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Q00618 (PGTA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Geranylgeranyl transferase type-2 subunit alpha

EC=2.5.1.60
Alternative name(s):
GGTase-II-alpha
Geranylgeranyl transferase type II subunit alpha
PGGT
Type II protein geranyl-geranyltransferase subunit alpha
YPT1/SEC4 proteins geranylgeranyltransferase subunit alpha
Gene names
Name:BET4
Ordered Locus Names:YJL031C
ORF Names:J1254
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal -XCC or -XCXC, where both cysteines may become modified. Acts on YPT1 and SEC4.

Catalytic activity

Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.

Subunit structure

Heterodimer of an alpha and a beta subunit.

Sequence similarities

Belongs to the protein prenyltransferase subunit alpha family.

Contains 5 PFTA repeats.

Caution

Was originally (Ref.1) thought to be MAD2.

Sequence caution

The sequence AAA21386.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA89323.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BET2P201333EBI-3573,EBI-3559

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 327327Geranylgeranyl transferase type-2 subunit alpha
PRO_0000119756

Regions

Repeat44 – 7835PFTA 1
Repeat84 – 11835PFTA 2
Repeat123 – 15735PFTA 3
Repeat163 – 19735PFTA 4
Repeat207 – 24135PFTA 5

Sequences

Sequence LengthMass (Da)Tools
Q00618 [UniParc].

Last modified August 30, 2005. Version 3.
Checksum: A7C3E16C14DDD09C

FASTA32739,675
        10         20         30         40         50         60 
MHGIKRKQWT KELLRQKRVQ DEKKIYDYRS LTENVLNMRD EKIYSIEALK KTSELLEKNP 

        70         80         90        100        110        120 
EFNAIWNYRR DIIASLASEL EIPFWDKELV FVMMLLKDYP KVYWIWNHRL WVLKHYPTSS 

       130        140        150        160        170        180 
PKVWQTELAV VNKLLEQDAR NYHGWHYRRI VVGNIESITN KSLDKEEFEY TTIKINNNIS 

       190        200        210        220        230        240 
NYSAWHQRVQ IISRMFQKGE VGNQKEYIRT EISYIINAMF TDAEDQSVWF YIKWFIKNDI 

       250        260        270        280        290        300 
VCKTLDEQEY LKMLKDLREN ILLINNDEIE FSGKQNIWCL KILLVLEDIL EEKEALTERS 

       310        320 
SEQYLVQLID ADPLRKNRYL HLLEQHK 

« Hide

References

« Hide 'large scale' references
[1]"Feedback control of mitosis in budding yeast."
Li R., Murray A.W.
Cell 66:519-531(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Li R., Chen R.-H., Murray A.W.
Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 171.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVISION OF GENE MODEL.
Strain: ATCC 204511 / S288c / AB972.
[6]"The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase."
Li R., Havel C., Watson J.A., Murray A.W.
Nature 366:82-84(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[7]Erratum
Li R., Havel C., Watson J.A., Murray A.W.
Nature 371:438-438(1994)
[8]"Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p."
Jiang Y., Rossi G., Ferro-Novick S.
Nature 366:84-86(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U14132 Genomic DNA. Translation: AAA21386.1. Sequence problems.
Z49307 Genomic DNA. Translation: CAA89323.1. Sequence problems.
BK006943 Genomic DNA. Translation: DAA08767.1.
PIRS48301.
RefSeqNP_012503.2. NM_001181465.1.

3D structure databases

ProteinModelPortalQ00618.
SMRQ00618. Positions 2-315.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33728. 12 interactions.
DIPDIP-4994N.
IntActQ00618. 1 interaction.
MINTMINT-568965.
STRING4932.YJL031C.

Proteomic databases

PaxDbQ00618.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL031C; YJL031C; YJL031C.
GeneID853421.
KEGGsce:YJL031C.

Organism-specific databases

CYGDYJL031c.
SGDS000003568. BET4.

Phylogenomic databases

eggNOGCOG5536.
GeneTreeENSGT00550000075121.
HOGENOMHOG000190796.
KOK14050.
OMAGWHYRRI.
OrthoDBEOG77Q569.

Enzyme and pathway databases

BioCycYEAST:MONOMER3O-3.

Gene expression databases

GenevestigatorQ00618.

Family and domain databases

InterProIPR002088. Prenyl_trans_a.
[Graphical view]
PfamPF01239. PPTA. 5 hits.
[Graphical view]
PROSITEPS51147. PFTA. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio973945.
PROQ00618.

Entry information

Entry namePGTA_YEAST
AccessionPrimary (citable) accession number: Q00618
Secondary accession number(s): D6VWF1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: August 30, 2005
Last modified: February 19, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families