Reviewed,
UniProtKB/Swiss-Prot Q00711 (DHSA_YEAST)
Last modified
February 9, 2010.
Version 110.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial EC=1.3.5.1 Alternative name(s): Flavoprotein subunit of complex II Short name=FP | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 640 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalytic subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH1 and SDH2 form the catalytic dimer. Electrons flow from succinate to the FAD bound to SDH1, and sequentially through the iron-sulfur clusters bound to SDH2 and enter the membrane dimer formed by SDH3 and SDH4. |
| Catalytic activity | Succinate + ubiquinone = fumarate + ubiquinol. |
| Cofactor | FAD. |
| Pathway | |
| Subunit structure | Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit. Interacts with EMI5/SDH5; interaction is required for FAD attachment. Ref.11 |
| Subcellular location | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.7. |
| Miscellaneous | Present with 10400 molecules/cell in log phase SD medium. Ref.10 |
| Sequence similarities | Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 28 | 28 | Mitochondrion Ref.6 Ref.1 | ||||||
| Chain | 29 – 640 | 612 | Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial | PRO_0000010342 | |||||
Regions | |||||||||
| Nucleotide binding | 82 – 97 | 16 | FAD By similarity | ||||||
Sites | |||||||||
| Active site | 331 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 287 | 1 | Substrate By similarity | ||||||
| Binding site | 299 | 1 | Substrate By similarity | ||||||
| Binding site | 398 | 1 | Substrate By similarity | ||||||
| Binding site | 444 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 90 | 1 | Tele-8alpha-FAD histidine By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 90 | 1 | H → S: Abolishes covalent attachment of FAD. No effect on complex assembly. Abolishes succinate-dehydrogenase activity but no effect on fumarate reductase activity. Ref.8 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Primary structure, import, and assembly of the yeast homolog of succinate dehydrogenase flavoprotein." Schuelke N., Blobel G., Pain D. Proc. Natl. Acad. Sci. U.S.A. 89:8011-8015(1992) [PubMed: 1518827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-53; 114-136 AND 429-448. |
| [2] | "SDH1, the gene encoding the succinate dehydrogenase flavoprotein subunit from Saccharomyces cerevisiae." Chapman K.B., Solomon S.D., Boeke J.D. Gene 118:131-136(1992) [PubMed: 1511876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Isolation and nucleotide sequence of the Saccharomyces cerevisiae gene for the succinate dehydrogenase flavoprotein subunit." Robinson K.M., Lemire B.D. J. Biol. Chem. 267:10101-10107(1992) [PubMed: 1577780] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [5] | "Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W.Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [6] | "Isolation and characterization of the Saccharomyces cerevisiae SDH4 gene encoding a membrane anchor subunit of succinate dehydrogenase." Bullis B.L., Lemire B.D. J. Biol. Chem. 269:6543-6549(1994) [PubMed: 8120006] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-38. Strain: S288c / GRF88. |
| [7] | "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed: 11502169] [Abstract] Cited for: PROTEIN SEQUENCE OF 607-615, SUBCELLULAR LOCATION. Strain: ATCC 201238 / W303-1B. |
| [8] | "The covalent attachment of FAD to the flavoprotein of Saccharomyces cerevisiae succinate dehydrogenase is not necessary for import and assembly into mitochondria." Robinson K.M., Rothery R.A., Weiner J.H., Lemire B.D. Eur. J. Biochem. 222:983-990(1994) [PubMed: 8026509] [Abstract] Cited for: MUTAGENESIS OF HIS-90. |
| [9] | "The Saccharomyces cerevisiae mitochondrial succinate:ubiquinone oxidoreductase." Lemire B.D., Oyedotun K.S. Biochim. Biophys. Acta 1553:102-116(2002) [PubMed: 11803020] [Abstract] Cited for: REVIEW ON SUCCINATE DEHYDROGENASE. |
| [10] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [11] | "SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma." Hao H.-X., Khalimonchuk O., Schraders M., Dephoure N., Bayley J.-P., Kunst H., Devilee P., Cremers C.W.R.J., Schiffman J.D., Bentz B.G., Gygi S.P., Winge D.R., Kremer H., Rutter J. Science 325:1139-1142(2009) [PubMed: 19628817] [Abstract] Cited for: INTERACTION WITH EMI5. |
| [12] | "The quaternary structure of the Saccharomyces cerevisiae succinate dehydrogenase. Homology modeling, cofactor docking, and molecular dynamics simulation studies." Oyedotun K.S., Lemire B.D. J. Biol. Chem. 279:9424-9431(2004) [PubMed: 14672929] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 29-640. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | M94874 Genomic DNA. Translation: AAA35024.1. M86909 Genomic DNA. Translation: AAA35022.1. M86746 Genomic DNA. Translation: AAA35026.1. Z26877 Genomic DNA. Translation: CAA81506.1. Z28148 Genomic DNA. Translation: CAA81989.1. | ||||||||||||||||||
| PIR | S34793. | ||||||||||||||||||
| RefSeq | NP_012774.1. | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| PDBe RCSB PDB PDBj |
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| SMR | Q00711. Positions 45-640. | ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| DIP | DIP-5853N. | ||||||||||||||||||
| IntAct | Q00711. 6 interactions. | ||||||||||||||||||
| STRING | Q00711. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PeptideAtlas | Q00711. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | YKL148C; YKL148C; YKL148C; Saccharomyces cerevisiae. [Genome view] | ||||||||||||||||||
| GeneID | 853709. | ||||||||||||||||||
| KEGG | sce:YKL148C. | ||||||||||||||||||
| NMPDR | fig|4932.3.peg.3753. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| CYGD | YKL148c. | ||||||||||||||||||
| SGD | S000001631. SDH1. | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| eggNOG | fuNOG04691. | ||||||||||||||||||
| HOGENOM | HBG293998. | ||||||||||||||||||
| OMA | CVGVIAY. | ||||||||||||||||||
| OrthoDB | EOG918D4W. | ||||||||||||||||||
| PhylomeDB | Q00711. | ||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||
| BRENDA | 1.3.5.1. 250. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | Q00711. | ||||||||||||||||||
| Genevestigator | Q00711. | ||||||||||||||||||
| GermOnline | YKL148C. Saccharomyces cerevisiae. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR011281. Succ_DH_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view] | ||||||||||||||||||
| Pfam | PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view] | ||||||||||||||||||
| PRINTS | PR00368. FADPNR. | ||||||||||||||||||
| TIGRFAMs | TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit. | ||||||||||||||||||
| PROSITE | PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| NextBio | 974713. | ||||||||||||||||||
Entry information
| Entry name | DHSA_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q00711 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XI Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names |

Clusters with


