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Q00609

- CD80_MOUSE

UniProt

Q00609 - CD80_MOUSE

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Protein

T-lymphocyte activation antigen CD80

Gene
Cd80, B7
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. positive regulation of alpha-beta T cell proliferation Source: MGI
  2. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  3. positive regulation of T cell proliferation Source: MGI
  4. T cell costimulation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiREACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_204081. CD28 co-stimulation.
REACT_226151. CD28 dependent PI3K/Akt signaling.
REACT_226341. PIP3 activates AKT signaling.

Protein family/group databases

MEROPSiI43.001.

Names & Taxonomyi

Protein namesi
Recommended name:
T-lymphocyte activation antigen CD80
Alternative name(s):
Activation B7-1 antigen
Short name:
B7
CD_antigen: CD80
Gene namesi
Name:Cd80
Synonyms:B7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:101775. Cd80.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini38 – 246209Extracellular Reviewed predictionAdd
BLAST
Transmembranei247 – 26822Helical; Reviewed predictionAdd
BLAST
Topological domaini269 – 30638Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. external side of plasma membrane Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737Add
BLAST
Chaini38 – 306269T-lymphocyte activation antigen CD80PRO_0000014548Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 119 Reviewed prediction
Glycosylationi93 – 931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi99 – 991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi149 – 1491N-linked (GlcNAc...)1 Publication
Glycosylationi155 – 1551N-linked (GlcNAc...)1 Publication
Disulfide bondi165 ↔ 219 Reviewed prediction
Glycosylationi189 – 1891N-linked (GlcNAc...)1 Publication
Glycosylationi210 – 2101N-linked (GlcNAc...)1 Publication
Glycosylationi214 – 2141N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ00609.
PRIDEiQ00609.

PTM databases

PhosphoSiteiQ00609.

Expressioni

Tissue specificityi

Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies.

Developmental stagei

Expressed between 4 and 12 hours post-activation. Protein was detected at cell surface at 24 hours and it's expression was maximal from 48 to 72 hours post-activation.

Gene expression databases

ArrayExpressiQ00609.
BgeeiQ00609.
CleanExiMM_CD80.
GenevestigatoriQ00609.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Cd274Q9EP734EBI-5258929,EBI-5258879

Protein-protein interaction databases

IntActiQ00609. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ00609.
SMRiQ00609. Positions 41-234.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 13598Ig-like V-typeAdd
BLAST
Domaini148 – 22982Ig-like C2-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni227 – 24620Ig-hinge-like Reviewed predictionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG319383.
HOGENOMiHOG000036959.
HOVERGENiHBG000261.
InParanoidiQ00609.
KOiK05412.
OMAiVRIYWQK.
OrthoDBiEOG7VQJFD.
PhylomeDBiQ00609.
TreeFamiTF351094.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q00609-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MACNCQLMQD TPLLKFPCPR LILLFVLLIR LSQVSSDVDE QLSKSVKDKV    50
LLPCRYNSPH EDESEDRIYW QKHDKVVLSV IAGKLKVWPE YKNRTLYDNT 100
TYSLIILGLV LSDRGTYSCV VQKKERGTYE VKHLALVKLS IKADFSTPNI 150
TESGNPSADT KRITCFASGG FPKPRFSWLE NGRELPGINT TISQDPESEL 200
YTISSQLDFN TTRNHTIKCL IKYGDAHVSE DFTWEKPPED PPDSKNTLVL 250
FGAGFGAVIT VVVIVVIIKC FCKHRSCFRR NEASRETNNS LTFGPEEALA 300
EQTVFL 306
Length:306
Mass (Da):34,590
Last modified:February 1, 1994 - v1
Checksum:i1DBADE0931B84C62
GO
Isoform 2 (identifier: Q00609-2) [UniParc]FASTAAdd to Basket

Also known as: MB7-2

The sequence of this isoform differs from the canonical sequence as follows:
     144-237: Missing.

Show »
Length:212
Mass (Da):24,051
Checksum:iE51455E62260ED75
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei144 – 23794Missing in isoform 2. VSP_012552Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60958 mRNA. Translation: CAA43291.1.
L12589
, L12585, L12586, L12587, L12588 Genomic DNA. Translation: AAA37240.1. Sequence problems.
D16220 mRNA. Translation: BAA03748.1.
CCDSiCCDS28168.1. [Q00609-1]
PIRiI49503.
RefSeqiNP_033985.3. NM_009855.2. [Q00609-1]
UniGeneiMm.89474.

Genome annotation databases

EnsembliENSMUST00000099816; ENSMUSP00000097404; ENSMUSG00000075122. [Q00609-1]
GeneIDi12519.
KEGGimmu:12519.
UCSCiuc007zez.1. mouse. [Q00609-1]
uc012afo.1. mouse. [Q00609-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60958 mRNA. Translation: CAA43291.1 .
L12589
, L12585 , L12586 , L12587 , L12588 Genomic DNA. Translation: AAA37240.1 . Sequence problems.
D16220 mRNA. Translation: BAA03748.1 .
CCDSi CCDS28168.1. [Q00609-1 ]
PIRi I49503.
RefSeqi NP_033985.3. NM_009855.2. [Q00609-1 ]
UniGenei Mm.89474.

3D structure databases

ProteinModelPortali Q00609.
SMRi Q00609. Positions 41-234.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q00609. 1 interaction.

Protein family/group databases

MEROPSi I43.001.

PTM databases

PhosphoSitei Q00609.

Proteomic databases

PaxDbi Q00609.
PRIDEi Q00609.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000099816 ; ENSMUSP00000097404 ; ENSMUSG00000075122 . [Q00609-1 ]
GeneIDi 12519.
KEGGi mmu:12519.
UCSCi uc007zez.1. mouse. [Q00609-1 ]
uc012afo.1. mouse. [Q00609-2 ]

Organism-specific databases

CTDi 941.
MGIi MGI:101775. Cd80.

Phylogenomic databases

eggNOGi NOG319383.
HOGENOMi HOG000036959.
HOVERGENi HBG000261.
InParanoidi Q00609.
KOi K05412.
OMAi VRIYWQK.
OrthoDBi EOG7VQJFD.
PhylomeDBi Q00609.
TreeFami TF351094.

Enzyme and pathway databases

Reactomei REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_204081. CD28 co-stimulation.
REACT_226151. CD28 dependent PI3K/Akt signaling.
REACT_226341. PIP3 activates AKT signaling.

Miscellaneous databases

NextBioi 281522.
PROi Q00609.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q00609.
Bgeei Q00609.
CleanExi MM_CD80.
Genevestigatori Q00609.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
InterProi IPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view ]
Pfami PF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view ]
SMARTi SM00409. IG. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7."
    Freeman G.J., Gray G.S., Gimmi C.D., Lombard D.B., Zhou L.-J., White M., Fingeroth J.D., Gribben J.G., Nadler L.M.
    J. Exp. Med. 174:625-631(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: B-cell.
  2. "Genomic organization of the mouse B-lymphocyte activation antigen B7."
    Selvakumar A., White P.C., Dupont B.
    Immunogenetics 38:292-295(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: B-cell.
  3. "Identification of an alternatively spliced form of the murine homologue of B7."
    Inobe M., Linsley P.S., Ledbetter J.A., Nagai Y., Tamakoshi M., Uede T.
    Biochem. Biophys. Res. Commun. 200:443-449(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Spleen.
  4. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-149; ASN-155; ASN-189; ASN-210 AND ASN-214.
    Tissue: Myoblast.

Entry informationi

Entry nameiCD80_MOUSE
AccessioniPrimary (citable) accession number: Q00609
Secondary accession number(s): Q61332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi