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Protein

E-selectin

Gene

Sele

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.

GO - Molecular functioni

  1. oligosaccharide binding Source: MGI
  2. phospholipase binding Source: MGI
  3. sialic acid binding Source: MGI
  4. transmembrane signaling receptor activity Source: MGI

GO - Biological processi

  1. actin filament-based process Source: MGI
  2. activation of phospholipase C activity Source: MGI
  3. heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
  4. leukocyte cell-cell adhesion Source: MGI
  5. leukocyte tethering or rolling Source: MGI
  6. positive regulation of leukocyte migration Source: MGI
  7. positive regulation of receptor internalization Source: MGI
  8. response to interleukin-1 Source: MGI
  9. signal transduction Source: GOC
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiREACT_278886. Cell surface interactions at the vascular wall.

Names & Taxonomyi

Protein namesi
Recommended name:
E-selectin
Alternative name(s):
CD62 antigen-like family member E
Endothelial leukocyte adhesion molecule 1
Short name:
ELAM-1
Leukocyte-endothelial cell adhesion molecule 2
Short name:
LECAM2
CD_antigen: CD62E
Gene namesi
Name:Sele
Synonyms:Elam-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98278. Sele.

Subcellular locationi

  1. Cell membrane By similarity; Single-pass type I membrane protein

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 557536ExtracellularSequence AnalysisAdd
BLAST
Transmembranei558 – 57922HelicalSequence AnalysisAdd
BLAST
Topological domaini580 – 61233CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. caveola Source: MGI
  2. coated pit Source: MGI
  3. cortical cytoskeleton Source: MGI
  4. extracellular space Source: MGI
  5. integral component of membrane Source: UniProtKB-KW
  6. membrane raft Source: MGI
  7. perinuclear region of cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121By similarityAdd
BLAST
Chaini22 – 612591E-selectinPRO_0000017493Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi40 ↔ 138By similarity
Disulfide bondi111 ↔ 130By similarity
Disulfide bondi143 ↔ 154By similarity
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi148 ↔ 163By similarity
Disulfide bondi165 ↔ 174By similarity
Disulfide bondi180 ↔ 225By similarity
Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi210 ↔ 238By similarity
Disulfide bondi243 ↔ 287By similarity
Glycosylationi266 – 2661N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi273 ↔ 300By similarity
Disulfide bondi305 ↔ 350By similarity
Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi336 ↔ 363By similarity
Disulfide bondi368 ↔ 413By similarity
Glycosylationi391 – 3911N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi399 ↔ 426By similarity
Disulfide bondi431 ↔ 476By similarity
Disulfide bondi462 ↔ 489By similarity
Disulfide bondi494 ↔ 535By similarity
Glycosylationi504 – 5041N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi521 ↔ 548By similarity
Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ00690.
PRIDEiQ00690.

PTM databases

PhosphoSiteiQ00690.

Expressioni

Gene expression databases

BgeeiQ00690.
CleanExiMM_SELE.
ExpressionAtlasiQ00690. baseline and differential.
GenevestigatoriQ00690.

Interactioni

Subunit structurei

Interacts with PSGL1/SELPLG and PODXL2 through the sialyl Lewis X epitope. PSGL1 sulfation appears not to be required for this interaction (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-59328N.
STRINGi10090.ENSMUSP00000027874.

Structurei

3D structure databases

ProteinModelPortaliQ00690.
SMRiQ00690. Positions 22-561.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 139118C-type lectinPROSITE-ProRule annotationAdd
BLAST
Domaini140 – 17536EGF-likePROSITE-ProRule annotationAdd
BLAST
Domaini178 – 24063Sushi 1PROSITE-ProRule annotationAdd
BLAST
Domaini241 – 30262Sushi 2PROSITE-ProRule annotationAdd
BLAST
Domaini303 – 36563Sushi 3PROSITE-ProRule annotationAdd
BLAST
Domaini366 – 42863Sushi 4PROSITE-ProRule annotationAdd
BLAST
Domaini430 – 49162Sushi 5PROSITE-ProRule annotationAdd
BLAST
Domaini492 – 55059Sushi 6PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the selectin/LECAM family.Curated
Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 6 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG242963.
HOVERGENiHBG052375.
InParanoidiQ00690.
KOiK06494.
PhylomeDBiQ00690.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR002396. Selectin_superfamily.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 6 hits.
[Graphical view]
PRINTSiPR00343. SELECTIN.
SMARTiSM00032. CCP. 6 hits.
SM00034. CLECT. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
SSF57535. SSF57535. 6 hits.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50923. SUSHI. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00690-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNASRFLSAL VFVLLAGEST AWYYNASSEL MTYDEASAYC QRDYTHLVAI
60 70 80 90 100
QNKEEINYLN SNLKHSPSYY WIGIRKVNNV WIWVGTGKPL TEEAQNWAPG
110 120 130 140 150
EPNNKQRNED CVEIYIQRTK DSGMWNDERC NKKKLALCYT ASCTNASCSG
160 170 180 190 200
HGECIETINS YTCKCHPGFL GPNCEQAVTC KPQEHPDYGS LNCSHPFGPF
210 220 230 240 250
SYNSSCSFGC KRGYLPSSME TTVRCTSSGE WSAPAPACHV VECEALTHPA
260 270 280 290 300
HGIRKCSSNP GSYPWNTTCT FDCVEGYRRV GAQNLQCTSS GIWDNETPSC
310 320 330 340 350
KAVTCDAIPQ PQNGFVSCSH STAGELAFKS SCNFTCEQSF TLQGPAQVEC
360 370 380 390 400
SAQGQWTPQI PVCKAVQCEA LSAPQQGNMK CLPSASGPFQ NGSSCEFSCE
410 420 430 440 450
EGFELKGSRR LQCGPRGEWD SKKPTCSAVK CDDVPRPQNG VMECAHATTG
460 470 480 490 500
EFTYKSSCAF QCNEGFSLHG SAQLECTSQG KWTQEVPSCQ VVQCPSLDVP
510 520 530 540 550
GKMNMSCSGT AVFGTVCEFT CPDDWTLNGS AVLTCGATGR WSGMPPTCEA
560 570 580 590 600
PVSPTRPLVV ALSAAGTSLL TSSSLLYLLM RYFRKKAKKF VPASSCQSLQ
610
SFENYHVPSY NV
Length:612
Mass (Da):66,750
Last modified:April 1, 1993 - v1
Checksum:i86F05713F0EC2C3D
GO

Sequence cautioni

The sequence AAA37577.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80778 Genomic DNA. Translation: AAA37547.1.
M87862 mRNA. Translation: AAA37577.1. Different initiation.
PIRiS23174. B42755.
RefSeqiNP_035475.1. NM_011345.2.
XP_006496778.1. XM_006496715.2.
UniGeneiMm.5245.

Genome annotation databases

GeneIDi20339.
KEGGimmu:20339.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

E-selectin

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80778 Genomic DNA. Translation: AAA37547.1.
M87862 mRNA. Translation: AAA37577.1. Different initiation.
PIRiS23174. B42755.
RefSeqiNP_035475.1. NM_011345.2.
XP_006496778.1. XM_006496715.2.
UniGeneiMm.5245.

3D structure databases

ProteinModelPortaliQ00690.
SMRiQ00690. Positions 22-561.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59328N.
STRINGi10090.ENSMUSP00000027874.

Chemistry

BindingDBiQ00690.
ChEMBLiCHEMBL2545.

PTM databases

PhosphoSiteiQ00690.

Proteomic databases

PaxDbiQ00690.
PRIDEiQ00690.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20339.
KEGGimmu:20339.

Organism-specific databases

CTDi6401.
MGIiMGI:98278. Sele.

Phylogenomic databases

eggNOGiNOG242963.
HOVERGENiHBG052375.
InParanoidiQ00690.
KOiK06494.
PhylomeDBiQ00690.

Enzyme and pathway databases

ReactomeiREACT_278886. Cell surface interactions at the vascular wall.

Miscellaneous databases

NextBioi298157.
PROiQ00690.
SOURCEiSearch...

Gene expression databases

BgeeiQ00690.
CleanExiMM_SELE.
ExpressionAtlasiQ00690. baseline and differential.
GenevestigatoriQ00690.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR002396. Selectin_superfamily.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 6 hits.
[Graphical view]
PRINTSiPR00343. SELECTIN.
SMARTiSM00032. CCP. 6 hits.
SM00034. CLECT. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
SSF57535. SSF57535. 6 hits.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50923. SUSHI. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Murine endothelial leukocyte-adhesion molecule 1 is a close structural and functional homologue of the human protein."
    Becker-Andre M., van Huijsduijnen R.H., Losberger C., Whelan J., Delamarter J.F.
    Eur. J. Biochem. 206:401-411(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha."
    Weller A., Isenmann S., Vestweber D.
    J. Biol. Chem. 267:15176-15183(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiLYAM2_MOUSE
AccessioniPrimary (citable) accession number: Q00690
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 29, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.