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Protein

T-lymphocyte activation antigen CD80

Gene

Cd80

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

GO - Biological processi

  • cellular response to lipopolysaccharide Source: MGI
  • positive regulation of alpha-beta T cell proliferation Source: MGI
  • positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • T cell costimulation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiR-MMU-1257604. PIP3 activates AKT signaling.
R-MMU-389356. CD28 co-stimulation.
R-MMU-389357. CD28 dependent PI3K/Akt signaling.
R-MMU-389359. CD28 dependent Vav1 pathway.
R-MMU-389513. CTLA4 inhibitory signaling.
R-MMU-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Protein family/group databases

MEROPSiI43.001.

Names & Taxonomyi

Protein namesi
Recommended name:
T-lymphocyte activation antigen CD80
Alternative name(s):
Activation B7-1 antigen
Short name:
B7
CD_antigen: CD80
Gene namesi
Name:Cd80
Synonyms:B7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:101775. Cd80.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 246ExtracellularSequence analysisAdd BLAST209
Transmembranei247 – 268HelicalSequence analysisAdd BLAST22
Topological domaini269 – 306CytoplasmicSequence analysisAdd BLAST38

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Add BLAST37
ChainiPRO_000001454838 – 306T-lymphocyte activation antigen CD80Add BLAST269

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 119PROSITE-ProRule annotation
Glycosylationi93N-linked (GlcNAc...)Sequence analysis1
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Glycosylationi149N-linked (GlcNAc...)1 Publication1
Glycosylationi155N-linked (GlcNAc...)1 Publication1
Disulfide bondi165 ↔ 219PROSITE-ProRule annotation
Glycosylationi189N-linked (GlcNAc...)1 Publication1
Glycosylationi210N-linked (GlcNAc...)1 Publication1
Glycosylationi214N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ00609.
PRIDEiQ00609.

PTM databases

iPTMnetiQ00609.
PhosphoSitePlusiQ00609.

Expressioni

Tissue specificityi

Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies.

Developmental stagei

Expressed between 4 and 12 hours post-activation. Protein was detected at cell surface at 24 hours and it's expression was maximal from 48 to 72 hours post-activation.

Gene expression databases

BgeeiENSMUSG00000075122.
CleanExiMM_CD80.
ExpressionAtlasiQ00609. baseline and differential.
GenevisibleiQ00609. MM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Cd274Q9EP734EBI-5258929,EBI-5258879

Protein-protein interaction databases

IntActiQ00609. 1 interactor.
STRINGi10090.ENSMUSP00000097404.

Structurei

Secondary structure

1306
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi40 – 45Combined sources6
Beta strandi50 – 52Combined sources3
Turni63 – 65Combined sources3
Beta strandi67 – 72Combined sources6
Beta strandi75 – 81Combined sources7
Beta strandi84 – 87Combined sources4
Helixi89 – 91Combined sources3
Turni92 – 94Combined sources3
Beta strandi95 – 98Combined sources4
Turni99 – 102Combined sources4
Beta strandi103 – 106Combined sources4
Helixi111 – 113Combined sources3
Beta strandi115 – 124Combined sources10
Beta strandi129 – 142Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RWHX-ray1.80A40-144[»]
ProteinModelPortaliQ00609.
SMRiQ00609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 135Ig-like V-typeAdd BLAST98
Domaini148 – 229Ig-like C2-typeAdd BLAST82

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni227 – 246Ig-hinge-likeSequence analysisAdd BLAST20

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J3XE. Eukaryota.
ENOG411154Z. LUCA.
GeneTreeiENSGT00720000108819.
HOGENOMiHOG000036959.
HOVERGENiHBG000261.
InParanoidiQ00609.
KOiK05412.
OMAiVRIYWQK.
OrthoDBiEOG091G0QRI.
PhylomeDBiQ00609.
TreeFamiTF351094.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q00609-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MACNCQLMQD TPLLKFPCPR LILLFVLLIR LSQVSSDVDE QLSKSVKDKV
60 70 80 90 100
LLPCRYNSPH EDESEDRIYW QKHDKVVLSV IAGKLKVWPE YKNRTLYDNT
110 120 130 140 150
TYSLIILGLV LSDRGTYSCV VQKKERGTYE VKHLALVKLS IKADFSTPNI
160 170 180 190 200
TESGNPSADT KRITCFASGG FPKPRFSWLE NGRELPGINT TISQDPESEL
210 220 230 240 250
YTISSQLDFN TTRNHTIKCL IKYGDAHVSE DFTWEKPPED PPDSKNTLVL
260 270 280 290 300
FGAGFGAVIT VVVIVVIIKC FCKHRSCFRR NEASRETNNS LTFGPEEALA

EQTVFL
Length:306
Mass (Da):34,590
Last modified:February 1, 1994 - v1
Checksum:i1DBADE0931B84C62
GO
Isoform 2 (identifier: Q00609-2) [UniParc]FASTAAdd to basket
Also known as: MB7-2

The sequence of this isoform differs from the canonical sequence as follows:
     144-237: Missing.

Show »
Length:212
Mass (Da):24,051
Checksum:iE51455E62260ED75
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012552144 – 237Missing in isoform 2. 1 PublicationAdd BLAST94

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60958 mRNA. Translation: CAA43291.1.
L12589
, L12585, L12586, L12587, L12588 Genomic DNA. Translation: AAA37240.1. Sequence problems.
D16220 mRNA. Translation: BAA03748.1.
CCDSiCCDS28168.1. [Q00609-1]
PIRiI49503.
RefSeqiNP_033985.3. NM_009855.2. [Q00609-1]
UniGeneiMm.89474.

Genome annotation databases

EnsembliENSMUST00000099816; ENSMUSP00000097404; ENSMUSG00000075122. [Q00609-1]
GeneIDi12519.
KEGGimmu:12519.
UCSCiuc007zez.2. mouse. [Q00609-1]
uc012afo.1. mouse. [Q00609-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60958 mRNA. Translation: CAA43291.1.
L12589
, L12585, L12586, L12587, L12588 Genomic DNA. Translation: AAA37240.1. Sequence problems.
D16220 mRNA. Translation: BAA03748.1.
CCDSiCCDS28168.1. [Q00609-1]
PIRiI49503.
RefSeqiNP_033985.3. NM_009855.2. [Q00609-1]
UniGeneiMm.89474.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RWHX-ray1.80A40-144[»]
ProteinModelPortaliQ00609.
SMRiQ00609.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ00609. 1 interactor.
STRINGi10090.ENSMUSP00000097404.

Protein family/group databases

MEROPSiI43.001.

PTM databases

iPTMnetiQ00609.
PhosphoSitePlusiQ00609.

Proteomic databases

PaxDbiQ00609.
PRIDEiQ00609.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099816; ENSMUSP00000097404; ENSMUSG00000075122. [Q00609-1]
GeneIDi12519.
KEGGimmu:12519.
UCSCiuc007zez.2. mouse. [Q00609-1]
uc012afo.1. mouse. [Q00609-2]

Organism-specific databases

CTDi941.
MGIiMGI:101775. Cd80.

Phylogenomic databases

eggNOGiENOG410J3XE. Eukaryota.
ENOG411154Z. LUCA.
GeneTreeiENSGT00720000108819.
HOGENOMiHOG000036959.
HOVERGENiHBG000261.
InParanoidiQ00609.
KOiK05412.
OMAiVRIYWQK.
OrthoDBiEOG091G0QRI.
PhylomeDBiQ00609.
TreeFamiTF351094.

Enzyme and pathway databases

ReactomeiR-MMU-1257604. PIP3 activates AKT signaling.
R-MMU-389356. CD28 co-stimulation.
R-MMU-389357. CD28 dependent PI3K/Akt signaling.
R-MMU-389359. CD28 dependent Vav1 pathway.
R-MMU-389513. CTLA4 inhibitory signaling.
R-MMU-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Miscellaneous databases

PROiQ00609.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000075122.
CleanExiMM_CD80.
ExpressionAtlasiQ00609. baseline and differential.
GenevisibleiQ00609. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD80_MOUSE
AccessioniPrimary (citable) accession number: Q00609
Secondary accession number(s): Q61332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.