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Q00618

- PGTA_YEAST

UniProt

Q00618 - PGTA_YEAST

Protein

Geranylgeranyl transferase type-2 subunit alpha

Gene

BET4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 3 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal -XCC or -XCXC, where both cysteines may become modified. Acts on YPT1 and SEC4.

    Catalytic activityi

    Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. Rab geranylgeranyltransferase activity Source: SGD
    3. Rab GTPase binding Source: UniProtKB

    GO - Biological processi

    1. ER to Golgi vesicle-mediated transport Source: SGD
    2. protein geranylgeranylation Source: SGD
    3. protein targeting to membrane Source: SGD

    Keywords - Molecular functioni

    Prenyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:MONOMER3O-3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Geranylgeranyl transferase type-2 subunit alpha (EC:2.5.1.60)
    Alternative name(s):
    GGTase-II-alpha
    Geranylgeranyl transferase type II subunit alpha
    PGGT
    Type II protein geranyl-geranyltransferase subunit alpha
    YPT1/SEC4 proteins geranylgeranyltransferase subunit alpha
    Gene namesi
    Name:BET4
    Ordered Locus Names:YJL031C
    ORF Names:J1254
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL031c.
    SGDiS000003568. BET4.

    Subcellular locationi

    GO - Cellular componenti

    1. Rab-protein geranylgeranyltransferase complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Geranylgeranyl transferase type-2 subunit alphaPRO_0000119756Add
    BLAST

    Proteomic databases

    MaxQBiQ00618.
    PaxDbiQ00618.

    Expressioni

    Gene expression databases

    GenevestigatoriQ00618.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BET2P201333EBI-3573,EBI-3559

    Protein-protein interaction databases

    BioGridi33728. 12 interactions.
    DIPiDIP-4994N.
    IntActiQ00618. 1 interaction.
    MINTiMINT-568965.
    STRINGi4932.YJL031C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00618.
    SMRiQ00618. Positions 2-315.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati44 – 7835PFTA 1Add
    BLAST
    Repeati84 – 11835PFTA 2Add
    BLAST
    Repeati123 – 15735PFTA 3Add
    BLAST
    Repeati163 – 19735PFTA 4Add
    BLAST
    Repeati207 – 24135PFTA 5Add
    BLAST

    Sequence similaritiesi

    Contains 5 PFTA repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5536.
    GeneTreeiENSGT00550000075121.
    HOGENOMiHOG000190796.
    KOiK14050.
    OMAiGWHYRRI.
    OrthoDBiEOG77Q569.

    Family and domain databases

    InterProiIPR002088. Prenyl_trans_a.
    [Graphical view]
    PfamiPF01239. PPTA. 5 hits.
    [Graphical view]
    PROSITEiPS51147. PFTA. 5 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00618-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHGIKRKQWT KELLRQKRVQ DEKKIYDYRS LTENVLNMRD EKIYSIEALK    50
    KTSELLEKNP EFNAIWNYRR DIIASLASEL EIPFWDKELV FVMMLLKDYP 100
    KVYWIWNHRL WVLKHYPTSS PKVWQTELAV VNKLLEQDAR NYHGWHYRRI 150
    VVGNIESITN KSLDKEEFEY TTIKINNNIS NYSAWHQRVQ IISRMFQKGE 200
    VGNQKEYIRT EISYIINAMF TDAEDQSVWF YIKWFIKNDI VCKTLDEQEY 250
    LKMLKDLREN ILLINNDEIE FSGKQNIWCL KILLVLEDIL EEKEALTERS 300
    SEQYLVQLID ADPLRKNRYL HLLEQHK 327
    Length:327
    Mass (Da):39,675
    Last modified:August 30, 2005 - v3
    Checksum:iA7C3E16C14DDD09C
    GO

    Sequence cautioni

    The sequence AAA21386.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA89323.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14132 Genomic DNA. Translation: AAA21386.1. Sequence problems.
    Z49307 Genomic DNA. Translation: CAA89323.1. Sequence problems.
    BK006943 Genomic DNA. Translation: DAA08767.1.
    PIRiS48301.
    RefSeqiNP_012503.2. NM_001181465.1.

    Genome annotation databases

    EnsemblFungiiYJL031C; YJL031C; YJL031C.
    GeneIDi853421.
    KEGGisce:YJL031C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14132 Genomic DNA. Translation: AAA21386.1 . Sequence problems.
    Z49307 Genomic DNA. Translation: CAA89323.1 . Sequence problems.
    BK006943 Genomic DNA. Translation: DAA08767.1 .
    PIRi S48301.
    RefSeqi NP_012503.2. NM_001181465.1.

    3D structure databases

    ProteinModelPortali Q00618.
    SMRi Q00618. Positions 2-315.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33728. 12 interactions.
    DIPi DIP-4994N.
    IntActi Q00618. 1 interaction.
    MINTi MINT-568965.
    STRINGi 4932.YJL031C.

    Proteomic databases

    MaxQBi Q00618.
    PaxDbi Q00618.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL031C ; YJL031C ; YJL031C .
    GeneIDi 853421.
    KEGGi sce:YJL031C.

    Organism-specific databases

    CYGDi YJL031c.
    SGDi S000003568. BET4.

    Phylogenomic databases

    eggNOGi COG5536.
    GeneTreei ENSGT00550000075121.
    HOGENOMi HOG000190796.
    KOi K14050.
    OMAi GWHYRRI.
    OrthoDBi EOG77Q569.

    Enzyme and pathway databases

    BioCyci YEAST:MONOMER3O-3.

    Miscellaneous databases

    NextBioi 973945.
    PROi Q00618.

    Gene expression databases

    Genevestigatori Q00618.

    Family and domain databases

    InterProi IPR002088. Prenyl_trans_a.
    [Graphical view ]
    Pfami PF01239. PPTA. 5 hits.
    [Graphical view ]
    PROSITEi PS51147. PFTA. 5 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Feedback control of mitosis in budding yeast."
      Li R., Murray A.W.
      Cell 66:519-531(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Li R., Chen R.-H., Murray A.W.
      Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 171.
    3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
      Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
      Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVISION OF GENE MODEL.
      Strain: ATCC 204511 / S288c / AB972.
    6. "The mitotic feedback control gene MAD2 encodes the alpha-subunit of a prenyltransferase."
      Li R., Havel C., Watson J.A., Murray A.W.
      Nature 366:82-84(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. Erratum
      Li R., Havel C., Watson J.A., Murray A.W.
      Nature 371:438-438(1994)
    8. "Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p."
      Jiang Y., Rossi G., Ferro-Novick S.
      Nature 366:84-86(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiPGTA_YEAST
    AccessioniPrimary (citable) accession number: Q00618
    Secondary accession number(s): D6VWF1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 122 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be MAD2.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3