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Q00651

- ITA4_MOUSE

UniProt

Q00651 - ITA4_MOUSE

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Protein

Integrin alpha-4

Gene

Itga4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Integrins alpha-4/beta-1 (VLA-4 or LPAM-2) and alpha-4/beta-7 (LPAM-1) are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. Mice homozygous for a null mutation of the alpha-4 subunit gene die by day E11-E14 from detachment and rupture of the epicardium and coronary arteries.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei598 – 5992Cleavage

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi321 – 3299Sequence Analysis
Calcium bindingi384 – 3929Sequence Analysis
Calcium bindingi446 – 4549Sequence Analysis

GO - Molecular functioni

  1. fibronectin binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. blood vessel remodeling Source: MGI
  2. cell adhesion Source: MGI
  3. cell-matrix adhesion Source: DFLAT
  4. cell migration Source: MGI
  5. chorio-allantoic fusion Source: MGI
  6. face development Source: MGI
  7. heart development Source: MGI
  8. heterophilic cell-cell adhesion Source: MGI
  9. integrin-mediated signaling pathway Source: UniProtKB-KW
  10. leukocyte cell-cell adhesion Source: MGI
  11. mesenchyme development Source: DFLAT
  12. negative regulation of protein homodimerization activity Source: UniProtKB
  13. single organismal cell-cell adhesion Source: DFLAT
  14. T cell migration Source: MGI
  15. visceral serous pericardium development Source: DFLAT
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-4
Alternative name(s):
CD49 antigen-like family member D
Integrin alpha-IV
Lymphocyte Peyer patch adhesion molecules subunit alpha
Short name:
LPAM subunit alpha
VLA-4 subunit alpha
CD_antigen: CD49d
Gene namesi
Name:Itga4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:96603. Itga4.

Subcellular locationi

GO - Cellular componenti

  1. cell-cell junction Source: MGI
  2. cell surface Source: DFLAT
  3. external side of plasma membrane Source: MGI
  4. integrin complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4040Sequence AnalysisAdd
BLAST
Chaini41 – 1039999Integrin alpha-4PRO_0000016245Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi86 – 861N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi98 ↔ 108By similarity
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi151 ↔ 172By similarity
Disulfide bondi190 ↔ 205By similarity
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi487 – 4871N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi493 ↔ 502By similarity
Disulfide bondi508 ↔ 564By similarity
Glycosylationi525 – 5251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi545 – 5451N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi629 ↔ 634By similarity
Glycosylationi633 – 6331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi652 – 6521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi667 – 6671N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi705 ↔ 718By similarity
Glycosylationi813 – 8131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi828 – 8281N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi859 ↔ 897By similarity
Disulfide bondi904 ↔ 909By similarity
Modified residuei1028 – 10281Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation on Ser-1028 inhibits PXN binding.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ00651.
PaxDbiQ00651.
PRIDEiQ00651.

PTM databases

PhosphoSiteiQ00651.

Expressioni

Tissue specificityi

Peyer patch homing cells.

Gene expression databases

CleanExiMM_ITGA4.
GenevestigatoriQ00651.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Interacts with AMICA1; integrin alpha-4/beta-1 may regulate leukocyte to endothelial cells adhesion by controlling AMICA1 homodimerization (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ00651.
SMRiQ00651. Positions 41-836.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini41 – 983943ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1008 – 103932CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei984 – 100724HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati42 – 10766FG-GAP 1Add
BLAST
Repeati117 – 18468FG-GAP 2Add
BLAST
Repeati193 – 24452FG-GAP 3Add
BLAST
Repeati245 – 30056FG-GAP 4Add
BLAST
Repeati301 – 35858FG-GAP 5Add
BLAST
Repeati362 – 41958FG-GAP 6Add
BLAST
Repeati423 – 48563FG-GAP 7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi613 – 62311SG1Add
BLAST
Motifi1010 – 10145GFFKR motif

Domaini

The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.By similarity

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated
Contains 7 FG-GAP repeats.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG324320.
HOGENOMiHOG000088626.
HOVERGENiHBG004538.
InParanoidiQ00651.
KOiK06483.
PhylomeDBiQ00651.

Family and domain databases

InterProiIPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view]
PfamiPF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 6 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00651-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFSTKSAWLR NGGADQGPRG IALREAVMLL LYFGVPTGPS YNLDPENALL
60 70 80 90 100
YQGPSGTLFG YSVVLHSHGS KRWLIVGAPT ASWLSNASVV NPGAIYRCGI
110 120 130 140 150
RKNPNQTCEQ LQSGSPSGEP CGKTCLEERD NQWLGVTLSR QPGENGSIVT
160 170 180 190 200
CGHRWKNIFY MKSDNKLPTG ICYVMPSDLR TELSKRMAPC YKDYTRKFGE
210 220 230 240 250
NFASCQAGIS SFYTQDLIVM GAPGSSYWTG TVFVYNITTN QYKAFVDRQN
260 270 280 290 300
QVKFGSYLGY SVGAGHFRSP HTTEVVGGAP QHEQIGKAYI FSIDENELNI
310 320 330 340 350
VYEMKGKKLG SYFGASVCAV DLNADGFSDL LVGAPMQSTI REEGRVFVYI
360 370 380 390 400
NSGMGAVMVE MERVLVGSDK YAARFGESIA NLGDIDNDGF EDIAIGAPQE
410 420 430 440 450
DDLRGAVYIY NGRVDGISST YSQRIEGQQI SKSLRMFGQS ISGQIDADNN
460 470 480 490 500
GYVDVAVGAF QSDSAVLLRT RPVVIVEASL SHPESVNRTK FDCTENGLPS
510 520 530 540 550
VCMHLTLCFS YKGKEVPGYI VLFYNVSLDV HRKAESPSRF YFFSNGTSDV
560 570 580 590 600
ITGSIRVSSS GEKCRTHQAF MRKDVRDILT PIHVEATYHL GHHVITKRNT
610 620 630 640 650
EEFPPLQPIL QQKKEKDVIR KMINFARFCA YENCSADLQV SAKVGFLKPY
660 670 680 690 700
ENKTYLAVGS MKTIMLNVSL FNAGDDAYET TLNVQLPTGL YFIKILDLEE
710 720 730 740 750
KQINCEVTES SGIVKLACSL GYIYVDRLSR IDISFLLDVS SLSRAHEDLS
760 770 780 790 800
ISVHASCENE GELDQVRDNR VTLTIPLRYE VMLTVHGLVN PTSFVYGSSE
810 820 830 840 850
ENEPETCMAE KLNLTFHVIN TGISMAPNVS VKIMVPNSFL PQDDKLFNVL
860 870 880 890 900
DVQTTTGQCH FKHYGRECTF AQQKGIAGTL TDIVKFLSKT DKRLLYCMKA
910 920 930 940 950
DQHCLDFLCN FGKMESGKEA SVHIQLEGRP SILEMDETSS LKFEIKATAF
960 970 980 990 1000
PEPHPKVIEL NKDENVAHVF LEGLHHQRPK RHFTIIIITI SLLLGLIVLL
1010 1020 1030
LISCVMWKAG FFKRQYKSIL QEENRRDSWS YVNSKSNDD
Length:1,039
Mass (Da):115,696
Last modified:April 1, 1993 - v1
Checksum:iC8C0BC611F7E9847
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53176 mRNA. Translation: CAA37316.1.
PIRiA41131.
RefSeqiNP_034706.3. NM_010576.3.
UniGeneiMm.31903.

Genome annotation databases

GeneIDi16401.
KEGGimmu:16401.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53176 mRNA. Translation: CAA37316.1 .
PIRi A41131.
RefSeqi NP_034706.3. NM_010576.3.
UniGenei Mm.31903.

3D structure databases

ProteinModelPortali Q00651.
SMRi Q00651. Positions 41-836.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL2111481.

PTM databases

PhosphoSitei Q00651.

Proteomic databases

MaxQBi Q00651.
PaxDbi Q00651.
PRIDEi Q00651.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 16401.
KEGGi mmu:16401.

Organism-specific databases

CTDi 3676.
MGIi MGI:96603. Itga4.

Phylogenomic databases

eggNOGi NOG324320.
HOGENOMi HOG000088626.
HOVERGENi HBG004538.
InParanoidi Q00651.
KOi K06483.
PhylomeDBi Q00651.

Miscellaneous databases

ChiTaRSi ITGA4. mouse.
NextBioi 289557.
PROi Q00651.
SOURCEi Search...

Gene expression databases

CleanExi MM_ITGA4.
Genevestigatori Q00651.

Family and domain databases

InterProi IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view ]
Pfami PF08441. Integrin_alpha2. 1 hit.
[Graphical view ]
PRINTSi PR01185. INTEGRINA.
SMARTi SM00191. Int_alpha. 6 hits.
[Graphical view ]
PROSITEi PS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of cDNAs for the alpha subunit of the murine lymphocyte-Peyer's patch adhesion molecule."
    Neuhaus H., Hu M.C.-T., Hemler M.E., Takada Y., Holzmann B., Weissman I.L.
    J. Cell Biol. 115:1149-1158(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1028, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiITA4_MOUSE
AccessioniPrimary (citable) accession number: Q00651
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3