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Q00623

- APOA1_MOUSE

UniProt

Q00623 - APOA1_MOUSE

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Protein
Apolipoprotein A-I
Gene
Apoa1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

GO - Molecular functioni

  1. beta-amyloid binding Source: Ensembl
  2. cholesterol binding Source: Ensembl
  3. cholesterol transporter activity Source: MGI
  4. high-density lipoprotein particle binding Source: MGI
  5. identical protein binding Source: MGI
  6. lipase inhibitor activity Source: Ensembl
  7. lipid binding Source: MGI
  8. lipid transporter activity Source: MGI
  9. lipoprotein particle binding Source: MGI
  10. phosphatidylcholine-sterol O-acyltransferase activator activity Source: Ensembl
  11. phospholipid binding Source: Ensembl
  12. phospholipid transporter activity Source: Ensembl
  13. protein binding Source: IntAct

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: Ensembl
  2. adrenal gland development Source: MGI
  3. blood vessel endothelial cell migration Source: MGI
  4. cholesterol biosynthetic process Source: MGI
  5. cholesterol efflux Source: MGI
  6. cholesterol homeostasis Source: Ensembl
  7. cholesterol import Source: Ensembl
  8. cholesterol metabolic process Source: MGI
  9. cholesterol transport Source: MGI
  10. endothelial cell proliferation Source: MGI
  11. glucocorticoid metabolic process Source: MGI
  12. high-density lipoprotein particle assembly Source: Ensembl
  13. lipid storage Source: MGI
  14. lipoprotein biosynthetic process Source: MGI
  15. negative regulation of cell adhesion molecule production Source: Ensembl
  16. negative regulation of cytokine secretion involved in immune response Source: Ensembl
  17. negative regulation of heterotypic cell-cell adhesion Source: Ensembl
  18. negative regulation of hydrolase activity Source: MGI
  19. negative regulation of inflammatory response Source: Ensembl
  20. negative regulation of interleukin-1 beta secretion Source: Ensembl
  21. negative regulation of lipase activity Source: Ensembl
  22. negative regulation of tumor necrosis factor-mediated signaling pathway Source: Ensembl
  23. negative regulation of very-low-density lipoprotein particle remodeling Source: Ensembl
  24. organ regeneration Source: Ensembl
  25. peptidyl-methionine modification Source: UniProtKB
  26. phosphatidylcholine biosynthetic process Source: Ensembl
  27. phospholipid efflux Source: Ensembl
  28. phospholipid homeostasis Source: Ensembl
  29. phospholipid metabolic process Source: MGI
  30. positive regulation of cholesterol esterification Source: Ensembl
  31. positive regulation of hydrolase activity Source: Ensembl
  32. positive regulation of transferase activity Source: MGI
  33. protein oxidation Source: UniProtKB
  34. protein stabilization Source: Ensembl
  35. regulation of Cdc42 protein signal transduction Source: Ensembl
  36. regulation of intestinal cholesterol absorption Source: MGI
  37. regulation of protein phosphorylation Source: MGI
  38. response to drug Source: Ensembl
  39. response to estrogen Source: Ensembl
  40. response to nutrient Source: Ensembl
  41. reverse cholesterol transport Source: Ensembl
  42. triglyceride homeostasis Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Lipid transport, Steroid metabolism, Sterol metabolism, Transport

Enzyme and pathway databases

ReactomeiREACT_196575. Scavenging of heme from plasma.
REACT_196581. Scavenging by Class A Receptors.
REACT_196592. Scavenging by Class B Receptors.
REACT_197984. ABCA transporters in lipid homeostasis.
REACT_198563. Amyloids.
REACT_198569. Retinoid metabolism and transport.
REACT_198602. PPARA activates gene expression.
REACT_213857. HDL-mediated lipid transport.
REACT_216017. Chylomicron-mediated lipid transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein A-I
Short name:
Apo-AI
Short name:
ApoA-I
Alternative name(s):
Apolipoprotein A1
Cleaved into the following 2 chains:
Gene namesi
Name:Apoa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:88049. Apoa1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. endocytic vesicle Source: Ensembl
  3. extracellular region Source: MGI
  4. extracellular space Source: MGI
  5. nucleus Source: Ensembl
  6. spherical high-density lipoprotein particle Source: Ensembl
  7. very-low-density lipoprotein particle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 By similarity
Add
BLAST
Chaini19 – 264246Proapolipoprotein A-I
PRO_0000425326Add
BLAST
Chaini25 – 264240Apolipoprotein A-I
PRO_0000001946Add
BLAST
Chaini25 – 263239Truncated apolipoprotein A-I By similarity
PRO_0000416576Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei109 – 1091Methionine sulfoxide By similarity
Modified residuei193 – 1931Methionine sulfoxide
Modified residuei240 – 2401Methionine sulfoxide
Modified residuei242 – 2421Methionine sulfoxide

Post-translational modificationi

Glycosylated By similarity.
Palmitoylated By similarity.
May be acylated.
Phosphorylation sites are present in the extracellular medium By similarity.

Keywords - PTMi

Glycoprotein, Lipoprotein, Oxidation, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ00623.
PaxDbiQ00623.
PRIDEiQ00623.

2D gel databases

REPRODUCTION-2DPAGEIPI00121209.
Q00623.
SWISS-2DPAGEQ00623.

PTM databases

PhosphoSiteiQ00623.

Expressioni

Tissue specificityi

Major protein of plasma HDL, also found in chylomicrons.

Gene expression databases

CleanExiMM_APOA1.
GenevestigatoriQ00623.

Interactioni

Subunit structurei

Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1 By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Kcnma1Q084604EBI-1634106,EBI-1633915

Protein-protein interaction databases

IntActiQ00623. 5 interactions.
MINTiMINT-1855786.
STRINGi10090.ENSMUSP00000034588.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 7142
Turni72 – 754
Helixi83 – 11937
Turni120 – 1234
Helixi126 – 14116
Helixi145 – 15410
Helixi167 – 20640
Beta strandi217 – 2193
Helixi220 – 2267
Helixi233 – 2375

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LEMNMR-A25-240[»]
ProteinModelPortaliQ00623.
SMRiQ00623. Positions 25-240.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati67 – 88221
Add
BLAST
Repeati89 – 110222
Add
BLAST
Repeati111 – 121113; half-length
Add
BLAST
Repeati122 – 143224
Add
BLAST
Repeati144 – 165225
Add
BLAST
Repeati166 – 187226
Add
BLAST
Repeati188 – 207207; truncated
Add
BLAST
Repeati208 – 229228
Add
BLAST
Repeati230 – 240119; half-length
Add
BLAST
Repeati241 – 2642410
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 26419810 X approximate tandem repeats
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG39720.
GeneTreeiENSGT00530000063081.
HOGENOMiHOG000033998.
HOVERGENiHBG105708.
InParanoidiQ00623.
KOiK08757.
OMAiYRQKVAP.
OrthoDBiEOG7TBC3N.
TreeFamiTF334458.

Family and domain databases

InterProiIPR000074. ApoA1_A4_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00623-1 [UniParc]FASTAAdd to Basket

« Hide

MKAVVLAVAL VFLTGSQAWH VWQQDEPQSQ WDKVKDFANV YVDAVKDSGR    50
DYVSQFESSS LGQQLNLNLL ENWDTLGSTV SQLQERLGPL TRDFWDNLEK 100
ETDWVRQEMN KDLEEVKQKV QPYLDEFQKK WKEDVELYRQ KVAPLGAELQ 150
ESARQKLQEL QGRLSPVAEE FRDRMRTHVD SLRTQLAPHS EQMRESLAQR 200
LAELKSNPTL NEYHTRAKTH LKTLGEKARP ALEDLRHSLM PMLETLKTQV 250
QSVIDKASET LTAQ 264
Length:264
Mass (Da):30,616
Last modified:October 3, 2012 - v2
Checksum:i5E33FF201963583B
GO

Mass spectrometryi

Molecular mass is 27951.3±1.343 Da from positions 25 - 264. Determined by ESI. Strain C57BL/6. Without methionine sulfoxide.1 Publication
Molecular mass is 27923.8 Da from positions 25 - 264. Determined by ESI. Strain BALB/c. Without methionine sulfoxide.1 Publication
Molecular mass is 27965 Da from positions 25 - 264. Determined by ESI. Strain C57BL/6. With 1 methionine sulfoxide.1 Publication
Molecular mass is 28819.7 Da from positions 19 - 264. Determined by ESI. Strain C57BL/6. Without methionine sulfoxide.1 Publication
Molecular mass is 28790.7 Da from positions 19 - 264. Determined by ESI. Strain BALB/c. Without methionine sulfoxide.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti249 – 2502QV → KA in strain: BALB/c, C3H and ICR.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti143 – 1431A → G in AAB58424. 1 Publication
Sequence conflicti143 – 1431A → G in AAB58425. 1 Publication
Sequence conflicti143 – 1431A → G in AAB58426. 1 Publication
Sequence conflicti143 – 1431A → G in AAB58427. 1 Publication
Sequence conflicti146 – 1461Missing in AAB58424. 1 Publication
Sequence conflicti146 – 1461Missing in AAB58425. 1 Publication
Sequence conflicti146 – 1461Missing in AAB58426. 1 Publication
Sequence conflicti146 – 1461Missing in AAB58427. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64262 mRNA. Translation: CAA45560.1.
X64263 Genomic DNA. Translation: CAA45561.1.
L04149 Genomic DNA. No translation available.
L04151 mRNA. No translation available.
U79572 Genomic DNA. Translation: AAB58424.1.
U79574 Genomic DNA. Translation: AAB58426.1.
U79573 Genomic DNA. Translation: AAB58425.1.
U79575 Genomic DNA. Translation: AAB58427.1.
AK076187 mRNA. Translation: BAC36241.1.
AK149576 mRNA. Translation: BAE28968.1.
AK161536 mRNA. Translation: BAE36448.1.
AC116503 Genomic DNA. No translation available.
BC012253 mRNA. Translation: AAH12253.1.
BC019837 mRNA. Translation: AAH19837.1.
CCDSiCCDS40610.1.
PIRiS22420.
RefSeqiNP_033822.2. NM_009692.4.
UniGeneiMm.26743.

Genome annotation databases

EnsembliENSMUST00000034588; ENSMUSP00000034588; ENSMUSG00000032083.
GeneIDi11806.
KEGGimmu:11806.
UCSCiuc009phb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64262 mRNA. Translation: CAA45560.1 .
X64263 Genomic DNA. Translation: CAA45561.1 .
L04149 Genomic DNA. No translation available.
L04151 mRNA. No translation available.
U79572 Genomic DNA. Translation: AAB58424.1 .
U79574 Genomic DNA. Translation: AAB58426.1 .
U79573 Genomic DNA. Translation: AAB58425.1 .
U79575 Genomic DNA. Translation: AAB58427.1 .
AK076187 mRNA. Translation: BAC36241.1 .
AK149576 mRNA. Translation: BAE28968.1 .
AK161536 mRNA. Translation: BAE36448.1 .
AC116503 Genomic DNA. No translation available.
BC012253 mRNA. Translation: AAH12253.1 .
BC019837 mRNA. Translation: AAH19837.1 .
CCDSi CCDS40610.1.
PIRi S22420.
RefSeqi NP_033822.2. NM_009692.4.
UniGenei Mm.26743.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LEM NMR - A 25-240 [» ]
ProteinModelPortali Q00623.
SMRi Q00623. Positions 25-240.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q00623. 5 interactions.
MINTi MINT-1855786.
STRINGi 10090.ENSMUSP00000034588.

PTM databases

PhosphoSitei Q00623.

2D gel databases

REPRODUCTION-2DPAGE IPI00121209.
Q00623.
SWISS-2DPAGE Q00623.

Proteomic databases

MaxQBi Q00623.
PaxDbi Q00623.
PRIDEi Q00623.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034588 ; ENSMUSP00000034588 ; ENSMUSG00000032083 .
GeneIDi 11806.
KEGGi mmu:11806.
UCSCi uc009phb.2. mouse.

Organism-specific databases

CTDi 335.
MGIi MGI:88049. Apoa1.

Phylogenomic databases

eggNOGi NOG39720.
GeneTreei ENSGT00530000063081.
HOGENOMi HOG000033998.
HOVERGENi HBG105708.
InParanoidi Q00623.
KOi K08757.
OMAi YRQKVAP.
OrthoDBi EOG7TBC3N.
TreeFami TF334458.

Enzyme and pathway databases

Reactomei REACT_196575. Scavenging of heme from plasma.
REACT_196581. Scavenging by Class A Receptors.
REACT_196592. Scavenging by Class B Receptors.
REACT_197984. ABCA transporters in lipid homeostasis.
REACT_198563. Amyloids.
REACT_198569. Retinoid metabolism and transport.
REACT_198602. PPARA activates gene expression.
REACT_213857. HDL-mediated lipid transport.
REACT_216017. Chylomicron-mediated lipid transport.

Miscellaneous databases

ChiTaRSi APOA1. mouse.
NextBioi 279667.
PROi Q00623.
SOURCEi Search...

Gene expression databases

CleanExi MM_APOA1.
Genevestigatori Q00623.

Family and domain databases

InterProi IPR000074. ApoA1_A4_E.
[Graphical view ]
Pfami PF01442. Apolipoprotein. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse apolipoprotein AI. cDNA-derived primary structure, gene organisation and complete nucleotide sequence."
    Stoffel W., Mueller R., Binczek E., Hofmann K.
    Biol. Chem. Hoppe-Seyler 373:187-193(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "Characterization of the mouse apolipoprotein Apoa-1/Apoc-3 gene locus: genomic, mRNA, and protein sequences with comparisons to other species."
    Januzzi J.L., Azrolan N., O'Connell A., Aalto-Setala K., Breslow J.L.
    Genomics 14:1081-1088(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. Chiang A.-N., Fan K.-C., Shaw G.-C., Yang U.-C.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT 249-LYS-ALA--250.
    Strain: BALB/c, C3H, C57BL/6 and ICR.
    Tissue: Spleen.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT 249-GLN-VAL-250.
    Strain: C57BL/6J.
    Tissue: Liver, Ovary, Placenta and Uterus.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT 249-GLN-VAL-250.
    Strain: C57BL/6J.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  7. "Mass spectral analysis of the apolipoproteins on mouse high density lipoproteins. Detection of post-translational modifications."
    Puppione D.L., Yam L.M., Bassilian S., Souda P., Castellani L.W., Schumaker V.N., Whitelegge J.P.
    Biochim. Biophys. Acta 1764:1363-1371(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-46; 93-106; 120-129; 131-139; 142-154; 157-172; 177-194; 201-216 AND 223-256, MASS SPECTROMETRY, OXIDATION AT MET-193; MET-240 AND MET-242 TO METHIONINE SULFOXIDES.

Entry informationi

Entry nameiAPOA1_MOUSE
AccessioniPrimary (citable) accession number: Q00623
Secondary accession number(s): O08855, O09042, Q8BPD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 3, 2012
Last modified: September 3, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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