Q00684 (CDC14_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 130.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Tyrosine-protein phosphatase CDC14 EC=3.1.3.48 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome] | ||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › ![]() |
Protein attributes
| Sequence length | 551 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Required also for mitotic exit, where it is required for the termination of M phase. Activity is inhibited by interaction with NET1 which sequesters it to the nucleolus. Ref.2 Ref.7 Ref.8 |
| Catalytic activity | Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. |
| Subunit structure | Component of the RENT (regulator of nucleolar silencing and telophase) complex which is composed of at least NET1, CDC14 and SIR2. Ref.7 |
| Subcellular location | |
| Miscellaneous | Present with 8550 molecules/cell in log phase SD medium. |
| Sequence similarities | Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily. |
| Sequence caution | The sequence AAA34477.1 differs from that shown. Reason: Frameshift at positions 116 and 190. The sequence CAA52971.1 differs from that shown. Reason: Frameshift at position 117. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ASK1 | P35734 | 2 | EBI-4192,EBI-26682 | |
| BCK1 | Q01389 | 4 | EBI-4192,EBI-3470 | |
| BOI1 | P38041 | 2 | EBI-4192,EBI-3719 | |
| CBK1 | P53894 | 2 | EBI-4192,EBI-4110 | |
| CDC5 | P32562 | 2 | EBI-4192,EBI-4440 | |
| CHK1 | P38147 | 2 | EBI-4192,EBI-4593 | |
| CKA1 | P15790 | 3 | EBI-4192,EBI-9533 | |
| CKA2 | P19454 | 3 | EBI-4192,EBI-9548 | |
| CLB3 | P24870 | 2 | EBI-4192,EBI-4522 | |
| FIN1 | Q03898 | 2 | EBI-4192,EBI-32941 | |
| FMP48 | P53233 | 2 | EBI-4192,EBI-9664 | |
| GIC1 | P38785 | 2 | EBI-4192,EBI-7575 | |
| MCK1 | P21965 | 2 | EBI-4192,EBI-10517 | |
| MET14 | Q02196 | 2 | EBI-4192,EBI-9485 | |
| NET1 | P47035 | 13 | EBI-4192,EBI-25953 | |
| ORC6 | P38826 | 2 | EBI-4192,EBI-12588 | |
| RSP5 | P39940 | 2 | EBI-4192,EBI-16219 | |
| SAK1 | P38990 | 2 | EBI-4192,EBI-12863 | |
| SIC1 | P38634 | 2 | EBI-4192,EBI-17127 | |
| SIR2 | P06700 | 5 | EBI-4192,EBI-17219 | |
| SLI15 | P38283 | 2 | EBI-4192,EBI-20842 | |
| SNF1 | P06782 | 2 | EBI-4192,EBI-17516 | |
| STE7 | P06784 | 2 | EBI-4192,EBI-18389 | |
| SWE1 | P32944 | 3 | EBI-4192,EBI-18607 | |
| TOF2 | Q02208 | 8 | EBI-4192,EBI-27048 | |
| VHS1 | Q03785 | 2 | EBI-4192,EBI-35568 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 551 | 551 | Tyrosine-protein phosphatase CDC14 | PRO_0000094875 | |||||
Sites | |||||||||
| Active site | 283 | 1 | Phosphocysteine intermediate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 271 | 1 | Phosphothreonine Ref.11 | ||||||
| Modified residue | 467 | 1 | Phosphoserine Ref.10 Ref.12 | ||||||
Experimental info | |||||||||
| Sequence conflict | 118 | 1 | A → P in BAA09533. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "CDC14 of Saccharomyces cerevisiae. Cloning, sequence analysis, and transcription during the cell cycle." Wan J., Xu H., Grunstein M. J. Biol. Chem. 267:11274-11280(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Dominant mutant alleles of yeast protein kinase gene CDC15 suppress the lte1 defect in termination of M phase and genetically interact with CDC14." Shirayama M., Matsui Y., Toh-e A. Mol. Gen. Genet. 251:176-185(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. |
| [3] | "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [4] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [5] | "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y. Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [6] | Mai B., Lipp M. Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-421. |
| [7] | "Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit." Shou W., Sakamoto K.M., Keener J., Morimoto K.W., Traverso E.E., Azzam R., Hoppe G.J., Feldman R.M.R., DeModena J., Moazed D., Charbonneau H., Nomura M., Deshaies R.J. Mol. Cell 8:45-55(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT. |
| [8] | "The Cdc14 phosphatase and the FEAR network control meiotic spindle disassembly and chromosome segregation." Marston A.L., Lee B.H., Amon A. Dev. Cell 4:711-726(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| [9] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [10] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-467, MASS SPECTROMETRY. Strain: ADR376. |
| [11] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-271, MASS SPECTROMETRY. |
| [12] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-467, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M61194 Genomic DNA. Translation: AAA34477.1. Frameshift. D55715 Genomic DNA. Translation: BAA09533.1. D50617 Genomic DNA. Translation: BAA09267.1. X75077 Genomic DNA. Translation: CAA52971.1. Frameshift. BK006940 Genomic DNA. Translation: DAA12468.1. |
| PIR | S56283. |
| RefSeq | NP_116684.3. NM_001179993.3. |
3D structure databases | |
| ProteinModelPortal | Q00684. |
| SMR | Q00684. Positions 5-336. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-5116N. |
| IntAct | Q00684. 160 interactions. |
| MINT | MINT-564685. |
Proteomic databases | |
| PaxDb | Q00684. |
| PeptideAtlas | Q00684. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YFR028C; YFR028C; YFR028C. |
| GeneID | 850585. |
| KEGG | sce:YFR028C. |
Organism-specific databases | |
| CYGD | YFR028c. |
| SGD | S000001924. CDC14. |
Phylogenomic databases | |
| eggNOG | COG2453. |
| GeneTree | ENSGT00390000010254. |
| HOGENOM | HOG000198341. |
| KO | K06639. |
| OMA | ANECIGF. |
| OrthoDB | EOG4MD17S. |
Enzyme and pathway databases | |
| BioCyc | YEAST:G3O-30477-MONOMER. |
| SABIO-RK | Q00684. |
Gene expression databases | |
| Genevestigator | Q00684. |
| GermOnline | YFR028C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR026070. CDC14. IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view] |
| PANTHER | PTHR23339:SF22. PTHR23339:SF22. 1 hit. |
| Pfam | PF00782. DSPc. 1 hit. [Graphical view] |
| SMART | SM00195. DSPc. 1 hit. [Graphical view] |
| PROSITE | PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 966423. |
Entry information
| Entry name | CDC14_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q00684 Secondary accession number(s): D6VTQ8, Q05180, Q05673 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VI Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with
