Reviewed, UniProtKB/Swiss-Prot Q00618 (PGTA_YEAST)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl transferase type-2 subunit alpha
    EC=2.5.1.60
Alternative name(s):
    Geranylgeranyl transferase type II subunit alpha
    Type II protein geranyl-geranyltransferase subunit alpha
    GGTase-II-alpha
    PGGT
    YPT1/SEC4 proteins geranylgeranyltransferase subunit alpha

Gene names

Name:	BET4
Ordered Locus Names:	YJL031C
ORF Names:	J1254

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal -XCC or -XCXC, where both cysteines may become modified. Acts on YPT1 and SEC4.
Catalytic activity	Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Sequence similarities	Belongs to the protein prenyltransferase subunit alpha family. Contains 5 PFTA repeats.
Caution	Was originally (Ref.1) thought to be MAD2.
Sequence caution	The sequence AAA21386.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA89323.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Repeat
Molecular function	Prenyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid geranylgeranylation Inferred from direct assay. Source: SGD
Cellular component	Rab-protein geranylgeranyltransferase complex Inferred from direct assay. Source: SGD
Molecular function	Rab geranylgeranyltransferase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BET2	P20133	1	EBI-3573,EBI-3559

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Geranylgeranyl transferase type-2 subunit alpha		PRO_0000119756
Regions
	Repeat	44 – 78	35	PFTA 1
	Repeat	84 – 118	35	PFTA 2
	Repeat	123 – 157	35	PFTA 3
	Repeat	163 – 197	35	PFTA 4
	Repeat	207 – 241	35	PFTA 5

Sequences

	Sequence		Length	Mass (Da)
	Q00618-1 [UniParc]. Last modified August 30, 2005. Version 3. Checksum: A7C3E16C14DDD09C	FASTA	327	39,675
	10 20 30 40 50 60 MHGIKRKQWT KELLRQKRVQ DEKKIYDYRS LTENVLNMRD EKIYSIEALK KTSELLEKNP 70 80 90 100 110 120 EFNAIWNYRR DIIASLASEL EIPFWDKELV FVMMLLKDYP KVYWIWNHRL WVLKHYPTSS 130 140 150 160 170 180 PKVWQTELAV VNKLLEQDAR NYHGWHYRRI VVGNIESITN KSLDKEEFEY TTIKINNNIS 190 200 210 220 230 240 NYSAWHQRVQ IISRMFQKGE VGNQKEYIRT EISYIINAMF TDAEDQSVWF YIKWFIKNDI 250 260 270 280 290 300 VCKTLDEQEY LKMLKDLREN ILLINNDEIE FSGKQNIWCL KILLVLEDIL EEKEALTERS 310 320 SEQYLVQLID ADPLRKNRYL HLLEQHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Feedback control of mitosis in budding yeast." Li R., Murray A.W. Cell 66:519-531(1991) [PubMed: 1651172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Li R., Chen R.-H., Murray A.W. Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 171.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:RESEARCH45.1-RESEARCH45.13(2003) [PubMed: 12844361] [Abstract] Cited for: REVISION OF GENE MODEL. Strain: ATCC 204511 / S288c / AB972.
[5]	"The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase." Li R., Havel C., Watson J.A., Murray A.W. Nature 366:82-84(1993) [PubMed: 8232541] [Abstract] Cited for: CHARACTERIZATION.
[6]	Erratum Li R., Havel C., Watson J.A., Murray A.W. Nature 371:438-438(1994)
[7]	"Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p." Jiang Y., Rossi G., Ferro-Novick S. Nature 366:84-86(1993) [PubMed: 8232542] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U14132 Genomic DNA. Translation: AAA21386.1. Sequence problems. Z49307 Genomic DNA. Translation: CAA89323.1. Sequence problems.
PIR	S48301.
RefSeq	NP_012503.2.
3D structure databases
HSSP	HSSP built from PDB template 1LTX based on UniProtKB Q08602.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4994N.
IntAct	Q00618. 5 interactions.
STRING	Q00618.
Genome annotation databases
Ensembl	YJL031C; YJL031C; YJL031C; Saccharomyces cerevisiae. [Genome view]
GeneID	853421.
GenomeReviews	Gene locus YJL031C in contig Y13136_GR.
KEGG	sce:YJL031C.
NMPDR	fig|4932.3.peg.3477.
Organism-specific databases
CYGD	YJL031c.
SGD	S000003568. BET4.
Phylogenomic databases
HOGENOM	Q00618.
OMA	WNYRRDI.
Enzyme and pathway databases
BRENDA	2.5.1.60. 250.
Gene expression databases
ArrayExpress	Q00618.
Genevestigator	Q00618.
GermOnline	YJL031C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002088. Prenyl_trans_a. IPR008940. Prenyltransferase. [Graphical view]
Gene3D	G3DSA:1.25.40.120. Prenyl_trans. 1 hit.
Pfam	PF01239. PPTA. 5 hits. [Graphical view]
PROSITE	PS51147. PFTA. 5 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973945.

Entry information

Entry name

PGTA_YEAST

Accession

Primary (citable) accession number: Q00618

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	August 30, 2005
Last modified:	November 3, 2009
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)