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Protein

Myosin-1

Gene

myoA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Type-I myosin implicated in the organization of the actin cytoskeleton. Required for proper actin cytoskeleton polarization. At the cell cortex, assembles in patch-like structures together with proteins from the actin-polymerizing machinery and promotes actin assembly. Functions as actin nucleation-promoting factor (NPF) for the Arp2/3 complex (By similarity). Plays an important role in polarized growth, spore germination, hyphal morphogenesis, and septal wall formation.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi143 – 150ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • actin cortical patch localization Source: ASPGD
  • cell tip growth Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-1
Alternative name(s):
Class I unconventional myosin
Type I myosin
Gene namesi
Name:myoA
ORF Names:AN1558
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VII
  • UP000005890 Componenti: Partially assembled WGS sequence

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: ASPGD
  • cell septum Source: ASPGD
  • hyphal tip Source: ASPGD
  • myosin complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003385511 – 1249Myosin-1Add BLAST1249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei371PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation of the TEDS site (Ser-371) is required for the polarization of the actin cytoskeleton. Phosphorylation probably activates the myosin-I ATPase activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Found in dormant conidiospores.1 Publication

Interactioni

Subunit structurei

Interacts (via IQ domains) with camA.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ00647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 729Myosin motorAdd BLAST680
Domaini733 – 753IQ 1Add BLAST21
Domaini754 – 779IQ 2Add BLAST26
Domaini787 – 979TH1PROSITE-ProRule annotationAdd BLAST193
Domaini1074 – 1135SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni418 – 500Actin-bindingBy similarityAdd BLAST83

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi970 – 1194Pro-richAdd BLAST225
Compositional biasi1029 – 1081Ala-richAdd BLAST53
Compositional biasi1149 – 1193Ala-richAdd BLAST45
Compositional biasi1244 – 1248Poly-Asp5

Domaini

The myosin motor domain displays actin-stimulated ATPase activity and generates a mechanochemical force.By similarity
The tail domain participates in molecular interactions that specify the role of the motor domain (By similarity). It is composed of several tail homology (TH) domains, namely a putative phospholipid-binding myosin tail domain (also named TH1), an Ala- and Pro-rich domain (TH2), followed by an SH3 domain and a C-terminal acidic domain (TH3).By similarity

Sequence similaritiesi

Contains 2 IQ domains.Curated
Contains 1 myosin motor domain.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 TH1 (class I myosin tail homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

HOGENOMiHOG000260265.
InParanoidiQ00647.
KOiK10356.
OMAiLKLRFEH.
OrthoDBiEOG092C0QJU.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51456. MYOSIN_MOTOR. 1 hit.
PS50002. SH3. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHSRRPAGG EKKSRFGRSK AAADVGDGRQ AGGKPQVRKA VFESTKKKEI
60 70 80 90 100
GVSDLTLLSK ISNEAINDNL KLRFQHDEIY TYIGHVLVSV NPFRDLGIYT
110 120 130 140 150
DSVLNSYRGK NRLEVPPHVF AVAESAYYNM KSYKDNQCVI ISGESGAGKT
160 170 180 190 200
EAAKRIMQYI ASVSGGSDSS IQQTKDMVLA TNPLLESFGN AKTLRNNNSS
210 220 230 240 250
RFGKYLELEF NAQGEPVGAN ITNYLLEKSR VVGQITNERN FHIFYQFAKG
260 270 280 290 300
APQKYRDSFG VQQPQSYLYT SRSKCFDVPG VDDVAEFQDT LNAMSVIGMS
310 320 330 340 350
EAEQDNVFRM LAAILWMGNI QFAEDDSGNA AITDQSVVDF VAYLLEVDAG
360 370 380 390 400
QVNQALTIRM METSRGGRRG SVYEVPLNTT QALAVRDALA KAIYFNLFDW
410 420 430 440 450
IVGRVNQSLT AKGAVANSIG ILDIYGFEIF EKNSFEQLCI NYVNEKLQQI
460 470 480 490 500
FIQLTLKAEQ DEYEREQITW TPIKYFDNKV VCSLIEDKRP PGVFAALNDA
510 520 530 540 550
CATAHADSGA ADNTFVGRLN FLGQNPNFEN RQGQFIIKHY AGDVSYAVQG
560 570 580 590 600
MTDKNKDQLL KDLLNLVQSS SNHFVHTLFP EQVNQDDKRR PPTASDKIKA
610 620 630 640 650
SANDLVATLM KAQPSYIRTI KPNDNKAPKE FNESNVLHQI KYLGLQENVR
660 670 680 690 700
IRRAGFAYRQ TFDKFVERFY LLSPKTSYAG DYTWTGDVET GARQILKDTR
710 720 730 740 750
IPAEEYQMGI TKVFIKTPET LFALEAMRDR YWHNMAIRIQ RAWRNYLRYR
760 770 780 790 800
TECAIRIQRF WRRMNGGLEL LKLRDQGHTI LGGRKERRRM SILGSRRFLG
810 820 830 840 850
DYVGISNKGG PGEMIRSGAA ISTSDDVLFS CRGEVLVSKF GRSSKPSPRI
860 870 880 890 900
FVLTNRHVYI VSQNFVNNQL VISSERTIPI GAIKTVSASS YRDDWFSLVV
910 920 930 940 950
GGQEPDPLCN CVFKTEFFTH LHNALRGQLN LKIGPEIEYN KKPGKLATVK
960 970 980 990 1000
VVKDGSQVDS YKSGTIHTGP GEPPNSVSKP TPRGKQVAAR PVTKGKLLRP
1010 1020 1030 1040 1050
GGPGGGPSKL ASRPVPERRP IPQPTPQTAA AQPTPASRPV PQPVAAVAAS
1060 1070 1080 1090 1100
HSRTSSTASA RAPPPPPPAP PAAAGPKKAK ALYDFSSDNN GMLSISAGQI
1110 1120 1130 1140 1150
VEIVSKEGNG WWLCMNLETS AQGWTPEAYL EEQVAPTPKP APPPPPPVAP
1160 1170 1180 1190 1200
RASPAPVNGS AAVAAAKAKA APPPPAKRPN MAGRKTAPAP PPAPRDSAVS
1210 1220 1230 1240
MNSQGDSSGA SGRGTPSSVS NASLAGGLAE ALRARQSAMQ GKQDDDDDW
Length:1,249
Mass (Da):137,074
Last modified:June 10, 2008 - v2
Checksum:i6F750DBA67B25186
GO

Sequence cautioni

The sequence AAA67877 differs from that shown. Reason: Frameshift at positions 1000 and 1069.Curated
The sequence EAA64265 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti608T → M in AAA67877 (PubMed:7860631).Curated1
Sequence conflicti762R → P in AAA67877 (PubMed:7860631).Curated1
Sequence conflicti1223S → C in AAA67877 (PubMed:7860631).Curated1
Sequence conflicti1234A → R in AAA67877 (PubMed:7860631).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12427 mRNA. Translation: AAA67877.1. Frameshift.
AACD01000025 Genomic DNA. Translation: EAA64265.1. Sequence problems.
BN001307 Genomic DNA. Translation: CBF85107.1.
PIRiA56511.
RefSeqiXP_659162.1. XM_654070.1.

Genome annotation databases

EnsemblFungiiCADANIAT00008187; CADANIAP00008187; CADANIAG00008187.
EAA64265; EAA64265; AN1558.2.
GeneIDi2875672.
KEGGiani:AN1558.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12427 mRNA. Translation: AAA67877.1. Frameshift.
AACD01000025 Genomic DNA. Translation: EAA64265.1. Sequence problems.
BN001307 Genomic DNA. Translation: CBF85107.1.
PIRiA56511.
RefSeqiXP_659162.1. XM_654070.1.

3D structure databases

ProteinModelPortaliQ00647.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00008187; CADANIAP00008187; CADANIAG00008187.
EAA64265; EAA64265; AN1558.2.
GeneIDi2875672.
KEGGiani:AN1558.2.

Phylogenomic databases

HOGENOMiHOG000260265.
InParanoidiQ00647.
KOiK10356.
OMAiLKLRFEH.
OrthoDBiEOG092C0QJU.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00242. MYSc. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51456. MYOSIN_MOTOR. 1 hit.
PS50002. SH3. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYO1_EMENI
AccessioniPrimary (citable) accession number: Q00647
Secondary accession number(s): C8VMZ7, Q5BD22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: November 30, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.