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Q00612 (G6PD1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase X

Short name=G6PD
EC=1.1.1.49
Gene names
Name:G6pdx
Synonyms:G6pd, G6pd-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis By similarity. HAMAP-Rule MF_00966

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. HAMAP-Rule MF_00966

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Subunit structure

Homotetramer; dimer of dimers By similarity. HAMAP-Rule MF_00966

Miscellaneous

Has NADP both as cofactor (bound to the N-terminal domain) and as structural element bound to the C-terminal domain.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
   LigandNADP
   Molecular functionOxidoreductase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processNADP biosynthetic process

Inferred from direct assay PubMed 7439685PubMed 9169132Ref.5PubMed 9539108PubMed 9627357. Source: MGI

NADP metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure

Inferred from mutant phenotype PubMed 15998684. Source: MGI

cytokine production

Inferred from mutant phenotype PubMed 15817708. Source: MGI

erythrocyte development

Inferred from mutant phenotype PubMed 14751857PubMed 15271799. Source: MGI

glucose 6-phosphate metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

glutathione metabolic process

Inferred from mutant phenotype PubMed 12521604. Source: MGI

interleukin-10 production

Inferred from mutant phenotype PubMed 15817708. Source: MGI

interleukin-12 production

Inferred from mutant phenotype PubMed 15817708. Source: MGI

pentose biosynthetic process

Inferred from direct assay PubMed 7439685PubMed 8491670Ref.5PubMed 9539108PubMed 9627357. Source: MGI

pentose-phosphate shunt

Inferred from electronic annotation. Source: UniProtKB-UniPathway

regulation of multicellular organism growth

Inferred from mutant phenotype PubMed 10627286. Source: MGI

response to oxidative stress

Inferred from mutant phenotype PubMed 10627286PubMed 14751857PubMed 14757696PubMed 15817708PubMed 15998684PubMed 7489710. Source: MGI

vasodilation by angiotensin involved in regulation of systemic arterial blood pressure

Inferred from mutant phenotype PubMed 15998684. Source: MGI

   Molecular_functionNADP binding

Inferred from electronic annotation. Source: InterPro

glucose-6-phosphate dehydrogenase activity

Inferred from direct assay PubMed 12521604PubMed 14757696PubMed 15155459PubMed 15998684PubMed 3243423PubMed 5764873PubMed 7439685PubMed 8491670PubMed 9169132Ref.5PubMed 943046PubMed 9539108PubMed 9627357. Source: MGI

protein homodimerization activity

Inferred from physical interaction PubMed 9169132. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 515514Glucose-6-phosphate 1-dehydrogenase X HAMAP-Rule MF_00966
PRO_0000068085

Regions

Nucleotide binding38 – 458NADP 1 By similarity
Nucleotide binding401 – 4033NADP 2 By similarity
Nucleotide binding421 – 4233NADP 2 By similarity
Region201 – 2055Substrate binding By similarity

Sites

Active site2631Proton acceptor By similarity
Binding site721NADP 1 By similarity
Binding site1471NADP 1 By similarity
Binding site1711NADP 1; via carbonyl oxygen By similarity
Binding site1711Substrate By similarity
Binding site2391Substrate By similarity
Binding site2581Substrate By similarity
Binding site3571NADP 2 By similarity
Binding site3601Substrate By similarity
Binding site3651Substrate By similarity
Binding site3661NADP 2 By similarity
Binding site3701NADP 2 By similarity
Binding site3931NADP 2 By similarity
Binding site3951Substrate By similarity
Binding site4871NADP 2 By similarity
Binding site5031NADP 2 By similarity
Binding site5091NADP 2 By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue5071Phosphotyrosine Ref.6

Experimental info

Sequence conflict241D → Y in CAA37679. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q00612 [UniParc].

Last modified March 20, 2007. Version 3.
Checksum: C8AF0D4099B7AEC3

FASTA51559,263
        10         20         30         40         50         60 
MAEQVALSRT QVCGILREEL YQGDAFHQAD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL 

        70         80         90        100        110        120 
LPEDTFIVGY ARSRLTVDDI RKQSEPFFKA TPEERPKLEE FFARNSYVAG QYDDAASYKH 

       130        140        150        160        170        180 
LNSHMNALHQ GMQANRLFYL ALPPTVYEAV TKNIQETCMS QTGWNRIIVE KPFGRDLQSS 

       190        200        210        220        230        240 
NQLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP 

       250        260        270        280        290        300 
FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPATTG SDDVRDEKVK VLKCISEVET 

       310        320        330        340        350        360 
DNVVLGQYVG NPNGEGEAAN GYLDDPTVPH GSTTATFAAA VLYVENERWD GVPFILRCGK 

       370        380        390        400        410        420 
ALNERKAEVR LQFRDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL 

       430        440        450        460        470        480 
DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH KIDREKPQPI 

       490        500        510 
PYVYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of mouse glucose-6-phosphate dehydrogenase cDNA."
Zollo M.Z., D'Urso M., Schlessinger D., Chen E.Y.
DNA Seq. 3:319-322(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NOD.
Tissue: Thymus.
[3]"The CpG island in the 5' region of the G6PD gene of man and mouse."
Toniolo D., Filippi M., Dono R., Lettieri T., Martini G.
Gene 102:197-203(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
Strain: BALB/c.
[4]Lubec G., Klug S., Yang J.W., Zigmond M.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 57-71; 175-191 AND 247-257, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Brain and Hippocampus.
[5]"Somatic mutation of the glucose-6-phosphate dehydrogenase (g6pd) gene in colonic stem cells and crypt restricted loss of G6PD activity."
Kuraguchi M., Thomas G.A., Williams E.D.
Mutat. Res. 379:69-75(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 163-214 AND 352-454.
Strain: BALB/c.
[6]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-507, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11911 mRNA. Translation: CAA77967.1.
AK088135 mRNA. Translation: BAC40166.1.
X53617 Genomic DNA. Translation: CAA37679.1.
U88533 Genomic DNA. Translation: AAB52998.1.
U88534 Genomic DNA. Translation: AAB52999.1.
PIRA56686.
RefSeqNP_032088.1. NM_008062.2.
UniGeneMm.27210.

3D structure databases

ProteinModelPortalQ00612.
SMRQ00612. Positions 28-515.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ00612. 3 interactions.
MINTMINT-4095477.

PTM databases

PhosphoSiteQ00612.

2D gel databases

REPRODUCTION-2DPAGEIPI00228385.
Q00612.

Proteomic databases

PaxDbQ00612.
PRIDEQ00612.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000004327; ENSMUSP00000004327; ENSMUSG00000031400.
GeneID14381.
KEGGmmu:14381.
UCSCuc009toy.1. mouse.

Organism-specific databases

CTD14381.
MGIMGI:105979. G6pdx.

Phylogenomic databases

eggNOGCOG0364.
HOGENOMHOG000046192.
HOVERGENHBG000856.
InParanoidQ00612.
KOK00036.
OMAAVVFKRA.
OrthoDBEOG7DRJ2T.
PhylomeDBQ00612.
TreeFamTF300584.

Enzyme and pathway databases

UniPathwayUPA00115; UER00408.

Gene expression databases

ArrayExpressQ00612.
BgeeQ00612.
CleanExMM_G6PDX.
GenevestigatorQ00612.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFPIRSF000110. G6PD. 1 hit.
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio285893.
PROQ00612.
SOURCESearch...

Entry information

Entry nameG6PD1_MOUSE
AccessionPrimary (citable) accession number: Q00612
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: March 20, 2007
Last modified: April 16, 2014
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot