Reviewed, UniProtKB/Swiss-Prot Q00667 (HGD_EMENI)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisic acid oxidase
    Homogentisate oxygenase
    Homogentisicase

Gene names

Name:	hmgA
ORF Names:	AN1897

Organism

Emericella nidulans (Aspergillus nidulans)

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Homogentisate + O(2) = 4-maleylacetoacetate.
Cofactor	Iron.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
Induction	During growth with phenylacetate and phenylalanine.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Homogentisate 1,2-dioxygenase		PRO_0000220245
Sites
	Metal binding	340	1	Iron By similarity
	Metal binding	346	1	Iron By similarity
	Metal binding	377	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00667-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4AE6414823A04C0D	FASTA	448	50,168
	10 20 30 40 50 60 MPVTEFSFKD PYTYQNGFDS YHESEAIEGA LPVGHNSPQK APYGLYAEKL SGTAFTAPRH 70 80 90 100 110 120 ENKQTWVYRI LPAAAHENFV EEDASSYHTL SDAKKLQHIP NQLRWDPFDL DETVDWVHGL 130 140 150 160 170 180 HLVAGSGDPT VKQGLGILLY AAGKDMGKEA FYSADGDFLI VAQHGVLDIQ TELGRLLVRP 190 200 210 220 230 240 NEICVIPRGV RYRVTLPDGP VRGYICELYQ GHYQLPELGP IGSNGLANAR DFQAPVAAFD 250 260 270 280 290 300 DEEGPTEYRL YSKFNNHLFS ARQDHTPFDI VAWHGNYYPY KYDLGRFNTM GSVSFDHPDP 310 320 330 340 350 360 SIYTVLTGPS DHVGTAIADF VIFPPRWLVA EKTFRPPWYH RNTMSEFMGL ITGNYDAKTG 370 380 390 400 410 420 GGFQPAGASL HNIMSAHGPD MHAFEGASNA DLKPTKIGDG SMAFMFESSL MVGVSEWGLK 430 440 TCQKVQEEYN EHSWQPLKRH FKDPRKAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a gene encoding a homogentisate dioxygenase from Aspergillus nidulans and identification of its human and plant homologues." Fernandez-Canon J.M., Penalva M.A. J. Biol. Chem. 270:21199-21205(1995) [PubMed: 7673153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: biA1.
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U30797 Genomic DNA. Translation: AAC49071.1. AJ001836 Genomic DNA. Translation: CAA05042.1. AACD01000029 Genomic DNA. Translation: EAA65062.1.
PIR	A57435.
RefSeq	XP_659501.1.
3D structure databases
HSSP	HSSP built from PDB template 1EYB based on UniProtKB Q93099.
ModBase	Search...
Genome annotation databases
GeneID	2874820.
KEGG	ani:AN1897.2.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-12040.
Family and domain databases
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_EMENI

Accession

Primary (citable) accession number: Q00667
Secondary accession number(s): Q5BC33

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)