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Q00612

- G6PD1_MOUSE

UniProt

Q00612 - G6PD1_MOUSE

Protein

Glucose-6-phosphate 1-dehydrogenase X

Gene

G6pdx

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 3 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis By similarity.By similarity

    Catalytic activityi

    D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei72 – 721NADP 1By similarity
    Binding sitei147 – 1471NADP 1By similarity
    Binding sitei171 – 1711NADP 1; via carbonyl oxygenBy similarity
    Binding sitei171 – 1711SubstrateBy similarity
    Binding sitei239 – 2391SubstrateBy similarity
    Binding sitei258 – 2581SubstrateBy similarity
    Active sitei263 – 2631Proton acceptorBy similarity
    Binding sitei357 – 3571NADP 2By similarity
    Binding sitei360 – 3601SubstrateBy similarity
    Binding sitei365 – 3651SubstrateBy similarity
    Binding sitei366 – 3661NADP 2By similarity
    Binding sitei370 – 3701NADP 2By similarity
    Binding sitei393 – 3931NADP 2By similarity
    Binding sitei395 – 3951SubstrateBy similarity
    Binding sitei487 – 4871NADP 2By similarity
    Binding sitei503 – 5031NADP 2By similarity
    Binding sitei509 – 5091NADP 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi38 – 458NADP 1By similarity
    Nucleotide bindingi401 – 4033NADP 2By similarity
    Nucleotide bindingi421 – 4233NADP 2By similarity

    GO - Molecular functioni

    1. glucose-6-phosphate dehydrogenase activity Source: MGI
    2. NADP binding Source: InterPro
    3. protein homodimerization activity Source: MGI

    GO - Biological processi

    1. angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure Source: MGI
    2. cytokine production Source: MGI
    3. erythrocyte development Source: MGI
    4. glucose 6-phosphate metabolic process Source: UniProtKB
    5. glutathione metabolic process Source: MGI
    6. interleukin-10 production Source: MGI
    7. interleukin-12 production Source: MGI
    8. NADP biosynthetic process Source: MGI
    9. NADP metabolic process Source: UniProtKB
    10. pentose biosynthetic process Source: MGI
    11. pentose-phosphate shunt Source: UniProtKB-UniPathway
    12. regulation of multicellular organism growth Source: MGI
    13. response to oxidative stress Source: MGI
    14. vasodilation by angiotensin involved in regulation of systemic arterial blood pressure Source: MGI

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenase X (EC:1.1.1.49)
    Short name:
    G6PD
    Gene namesi
    Name:G6pdx
    Synonyms:G6pd, G6pd-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:105979. G6pdx.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 515514Glucose-6-phosphate 1-dehydrogenase XPRO_0000068085Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei403 – 4031N6-acetyllysineBy similarity
    Modified residuei507 – 5071Phosphotyrosine1 Publication

    Post-translational modificationi

    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ00612.
    PaxDbiQ00612.
    PRIDEiQ00612.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00228385.
    Q00612.

    PTM databases

    PhosphoSiteiQ00612.

    Expressioni

    Gene expression databases

    ArrayExpressiQ00612.
    BgeeiQ00612.
    CleanExiMM_G6PDX.
    GenevestigatoriQ00612.

    Interactioni

    Subunit structurei

    Homotetramer; dimer of dimers. Interacts with SIRT2; the interaction is enhanced by H2O2 treatment By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ00612. 3 interactions.
    MINTiMINT-4095477.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00612.
    SMRiQ00612. Positions 28-515.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni201 – 2055Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0364.
    HOGENOMiHOG000046192.
    HOVERGENiHBG000856.
    InParanoidiQ00612.
    KOiK00036.
    OMAiHAFTESS.
    OrthoDBiEOG7DRJ2T.
    PhylomeDBiQ00612.
    TreeFamiTF300584.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000110. G6PD. 1 hit.
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00612-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEQVALSRT QVCGILREEL YQGDAFHQAD THIFIIMGAS GDLAKKKIYP    50
    TIWWLFRDGL LPEDTFIVGY ARSRLTVDDI RKQSEPFFKA TPEERPKLEE 100
    FFARNSYVAG QYDDAASYKH LNSHMNALHQ GMQANRLFYL ALPPTVYEAV 150
    TKNIQETCMS QTGWNRIIVE KPFGRDLQSS NQLSNHISSL FREDQIYRID 200
    HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF 250
    DEFGIIRDVM QNHLLQMLCL VAMEKPATTG SDDVRDEKVK VLKCISEVET 300
    DNVVLGQYVG NPNGEGEAAN GYLDDPTVPH GSTTATFAAA VLYVENERWD 350
    GVPFILRCGK ALNERKAEVR LQFRDVAGDI FHQQCKRNEL VIRVQPNEAV 400
    YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM 450
    HFVRSDELRE AWRIFTPLLH KIDREKPQPI PYVYGSRGPT EADELMKRVG 500
    FQYEGTYKWV NPHKL 515
    Length:515
    Mass (Da):59,263
    Last modified:March 20, 2007 - v3
    Checksum:iC8AF0D4099B7AEC3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241D → Y in CAA37679. (PubMed:1874446)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11911 mRNA. Translation: CAA77967.1.
    AK088135 mRNA. Translation: BAC40166.1.
    X53617 Genomic DNA. Translation: CAA37679.1.
    U88533 Genomic DNA. Translation: AAB52998.1.
    U88534 Genomic DNA. Translation: AAB52999.1.
    CCDSiCCDS30232.1.
    PIRiA56686.
    RefSeqiNP_032088.1. NM_008062.2.
    UniGeneiMm.27210.

    Genome annotation databases

    EnsembliENSMUST00000004327; ENSMUSP00000004327; ENSMUSG00000031400.
    GeneIDi14381.
    KEGGimmu:14381.
    UCSCiuc009toy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11911 mRNA. Translation: CAA77967.1 .
    AK088135 mRNA. Translation: BAC40166.1 .
    X53617 Genomic DNA. Translation: CAA37679.1 .
    U88533 Genomic DNA. Translation: AAB52998.1 .
    U88534 Genomic DNA. Translation: AAB52999.1 .
    CCDSi CCDS30232.1.
    PIRi A56686.
    RefSeqi NP_032088.1. NM_008062.2.
    UniGenei Mm.27210.

    3D structure databases

    ProteinModelPortali Q00612.
    SMRi Q00612. Positions 28-515.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q00612. 3 interactions.
    MINTi MINT-4095477.

    PTM databases

    PhosphoSitei Q00612.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00228385.
    Q00612.

    Proteomic databases

    MaxQBi Q00612.
    PaxDbi Q00612.
    PRIDEi Q00612.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000004327 ; ENSMUSP00000004327 ; ENSMUSG00000031400 .
    GeneIDi 14381.
    KEGGi mmu:14381.
    UCSCi uc009toy.1. mouse.

    Organism-specific databases

    CTDi 14381.
    MGIi MGI:105979. G6pdx.

    Phylogenomic databases

    eggNOGi COG0364.
    HOGENOMi HOG000046192.
    HOVERGENi HBG000856.
    InParanoidi Q00612.
    KOi K00036.
    OMAi HAFTESS.
    OrthoDBi EOG7DRJ2T.
    PhylomeDBi Q00612.
    TreeFami TF300584.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00408 .

    Miscellaneous databases

    NextBioi 285893.
    PROi Q00612.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q00612.
    Bgeei Q00612.
    CleanExi MM_G6PDX.
    Genevestigatori Q00612.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00966. G6PD.
    InterProi IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000110. G6PD. 1 hit.
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR00871. zwf. 1 hit.
    PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of mouse glucose-6-phosphate dehydrogenase cDNA."
      Zollo M.Z., D'Urso M., Schlessinger D., Chen E.Y.
      DNA Seq. 3:319-322(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NOD.
      Tissue: Thymus.
    3. "The CpG island in the 5' region of the G6PD gene of man and mouse."
      Toniolo D., Filippi M., Dono R., Lettieri T., Martini G.
      Gene 102:197-203(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
      Strain: BALB/c.
    4. Lubec G., Klug S., Yang J.W., Zigmond M.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 57-71; 175-191 AND 247-257, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Brain and Hippocampus.
    5. "Somatic mutation of the glucose-6-phosphate dehydrogenase (g6pd) gene in colonic stem cells and crypt restricted loss of G6PD activity."
      Kuraguchi M., Thomas G.A., Williams E.D.
      Mutat. Res. 379:69-75(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 163-214 AND 352-454.
      Strain: BALB/c.
    6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-507, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.

    Entry informationi

    Entry nameiG6PD1_MOUSE
    AccessioniPrimary (citable) accession number: Q00612
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 135 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Has NADP both as cofactor (bound to the N-terminal domain) and as structural element bound to the C-terminal domain.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3