Q00619 (MAM2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pheromone P-factor receptor

Gene names

Name:	mam2
ORF Names:	SPAC11H11.04

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Receptor for the peptide pheromone P-factor, a mating factor of S.pombe. Pheromone signaling is essential for initiation of meiosis in S.pombe; P-factor signaling alone may be sufficient.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 4 family.

Ontologies

Keywords
Biological process	Pheromone response
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of signal transduction in absence of ligand Inferred from mutant phenotype PubMed 15659165. Source: PomBase pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from mutant phenotype PubMed 15659165Ref.1. Source: PomBase positive regulation of conjugation with cellular fusion Inferred from mutant phenotype PubMed 15659165. Source: PomBase positive regulation of diacylglycerol biosynthetic process Inferred from mutant phenotype PubMed 7672243. Source: PomBase positive regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from mutant phenotype PubMed 15580593. Source: PomBase protein homooligomerization Inferred from experiment PubMed 15659165. Source: PomBase receptor transactivation Inferred from direct assay PubMed 15659165. Source: PomBase
Cellular_component	G-protein coupled receptor homodimeric complex Inferred from direct assay PubMed 15659165. Source: PomBase cell division site Inferred from direct assay PubMed 16823372. Source: PomBase cell tip Inferred from direct assay PubMed 16823372. Source: PomBase fungal-type vacuole Inferred from direct assay PubMed 16823372. Source: PomBase integral to plasma membrane Inferred from direct assay PubMed 15659165. Source: PomBase
Molecular_function	mating-type P-factor pheromone receptor activity Inferred from direct assay PubMed 15659165. Source: PomBase pheromone binding Inferred from mutant phenotype Ref.1. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Pheromone P-factor receptor		PRO_0000195074
Regions
	Transmembrane	46 – 69	24	Helical; Name=1; Potential
	Transmembrane	79 – 103	25	Helical; Name=2; Potential
	Transmembrane	125 – 141	17	Helical; Name=3; Potential
	Transmembrane	162 – 180	19	Helical; Name=4; Potential
	Transmembrane	207 – 225	19	Helical; Name=5; Potential
	Transmembrane	249 – 267	19	Helical; Name=6; Potential
	Transmembrane	283 – 301	19	Helical; Name=7; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q00619 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 3235DE5E3E513EB0	FASTA	348	39,286
	10 20 30 40 50 60 MRQPWWKDFT IPDASAIIHQ NITIVSIVGE IEVPVSTIDA YERDRLLTGM TLSAQLALGV 70 80 90 100 110 120 LTILMVCLLS SSEKRKHPVF VFNSASIVAM CLRAILNIVT ICSNSYSILV NYGFILNMVH 130 140 150 160 170 180 MYVHVFNILI LLLAPVIIFT AEMSMMIQVR IICAHDRKTQ RIMTVISACL TVLVLAFWIT 190 200 210 220 230 240 NMCQQIQYLL WLTPLSSKTI VGYSWPYFIA KILFAFSIIF HSGVFSYKLF RAILIRKKIG 250 260 270 280 290 300 QFPFGPMQCI LVISCQCLIV PATFTIIDSF IHTYDGFSSM TQCLLIISLP LSSLWASSTA 310 320 330 340 LKLQSMKTSS AQGETTEVSI RVDRTFDIKH TPSDDYSISD ESETKKWT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Schizosaccharomyces pombe mam2 gene encodes a putative pheromone receptor which has a significant homology with the Saccharomyces cerevisiae Ste2 protein." Kitamura K., Shimoda C. EMBO J. 10:3743-3751(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38365 / 975.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61672 Genomic DNA. Translation: CAA43846.1. CU329670 Genomic DNA. Translation: CAB59800.1.
PIR	S18521.
RefSeq	NP_594722.1. NM_001020150.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4693314.
STRING	4896.SPAC11H11.04-1.
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC11H11.04.1; SPAC11H11.04.1:pep; SPAC11H11.04.
GeneID	2541606.
KEGG	spo:SPAC11H11.04.
Organism-specific databases
PomBase	SPAC11H11.04.
Phylogenomic databases
eggNOG	NOG68014.
KO	K11213.
OMA	IFIMGCQ.
OrthoDB	EOG42592C.
Family and domain databases
Gene3D	1.10.287.920. 1 hit.
InterPro	IPR000366. GPCR_STE2. IPR027458. STE2_TM1-TM2. [Graphical view]
Pfam	PF02116. STE2. 1 hit. [Graphical view]
PRINTS	PR00250. GPCRSTE2.
ProtoNet	Search...
Other
NextBio	20802700.

Entry information

Entry name

MAM2_SCHPO

Accession

Primary (citable) accession number: Q00619

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 29, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program