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Protein

T-lymphocyte activation antigen CD80

Gene

Cd80

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

GO - Biological processi

  1. positive regulation of alpha-beta T cell proliferation Source: MGI
  2. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  3. positive regulation of T cell proliferation Source: MGI
  4. T cell costimulation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiREACT_311393. CTLA4 inhibitory signaling.
REACT_314817. CD28 dependent Vav1 pathway.
REACT_327235. CD28 dependent PI3K/Akt signaling.
REACT_328674. Constitutive PI3K/AKT Signaling in Cancer.
REACT_333474. PIP3 activates AKT signaling.
REACT_334921. CD28 co-stimulation.

Protein family/group databases

MEROPSiI43.001.

Names & Taxonomyi

Protein namesi
Recommended name:
T-lymphocyte activation antigen CD80
Alternative name(s):
Activation B7-1 antigen
Short name:
B7
CD_antigen: CD80
Gene namesi
Name:Cd80
Synonyms:B7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:101775. Cd80.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini38 – 246209ExtracellularSequence AnalysisAdd
BLAST
Transmembranei247 – 26822HelicalSequence AnalysisAdd
BLAST
Topological domaini269 – 30638CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. external side of plasma membrane Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737Add
BLAST
Chaini38 – 306269T-lymphocyte activation antigen CD80PRO_0000014548Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 119PROSITE-ProRule annotation
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi149 – 1491N-linked (GlcNAc...)1 Publication
Glycosylationi155 – 1551N-linked (GlcNAc...)1 Publication
Disulfide bondi165 ↔ 219PROSITE-ProRule annotation
Glycosylationi189 – 1891N-linked (GlcNAc...)1 Publication
Glycosylationi210 – 2101N-linked (GlcNAc...)1 Publication
Glycosylationi214 – 2141N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ00609.
PRIDEiQ00609.

PTM databases

PhosphoSiteiQ00609.

Expressioni

Tissue specificityi

Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies.

Developmental stagei

Expressed between 4 and 12 hours post-activation. Protein was detected at cell surface at 24 hours and it's expression was maximal from 48 to 72 hours post-activation.

Gene expression databases

BgeeiQ00609.
CleanExiMM_CD80.
ExpressionAtlasiQ00609. baseline and differential.
GenevestigatoriQ00609.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Cd274Q9EP734EBI-5258929,EBI-5258879

Protein-protein interaction databases

IntActiQ00609. 1 interaction.

Structurei

Secondary structure

1
306
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi40 – 456Combined sources
Beta strandi50 – 523Combined sources
Turni63 – 653Combined sources
Beta strandi67 – 726Combined sources
Beta strandi75 – 817Combined sources
Beta strandi84 – 874Combined sources
Helixi89 – 913Combined sources
Turni92 – 943Combined sources
Beta strandi95 – 984Combined sources
Turni99 – 1024Combined sources
Beta strandi103 – 1064Combined sources
Helixi111 – 1133Combined sources
Beta strandi115 – 12410Combined sources
Beta strandi129 – 14214Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RWHX-ray1.80A40-144[»]
ProteinModelPortaliQ00609.
SMRiQ00609. Positions 41-234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 13598Ig-like V-typeAdd
BLAST
Domaini148 – 22982Ig-like C2-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni227 – 24620Ig-hinge-likeSequence AnalysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG319383.
HOGENOMiHOG000036959.
HOVERGENiHBG000261.
InParanoidiQ00609.
KOiK05412.
OMAiVRIYWQK.
OrthoDBiEOG7VQJFD.
PhylomeDBiQ00609.
TreeFamiTF351094.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q00609-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MACNCQLMQD TPLLKFPCPR LILLFVLLIR LSQVSSDVDE QLSKSVKDKV
60 70 80 90 100
LLPCRYNSPH EDESEDRIYW QKHDKVVLSV IAGKLKVWPE YKNRTLYDNT
110 120 130 140 150
TYSLIILGLV LSDRGTYSCV VQKKERGTYE VKHLALVKLS IKADFSTPNI
160 170 180 190 200
TESGNPSADT KRITCFASGG FPKPRFSWLE NGRELPGINT TISQDPESEL
210 220 230 240 250
YTISSQLDFN TTRNHTIKCL IKYGDAHVSE DFTWEKPPED PPDSKNTLVL
260 270 280 290 300
FGAGFGAVIT VVVIVVIIKC FCKHRSCFRR NEASRETNNS LTFGPEEALA

EQTVFL
Length:306
Mass (Da):34,590
Last modified:February 1, 1994 - v1
Checksum:i1DBADE0931B84C62
GO
Isoform 2 (identifier: Q00609-2) [UniParc]FASTAAdd to basket

Also known as: MB7-2

The sequence of this isoform differs from the canonical sequence as follows:
     144-237: Missing.

Show »
Length:212
Mass (Da):24,051
Checksum:iE51455E62260ED75
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei144 – 23794Missing in isoform 2. 1 PublicationVSP_012552Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60958 mRNA. Translation: CAA43291.1.
L12589
, L12585, L12586, L12587, L12588 Genomic DNA. Translation: AAA37240.1. Sequence problems.
D16220 mRNA. Translation: BAA03748.1.
CCDSiCCDS28168.1. [Q00609-1]
PIRiI49503.
RefSeqiNP_033985.3. NM_009855.2. [Q00609-1]
UniGeneiMm.89474.

Genome annotation databases

EnsembliENSMUST00000099816; ENSMUSP00000097404; ENSMUSG00000075122. [Q00609-1]
GeneIDi12519.
KEGGimmu:12519.
UCSCiuc007zez.1. mouse. [Q00609-1]
uc012afo.1. mouse. [Q00609-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60958 mRNA. Translation: CAA43291.1.
L12589
, L12585, L12586, L12587, L12588 Genomic DNA. Translation: AAA37240.1. Sequence problems.
D16220 mRNA. Translation: BAA03748.1.
CCDSiCCDS28168.1. [Q00609-1]
PIRiI49503.
RefSeqiNP_033985.3. NM_009855.2. [Q00609-1]
UniGeneiMm.89474.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RWHX-ray1.80A40-144[»]
ProteinModelPortaliQ00609.
SMRiQ00609. Positions 41-234.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ00609. 1 interaction.

Protein family/group databases

MEROPSiI43.001.

PTM databases

PhosphoSiteiQ00609.

Proteomic databases

PaxDbiQ00609.
PRIDEiQ00609.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099816; ENSMUSP00000097404; ENSMUSG00000075122. [Q00609-1]
GeneIDi12519.
KEGGimmu:12519.
UCSCiuc007zez.1. mouse. [Q00609-1]
uc012afo.1. mouse. [Q00609-2]

Organism-specific databases

CTDi941.
MGIiMGI:101775. Cd80.

Phylogenomic databases

eggNOGiNOG319383.
HOGENOMiHOG000036959.
HOVERGENiHBG000261.
InParanoidiQ00609.
KOiK05412.
OMAiVRIYWQK.
OrthoDBiEOG7VQJFD.
PhylomeDBiQ00609.
TreeFamiTF351094.

Enzyme and pathway databases

ReactomeiREACT_311393. CTLA4 inhibitory signaling.
REACT_314817. CD28 dependent Vav1 pathway.
REACT_327235. CD28 dependent PI3K/Akt signaling.
REACT_328674. Constitutive PI3K/AKT Signaling in Cancer.
REACT_333474. PIP3 activates AKT signaling.
REACT_334921. CD28 co-stimulation.

Miscellaneous databases

NextBioi281522.
PROiQ00609.
SOURCEiSearch...

Gene expression databases

BgeeiQ00609.
CleanExiMM_CD80.
ExpressionAtlasiQ00609. baseline and differential.
GenevestigatoriQ00609.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7."
    Freeman G.J., Gray G.S., Gimmi C.D., Lombard D.B., Zhou L.-J., White M., Fingeroth J.D., Gribben J.G., Nadler L.M.
    J. Exp. Med. 174:625-631(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: B-cell.
  2. "Genomic organization of the mouse B-lymphocyte activation antigen B7."
    Selvakumar A., White P.C., Dupont B.
    Immunogenetics 38:292-295(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: B-cell.
  3. "Identification of an alternatively spliced form of the murine homologue of B7."
    Inobe M., Linsley P.S., Ledbetter J.A., Nagai Y., Tamakoshi M., Uede T.
    Biochem. Biophys. Res. Commun. 200:443-449(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Spleen.
  4. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-149; ASN-155; ASN-189; ASN-210 AND ASN-214.
    Tissue: Myoblast.

Entry informationi

Entry nameiCD80_MOUSE
AccessioniPrimary (citable) accession number: Q00609
Secondary accession number(s): Q61332
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 1, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.