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Q00609 (CD80_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-lymphocyte activation antigen CD80
Alternative name(s):
Activation B7-1 antigen
Short name=B7
CD_antigen=CD80
Gene names
Name:Cd80
Synonyms:B7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies.

Developmental stage

Expressed between 4 and 12 hours post-activation. Protein was detected at cell surface at 24 hours and it's expression was maximal from 48 to 72 hours post-activation.

Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Cd274Q9EP734EBI-5258929,EBI-5258879

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q00609-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q00609-2)

Also known as: MB7-2;

The sequence of this isoform differs from the canonical sequence as follows:
     144-237: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3737
Chain38 – 306269T-lymphocyte activation antigen CD80
PRO_0000014548

Regions

Topological domain38 – 246209Extracellular Potential
Transmembrane247 – 26822Helical; Potential
Topological domain269 – 30638Cytoplasmic Potential
Domain38 – 13598Ig-like V-type
Domain148 – 22982Ig-like C2-type
Region227 – 24620Ig-hinge-like Potential

Amino acid modifications

Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation1491N-linked (GlcNAc...) Ref.4
Glycosylation1551N-linked (GlcNAc...) Ref.4
Glycosylation1891N-linked (GlcNAc...) Ref.4
Glycosylation2101N-linked (GlcNAc...) Ref.4
Glycosylation2141N-linked (GlcNAc...) Ref.4
Disulfide bond54 ↔ 119 Potential
Disulfide bond165 ↔ 219 Potential

Natural variations

Alternative sequence144 – 23794Missing in isoform 2.
VSP_012552

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 1DBADE0931B84C62

FASTA30634,590
        10         20         30         40         50         60 
MACNCQLMQD TPLLKFPCPR LILLFVLLIR LSQVSSDVDE QLSKSVKDKV LLPCRYNSPH 

        70         80         90        100        110        120 
EDESEDRIYW QKHDKVVLSV IAGKLKVWPE YKNRTLYDNT TYSLIILGLV LSDRGTYSCV 

       130        140        150        160        170        180 
VQKKERGTYE VKHLALVKLS IKADFSTPNI TESGNPSADT KRITCFASGG FPKPRFSWLE 

       190        200        210        220        230        240 
NGRELPGINT TISQDPESEL YTISSQLDFN TTRNHTIKCL IKYGDAHVSE DFTWEKPPED 

       250        260        270        280        290        300 
PPDSKNTLVL FGAGFGAVIT VVVIVVIIKC FCKHRSCFRR NEASRETNNS LTFGPEEALA 


EQTVFL 

« Hide

Isoform 2 (MB7-2) [UniParc].

Checksum: E51455E62260ED75
Show »

FASTA21224,051

References

« Hide 'large scale' references
[1]"Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7."
Freeman G.J., Gray G.S., Gimmi C.D., Lombard D.B., Zhou L.-J., White M., Fingeroth J.D., Gribben J.G., Nadler L.M.
J. Exp. Med. 174:625-631(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: B-cell.
[2]"Genomic organization of the mouse B-lymphocyte activation antigen B7."
Selvakumar A., White P.C., Dupont B.
Immunogenetics 38:292-295(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: B-cell.
[3]"Identification of an alternatively spliced form of the murine homologue of B7."
Inobe M., Linsley P.S., Ledbetter J.A., Nagai Y., Tamakoshi M., Uede T.
Biochem. Biophys. Res. Commun. 200:443-449(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Spleen.
[4]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-149; ASN-155; ASN-189; ASN-210 AND ASN-214.
Tissue: Myoblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60958 mRNA. Translation: CAA43291.1.
L12589 expand/collapse EMBL AC list , L12585, L12586, L12587, L12588 Genomic DNA. Translation: AAA37240.1. Sequence problems.
D16220 mRNA. Translation: BAA03748.1.
PIRI49503.
RefSeqNP_033985.3. NM_009855.2.
UniGeneMm.89474.

3D structure databases

ProteinModelPortalQ00609.
SMRQ00609. Positions 41-234.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ00609. 1 interaction.

Protein family/group databases

MEROPSI43.001.

PTM databases

PhosphoSiteQ00609.

Proteomic databases

PaxDbQ00609.
PRIDEQ00609.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000099816; ENSMUSP00000097404; ENSMUSG00000075122. [Q00609-1]
GeneID12519.
KEGGmmu:12519.
UCSCuc007zez.1. mouse. [Q00609-1]
uc012afo.1. mouse. [Q00609-2]

Organism-specific databases

CTD941.
MGIMGI:101775. Cd80.

Phylogenomic databases

eggNOGNOG319383.
HOGENOMHOG000036959.
HOVERGENHBG000261.
InParanoidQ00609.
KOK05412.
OMAVRIYWQK.
OrthoDBEOG7VQJFD.
PhylomeDBQ00609.
TreeFamTF351094.

Gene expression databases

ArrayExpressQ00609.
BgeeQ00609.
CleanExMM_CD80.
GenevestigatorQ00609.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio281522.
PROQ00609.
SOURCESearch...

Entry information

Entry nameCD80_MOUSE
AccessionPrimary (citable) accession number: Q00609
Secondary accession number(s): Q61332
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot