQDSI HYMTREAPKS IIELEHYGVP
160 170 180 190 200
FSRTENGKIY QRAFGGQTKE YGKGAQAYRT CAVADRTGHA LLHTLYGQAL
210 220 230 240 250
RHDTHFFIEY FALDLLTHNG EVVGVIAYNQ EDGTIHRFRA HKTIIATGGY
260 270 280 290 300
GRAYFSCTSA HTCTGDGNAM VSRAGFPLQD LEFVQFHPSG IYGSGCLITE
310 320 330 340 350
GARGEGGFLV NSEGERFMER YAPTAKDLAC RDVVSRAITM EIREGRGVGK
360 370 380 390 400
KKDHMYLQLS HLPPEVLKER LPGISETAAI FAGVDVTKEP IPIIPTVHYN
410 420 430 440 450
MGGIPTKWNG EALTIDEETG EDKVIPGLMA CGEAACVSVH GANRLGANSL
460 470 480 490 500
LDLVVFGRAV AHTVADTLQP GLPHKPLPSD LGKESIANLD KLRNANGSRS
510 520 530 540 550
TAEIRMNMKQ TMQKDVSVFR TQSSLDEGVR NITAVEKTFD DVKTTDRSMI
560 570 580 590 600
WNSDLVETLE LQNLLTCASQ TAVSAANRKE SRGAHAREDY PNRDDEHWMK
610 620 630 640
HTLSWQKDVA APVTLKYRRV IDHTLDEKEC PSVPPTVRAY
Length:640
Mass (Da):70,229
Last modified:April 1, 1993 - v1
Checksum:iA657E440B8ED58E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94874 Genomic DNA. Translation: AAA35024.1.
M86909 Genomic DNA. Translation: AAA35022.1.
M86746 Genomic DNA. Translation: AAA35026.1.
Z26877 Genomic DNA. Translation: CAA81506.1.
Z28148 Genomic DNA. Translation: CAA81989.1.
BK006944 Genomic DNA. Translation: DAA09015.1.
PIRiS34793.
RefSeqiNP_012774.1. NM_001179714.1.

Genome annotation databases

EnsemblFungiiYKL148C; YKL148C; YKL148C.
GeneIDi853709.
KEGGisce:YKL148C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94874 Genomic DNA. Translation: AAA35024.1.
M86909 Genomic DNA. Translation: AAA35022.1.
M86746 Genomic DNA. Translation: AAA35026.1.
Z26877 Genomic DNA. Translation: CAA81506.1.
Z28148 Genomic DNA. Translation: CAA81989.1.
BK006944 Genomic DNA. Translation: DAA09015.1.
PIRiS34793.
RefSeqiNP_012774.1. NM_001179714.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ORZmodel-A29-640[»]
1PB4model-A29-640[»]
ProteinModelPortaliQ00711.
SMRiQ00711.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33989. 58 interactors.
DIPiDIP-5853N.
IntActiQ00711. 6 interactors.
MINTiMINT-4817456.

Proteomic databases

MaxQBiQ00711.
PRIDEiQ00711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL148C; YKL148C; YKL148C.
GeneIDi853709.
KEGGisce:YKL148C.

Organism-specific databases

EuPathDBiFungiDB:YKL148C.
SGDiS000001631. SDH1.

Phylogenomic databases

GeneTreeiENSGT00860000134556.
HOGENOMiHOG000160475.
InParanoidiQ00711.
KOiK00234.
OMAiLFKYTAG.
OrthoDBiEOG092C17LB.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.
BioCyciMetaCyc:YKL148C-MONOMER.
YEAST:YKL148C-MONOMER.

Miscellaneous databases

PROiQ00711.

Family and domain databases

Gene3Di1.20.58.100. 1 hit.
3.50.50.60. 2 hits.
3.90.700.10. 1 hit.
InterProiIPR003953. FAD-binding_2.
IPR023753. FAD/NAD-binding_dom.
IPR003952. FRD_SDH_FAD_BS.
IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
IPR030664. SdhA/FrdA/AprA.
IPR027477. Succ_DH/fumarate_Rdtase_cat.
IPR011281. Succ_DH_flav_su_fwd.
IPR014006. Succ_Dhase_FrdA_Gneg.
[Graphical view]
PfamiPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000171. SDHA_APRA_LASPO. 1 hit.
SUPFAMiSSF46977. SSF46977. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF56425. SSF56425. 1 hit.
TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSDHA_YEAST
AccessioniPrimary (citable) accession number: Q00711
Secondary accession number(s): D6VX49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 30, 2016
This is version 174 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.