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Q00646

- CDK1_EMENI

UniProt

Q00646 - CDK1_EMENI

Protein

Cyclin-dependent kinase 1

Gene

nimX

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-180 activates it.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341ATPPROSITE-ProRule annotation
    Active sitei147 – 1471Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: ASPGD
    3. protein kinase activity Source: ASPGD
    4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular response to light stimulus Source: ASPGD
    2. conidium formation Source: ASPGD
    3. Hulle cell development Source: ASPGD
    4. mitotic nuclear division Source: UniProtKB-KW
    5. negative regulation of Hulle cell development Source: ASPGD
    6. negative regulation of sexual sporulation resulting in formation of a cellular spore Source: ASPGD
    7. positive regulation of mitotic cell cycle Source: ASPGD
    8. positive regulation of sterigmatocystin biosynthetic process Source: ASPGD
    9. protein phosphorylation Source: ASPGD
    10. regulation of conidium formation Source: ASPGD
    11. sexual sporulation resulting in formation of a cellular spore Source: ASPGD
    12. sporocarp development involved in sexual reproduction Source: ASPGD
    13. sterigmatocystin biosynthetic process Source: ASPGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 2065.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDK1
    Alternative name(s):
    Cell division control protein 2
    Cell division protein kinase 1
    Never in mitosis protein X
    Gene namesi
    Name:nimX
    Synonyms:cdk1
    ORF Names:AN4182
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome II

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: ASPGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 323323Cyclin-dependent kinase 1PRO_0000085719Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei14 – 141PhosphothreonineBy similarity
    Modified residuei15 – 151PhosphotyrosineBy similarity
    Modified residuei180 – 1801Phosphothreonine; by CAKBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ00646.

    Interactioni

    Subunit structurei

    Forms a stable but non-covalent complex with a regulatory subunit (SUC1) and with a cyclin.

    Protein-protein interaction databases

    MINTiMINT-242233.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00646.
    SMRiQ00646. Positions 1-307.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 306303Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    KOiK04563.
    OMAiLVHRFFR.
    OrthoDBiEOG7K3TWD.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00646-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENYQKIEKI GEGTYGVVYK ARELTHPNRI VALKKIRLEA EDEGVPSTAI    50
    REISLLKEMN DPNIVRLLNI VHADGHKLYL VFEFLDLDLK KYMEALPVSE 100
    GGRGRALPDG STLSRNLGLG DAMVKKFMAQ LIEGIRFCHS HRVLHRDLKP 150
    QNLLIDRDGN LKLADFGLAR AFGVPLRTYT HEVVTLWYRS PEILLGGRQY 200
    STGVDMWSCG AIFAEMCTRK PLFPGDSEID EIFKIFRILG TPDETIWPGV 250
    TSFPDFKPTF PKWKREDIQN VVPGLEEDGL DLLEALLEYD PARRISAKQA 300
    CMHPYFQHGS SYYSGRARRN GFH 323
    Length:323
    Mass (Da):36,779
    Last modified:November 1, 1996 - v1
    Checksum:i3178CAFBDDC32224
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07169 mRNA. Translation: AAA20597.1.
    AACD01000068 Genomic DNA. Translation: EAA59281.1.
    BN001302 Genomic DNA. Translation: CBF74540.1.
    RefSeqiXP_661786.1. XM_656694.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00004488; CADANIAP00004488; CADANIAG00004488.
    GeneIDi2873604.
    KEGGiani:AN4182.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07169 mRNA. Translation: AAA20597.1 .
    AACD01000068 Genomic DNA. Translation: EAA59281.1 .
    BN001302 Genomic DNA. Translation: CBF74540.1 .
    RefSeqi XP_661786.1. XM_656694.1.

    3D structure databases

    ProteinModelPortali Q00646.
    SMRi Q00646. Positions 1-307.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-242233.

    Proteomic databases

    PRIDEi Q00646.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00004488 ; CADANIAP00004488 ; CADANIAG00004488 .
    GeneIDi 2873604.
    KEGGi ani:AN4182.2.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    KOi K04563.
    OMAi LVHRFFR.
    OrthoDBi EOG7K3TWD.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 2065.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A single p34cdc2 protein kinase (encoded by nimXcdc2) is required at G1 and G2 in Aspergillus nidulans."
      Osmani A.H., van Peij N., Mischke M., O'Connell M.J., Osmani S.A.
      J. Cell Sci. 107:1519-1528(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Mycelium.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    3. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

    Entry informationi

    Entry nameiCDK1_EMENI
    AccessioniPrimary (citable) accession number: Q00646
    Secondary accession number(s): C8V4M9, Q5B5J8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3