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Q00684 (CDC14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein phosphatase CDC14
EC=3.1.3.48
Gene names
Name:CDC14
Synonyms:OAF3
Ordered Locus Names:YFR028C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Required also for mitotic exit, where it is required for the termination of M phase. Activity is inhibited by interaction with NET1 which sequesters it to the nucleolus. Ref.2 Ref.7 Ref.8

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Component of the RENT (regulator of nucleolar silencing and telophase) complex which is composed of at least NET1, CDC14 and SIR2. Ref.7

Subcellular location

Nucleus › nucleolus. Cytoplasm Ref.8.

Miscellaneous

Present with 8550 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.

Sequence caution

The sequence AAA34477.1 differs from that shown. Reason: Frameshift at positions 116 and 190.

The sequence CAA52971.1 differs from that shown. Reason: Frameshift at position 117.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 551551Tyrosine-protein phosphatase CDC14
PRO_0000094875

Sites

Active site2831Phosphocysteine intermediate By similarity

Amino acid modifications

Modified residue2711Phosphothreonine Ref.11
Modified residue4671Phosphoserine Ref.10 Ref.12

Experimental info

Sequence conflict1181A → P in BAA09533. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q00684 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 4EB3985DFA3FD823

FASTA55161,907
        10         20         30         40         50         60 
MRRSVYLDNT IEFLRGRVYL GAYDYTPEDT DELVFFTVED AIFYNSFHLD FGPMNIGHLY 

        70         80         90        100        110        120 
RFAVIFHEIL NDPENANKAV VFYSSASTRQ RANAACMLCC YMILVQAWTP HQVLQPLAQV 

       130        140        150        160        170        180 
DPPFMPFRDA GYSNADFEIT IQDVVYGVWR AKEKGLIDLH SFNLESYEKY EHVEFGDFNV 

       190        200        210        220        230        240 
LTPDFIAFAS PQEDHPKGYL ATKSSHLNQP FKSVLNFFAN NNVQLVVRLN SHLYNKKHFE 

       250        260        270        280        290        300 
DIGIQHLDLI FEDGTCPDLS IVKNFVGAAE TIIKRGGKIA VHCKAGLGRT GCLIGAHLIY 

       310        320        330        340        350        360 
TYGFTANECI GFLRFIRPGM VVGPQQHWLY LHQNDFREWK YTTRISLKPS EAIGGLYPLI 

       370        380        390        400        410        420 
SLEEYRLQKK KLKDDKRVAQ NNIEGELRDL TMTPPSNGHG ALSARNSSQP STANNGSNSF 

       430        440        450        460        470        480 
KSSAVPQTSP GQPRKGQNGS NTIEDINNNR NPTSHANRKV VIESNNSDDE SMQDTNGTSN 

       490        500        510        520        530        540 
HYPKVSRKKN DISSASSSRM EDNEPSATNI NNAADDTILR QLLPKNRRVT SGRRTTSAAG 

       550 
GIRKISGSIK K

« Hide

References

« Hide 'large scale' references
[1]"CDC14 of Saccharomyces cerevisiae. Cloning, sequence analysis, and transcription during the cell cycle."
Wan J., Xu H., Grunstein M.
J. Biol. Chem. 267:11274-11280(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Dominant mutant alleles of yeast protein kinase gene CDC15 suppress the lte1 defect in termination of M phase and genetically interact with CDC14."
Shirayama M., Matsui Y., Toh-e A.
Mol. Gen. Genet. 251:176-185(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[6]Mai B., Lipp M.
Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-421.
[7]"Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit."
Shou W., Sakamoto K.M., Keener J., Morimoto K.W., Traverso E.E., Azzam R., Hoppe G.J., Feldman R.M.R., DeModena J., Moazed D., Charbonneau H., Nomura M., Deshaies R.J.
Mol. Cell 8:45-55(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[8]"The Cdc14 phosphatase and the FEAR network control meiotic spindle disassembly and chromosome segregation."
Marston A.L., Lee B.H., Amon A.
Dev. Cell 4:711-726(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-467, MASS SPECTROMETRY.
Strain: ADR376.
[11]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-271, MASS SPECTROMETRY.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-467, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M61194 Genomic DNA. Translation: AAA34477.1. Frameshift.
D55715 Genomic DNA. Translation: BAA09533.1.
D50617 Genomic DNA. Translation: BAA09267.1.
X75077 Genomic DNA. Translation: CAA52971.1. Frameshift.
BK006940 Genomic DNA. Translation: DAA12468.1.
PIRS56283.
RefSeqNP_116684.3. NM_001179993.3.

3D structure databases

ProteinModelPortalQ00684.
SMRQ00684. Positions 5-336.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5116N.
IntActQ00684. 160 interactions.
MINTMINT-564685.

Proteomic databases

PaxDbQ00684.
PeptideAtlasQ00684.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFR028C; YFR028C; YFR028C.
GeneID850585.
KEGGsce:YFR028C.

Organism-specific databases

CYGDYFR028c.
SGDS000001924. CDC14.

Phylogenomic databases

eggNOGCOG2453.
GeneTreeENSGT00390000010254.
HOGENOMHOG000198341.
KOK06639.
OMAANECIGF.
OrthoDBEOG4MD17S.

Enzyme and pathway databases

BioCycYEAST:G3O-30477-MONOMER.
SABIO-RKQ00684.

Gene expression databases

GenevestigatorQ00684.
GermOnlineYFR028C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR026070. CDC14.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERPTHR23339:SF22. PTHR23339:SF22. 1 hit.
PfamPF00782. DSPc. 1 hit.
[Graphical view]
SMARTSM00195. DSPc. 1 hit.
[Graphical view]
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966423.

Entry information

Entry nameCDC14_YEAST
AccessionPrimary (citable) accession number: Q00684
Secondary accession number(s): D6VTQ8, Q05180, Q05673
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1995
Last modified: May 29, 2013
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents