Reviewed, UniProtKB/Swiss-Prot Q00673 (NDUS3_CANMA)

Names and origin

Protein names

Recommended name:
    Probable NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    Complex I-30kD
    CI-31kD
    Alkane-inducible protein 1

Gene names

Name:

ALI1

Organism

Candida maltosa (Yeast)

Taxonomic identifier

5479 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential for N-alkane assimilation. Ref.2 Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Subunit structure	Complex I is composed of about 30 different subunits. This is a component of the iron-sulfur protein fraction.
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the complex I 30 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 276		Probable NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrial		PRO_0000020001

Sequences

	Sequence		Length	Mass (Da)
	Q00673-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 1CA55A6CF1459A72	FASTA	276	32,158
	10 20 30 40 50 60 MISRTLLKRT VPASRLLRSF TTSNVRLSAH EEDLVNVNNL PRPKPTENYV PLINPTEKYK 70 80 90 100 110 120 VQIEELHKFG TYIMSCLPKY IQQFSVWKDE LTIYVAPSAI RPVMSYLKNH TSCQFKAVMD 130 140 150 160 170 180 ITAADYPSRT NRFDVVYNLL SDRHNSRIRV KTYANETSPV PSVTPLFNGA NWYERETYDL 190 200 210 220 230 240 FGVFFVGHPD LRRIMTDYGF EGHPLRKDFP TTGYTEVRYD EEKKRVIYEP LELTQAWRNF 250 260 270 TVGSSVWEPV GEGKDFTPES FKLPTPQPEP EQEEKK

References

[1]	"Sequences of two tandem genes regulated by carbon sources, one being essential for n-alkane assimilation in Candida maltosa." Hwang C.W., Yano K., Takagi M. Gene 106:61-69(1991) [PubMed: 1937042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Bairoch A. Unpublished observations (OCT-1993) Cited for: POSSIBLE FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M61102 Genomic DNA. Translation: AAA34360.1.
PIR	JT0591.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.6.5.3. 3846. 1.6.99.3. 3846.
Family and domain databases
InterPro	IPR010218. NADH_DH_csu. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS3_CANMA

Accession

Primary (citable) accession number: Q00673

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)