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Q00690 (LYAM2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E-selectin
Alternative name(s):
CD62 antigen-like family member E
Endothelial leukocyte adhesion molecule 1
Short name=ELAM-1
Leukocyte-endothelial cell adhesion molecule 2
Short name=LECAM2
CD_antigen=CD62E
Gene names
Name:Sele
Synonyms:Elam-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length612 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.

Subunit structure

Interacts with PSGL1/SELPLG and PODXL2 through the sialyl Lewis X epitope. PSGL1 sulfation appears not to be required for this interaction By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the selectin/LECAM family.

Contains 1 C-type lectin domain.

Contains 1 EGF-like domain.

Contains 6 Sushi (CCP/SCR) domains.

Sequence caution

The sequence AAA37577.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentMembrane
   DomainEGF-like domain
Repeat
Signal
Sushi
Transmembrane
Transmembrane helix
   LigandLectin
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin filament-based process

Inferred from electronic annotation. Source: Ensembl

activation of phospholipase C activity

Inferred from electronic annotation. Source: Ensembl

heterophilic cell-cell adhesion

Inferred from electronic annotation. Source: Ensembl

leukocyte tethering or rolling

Inferred from electronic annotation. Source: Ensembl

positive regulation of leukocyte migration

Inferred from genetic interaction PubMed 17114491. Source: MGI

positive regulation of receptor internalization

Inferred from electronic annotation. Source: Ensembl

response to interleukin-1

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcaveola

Inferred from electronic annotation. Source: Ensembl

coated pit

Inferred from electronic annotation. Source: Ensembl

cortical cytoskeleton

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionoligosaccharide binding

Inferred from electronic annotation. Source: Ensembl

sialic acid binding

Inferred from electronic annotation. Source: Ensembl

transmembrane signaling receptor activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 By similarity
Chain22 – 612591E-selectin
PRO_0000017493

Regions

Topological domain22 – 557536Extracellular Potential
Transmembrane558 – 57922Helical; Potential
Topological domain580 – 61233Cytoplasmic Potential
Domain22 – 139118C-type lectin
Domain140 – 17536EGF-like
Domain178 – 24063Sushi 1
Domain241 – 30262Sushi 2
Domain303 – 36563Sushi 3
Domain366 – 42863Sushi 4
Domain430 – 49162Sushi 5
Domain492 – 55059Sushi 6

Amino acid modifications

Glycosylation251N-linked (GlcNAc...) Potential
Glycosylation1451N-linked (GlcNAc...) Potential
Glycosylation1921N-linked (GlcNAc...) Potential
Glycosylation2031N-linked (GlcNAc...) Potential
Glycosylation2661N-linked (GlcNAc...) Potential
Glycosylation3331N-linked (GlcNAc...) Potential
Glycosylation3911N-linked (GlcNAc...) Potential
Glycosylation5041N-linked (GlcNAc...) Potential
Glycosylation5281N-linked (GlcNAc...) Potential
Disulfide bond40 ↔ 138 By similarity
Disulfide bond111 ↔ 130 By similarity
Disulfide bond143 ↔ 154 By similarity
Disulfide bond148 ↔ 163 By similarity
Disulfide bond165 ↔ 174 By similarity
Disulfide bond180 ↔ 225 By similarity
Disulfide bond210 ↔ 238 By similarity
Disulfide bond243 ↔ 287 By similarity
Disulfide bond273 ↔ 300 By similarity
Disulfide bond305 ↔ 350 By similarity
Disulfide bond336 ↔ 363 By similarity
Disulfide bond368 ↔ 413 By similarity
Disulfide bond399 ↔ 426 By similarity
Disulfide bond431 ↔ 476 By similarity
Disulfide bond462 ↔ 489 By similarity
Disulfide bond494 ↔ 535 By similarity
Disulfide bond521 ↔ 548 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q00690 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 86F05713F0EC2C3D

FASTA61266,750
        10         20         30         40         50         60 
MNASRFLSAL VFVLLAGEST AWYYNASSEL MTYDEASAYC QRDYTHLVAI QNKEEINYLN 

        70         80         90        100        110        120 
SNLKHSPSYY WIGIRKVNNV WIWVGTGKPL TEEAQNWAPG EPNNKQRNED CVEIYIQRTK 

       130        140        150        160        170        180 
DSGMWNDERC NKKKLALCYT ASCTNASCSG HGECIETINS YTCKCHPGFL GPNCEQAVTC 

       190        200        210        220        230        240 
KPQEHPDYGS LNCSHPFGPF SYNSSCSFGC KRGYLPSSME TTVRCTSSGE WSAPAPACHV 

       250        260        270        280        290        300 
VECEALTHPA HGIRKCSSNP GSYPWNTTCT FDCVEGYRRV GAQNLQCTSS GIWDNETPSC 

       310        320        330        340        350        360 
KAVTCDAIPQ PQNGFVSCSH STAGELAFKS SCNFTCEQSF TLQGPAQVEC SAQGQWTPQI 

       370        380        390        400        410        420 
PVCKAVQCEA LSAPQQGNMK CLPSASGPFQ NGSSCEFSCE EGFELKGSRR LQCGPRGEWD 

       430        440        450        460        470        480 
SKKPTCSAVK CDDVPRPQNG VMECAHATTG EFTYKSSCAF QCNEGFSLHG SAQLECTSQG 

       490        500        510        520        530        540 
KWTQEVPSCQ VVQCPSLDVP GKMNMSCSGT AVFGTVCEFT CPDDWTLNGS AVLTCGATGR 

       550        560        570        580        590        600 
WSGMPPTCEA PVSPTRPLVV ALSAAGTSLL TSSSLLYLLM RYFRKKAKKF VPASSCQSLQ 

       610 
SFENYHVPSY NV 

« Hide

References

[1]"Murine endothelial leukocyte-adhesion molecule 1 is a close structural and functional homologue of the human protein."
Becker-Andre M., van Huijsduijnen R.H., Losberger C., Whelan J., Delamarter J.F.
Eur. J. Biochem. 206:401-411(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha."
Weller A., Isenmann S., Vestweber D.
J. Biol. Chem. 267:15176-15183(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M80778 Genomic DNA. Translation: AAA37547.1.
M87862 mRNA. Translation: AAA37577.1. Different initiation.
PIRB42755. S23174.
RefSeqNP_035475.1. NM_011345.2.
XP_006496778.1. XM_006496715.1.
UniGeneMm.5245.

3D structure databases

ProteinModelPortalQ00690.
SMRQ00690. Positions 22-178, 189-561.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59328N.
STRING10090.ENSMUSP00000027874.

Chemistry

BindingDBQ00690.
ChEMBLCHEMBL2545.

PTM databases

PhosphoSiteQ00690.

Proteomic databases

PaxDbQ00690.
PRIDEQ00690.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID20339.
KEGGmmu:20339.

Organism-specific databases

CTD6401.
MGIMGI:98278. Sele.

Phylogenomic databases

eggNOGNOG242963.
HOVERGENHBG052375.
InParanoidQ00690.
KOK06494.
PhylomeDBQ00690.

Gene expression databases

BgeeQ00690.
CleanExMM_SELE.
GenevestigatorQ00690.

Family and domain databases

Gene3D3.10.100.10. 1 hit.
InterProIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR002396. Selectin_superfamily.
IPR000436. Sushi_SCR_CCP.
[Graphical view]
PfamPF00008. EGF. 1 hit.
PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 6 hits.
[Graphical view]
PRINTSPR00343. SELECTIN.
SMARTSM00032. CCP. 6 hits.
SM00034. CLECT. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMSSF56436. SSF56436. 1 hit.
SSF57535. SSF57535. 6 hits.
PROSITEPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50923. SUSHI. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio298157.
PROQ00690.
SOURCESearch...

Entry information

Entry nameLYAM2_MOUSE
AccessionPrimary (citable) accession number: Q00690
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot