Apoptosis regulator Bcl-2 - Gallus gallus (Chicken)

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Q00709 (BCL2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Apoptosis regulator Bcl-2

Gene names

Name:	BCL2
Synonyms:	BCL-2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Subunit structure	Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity By similarity. Also interacts with APAF1 and RAF-1 By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
Tissue specificity	In adult chicken expressed, in thymus, spleen, kidney, heart, ovary and brain, with the highest levels in the thymus. In the embryo, highly levels expressed in all tissues with high levels in the bursa of Fabricius.
Domain	The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1 By similarity.
Sequence similarities	Belongs to the Bcl-2 family.
Sequence caution	The sequence CAA78018.1 differs from that shown. Reason: Frameshift at positions 63 and 82.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane Nucleus
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anti-apoptosis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cellular pH reduction Inferred from sequence or structural similarity. Source: UniProtKB regulation of mitochondrial membrane permeability Inferred from sequence or structural similarity. Source: HGNC regulation of mitochondrial membrane potential Inferred from sequence or structural similarity. Source: HGNC release of cytochrome c from mitochondria Inferred from sequence or structural similarity. Source: HGNC
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from sequence or structural similarity. Source: UniProtKB nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 233

233

Apoptosis regulator Bcl-2

PRO_0000143051

Regions

Transmembrane

208 – 228

Helical; Potential

Motif

10 – 30

BH4

Motif

87 – 101

BH3

Motif

130 – 149

BH1

Motif

181 – 196

BH2

Experimental info

Sequence conflict

121

H → T in CAA78018. Ref.2

Sequence conflict

139

G → V in CAA78018. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q00709 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 5252555ACB6E4C3D
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FASTA

233

25,687

        10         20         30         40         50         60 
MAHPGRRGYD NREIVLKYIH YKLSQRGYDW AAGEDRPPVP PAPAPAAAPA AVAAAGASSH 

        70         80         90        100        110        120 
HRPEPPGSAA ASEVPPAEGL RPAPPGVHLA LRQAGDEFSR RYQRDFAQMS GQLHLTPFTA 

       130        140        150        160        170        180 
HGRFVAVVEE LFRDGVNWGR IVAFFEFGGV MCVESVNREM SPLVDNIATW MTEYLNRHLH 

       190        200        210        220        230 
NWIQDNGGWD AFVELYGNSM RPLFDFSWIS LKTILSLVLV GACITLGAYL GHK

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References

[1]	"Isolation and characterization of the chicken bcl-2 gene: expression in a variety of tissues including lymphoid and neuronal organs in adult and embryo." Eguchi Y., Ewert D.L., Tsujimoto Y. Nucleic Acids Res. 20:4187-4192(1992) [PubMed: 1508712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and DNA sequence analysis of cDNA encoding chicken homologue of the Bcl-2 oncoprotein." Cazals-Hatem D.L., Louie D.C., Tanaka S., Reed J.C. Biochim. Biophys. Acta 1132:109-113(1992) [PubMed: 1511008] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: B-cell lymphoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11382 Genomic DNA. Translation: BAA01978.1. Z11961 mRNA. Translation: CAA78018.1. Frameshift.
IPI	IPI00601515.
PIR	A37332. S24390.
RefSeq	NP_990670.1. NM_205339.1.
UniGene	Gga.43243.
3D structure databases
ProteinModelPortal	Q00709.
SMR	Q00709. Positions 3-201.
ModBase	Search...
Protein-protein interaction databases
STRING	Q00709.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000021014; ENSGALP00000020984; ENSGALG00000012885.
GeneID	396282.
KEGG	gga:396282.
Organism-specific databases
CTD	596.
Phylogenomic databases
eggNOG	veNOG14794.
GeneTree	ENSGT00530000062935.
HOGENOM	HBG717457.
HOVERGEN	HBG004472.
InParanoid	Q00709.
OMA	EWDAGDA.
PhylomeDB	Q00709.
Family and domain databases
InterPro	IPR013278. Apop_reg_Bcl2. IPR002475. Bcl2-like_apoptosis. IPR000712. Bcl2_BH. IPR020717. Bcl2_BH1_motif_CS. IPR020726. Bcl2_BH2_motif_CS. IPR020728. Bcl2_BH3_motif_CS. IPR003093. Bcl2_BH4. IPR020731. Bcl2_BH4_motif_CS. IPR004725. Bcl2_reg. [Graphical view]
KO	K02161.
Pfam	PF00452. Bcl-2. 1 hit. PF02180. BH4. 1 hit. [Graphical view]
PRINTS	PR01863. APOPREGBCL2. PR01862. BCL2FAMILY.
SMART	SM00337. BCL. 1 hit. SM00265. BH4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00865. Bcl-2. 1 hit.
PROSITE	PS50062. BCL2_FAMILY. 1 hit. PS01080. BH1. 1 hit. PS01258. BH2. 1 hit. PS01259. BH3. 1 hit. PS01260. BH4_1. 1 hit. PS50063. BH4_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BCL2_CHICK

Accession

Primary (citable) accession number: Q00709

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 16, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents