Reviewed, UniProtKB/Swiss-Prot Q00612 (G6PD1_MOUSE)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate 1-dehydrogenase X
      Short name=G6PD
    EC=1.1.1.49

Gene names

Name:	G6pdx
Synonyms:	G6pd, G6pd-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate + NADP(+) = D-glucono-1,5-lactone 6-phosphate + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Miscellaneous	Has NADP both as cofactor (bound to the N-terminal domain) and as structural element bound to the C-terminal domain.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	NADP biosynthetic process Ref.5 Inferred from direct assay. Source: MGI angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure Inferred from mutant phenotype. Source: MGI erythrocyte development Inferred from mutant phenotype. Source: MGI glucose metabolic process Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from mutant phenotype. Source: MGI interleukin-10 production Inferred from mutant phenotype. Source: MGI interleukin-12 production Inferred from mutant phenotype. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose biosynthetic process Ref.5 Inferred from direct assay. Source: MGI regulation of multicellular organism growth Inferred from mutant phenotype. Source: MGI response to oxidative stress Inferred from mutant phenotype. Source: MGI vasodilation by angiotensin involved in regulation of systemic arterial blood pressure Inferred from mutant phenotype. Source: MGI
Molecular function	glucose-6-phosphate dehydrogenase activity Ref.5 Inferred from direct assay. Source: MGI protein homodimerization activity Inferred from physical interaction. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 515	514	Glucose-6-phosphate 1-dehydrogenase X		PRO_0000068085
Sites
	Active site	263	1	Proton acceptor By similarity
	Binding site	40	1	NADP By similarity
	Binding site	72	1	NADP By similarity
	Binding site	201	1	Substrate By similarity
	Binding site	205	1	Substrate By similarity
	Binding site	238	1	NADP By similarity
	Binding site	357	1	NADP By similarity
	Binding site	487	1	NADP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	401	1	Phosphotyrosine
	Modified residue	503	1	Phosphotyrosine
	Modified residue	507	1	Phosphotyrosine
Experimental info
	Sequence conflict	24	1	D → Y in CAA37679. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q00612-1 [UniParc]. Last modified March 20, 2007. Version 3. Checksum: C8AF0D4099B7AEC3	FASTA	515	59,263
	10 20 30 40 50 60 MAEQVALSRT QVCGILREEL YQGDAFHQAD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL 70 80 90 100 110 120 LPEDTFIVGY ARSRLTVDDI RKQSEPFFKA TPEERPKLEE FFARNSYVAG QYDDAASYKH 130 140 150 160 170 180 LNSHMNALHQ GMQANRLFYL ALPPTVYEAV TKNIQETCMS QTGWNRIIVE KPFGRDLQSS 190 200 210 220 230 240 NQLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP 250 260 270 280 290 300 FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPATTG SDDVRDEKVK VLKCISEVET 310 320 330 340 350 360 DNVVLGQYVG NPNGEGEAAN GYLDDPTVPH GSTTATFAAA VLYVENERWD GVPFILRCGK 370 380 390 400 410 420 ALNERKAEVR LQFRDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL 430 440 450 460 470 480 DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH KIDREKPQPI 490 500 510 PYVYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of mouse glucose-6-phosphate dehydrogenase cDNA." Zollo M.Z., D'Urso M., Schlessinger D., Chen E.Y. DNA Seq. 3:319-322(1993) [PubMed: 8400362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD. Tissue: Thymus.
[3]	"The CpG island in the 5' region of the G6PD gene of man and mouse." Toniolo D., Filippi M., Dono R., Lettieri T., Martini G. Gene 102:197-203(1991) [PubMed: 1874446] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40. Strain: BALB/c.
[4]	Lubec G., Klug S., Yang J.W., Zigmond M. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 57-71; 175-191 AND 247-257, MASS SPECTROMETRY. Tissue: Brain and Hippocampus.
[5]	"Somatic mutation of the glucose-6-phosphate dehydrogenase (g6pd) gene in colonic stem cells and crypt restricted loss of G6PD activity." Kuraguchi M., Thomas G.A., Williams E.D. Mutat. Res. 379:69-75(1997) [PubMed: 9330624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 163-214 AND 352-454. Strain: BALB/c.
[6]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-507, MASS SPECTROMETRY. Tissue: Mast cell.
[7]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-401; TYR-503 AND TYR-507, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z11911 mRNA. Translation: CAA77967.1. AK088135 mRNA. Translation: BAC40166.1. X53617 Genomic DNA. Translation: CAA37679.1. U88533 Genomic DNA. Translation: AAB52998.1. U88534 Genomic DNA. Translation: AAB52999.1.
PIR	A56686.
RefSeq	NP_032088.1.
UniGene	Mm.27210
3D structure databases
HSSP	HSSP built from PDB template 1QKI based on UniProtKB P11413.
SMR	Q00612. Positions 28-515.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q00612.
PTM databases
PhosphoSite	Q00612.
2-D gel databases
REPRODUCTION-2DPAGE	Q00612.
Genome annotation databases
Ensembl	ENSMUSG00000031400. Mus musculus. [Contig view]
GeneID	14381.
KEGG	mmu:14381.
Organism-specific databases
MGI	MGI:105979. G6pdx.
Phylogenomic databases
HOGENOM	Q00612.
HOVERGEN	Q00612.
Gene expression databases
ArrayExpress	Q00612.
CleanEx	MM_G6PDX.
GermOnline	ENSMUSG00000031400. Mus musculus.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
ProDom	PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	285893.
SOURCE	Search...

Entry information

Entry name

G6PD1_MOUSE

Accession

Primary (citable) accession number: Q00612

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	March 20, 2007
Last modified:	November 25, 2008
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)