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Protein

Putative beta-galactosidase

Gene

CARSR12

Organism
Dianthus caryophyllus (Carnation) (Clove pink)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei187 – 1871Proton donorSequence Analysis
Active sitei257 – 2571NucleophileSequence Analysis

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative beta-galactosidase (EC:3.2.1.23)
Short name:
Lactase
Alternative name(s):
SR12 protein
Gene namesi
Name:CARSR12
OrganismiDianthus caryophyllus (Carnation) (Clove pink)
Taxonomic identifieri3570 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesCaryophyllaceaeDianthus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Chaini30 – 731702Putative beta-galactosidasePRO_0000012196Add
BLAST

Expressioni

Tissue specificityi

Senescing flower petals.

Inductioni

By ethylene.

Structurei

3D structure databases

ProteinModelPortaliQ00662.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00662-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLCGKENNVM KMMLVYVFVL ITLISCVYGN VWYDYRAIKI NDQRRILLSG
60 70 80 90 100
SIHYPRSTPE MWPDIIEKAK DSQLDVIQTY VFWNGHEPSE GKYYFEGRYD
110 120 130 140 150
LVKFIKLIHQ AGLFVHLRIG PFACAEWNFG GFPVWLKYVP GIEFRTDNGP
160 170 180 190 200
FKEKMQVFTT KIVDMMKAEK LFHWQGGPII LNQIENEYGP VEWEIGAPGK
210 220 230 240 250
AYTHWAAQMA QSLNAGVPWI MCKQDSDVPD NVIDTCNGFY CEGFVPKDKS
260 270 280 290 300
KPKMWTENWT GWYTEYGKPV PYRPAEDVAF SVARFIQNGG SFMNYYMFHG
310 320 330 340 350
GTNFETTAGR FVSTSYDYDA PLDEYGLPRE PKYTHLKNLH KAIKMCEPAL
360 370 380 390 400
VSSDAKVTNL GSNQEAHVYS SNSGSCAAFL ANYDPKWSVK VTFSGMEFEL
410 420 430 440 450
PAWSISILPD CKKEVYNTAR VNEPSPKLHS KMTPVISNLN WQSYSDEVPT
460 470 480 490 500
ADSPGTFREK KLYEQINMTW DKSDYLWYMT DVVLDGNEGF LKKGDEPWLT
510 520 530 540 550
VNSAGHVLHV FVNGQLQGHA YGSLAKPQLT FSQKVKMTAG VNRISLLSAV
560 570 580 590 600
VGLANVGWHF ERYNQGVLGP VTLSGLNEGT RDLTWQYWSY KIGTKGEEQQ
610 620 630 640 650
VYNSGGSSHV QWGPPAWKQP LVWYKTTFDA PGGNDPLALD LGSMGKGQAW
660 670 680 690 700
INGQSIGRHW SNNIAKGSCN DNCNYAGTYT ETKCLSDCGK SSQKWYHVPR
710 720 730
SWLQPRGNLL VVFEEWGGDT KWVSLVKRTI A
Length:731
Mass (Da):82,864
Last modified:April 1, 1993 - v1
Checksum:i83FA8B5A3779C051
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57171 Genomic DNA. Translation: CAA40459.1.
PIRiS16595.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57171 Genomic DNA. Translation: CAA40459.1.
PIRiS16595.

3D structure databases

ProteinModelPortaliQ00662.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of an ethylene-regulated flower senescence-related gene from carnation."
    Raghothama K.G., Lawton K.A., Goldsbrough P.B., Woodson W.R.
    Plant Mol. Biol. 17:61-71(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. White Sim.
    Tissue: Petal.

Entry informationi

Entry nameiBGAL_DIACA
AccessioniPrimary (citable) accession number: Q00662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 1, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.