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Q00651

- ITA4_MOUSE

UniProt

Q00651 - ITA4_MOUSE

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Protein
Integrin alpha-4
Gene
Itga4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Integrins alpha-4/beta-1 (VLA-4 or LPAM-2) and alpha-4/beta-7 (LPAM-1) are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. Mice homozygous for a null mutation of the alpha-4 subunit gene die by day E11-E14 from detachment and rupture of the epicardium and coronary arteries.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei598 – 5992Cleavage

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi321 – 3299 Reviewed prediction
Calcium bindingi384 – 3929 Reviewed prediction
Calcium bindingi446 – 4549 Reviewed prediction

GO - Molecular functioni

  1. fibronectin binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. T cell migration Source: MGI
  2. blood vessel remodeling Source: MGI
  3. cell adhesion Source: MGI
  4. cell migration Source: MGI
  5. cell-matrix adhesion Source: DFLAT
  6. chorio-allantoic fusion Source: MGI
  7. face development Source: MGI
  8. heart development Source: MGI
  9. heterophilic cell-cell adhesion Source: MGI
  10. integrin-mediated signaling pathway Source: UniProtKB-KW
  11. leukocyte cell-cell adhesion Source: MGI
  12. mesenchyme development Source: DFLAT
  13. negative regulation of protein homodimerization activity Source: UniProtKB
  14. single organismal cell-cell adhesion Source: DFLAT
  15. visceral serous pericardium development Source: DFLAT
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-4
Alternative name(s):
CD49 antigen-like family member D
Integrin alpha-IV
Lymphocyte Peyer patch adhesion molecules subunit alpha
Short name:
LPAM subunit alpha
VLA-4 subunit alpha
CD_antigen: CD49d
Gene namesi
Name:Itga4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:96603. Itga4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini41 – 983943Extracellular Reviewed prediction
Add
BLAST
Transmembranei984 – 100724Helical; Reviewed prediction
Add
BLAST
Topological domaini1008 – 103932Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cell surface Source: DFLAT
  2. cell-cell junction Source: MGI
  3. external side of plasma membrane Source: MGI
  4. integrin complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4040 Reviewed prediction
Add
BLAST
Chaini41 – 1039999Integrin alpha-4
PRO_0000016245Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi86 – 861N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi98 ↔ 108 By similarity
Glycosylationi105 – 1051N-linked (GlcNAc...) Reviewed prediction
Glycosylationi145 – 1451N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi151 ↔ 172 By similarity
Disulfide bondi190 ↔ 205 By similarity
Glycosylationi236 – 2361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi487 – 4871N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi493 ↔ 502 By similarity
Disulfide bondi508 ↔ 564 By similarity
Glycosylationi525 – 5251N-linked (GlcNAc...) Reviewed prediction
Glycosylationi545 – 5451N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi629 ↔ 634 By similarity
Glycosylationi633 – 6331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi652 – 6521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi667 – 6671N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi705 ↔ 718 By similarity
Glycosylationi813 – 8131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi828 – 8281N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi859 ↔ 897 By similarity
Disulfide bondi904 ↔ 909 By similarity
Modified residuei1028 – 10281Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation on Ser-1028 inhibits PXN binding By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ00651.
PaxDbiQ00651.
PRIDEiQ00651.

PTM databases

PhosphoSiteiQ00651.

Expressioni

Tissue specificityi

Peyer patch homing cells.

Gene expression databases

CleanExiMM_ITGA4.
GenevestigatoriQ00651.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Interacts with AMICA1; integrin alpha-4/beta-1 may regulate leukocyte to endothelial cells adhesion by controlling AMICA1 homodimerization By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ00651.
SMRiQ00651. Positions 41-836, 983-1022.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati42 – 10766FG-GAP 1
Add
BLAST
Repeati117 – 18468FG-GAP 2
Add
BLAST
Repeati193 – 24452FG-GAP 3
Add
BLAST
Repeati245 – 30056FG-GAP 4
Add
BLAST
Repeati301 – 35858FG-GAP 5
Add
BLAST
Repeati362 – 41958FG-GAP 6
Add
BLAST
Repeati423 – 48563FG-GAP 7
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi613 – 62311SG1
Add
BLAST
Motifi1010 – 10145GFFKR motif

Domaini

The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion By similarity.

Sequence similaritiesi

Contains 7 FG-GAP repeats.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG324320.
HOGENOMiHOG000088626.
HOVERGENiHBG004538.
InParanoidiQ00651.
KOiK06483.
PhylomeDBiQ00651.

Family and domain databases

InterProiIPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view]
PfamiPF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 6 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00651-1 [UniParc]FASTAAdd to Basket

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MFSTKSAWLR NGGADQGPRG IALREAVMLL LYFGVPTGPS YNLDPENALL     50
YQGPSGTLFG YSVVLHSHGS KRWLIVGAPT ASWLSNASVV NPGAIYRCGI 100
RKNPNQTCEQ LQSGSPSGEP CGKTCLEERD NQWLGVTLSR QPGENGSIVT 150
CGHRWKNIFY MKSDNKLPTG ICYVMPSDLR TELSKRMAPC YKDYTRKFGE 200
NFASCQAGIS SFYTQDLIVM GAPGSSYWTG TVFVYNITTN QYKAFVDRQN 250
QVKFGSYLGY SVGAGHFRSP HTTEVVGGAP QHEQIGKAYI FSIDENELNI 300
VYEMKGKKLG SYFGASVCAV DLNADGFSDL LVGAPMQSTI REEGRVFVYI 350
NSGMGAVMVE MERVLVGSDK YAARFGESIA NLGDIDNDGF EDIAIGAPQE 400
DDLRGAVYIY NGRVDGISST YSQRIEGQQI SKSLRMFGQS ISGQIDADNN 450
GYVDVAVGAF QSDSAVLLRT RPVVIVEASL SHPESVNRTK FDCTENGLPS 500
VCMHLTLCFS YKGKEVPGYI VLFYNVSLDV HRKAESPSRF YFFSNGTSDV 550
ITGSIRVSSS GEKCRTHQAF MRKDVRDILT PIHVEATYHL GHHVITKRNT 600
EEFPPLQPIL QQKKEKDVIR KMINFARFCA YENCSADLQV SAKVGFLKPY 650
ENKTYLAVGS MKTIMLNVSL FNAGDDAYET TLNVQLPTGL YFIKILDLEE 700
KQINCEVTES SGIVKLACSL GYIYVDRLSR IDISFLLDVS SLSRAHEDLS 750
ISVHASCENE GELDQVRDNR VTLTIPLRYE VMLTVHGLVN PTSFVYGSSE 800
ENEPETCMAE KLNLTFHVIN TGISMAPNVS VKIMVPNSFL PQDDKLFNVL 850
DVQTTTGQCH FKHYGRECTF AQQKGIAGTL TDIVKFLSKT DKRLLYCMKA 900
DQHCLDFLCN FGKMESGKEA SVHIQLEGRP SILEMDETSS LKFEIKATAF 950
PEPHPKVIEL NKDENVAHVF LEGLHHQRPK RHFTIIIITI SLLLGLIVLL 1000
LISCVMWKAG FFKRQYKSIL QEENRRDSWS YVNSKSNDD 1039
Length:1,039
Mass (Da):115,696
Last modified:April 1, 1993 - v1
Checksum:iC8C0BC611F7E9847
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53176 mRNA. Translation: CAA37316.1.
PIRiA41131.
RefSeqiNP_034706.3. NM_010576.3.
UniGeneiMm.31903.

Genome annotation databases

GeneIDi16401.
KEGGimmu:16401.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53176 mRNA. Translation: CAA37316.1 .
PIRi A41131.
RefSeqi NP_034706.3. NM_010576.3.
UniGenei Mm.31903.

3D structure databases

ProteinModelPortali Q00651.
SMRi Q00651. Positions 41-836, 983-1022.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL2111481.

PTM databases

PhosphoSitei Q00651.

Proteomic databases

MaxQBi Q00651.
PaxDbi Q00651.
PRIDEi Q00651.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 16401.
KEGGi mmu:16401.

Organism-specific databases

CTDi 3676.
MGIi MGI:96603. Itga4.

Phylogenomic databases

eggNOGi NOG324320.
HOGENOMi HOG000088626.
HOVERGENi HBG004538.
InParanoidi Q00651.
KOi K06483.
PhylomeDBi Q00651.

Miscellaneous databases

ChiTaRSi ITGA4. mouse.
NextBioi 289557.
PROi Q00651.
SOURCEi Search...

Gene expression databases

CleanExi MM_ITGA4.
Genevestigatori Q00651.

Family and domain databases

InterProi IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view ]
Pfami PF08441. Integrin_alpha2. 1 hit.
[Graphical view ]
PRINTSi PR01185. INTEGRINA.
SMARTi SM00191. Int_alpha. 6 hits.
[Graphical view ]
PROSITEi PS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of cDNAs for the alpha subunit of the murine lymphocyte-Peyer's patch adhesion molecule."
    Neuhaus H., Hu M.C.-T., Hemler M.E., Takada Y., Holzmann B., Weissman I.L.
    J. Cell Biol. 115:1149-1158(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1028, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiITA4_MOUSE
AccessioniPrimary (citable) accession number: Q00651
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 9, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi