ID   NDUS3_CANMA             Reviewed;         276 AA.
AC   Q00673;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   13-SEP-2023, entry version 98.
DE   RecName: Full=Probable NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrial;
DE            EC=7.1.1.2;
DE   AltName: Full=Alkane-inducible protein 1;
DE   AltName: Full=CI-31kD;
DE   AltName: Full=Complex I-30kD;
DE   Flags: Precursor;
GN   Name=ALI1;
OS   Candida maltosa (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=5479;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 28140 / CBS 5611 / IAM 12247 / JCM 1504 / NBRC 1977;
RX   PubMed=1937042; DOI=10.1016/0378-1119(91)90566-t;
RA   Hwang C.W., Yano K., Takagi M.;
RT   "Sequences of two tandem genes regulated by carbon sources, one being
RT   essential for n-alkane assimilation in Candida maltosa.";
RL   Gene 106:61-69(1991).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). Essential for N-alkane assimilation. {ECO:0000250,
CC       ECO:0000269|PubMed:1937042}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBUNIT: Complex I is composed of about 30 different subunits. This is
CC       a component of the iron-sulfur protein fraction.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane.
CC   -!- SIMILARITY: Belongs to the complex I 30 kDa subunit family.
CC       {ECO:0000305}.
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DR   EMBL; M61102; AAA34360.1; -; Genomic_DNA.
DR   PIR; JT0591; JT0591.
DR   AlphaFoldDB; Q00673; -.
DR   SMR; Q00673; -.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.30.460.80; NADH:ubiquinone oxidoreductase, 30kDa subunit; 1.
DR   HAMAP; MF_01357; NDH1_NuoC; 1.
DR   InterPro; IPR010218; NADH_DH_suC.
DR   InterPro; IPR037232; NADH_quin_OxRdtase_su_C/D-like.
DR   InterPro; IPR001268; NADH_UbQ_OxRdtase_30kDa_su.
DR   InterPro; IPR020396; NADH_UbQ_OxRdtase_CS.
DR   NCBIfam; TIGR01961; NuoC_fam; 1.
DR   PANTHER; PTHR10884:SF14; NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR10884; NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3; 1.
DR   Pfam; PF00329; Complex1_30kDa; 1.
DR   SUPFAM; SSF143243; Nqo5-like; 1.
DR   PROSITE; PS00542; COMPLEX1_30K; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Oxidoreductase; Respiratory chain; Transit peptide; Translocase;
KW   Transport; Ubiquinone.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..276
FT                   /note="Probable NADH-ubiquinone oxidoreductase 30.4 kDa
FT                   subunit, mitochondrial"
FT                   /id="PRO_0000020001"
FT   REGION          248..276
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   276 AA;  32158 MW;  1CA55A6CF1459A72 CRC64;
     MISRTLLKRT VPASRLLRSF TTSNVRLSAH EEDLVNVNNL PRPKPTENYV PLINPTEKYK
     VQIEELHKFG TYIMSCLPKY IQQFSVWKDE LTIYVAPSAI RPVMSYLKNH TSCQFKAVMD
     ITAADYPSRT NRFDVVYNLL SDRHNSRIRV KTYANETSPV PSVTPLFNGA NWYERETYDL
     FGVFFVGHPD LRRIMTDYGF EGHPLRKDFP TTGYTEVRYD EEKKRVIYEP LELTQAWRNF
     TVGSSVWEPV GEGKDFTPES FKLPTPQPEP EQEEKK
//