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Q00662

- BGAL_DIACA

UniProt

Q00662 - BGAL_DIACA

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Protein
Putative beta-galactosidase
Gene
CARSR12
Organism
Dianthus caryophyllus (Carnation) (Clove pink)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei187 – 1871Proton donor Reviewed prediction
Active sitei257 – 2571Nucleophile Reviewed prediction

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative beta-galactosidase (EC:3.2.1.23)
Short name:
Lactase
Alternative name(s):
SR12 protein
Gene namesi
Name:CARSR12
OrganismiDianthus caryophyllus (Carnation) (Clove pink)
Taxonomic identifieri3570 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesCaryophyllaceaeDianthus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed prediction
Add
BLAST
Chaini30 – 731702Putative beta-galactosidase
PRO_0000012196Add
BLAST

Expressioni

Tissue specificityi

Senescing flower petals.

Inductioni

By ethylene.

Structurei

3D structure databases

ProteinModelPortaliQ00662.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00662-1 [UniParc]FASTAAdd to Basket

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MLCGKENNVM KMMLVYVFVL ITLISCVYGN VWYDYRAIKI NDQRRILLSG    50
SIHYPRSTPE MWPDIIEKAK DSQLDVIQTY VFWNGHEPSE GKYYFEGRYD 100
LVKFIKLIHQ AGLFVHLRIG PFACAEWNFG GFPVWLKYVP GIEFRTDNGP 150
FKEKMQVFTT KIVDMMKAEK LFHWQGGPII LNQIENEYGP VEWEIGAPGK 200
AYTHWAAQMA QSLNAGVPWI MCKQDSDVPD NVIDTCNGFY CEGFVPKDKS 250
KPKMWTENWT GWYTEYGKPV PYRPAEDVAF SVARFIQNGG SFMNYYMFHG 300
GTNFETTAGR FVSTSYDYDA PLDEYGLPRE PKYTHLKNLH KAIKMCEPAL 350
VSSDAKVTNL GSNQEAHVYS SNSGSCAAFL ANYDPKWSVK VTFSGMEFEL 400
PAWSISILPD CKKEVYNTAR VNEPSPKLHS KMTPVISNLN WQSYSDEVPT 450
ADSPGTFREK KLYEQINMTW DKSDYLWYMT DVVLDGNEGF LKKGDEPWLT 500
VNSAGHVLHV FVNGQLQGHA YGSLAKPQLT FSQKVKMTAG VNRISLLSAV 550
VGLANVGWHF ERYNQGVLGP VTLSGLNEGT RDLTWQYWSY KIGTKGEEQQ 600
VYNSGGSSHV QWGPPAWKQP LVWYKTTFDA PGGNDPLALD LGSMGKGQAW 650
INGQSIGRHW SNNIAKGSCN DNCNYAGTYT ETKCLSDCGK SSQKWYHVPR 700
SWLQPRGNLL VVFEEWGGDT KWVSLVKRTI A 731
Length:731
Mass (Da):82,864
Last modified:April 1, 1993 - v1
Checksum:i83FA8B5A3779C051
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57171 Genomic DNA. Translation: CAA40459.1.
PIRiS16595.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57171 Genomic DNA. Translation: CAA40459.1 .
PIRi S16595.

3D structure databases

ProteinModelPortali Q00662.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SUPFAMi SSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of an ethylene-regulated flower senescence-related gene from carnation."
    Raghothama K.G., Lawton K.A., Goldsbrough P.B., Woodson W.R.
    Plant Mol. Biol. 17:61-71(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. White Sim.
    Tissue: Petal.

Entry informationi

Entry nameiBGAL_DIACA
AccessioniPrimary (citable) accession number: Q00662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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