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Protein

Myosin-1

Gene

myoA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Type-I myosin implicated in the organization of the actin cytoskeleton. Required for proper actin cytoskeleton polarization. At the cell cortex, assembles in patch-like structures together with proteins from the actin-polymerizing machinery and promotes actin assembly. Functions as actin nucleation-promoting factor (NPF) for the Arp2/3 complex (By similarity). Plays an important role in polarized growth, spore germination, hyphal morphogenesis, and septal wall formation.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi143 – 150ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • actin cortical patch localization Source: AspGD
  • cell tip growth Source: AspGD
  • microtubule-based movement Source: InterPro

Keywordsi

Molecular functionActin-binding, Hydrolase, Motor protein, Myosin
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-1
Alternative name(s):
Class I unconventional myosin
Type I myosin
Gene namesi
Name:myoA
ORF Names:AN1558
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VII
  • UP000005890 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003385511 – 1249Myosin-1Add BLAST1249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei371PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation of the TEDS site (Ser-371) is required for the polarization of the actin cytoskeleton. Phosphorylation probably activates the myosin-I ATPase activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ00647

Expressioni

Developmental stagei

Found in dormant conidiospores.1 Publication

Interactioni

Subunit structurei

Interacts (via IQ domains) with camA.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00008187

Structurei

3D structure databases

ProteinModelPortaliQ00647
SMRiQ00647
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 729Myosin motorPROSITE-ProRule annotationAdd BLAST680
Domaini733 – 753IQ 1Add BLAST21
Domaini754 – 779IQ 2Add BLAST26
Domaini787 – 979TH1PROSITE-ProRule annotationAdd BLAST193
Domaini1074 – 1135SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni418 – 500Actin-bindingBy similarityAdd BLAST83

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi970 – 1194Pro-richAdd BLAST225
Compositional biasi1029 – 1081Ala-richAdd BLAST53
Compositional biasi1149 – 1193Ala-richAdd BLAST45
Compositional biasi1244 – 1248Poly-Asp5

Domaini

The myosin motor domain displays actin-stimulated ATPase activity and generates a mechanochemical force.By similarity
The tail domain participates in molecular interactions that specify the role of the motor domain (By similarity). It is composed of several tail homology (TH) domains, namely a putative phospholipid-binding myosin tail domain (also named TH1), an Ala- and Pro-rich domain (TH2), followed by an SH3 domain and a C-terminal acidic domain (TH3).By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

HOGENOMiHOG000260265
InParanoidiQ00647
KOiK10356
OMAiKENWGPW
OrthoDBiEOG092C0QJU

Family and domain databases

CDDicd01378 MYSc_Myo1, 1 hit
Gene3Di3.40.850.10, 3 hits
InterProiView protein in InterPro
IPR036961 Kinesin_motor_dom_sf
IPR001609 Myosin_head_motor_dom
IPR010926 Myosin_TH1
IPR036072 MYSc_Myo1
IPR027417 P-loop_NTPase
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00063 Myosin_head, 1 hit
PF06017 Myosin_TH1, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM00242 MYSc, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51456 MYOSIN_MOTOR, 1 hit
PS50002 SH3, 1 hit
PS51757 TH1, 1 hit

Sequencei

Sequence statusi: Complete.

Q00647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHSRRPAGG EKKSRFGRSK AAADVGDGRQ AGGKPQVRKA VFESTKKKEI
60 70 80 90 100
GVSDLTLLSK ISNEAINDNL KLRFQHDEIY TYIGHVLVSV NPFRDLGIYT
110 120 130 140 150
DSVLNSYRGK NRLEVPPHVF AVAESAYYNM KSYKDNQCVI ISGESGAGKT
160 170 180 190 200
EAAKRIMQYI ASVSGGSDSS IQQTKDMVLA TNPLLESFGN AKTLRNNNSS
210 220 230 240 250
RFGKYLELEF NAQGEPVGAN ITNYLLEKSR VVGQITNERN FHIFYQFAKG
260 270 280 290 300
APQKYRDSFG VQQPQSYLYT SRSKCFDVPG VDDVAEFQDT LNAMSVIGMS
310 320 330 340 350
EAEQDNVFRM LAAILWMGNI QFAEDDSGNA AITDQSVVDF VAYLLEVDAG
360 370 380 390 400
QVNQALTIRM METSRGGRRG SVYEVPLNTT QALAVRDALA KAIYFNLFDW
410 420 430 440 450
IVGRVNQSLT AKGAVANSIG ILDIYGFEIF EKNSFEQLCI NYVNEKLQQI
460 470 480 490 500
FIQLTLKAEQ DEYEREQITW TPIKYFDNKV VCSLIEDKRP PGVFAALNDA
510 520 530 540 550
CATAHADSGA ADNTFVGRLN FLGQNPNFEN RQGQFIIKHY AGDVSYAVQG
560 570 580 590 600
MTDKNKDQLL KDLLNLVQSS SNHFVHTLFP EQVNQDDKRR PPTASDKIKA
610 620 630 640 650
SANDLVATLM KAQPSYIRTI KPNDNKAPKE FNESNVLHQI KYLGLQENVR
660 670 680 690 700
IRRAGFAYRQ TFDKFVERFY LLSPKTSYAG DYTWTGDVET GARQILKDTR
710 720 730 740 750
IPAEEYQMGI TKVFIKTPET LFALEAMRDR YWHNMAIRIQ RAWRNYLRYR
760 770 780 790 800
TECAIRIQRF WRRMNGGLEL LKLRDQGHTI LGGRKERRRM SILGSRRFLG
810 820 830 840 850
DYVGISNKGG PGEMIRSGAA ISTSDDVLFS CRGEVLVSKF GRSSKPSPRI
860 870 880 890 900
FVLTNRHVYI VSQNFVNNQL VISSERTIPI GAIKTVSASS YRDDWFSLVV
910 920 930 940 950
GGQEPDPLCN CVFKTEFFTH LHNALRGQLN LKIGPEIEYN KKPGKLATVK
960 970 980 990 1000
VVKDGSQVDS YKSGTIHTGP GEPPNSVSKP TPRGKQVAAR PVTKGKLLRP
1010 1020 1030 1040 1050
GGPGGGPSKL ASRPVPERRP IPQPTPQTAA AQPTPASRPV PQPVAAVAAS
1060 1070 1080 1090 1100
HSRTSSTASA RAPPPPPPAP PAAAGPKKAK ALYDFSSDNN GMLSISAGQI
1110 1120 1130 1140 1150
VEIVSKEGNG WWLCMNLETS AQGWTPEAYL EEQVAPTPKP APPPPPPVAP
1160 1170 1180 1190 1200
RASPAPVNGS AAVAAAKAKA APPPPAKRPN MAGRKTAPAP PPAPRDSAVS
1210 1220 1230 1240
MNSQGDSSGA SGRGTPSSVS NASLAGGLAE ALRARQSAMQ GKQDDDDDW
Length:1,249
Mass (Da):137,074
Last modified:June 10, 2008 - v2
Checksum:i6F750DBA67B25186
GO

Sequence cautioni

The sequence AAA67877 differs from that shown. Reason: Frameshift at positions 1000 and 1069.Curated
The sequence EAA64265 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti608T → M in AAA67877 (PubMed:7860631).Curated1
Sequence conflicti762R → P in AAA67877 (PubMed:7860631).Curated1
Sequence conflicti1223S → C in AAA67877 (PubMed:7860631).Curated1
Sequence conflicti1234A → R in AAA67877 (PubMed:7860631).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12427 mRNA Translation: AAA67877.1 Frameshift.
AACD01000025 Genomic DNA Translation: EAA64265.1 Sequence problems.
BN001307 Genomic DNA Translation: CBF85107.1
PIRiA56511
RefSeqiXP_659162.1, XM_654070.1

Genome annotation databases

EnsemblFungiiCADANIAT00008187; CADANIAP00008187; CADANIAG00008187
EAA64265; EAA64265; AN1558.2
GeneIDi2875672
KEGGiani:AN1558.2

Similar proteinsi

Entry informationi

Entry nameiMYO1_EMENI
AccessioniPrimary (citable) accession number: Q00647
Secondary accession number(s): C8VMZ7, Q5BD22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: May 23, 2018
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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