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Protein

Apolipoprotein A-I

Gene

Apoa1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCholesterol metabolism, Lipid metabolism, Lipid transport, Steroid metabolism, Sterol metabolism, Transport

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-1369062 ABC transporters in lipid homeostasis
R-MMU-2168880 Scavenging of heme from plasma
R-MMU-3000471 Scavenging by Class B Receptors
R-MMU-3000480 Scavenging by Class A Receptors
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-8957275 Post-translational protein phosphorylation
R-MMU-8963888 Chylomicron assembly
R-MMU-8963896 HDL assembly
R-MMU-8963901 Chylomicron remodeling
R-MMU-8964011 HDL clearance
R-MMU-8964058 HDL remodeling
R-MMU-975634 Retinoid metabolism and transport

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein A-I
Short name:
Apo-AI
Short name:
ApoA-I
Alternative name(s):
Apolipoprotein A1
Cleaved into the following 2 chains:
Gene namesi
Name:Apoa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88049 Apoa1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000042532619 – 264Proapolipoprotein A-IAdd BLAST246
ChainiPRO_000000194625 – 264Apolipoprotein A-IAdd BLAST240
ChainiPRO_000041657625 – 263Truncated apolipoprotein A-IBy similarityAdd BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei109Methionine sulfoxideBy similarity1
Modified residuei193Methionine sulfoxide1 Publication1
Modified residuei240Methionine sulfoxide1 Publication1
Modified residuei242Methionine sulfoxide1 Publication1

Post-translational modificationi

Glycosylated.By similarity
Palmitoylated.By similarity
May be acylated.
Phosphorylation sites are present in the extracellular medium.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Oxidation, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ00623
PeptideAtlasiQ00623
PRIDEiQ00623

2D gel databases

REPRODUCTION-2DPAGEiIPI00121209
Q00623
SWISS-2DPAGEiQ00623

PTM databases

iPTMnetiQ00623
PhosphoSitePlusiQ00623

Expressioni

Tissue specificityi

Major protein of plasma HDL, also found in chylomicrons.

Gene expression databases

BgeeiENSMUSG00000032083
CleanExiMM_APOA1
ExpressionAtlasiQ00623 baseline and differential
GenevisibleiQ00623 MM

Interactioni

Subunit structurei

Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Interacts with SCGB3A2.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Kcnma1Q084604EBI-1634106,EBI-1633915

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198155, 6 interactors
IntActiQ00623, 5 interactors
MINTiQ00623
STRINGi10090.ENSMUSP00000034588

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 71Combined sources42
Turni72 – 75Combined sources4
Helixi83 – 119Combined sources37
Turni120 – 123Combined sources4
Helixi126 – 141Combined sources16
Helixi145 – 154Combined sources10
Helixi167 – 206Combined sources40
Beta strandi217 – 219Combined sources3
Helixi220 – 226Combined sources7
Helixi233 – 237Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LEMNMR-A25-240[»]
ProteinModelPortaliQ00623
SMRiQ00623
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati67 – 881Add BLAST22
Repeati89 – 1102Add BLAST22
Repeati111 – 1213; half-lengthAdd BLAST11
Repeati122 – 1434Add BLAST22
Repeati144 – 1655Add BLAST22
Repeati166 – 1876Add BLAST22
Repeati188 – 2077; truncatedAdd BLAST20
Repeati208 – 2298Add BLAST22
Repeati230 – 2409; half-lengthAdd BLAST11
Repeati241 – 26410Add BLAST24

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 26410 X approximate tandem repeatsAdd BLAST198

Sequence similaritiesi

Belongs to the apolipoprotein A1/A4/E family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IWKR Eukaryota
ENOG410YGQ6 LUCA
GeneTreeiENSGT00530000063081
HOGENOMiHOG000033998
HOVERGENiHBG105708
InParanoidiQ00623
KOiK08757
OMAiKDFATVY
OrthoDBiEOG091G0IA5
TreeFamiTF334458

Family and domain databases

InterProiView protein in InterPro
IPR000074 ApoA_E
PfamiView protein in Pfam
PF01442 Apolipoprotein, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVVLAVAL VFLTGSQAWH VWQQDEPQSQ WDKVKDFANV YVDAVKDSGR
60 70 80 90 100
DYVSQFESSS LGQQLNLNLL ENWDTLGSTV SQLQERLGPL TRDFWDNLEK
110 120 130 140 150
ETDWVRQEMN KDLEEVKQKV QPYLDEFQKK WKEDVELYRQ KVAPLGAELQ
160 170 180 190 200
ESARQKLQEL QGRLSPVAEE FRDRMRTHVD SLRTQLAPHS EQMRESLAQR
210 220 230 240 250
LAELKSNPTL NEYHTRAKTH LKTLGEKARP ALEDLRHSLM PMLETLKTQV
260
QSVIDKASET LTAQ
Length:264
Mass (Da):30,616
Last modified:October 3, 2012 - v2
Checksum:i5E33FF201963583B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143A → G in AAB58424 (Ref. 3) Curated1
Sequence conflicti143A → G in AAB58425 (Ref. 3) Curated1
Sequence conflicti143A → G in AAB58426 (Ref. 3) Curated1
Sequence conflicti143A → G in AAB58427 (Ref. 3) Curated1
Sequence conflicti146Missing in AAB58424 (Ref. 3) Curated1
Sequence conflicti146Missing in AAB58425 (Ref. 3) Curated1
Sequence conflicti146Missing in AAB58426 (Ref. 3) Curated1
Sequence conflicti146Missing in AAB58427 (Ref. 3) Curated1

Mass spectrometryi

Molecular mass is 27951.3±1.343 Da from positions 25 - 264. Determined by ESI. Strain C57BL/6. Without methionine sulfoxide.1 Publication
Molecular mass is 27923.8 Da from positions 25 - 264. Determined by ESI. Strain BALB/c. Without methionine sulfoxide.1 Publication
Molecular mass is 27965 Da from positions 25 - 264. Determined by ESI. Strain C57BL/6. With 1 methionine sulfoxide.1 Publication
Molecular mass is 28819.7 Da from positions 19 - 264. Determined by ESI. Strain C57BL/6. Without methionine sulfoxide.1 Publication
Molecular mass is 28790.7 Da from positions 19 - 264. Determined by ESI. Strain BALB/c. Without methionine sulfoxide.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti249 – 250QV → KA in strain: BALB/c, C3H and ICR. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64262 mRNA Translation: CAA45560.1
X64263 Genomic DNA Translation: CAA45561.1
L04149 Genomic DNA No translation available.
L04151 mRNA No translation available.
U79572 Genomic DNA Translation: AAB58424.1
U79574 Genomic DNA Translation: AAB58426.1
U79573 Genomic DNA Translation: AAB58425.1
U79575 Genomic DNA Translation: AAB58427.1
AK076187 mRNA Translation: BAC36241.1
AK149576 mRNA Translation: BAE28968.1
AK161536 mRNA Translation: BAE36448.1
AC116503 Genomic DNA No translation available.
BC012253 mRNA Translation: AAH12253.1
BC019837 mRNA Translation: AAH19837.1
CCDSiCCDS40610.1
PIRiS22420
RefSeqiNP_033822.2, NM_009692.4
UniGeneiMm.26743

Genome annotation databases

EnsembliENSMUST00000034588; ENSMUSP00000034588; ENSMUSG00000032083
GeneIDi11806
KEGGimmu:11806
UCSCiuc009phb.3 mouse

Similar proteinsi

Entry informationi

Entry nameiAPOA1_MOUSE
AccessioniPrimary (citable) accession number: Q00623
Secondary accession number(s): O08855, O09042, Q8BPD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 3, 2012
Last modified: June 20, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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