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Q00711

- SDHA_YEAST

UniProt

Q00711 - SDHA_YEAST

Protein

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gene

SDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 152 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Catalytic subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH1 and SDH2 form the catalytic dimer. Electrons flow from succinate to the FAD bound to SDH1, and sequentially through the iron-sulfur clusters bound to SDH2 and enter the membrane dimer formed by SDH3 and SDH4.

    Catalytic activityi

    Succinate + a quinone = fumarate + a quinol.

    Cofactori

    FAD.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei266 – 2661FADBy similarity
    Binding sitei287 – 2871SubstrateBy similarity
    Binding sitei299 – 2991SubstrateBy similarity
    Active sitei331 – 3311Proton acceptorBy similarity
    Binding sitei398 – 3981SubstrateBy similarity
    Binding sitei433 – 4331FADBy similarity
    Binding sitei444 – 4441SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi59 – 646FADBy similarity
    Nucleotide bindingi82 – 9716FADBy similarityAdd
    BLAST
    Nucleotide bindingi449 – 4502FADBy similarity

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: SGD
    2. protein binding Source: UniProtKB
    3. succinate dehydrogenase (ubiquinone) activity Source: SGD

    GO - Biological processi

    1. cellular respiration Source: SGD
    2. mitochondrial electron transport, succinate to ubiquinone Source: SGD
    3. tricarboxylic acid cycle Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport, Tricarboxylic acid cycle

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciMetaCyc:YKL148C-MONOMER.
    YEAST:YKL148C-MONOMER.
    UniPathwayiUPA00223; UER01006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (EC:1.3.5.1)
    Alternative name(s):
    Flavoprotein subunit of complex II
    Short name:
    FP
    Gene namesi
    Name:SDH1
    Synonyms:SDHA
    Ordered Locus Names:YKL148C
    ORF Names:YKL602
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL148c.
    SGDiS000001631. SDH1.

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Matrix side 1 Publication

    GO - Cellular componenti

    1. mitochondrial membrane Source: SGD
    2. mitochondrial respiratory chain complex II Source: SGD
    3. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi90 – 901H → S: Abolishes covalent attachment of FAD. No effect on complex assembly. Abolishes succinate-dehydrogenase activity but no effect on fumarate reductase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2828Mitochondrion2 PublicationsAdd
    BLAST
    Chaini29 – 640612Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrialPRO_0000010342Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei90 – 901Tele-8alpha-FAD histidineBy similarity

    Proteomic databases

    MaxQBiQ00711.
    PaxDbiQ00711.
    PeptideAtlasiQ00711.
    PRIDEiQ00711.

    Expressioni

    Gene expression databases

    GenevestigatoriQ00711.

    Interactioni

    Subunit structurei

    Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit. Interacts with EMI5/SDH5; interaction is required for FAD attachment.1 Publication

    Protein-protein interaction databases

    BioGridi33989. 57 interactions.
    DIPiDIP-5853N.
    IntActiQ00711. 5 interactions.
    MINTiMINT-4817456.
    STRINGi4932.YKL148C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ORZmodel-A29-640[»]
    1PB4model-A29-640[»]
    ProteinModelPortaliQ00711.
    SMRiQ00711. Positions 45-640.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1053.
    GeneTreeiENSGT00750000117848.
    HOGENOMiHOG000160475.
    KOiK00234.
    OMAiALRHDTH.
    OrthoDBiEOG7V76G3.

    Family and domain databases

    Gene3Di1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProiIPR003953. FAD_bind_dom.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    IPR011281. Succ_DH_flav_su_fwd.
    IPR014006. Succ_Dhase_FrdA_Gneg.
    [Graphical view]
    PfamiPF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsiTIGR01816. sdhA_forward. 1 hit.
    TIGR01812. sdhA_frdA_Gneg. 1 hit.
    PROSITEiPS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00711-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSLKKSALS KLTLLRNTRT FTSSALVRQT QGSVNGSASR SADGKYHIID    50
    HEYDCVVIGA GGAGLRAAFG LAEAGYKTAC ISKLFPTRSH TVAAQGGINA 100
    ALGNMHKDNW KWHMYDTVKG SDWLGDQDSI HYMTREAPKS IIELEHYGVP 150
    FSRTENGKIY QRAFGGQTKE YGKGAQAYRT CAVADRTGHA LLHTLYGQAL 200
    RHDTHFFIEY FALDLLTHNG EVVGVIAYNQ EDGTIHRFRA HKTIIATGGY 250
    GRAYFSCTSA HTCTGDGNAM VSRAGFPLQD LEFVQFHPSG IYGSGCLITE 300
    GARGEGGFLV NSEGERFMER YAPTAKDLAC RDVVSRAITM EIREGRGVGK 350
    KKDHMYLQLS HLPPEVLKER LPGISETAAI FAGVDVTKEP IPIIPTVHYN 400
    MGGIPTKWNG EALTIDEETG EDKVIPGLMA CGEAACVSVH GANRLGANSL 450
    LDLVVFGRAV AHTVADTLQP GLPHKPLPSD LGKESIANLD KLRNANGSRS 500
    TAEIRMNMKQ TMQKDVSVFR TQSSLDEGVR NITAVEKTFD DVKTTDRSMI 550
    WNSDLVETLE LQNLLTCASQ TAVSAANRKE SRGAHAREDY PNRDDEHWMK 600
    HTLSWQKDVA APVTLKYRRV IDHTLDEKEC PSVPPTVRAY 640
    Length:640
    Mass (Da):70,229
    Last modified:April 1, 1993 - v1
    Checksum:iA657E440B8ED58E2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94874 Genomic DNA. Translation: AAA35024.1.
    M86909 Genomic DNA. Translation: AAA35022.1.
    M86746 Genomic DNA. Translation: AAA35026.1.
    Z26877 Genomic DNA. Translation: CAA81506.1.
    Z28148 Genomic DNA. Translation: CAA81989.1.
    BK006944 Genomic DNA. Translation: DAA09015.1.
    PIRiS34793.
    RefSeqiNP_012774.1. NM_001179714.1.

    Genome annotation databases

    EnsemblFungiiYKL148C; YKL148C; YKL148C.
    GeneIDi853709.
    KEGGisce:YKL148C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94874 Genomic DNA. Translation: AAA35024.1 .
    M86909 Genomic DNA. Translation: AAA35022.1 .
    M86746 Genomic DNA. Translation: AAA35026.1 .
    Z26877 Genomic DNA. Translation: CAA81506.1 .
    Z28148 Genomic DNA. Translation: CAA81989.1 .
    BK006944 Genomic DNA. Translation: DAA09015.1 .
    PIRi S34793.
    RefSeqi NP_012774.1. NM_001179714.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ORZ model - A 29-640 [» ]
    1PB4 model - A 29-640 [» ]
    ProteinModelPortali Q00711.
    SMRi Q00711. Positions 45-640.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33989. 57 interactions.
    DIPi DIP-5853N.
    IntActi Q00711. 5 interactions.
    MINTi MINT-4817456.
    STRINGi 4932.YKL148C.

    Proteomic databases

    MaxQBi Q00711.
    PaxDbi Q00711.
    PeptideAtlasi Q00711.
    PRIDEi Q00711.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL148C ; YKL148C ; YKL148C .
    GeneIDi 853709.
    KEGGi sce:YKL148C.

    Organism-specific databases

    CYGDi YKL148c.
    SGDi S000001631. SDH1.

    Phylogenomic databases

    eggNOGi COG1053.
    GeneTreei ENSGT00750000117848.
    HOGENOMi HOG000160475.
    KOi K00234.
    OMAi ALRHDTH.
    OrthoDBi EOG7V76G3.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01006 .
    BioCyci MetaCyc:YKL148C-MONOMER.
    YEAST:YKL148C-MONOMER.

    Miscellaneous databases

    NextBioi 974713.

    Gene expression databases

    Genevestigatori Q00711.

    Family and domain databases

    Gene3Di 1.20.58.100. 1 hit.
    3.90.700.10. 1 hit.
    InterProi IPR003953. FAD_bind_dom.
    IPR003952. FRD_SDH_FAD_BS.
    IPR015939. Fum_Rdtase/Succ_DH_flav-like_C.
    IPR027477. Succ_DH/fumarate_Rdtase_cat.
    IPR011281. Succ_DH_flav_su_fwd.
    IPR014006. Succ_Dhase_FrdA_Gneg.
    [Graphical view ]
    Pfami PF00890. FAD_binding_2. 1 hit.
    PF02910. Succ_DH_flav_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46977. SSF46977. 1 hit.
    SSF56425. SSF56425. 1 hit.
    TIGRFAMsi TIGR01816. sdhA_forward. 1 hit.
    TIGR01812. sdhA_frdA_Gneg. 1 hit.
    PROSITEi PS00504. FRD_SDH_FAD_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure, import, and assembly of the yeast homolog of succinate dehydrogenase flavoprotein."
      Schuelke N., Blobel G., Pain D.
      Proc. Natl. Acad. Sci. U.S.A. 89:8011-8015(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-53; 114-136 AND 429-448.
    2. "SDH1, the gene encoding the succinate dehydrogenase flavoprotein subunit from Saccharomyces cerevisiae."
      Chapman K.B., Solomon S.D., Boeke J.D.
      Gene 118:131-136(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Isolation and nucleotide sequence of the Saccharomyces cerevisiae gene for the succinate dehydrogenase flavoprotein subunit."
      Robinson K.M., Lemire B.D.
      J. Biol. Chem. 267:10101-10107(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
      Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
      Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. "Isolation and characterization of the Saccharomyces cerevisiae SDH4 gene encoding a membrane anchor subunit of succinate dehydrogenase."
      Bullis B.L., Lemire B.D.
      J. Biol. Chem. 269:6543-6549(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 29-38.
      Strain: S288c / GRF88.
    8. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
      Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
      Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 607-615, SUBCELLULAR LOCATION.
      Strain: ATCC 201238 / W303-1B.
    9. "The covalent attachment of FAD to the flavoprotein of Saccharomyces cerevisiae succinate dehydrogenase is not necessary for import and assembly into mitochondria."
      Robinson K.M., Rothery R.A., Weiner J.H., Lemire B.D.
      Eur. J. Biochem. 222:983-990(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF HIS-90.
    10. "The Saccharomyces cerevisiae mitochondrial succinate:ubiquinone oxidoreductase."
      Lemire B.D., Oyedotun K.S.
      Biochim. Biophys. Acta 1553:102-116(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON SUCCINATE DEHYDROGENASE.
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    12. Cited for: INTERACTION WITH EMI5.
    13. "The quaternary structure of the Saccharomyces cerevisiae succinate dehydrogenase. Homology modeling, cofactor docking, and molecular dynamics simulation studies."
      Oyedotun K.S., Lemire B.D.
      J. Biol. Chem. 279:9424-9431(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 29-640.

    Entry informationi

    Entry nameiSDHA_YEAST
    AccessioniPrimary (citable) accession number: Q00711
    Secondary accession number(s): D6VX49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 152 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 10400 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3