Q00662 (BGAL_DIACA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative beta-galactosidase
Short name=Lactase
EC=3.2.1.23

Alternative name(s):
SR12 protein

Gene names

Name:

CARSR12

Organism

Dianthus caryophyllus (Carnation) (Clove pink)

Taxonomic identifier

3570 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Dianthus

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Tissue specificity	Senescing flower petals.
Induction	By ethylene.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 731	702	Putative beta-galactosidase		PRO_0000012196
Sites
	Active site	187	1	Proton donor Potential
	Active site	257	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	Q00662 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 83FA8B5A3779C051	FASTA	731	82,864
	10 20 30 40 50 60 MLCGKENNVM KMMLVYVFVL ITLISCVYGN VWYDYRAIKI NDQRRILLSG SIHYPRSTPE 70 80 90 100 110 120 MWPDIIEKAK DSQLDVIQTY VFWNGHEPSE GKYYFEGRYD LVKFIKLIHQ AGLFVHLRIG 130 140 150 160 170 180 PFACAEWNFG GFPVWLKYVP GIEFRTDNGP FKEKMQVFTT KIVDMMKAEK LFHWQGGPII 190 200 210 220 230 240 LNQIENEYGP VEWEIGAPGK AYTHWAAQMA QSLNAGVPWI MCKQDSDVPD NVIDTCNGFY 250 260 270 280 290 300 CEGFVPKDKS KPKMWTENWT GWYTEYGKPV PYRPAEDVAF SVARFIQNGG SFMNYYMFHG 310 320 330 340 350 360 GTNFETTAGR FVSTSYDYDA PLDEYGLPRE PKYTHLKNLH KAIKMCEPAL VSSDAKVTNL 370 380 390 400 410 420 GSNQEAHVYS SNSGSCAAFL ANYDPKWSVK VTFSGMEFEL PAWSISILPD CKKEVYNTAR 430 440 450 460 470 480 VNEPSPKLHS KMTPVISNLN WQSYSDEVPT ADSPGTFREK KLYEQINMTW DKSDYLWYMT 490 500 510 520 530 540 DVVLDGNEGF LKKGDEPWLT VNSAGHVLHV FVNGQLQGHA YGSLAKPQLT FSQKVKMTAG 550 560 570 580 590 600 VNRISLLSAV VGLANVGWHF ERYNQGVLGP VTLSGLNEGT RDLTWQYWSY KIGTKGEEQQ 610 620 630 640 650 660 VYNSGGSSHV QWGPPAWKQP LVWYKTTFDA PGGNDPLALD LGSMGKGQAW INGQSIGRHW 670 680 690 700 710 720 SNNIAKGSCN DNCNYAGTYT ETKCLSDCGK SSQKWYHVPR SWLQPRGNLL VVFEEWGGDT 730 KWVSLVKRTI A

References

[1]	"Characterization of an ethylene-regulated flower senescence-related gene from carnation." Raghothama K.G., Lawton K.A., Goldsbrough P.B., Woodson W.R. Plant Mol. Biol. 17:61-71(1991) [PubMed: 1868223] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. White Sim. Tissue: Petal.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57171 Genomic DNA. Translation: CAA40459.1.
PIR	S16595.
3D structure databases
ProteinModelPortal	Q00662.
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_subgr_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_DIACA

Accession

Primary (citable) accession number: Q00662

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 31, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program