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Protein

Chondroitin sulfate proteoglycan 4

Gene

Cspg4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.7 Publications

GO - Molecular functioni

  • collagen binding Source: UniProtKB
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  • angiogenesis Source: UniProtKB-KW
  • cell migration Source: GO_Central
  • inflammatory response Source: RGD
  • negative regulation of neuron projection development Source: RGD
  • neuron remodeling Source: RGD
  • response to lithium ion Source: RGD
  • tissue remodeling Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Transducer

Keywords - Biological processi

Angiogenesis, Differentiation, Tissue remodeling

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate proteoglycan 4
Alternative name(s):
Chondroitin sulfate proteoglycan NG2
HSN tumor-specific antigen
Gene namesi
Name:Cspg4
Synonyms:Ng2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619942. Cspg4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 22252196Extracellular1 PublicationAdd
BLAST
Transmembranei2226 – 225025HelicalSequence analysisAdd
BLAST
Topological domaini2251 – 232676Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB-SubCell
  • cell surface Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: RGD
  • lamellipodium membrane Source: UniProtKB-SubCell
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi999 – 9991S → A: No chondroitin sulfate attachment. Loss of localization to the retraction fibers. 1 Publication
Mutagenesisi1342 – 13421S → A: No effect on chondroitin sulfate attachment. 1 Publication
Mutagenesisi2256 – 22561T → E: Localized to the lamellipodia. Increases cell motility independently of PRKCA activation. 1 Publication
Mutagenesisi2256 – 22561T → V: Apically localized. Loss of PRKCA-dependent cell motility. 1 Publication
Mutagenesisi2265 – 22651T → E: Behaves as wild-type. 1 Publication
Mutagenesisi2265 – 22651T → V: Behaves as wild-type. 1 Publication
Mutagenesisi2278 – 22781T → E: Behaves as wild-type. 1 Publication
Mutagenesisi2278 – 22781T → V: Behaves as wild-type. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29291 PublicationAdd
BLAST
Chaini30 – 23262297Chondroitin sulfate proteoglycan 4PRO_0000026695Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence analysis
Disulfide bondi170 ↔ 193PROSITE-ProRule annotation
Glycosylationi349 – 3491N-linked (GlcNAc...)Sequence analysis
Disulfide bondi355 ↔ 381PROSITE-ProRule annotation
Glycosylationi428 – 4281N-linked (GlcNAc...)Sequence analysis
Glycosylationi686 – 6861N-linked (GlcNAc...)Sequence analysis
Glycosylationi773 – 7731N-linked (GlcNAc...)Sequence analysis
Glycosylationi999 – 9991O-linked (Xyl...) (chondroitin sulfate)1 Publication
Glycosylationi1135 – 11351N-linked (GlcNAc...)Sequence analysis
Glycosylationi1206 – 12061N-linked (GlcNAc...)Sequence analysis
Glycosylationi1368 – 13681N-linked (GlcNAc...)Sequence analysis
Glycosylationi1453 – 14531N-linked (GlcNAc...)Sequence analysis
Glycosylationi1649 – 16491N-linked (GlcNAc...)Sequence analysis
Glycosylationi1913 – 19131N-linked (GlcNAc...)Sequence analysis
Glycosylationi2020 – 20201N-linked (GlcNAc...)Sequence analysis
Glycosylationi2038 – 20381N-linked (GlcNAc...)Sequence analysis
Glycosylationi2044 – 20441N-linked (GlcNAc...)Sequence analysis
Glycosylationi2079 – 20791N-linked (GlcNAc...)Sequence analysis
Modified residuei2256 – 22561Phosphothreonine; by PKC/PRKCA1 Publication

Post-translational modificationi

N-glycosylated.
O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation. Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan

Proteomic databases

PaxDbiQ00657.
PRIDEiQ00657.

PTM databases

iPTMnetiQ00657.
PhosphoSiteiQ00657.

Expressioni

Tissue specificityi

Neural cells and also extraneural tissues, especially in the developing mesenchyme.

Developmental stagei

The level of expression is highest on immature, proliferating cells and decreases as these cells differentiate.

Interactioni

Subunit structurei

Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16 (By similarity). Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA.By similarity10 Publications

GO - Molecular functioni

  • collagen binding Source: UniProtKB

Protein-protein interaction databases

BioGridi249551. 1 interaction.
MINTiMINT-143877.
STRINGi10116.ENSRNOP00000023326.

Structurei

3D structure databases

ProteinModelPortaliQ00657.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 193164Laminin G-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini203 – 381179Laminin G-like 2PROSITE-ProRule annotationAdd
BLAST
Repeati431 – 52494CSPG 11 PublicationAdd
BLAST
Repeati557 – 64488CSPG 21 PublicationAdd
BLAST
Repeati666 – 765100CSPG 31 PublicationAdd
BLAST
Repeati793 – 88290CSPG 41 PublicationAdd
BLAST
Repeati904 – 99390CSPG 51 PublicationAdd
BLAST
Repeati1024 – 111289CSPG 61 PublicationAdd
BLAST
Repeati1132 – 122089CSPG 71 PublicationAdd
BLAST
Repeati1245 – 134197CSPG 81 PublicationAdd
BLAST
Repeati1364 – 145390CSPG 91 PublicationAdd
BLAST
Repeati1480 – 156586CSPG 101 PublicationAdd
BLAST
Repeati1587 – 168397CSPG 111 PublicationAdd
BLAST
Repeati1711 – 180797CSPG 121 PublicationAdd
BLAST
Repeati1839 – 192688CSPG 131 PublicationAdd
BLAST
Repeati1948 – 203386CSPG 141 PublicationAdd
BLAST
Repeati2052 – 2151100CSPG 151 PublicationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni30 – 640611Globular or compact configuration stabilized by disulfide bondsAdd
BLAST
Regioni30 – 640611Neurite growth inhibitionAdd
BLAST
Regioni575 – 1044470Interaction with COL6A2Add
BLAST
Regioni632 – 1450819Interaction with COL5A1Add
BLAST
Regioni1590 – 2225636Neurite growth inhibitionAdd
BLAST
Regioni1591 – 2225635Cysteine-containingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi2324 – 23263PDZ-binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi640 – 1590951Gly/Ser-rich (glycosaminoglycan attachment domain)Add
BLAST

Sequence similaritiesi

Contains 15 CSPG (NG2) repeats.Curated
Contains 2 laminin G-like domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3597. Eukaryota.
ENOG410XQ29. LUCA.
HOGENOMiHOG000170195.
HOVERGENiHBG081360.
InParanoidiQ00657.
KOiK08115.
PhylomeDBiQ00657.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001791. Laminin_G.
[Graphical view]
PfamiPF00054. Laminin_G_1. 1 hit.
PF02210. Laminin_G_2. 1 hit.
[Graphical view]
SMARTiSM00282. LamG. 2 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS50025. LAM_G_DOMAIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00657-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLSPGHPLS APALALILTL ALLVRSTAPA SFFGENHLEV PVPSALTRVD
60 70 80 90 100
LLLQFSTSQP EALLLLAAGQ TDHLLLQLQS GHLQVRLALG QNELSLQTPA
110 120 130 140 150
DTVLSDSTTH TVVLTVSNSW AVLSVDGVLN TSAPIPKASH LKVPYGLFVG
160 170 180 190 200
SSGSLDLPYL KGISRPLRGC LHSAILNGRN LLRPLTPDVH EGCAEEFSAG
210 220 230 240 250
DEVGLGFSGP HSLAAFPAWS TREEGTLEFT LTTRSQQAPL AFQAGDKRGN
260 270 280 290 300
FIYVDIFEGH LRAVVEKGQG TMLLRNSVPV ADGQPHEVSV HIDVHRLEIS
310 320 330 340 350
VDQYPTRTFN RGVLSYLEPR GSLLLGGLDT EASRHLQEHR LGLTPGAANI
360 370 380 390 400
SLVGCIEDFS VNGRRLGLRD AWLTRDMAAG CRPEEDEYEE EVYGPFEAFS
410 420 430 440 450
TLAPEAWPVM DLPEPCVPEP GLPAVFANFT QLLTISPLVV AEGGTAWLEW
460 470 480 490 500
RHVQPTLDLT EAELRKSQVL FSVSQGARHG ELELDIPGAQ TRKMFTLLDV
510 520 530 540 550
VNRKARFVHD GSEDTSDQLM LEVSVTSRAP VPSCLRRGQI YILPIQVNPV
560 570 580 590 600
NDPPRIVFPH GSLMVILEHT QKPLGPEIFQ AYDPDSACEG LTIQLLGVSA
610 620 630 640 650
SVPVEHRDQP GEPVTEFSCR DLEAGNIVYV HRGGPAQDLT FRVSDGMQAS
660 670 680 690 700
GPATLKVVAV RPAIQILHNT GLRLAQGSAA AILPANLSVE TNAVGQDVSV
710 720 730 740 750
LFRVTGTLQF GELQKQGAGG VEGTEWWDTL AFHQRDVEQG RVRYLSTDPQ
760 770 780 790 800
HHTQDTVEDL TLEVQVGQET LSNLSFPVTI QRATVWMLQL EPLHTQNPHQ
810 820 830 840 850
ETLTSAHLEA SLEEEGEGGP YPHIFHYELV QAPRRGNLLL QGTRLSDGQS
860 870 880 890 900
FSQSDLQAGR VTYRATTRTS EAAEDSFRFR VTSPPHFSPL YTFPIHIGGD
910 920 930 940 950
PNAPVLTNVL LMVPEGGEGV LSADHLFVKS LNSASYLYEV MEQPHHGSLA
960 970 980 990 1000
WRDPKGRATP VTSFTNEDLL HGRLVYQHDD SETIEDDIPF VATRQGEGSG
1010 1020 1030 1040 1050
DMAWEEVRGV FRVAIQPVND HAPVQTISRV FHVARGGQRL LTTDDVAFSD
1060 1070 1080 1090 1100
ADSGFSDAQL VLTRKDLLFG SIVAMEEPTR PIYRFTQEDL RKKQVLFVHS
1110 1120 1130 1140 1150
GADHGWLQLQ VSDGQHQATA MLEVQASEPY LHVANSSSLV VPQGGQGTID
1160 1170 1180 1190 1200
TAVLHLDTNL DIRSGNEVHY HVTAGPHWGQ LLRDGQSVTS FSQRDLLDGA
1210 1220 1230 1240 1250
ILYSHNGSLS PQDTLALSVA AGPVHTSTVL QVTIALEGPL APLQLVQHKR
1260 1270 1280 1290 1300
IYVFQGEAAE IRRDQLEVVQ EAVLPADIMF SLRSPPNAGY LVMVSHGASA
1310 1320 1330 1340 1350
DGPPSLDPVQ RFSQEAINSG RVLYLHSRPG AWSDSFSLDV ASGLGDPLEG
1360 1370 1380 1390 1400
ISVELEVLPT VIPLDVQNFS VPEGGTRTLA PPLIQITGPY LGTLPGLVLQ
1410 1420 1430 1440 1450
VLEPPQHGAL QKEDRPQDGT LSTFSWREVE EQLIRYVHDG SETQTDGFIL
1460 1470 1480 1490 1500
LANASEMDRQ SQPMAFTITI LPVNDQPPVI TTNTGLQIWE GAIVPIPPEA
1510 1520 1530 1540 1550
LRGIDSDSGP EDLVYTIEQP SNGRIALRVA PDAEAHRFTQ AQLDSGLVLF
1560 1570 1580 1590 1600
SHRGALEGGF HFDLSDGVHT SPGHFFRVVA QKQVLLSLEG SRKLTVCPES
1610 1620 1630 1640 1650
VQPLSSQSLS ASSSTGSDPR HLLYQVVRGP QLGRLLHAQQ GSAEEALVNF
1660 1670 1680 1690 1700
TQAEVNAGNI LYEHEISSEP FWEAHDTIGL LLSSSPARDL AATLAVTVSF
1710 1720 1730 1740 1750
DAACPQRPSR LWRNKGLWVP EGQRAKITVA ALDAANLLAS VPASQRGRHD
1760 1770 1780 1790 1800
VLFQITQFPT RGQLLVSEEP LHARRPHFLQ SELTAGQLVY AHGGGGTQQD
1810 1820 1830 1840 1850
GFRFRAHLQG PTGASVAGPQ TSEAFVITVR DVNERPPQPQ ASIPLRITRG
1860 1870 1880 1890 1900
SRAPVSRAQL SVVDPDSAPG EIEYEVQRAP HNGFLSLAGD NTGPVTHFTQ
1910 1920 1930 1940 1950
ADVDAGRLAF VANGSSVAGV FQLSMSDGAS PPIPMSLAVD VLPSTIEVQL
1960 1970 1980 1990 2000
RAPLEVPQAL GRSSLSRQQL QVISDREEPD VAYRLTQGPL YGQVLVGGQP
2010 2020 2030 2040 2050
ASAFSQLQVD QGDVVFAFTN FSSSQDHFKV LALARGVNAS ATVNVTVQAL
2060 2070 2080 2090 2100
LHVWAGGPWP QGTTLRLDPT VLDASELANR TGSMPRFRLL EGPRYGRVVR
2110 2120 2130 2140 2150
VSQGRAESRT NQLVEDFTQQ DLEEGRLGLE VGRPEGRSTG PTGDRLTLEL
2160 2170 2180 2190 2200
QATGVPPAVA LLDFATEPYH AAKFYKVTLL SVPEAARTET EKTGKSTPTG
2210 2220 2230 2240 2250
QPGQAASSPM PTVAKSGFLG FLEANMFSVI IPVCLVLLLL ALILPLLFYL
2260 2270 2280 2290 2300
RKRNKTGKHD VQVLTAKPRN GLAGDTETFR KVEPGQAIPL TTVPGQGPPP
2310 2320
GGQPDPELLQ FCRTPNPALR NGQYWV
Length:2,326
Mass (Da):251,909
Last modified:May 30, 2000 - v2
Checksum:iFA3E3BCEDB7E5EF5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1391 – 13922LG → FP AA sequence (PubMed:8077056).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56541 mRNA. Translation: CAA39884.2.
PIRiS16025. A61208.
RefSeqiNP_112284.1. NM_031022.1.
UniGeneiRn.10381.

Genome annotation databases

GeneIDi81651.
KEGGirno:81651.
UCSCiRGD:619942. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56541 mRNA. Translation: CAA39884.2.
PIRiS16025. A61208.
RefSeqiNP_112284.1. NM_031022.1.
UniGeneiRn.10381.

3D structure databases

ProteinModelPortaliQ00657.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249551. 1 interaction.
MINTiMINT-143877.
STRINGi10116.ENSRNOP00000023326.

PTM databases

iPTMnetiQ00657.
PhosphoSiteiQ00657.

Proteomic databases

PaxDbiQ00657.
PRIDEiQ00657.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi81651.
KEGGirno:81651.
UCSCiRGD:619942. rat.

Organism-specific databases

CTDi1464.
RGDi619942. Cspg4.

Phylogenomic databases

eggNOGiKOG3597. Eukaryota.
ENOG410XQ29. LUCA.
HOGENOMiHOG000170195.
HOVERGENiHBG081360.
InParanoidiQ00657.
KOiK08115.
PhylomeDBiQ00657.

Miscellaneous databases

PROiQ00657.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001791. Laminin_G.
[Graphical view]
PfamiPF00054. Laminin_G_1. 1 hit.
PF02210. Laminin_G_2. 1 hit.
[Graphical view]
SMARTiSM00282. LamG. 2 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS50025. LAM_G_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSPG4_RAT
AccessioniPrimary (citable) accession number: Q00657
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: May 30, 2000
Last modified: July 6, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Valuable marker for several incompletely differentiated precursor cells.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.