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Q00651

- ITA4_MOUSE

UniProt

Q00651 - ITA4_MOUSE

Protein

Integrin alpha-4

Gene

Itga4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Integrins alpha-4/beta-1 (VLA-4 or LPAM-2) and alpha-4/beta-7 (LPAM-1) are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. Mice homozygous for a null mutation of the alpha-4 subunit gene die by day E11-E14 from detachment and rupture of the epicardium and coronary arteries.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei598 – 5992Cleavage

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi321 – 3299Sequence Analysis
    Calcium bindingi384 – 3929Sequence Analysis
    Calcium bindingi446 – 4549Sequence Analysis

    GO - Molecular functioni

    1. fibronectin binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. blood vessel remodeling Source: MGI
    2. cell adhesion Source: MGI
    3. cell-matrix adhesion Source: DFLAT
    4. cell migration Source: MGI
    5. chorio-allantoic fusion Source: MGI
    6. face development Source: MGI
    7. heart development Source: MGI
    8. heterophilic cell-cell adhesion Source: MGI
    9. integrin-mediated signaling pathway Source: UniProtKB-KW
    10. leukocyte cell-cell adhesion Source: MGI
    11. mesenchyme development Source: DFLAT
    12. negative regulation of protein homodimerization activity Source: UniProtKB
    13. single organismal cell-cell adhesion Source: DFLAT
    14. T cell migration Source: MGI
    15. visceral serous pericardium development Source: DFLAT

    Keywords - Molecular functioni

    Integrin, Receptor

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Integrin alpha-4
    Alternative name(s):
    CD49 antigen-like family member D
    Integrin alpha-IV
    Lymphocyte Peyer patch adhesion molecules subunit alpha
    Short name:
    LPAM subunit alpha
    VLA-4 subunit alpha
    CD_antigen: CD49d
    Gene namesi
    Name:Itga4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:96603. Itga4.

    Subcellular locationi

    GO - Cellular componenti

    1. cell-cell junction Source: MGI
    2. cell surface Source: DFLAT
    3. external side of plasma membrane Source: MGI
    4. integrin complex Source: InterPro

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4040Sequence AnalysisAdd
    BLAST
    Chaini41 – 1039999Integrin alpha-4PRO_0000016245Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi86 – 861N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi98 ↔ 108By similarity
    Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi151 ↔ 172By similarity
    Disulfide bondi190 ↔ 205By similarity
    Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi487 – 4871N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi493 ↔ 502By similarity
    Disulfide bondi508 ↔ 564By similarity
    Glycosylationi525 – 5251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi545 – 5451N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi629 ↔ 634By similarity
    Glycosylationi633 – 6331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi652 – 6521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi667 – 6671N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi705 ↔ 718By similarity
    Glycosylationi813 – 8131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi828 – 8281N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi859 ↔ 897By similarity
    Disulfide bondi904 ↔ 909By similarity
    Modified residuei1028 – 10281Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylation on Ser-1028 inhibits PXN binding.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ00651.
    PaxDbiQ00651.
    PRIDEiQ00651.

    PTM databases

    PhosphoSiteiQ00651.

    Expressioni

    Tissue specificityi

    Peyer patch homing cells.

    Gene expression databases

    CleanExiMM_ITGA4.
    GenevestigatoriQ00651.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Interacts with AMICA1; integrin alpha-4/beta-1 may regulate leukocyte to endothelial cells adhesion by controlling AMICA1 homodimerization By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ00651.
    SMRiQ00651. Positions 41-836, 983-1022.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini41 – 983943ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1008 – 103932CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei984 – 100724HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati42 – 10766FG-GAP 1Add
    BLAST
    Repeati117 – 18468FG-GAP 2Add
    BLAST
    Repeati193 – 24452FG-GAP 3Add
    BLAST
    Repeati245 – 30056FG-GAP 4Add
    BLAST
    Repeati301 – 35858FG-GAP 5Add
    BLAST
    Repeati362 – 41958FG-GAP 6Add
    BLAST
    Repeati423 – 48563FG-GAP 7Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi613 – 62311SG1Add
    BLAST
    Motifi1010 – 10145GFFKR motif

    Domaini

    The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.By similarity

    Sequence similaritiesi

    Belongs to the integrin alpha chain family.Curated
    Contains 7 FG-GAP repeats.Curated

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG324320.
    HOGENOMiHOG000088626.
    HOVERGENiHBG004538.
    InParanoidiQ00651.
    KOiK06483.
    PhylomeDBiQ00651.

    Family and domain databases

    InterProiIPR013519. Int_alpha_beta-p.
    IPR000413. Integrin_alpha.
    IPR013649. Integrin_alpha-2.
    IPR018184. Integrin_alpha_C_CS.
    [Graphical view]
    PfamiPF08441. Integrin_alpha2. 1 hit.
    [Graphical view]
    PRINTSiPR01185. INTEGRINA.
    SMARTiSM00191. Int_alpha. 6 hits.
    [Graphical view]
    PROSITEiPS51470. FG_GAP. 7 hits.
    PS00242. INTEGRIN_ALPHA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00651-1 [UniParc]FASTAAdd to Basket

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    MFSTKSAWLR NGGADQGPRG IALREAVMLL LYFGVPTGPS YNLDPENALL     50
    YQGPSGTLFG YSVVLHSHGS KRWLIVGAPT ASWLSNASVV NPGAIYRCGI 100
    RKNPNQTCEQ LQSGSPSGEP CGKTCLEERD NQWLGVTLSR QPGENGSIVT 150
    CGHRWKNIFY MKSDNKLPTG ICYVMPSDLR TELSKRMAPC YKDYTRKFGE 200
    NFASCQAGIS SFYTQDLIVM GAPGSSYWTG TVFVYNITTN QYKAFVDRQN 250
    QVKFGSYLGY SVGAGHFRSP HTTEVVGGAP QHEQIGKAYI FSIDENELNI 300
    VYEMKGKKLG SYFGASVCAV DLNADGFSDL LVGAPMQSTI REEGRVFVYI 350
    NSGMGAVMVE MERVLVGSDK YAARFGESIA NLGDIDNDGF EDIAIGAPQE 400
    DDLRGAVYIY NGRVDGISST YSQRIEGQQI SKSLRMFGQS ISGQIDADNN 450
    GYVDVAVGAF QSDSAVLLRT RPVVIVEASL SHPESVNRTK FDCTENGLPS 500
    VCMHLTLCFS YKGKEVPGYI VLFYNVSLDV HRKAESPSRF YFFSNGTSDV 550
    ITGSIRVSSS GEKCRTHQAF MRKDVRDILT PIHVEATYHL GHHVITKRNT 600
    EEFPPLQPIL QQKKEKDVIR KMINFARFCA YENCSADLQV SAKVGFLKPY 650
    ENKTYLAVGS MKTIMLNVSL FNAGDDAYET TLNVQLPTGL YFIKILDLEE 700
    KQINCEVTES SGIVKLACSL GYIYVDRLSR IDISFLLDVS SLSRAHEDLS 750
    ISVHASCENE GELDQVRDNR VTLTIPLRYE VMLTVHGLVN PTSFVYGSSE 800
    ENEPETCMAE KLNLTFHVIN TGISMAPNVS VKIMVPNSFL PQDDKLFNVL 850
    DVQTTTGQCH FKHYGRECTF AQQKGIAGTL TDIVKFLSKT DKRLLYCMKA 900
    DQHCLDFLCN FGKMESGKEA SVHIQLEGRP SILEMDETSS LKFEIKATAF 950
    PEPHPKVIEL NKDENVAHVF LEGLHHQRPK RHFTIIIITI SLLLGLIVLL 1000
    LISCVMWKAG FFKRQYKSIL QEENRRDSWS YVNSKSNDD 1039
    Length:1,039
    Mass (Da):115,696
    Last modified:April 1, 1993 - v1
    Checksum:iC8C0BC611F7E9847
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53176 mRNA. Translation: CAA37316.1.
    PIRiA41131.
    RefSeqiNP_034706.3. NM_010576.3.
    UniGeneiMm.31903.

    Genome annotation databases

    GeneIDi16401.
    KEGGimmu:16401.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53176 mRNA. Translation: CAA37316.1 .
    PIRi A41131.
    RefSeqi NP_034706.3. NM_010576.3.
    UniGenei Mm.31903.

    3D structure databases

    ProteinModelPortali Q00651.
    SMRi Q00651. Positions 41-836, 983-1022.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL2111481.

    PTM databases

    PhosphoSitei Q00651.

    Proteomic databases

    MaxQBi Q00651.
    PaxDbi Q00651.
    PRIDEi Q00651.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 16401.
    KEGGi mmu:16401.

    Organism-specific databases

    CTDi 3676.
    MGIi MGI:96603. Itga4.

    Phylogenomic databases

    eggNOGi NOG324320.
    HOGENOMi HOG000088626.
    HOVERGENi HBG004538.
    InParanoidi Q00651.
    KOi K06483.
    PhylomeDBi Q00651.

    Miscellaneous databases

    ChiTaRSi ITGA4. mouse.
    NextBioi 289557.
    PROi Q00651.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_ITGA4.
    Genevestigatori Q00651.

    Family and domain databases

    InterProi IPR013519. Int_alpha_beta-p.
    IPR000413. Integrin_alpha.
    IPR013649. Integrin_alpha-2.
    IPR018184. Integrin_alpha_C_CS.
    [Graphical view ]
    Pfami PF08441. Integrin_alpha2. 1 hit.
    [Graphical view ]
    PRINTSi PR01185. INTEGRINA.
    SMARTi SM00191. Int_alpha. 6 hits.
    [Graphical view ]
    PROSITEi PS51470. FG_GAP. 7 hits.
    PS00242. INTEGRIN_ALPHA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of cDNAs for the alpha subunit of the murine lymphocyte-Peyer's patch adhesion molecule."
      Neuhaus H., Hu M.C.-T., Hemler M.E., Takada Y., Holzmann B., Weissman I.L.
      J. Cell Biol. 115:1149-1158(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1028, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiITA4_MOUSE
    AccessioniPrimary (citable) accession number: Q00651
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3