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Q00609

- CD80_MOUSE

UniProt

Q00609 - CD80_MOUSE

Protein

T-lymphocyte activation antigen CD80

Gene

Cd80

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. positive regulation of alpha-beta T cell proliferation Source: MGI
    2. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
    3. positive regulation of T cell proliferation Source: MGI
    4. T cell costimulation Source: MGI

    Keywords - Molecular functioni

    Receptor

    Enzyme and pathway databases

    ReactomeiREACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_204081. CD28 co-stimulation.
    REACT_226151. CD28 dependent PI3K/Akt signaling.
    REACT_226341. PIP3 activates AKT signaling.

    Protein family/group databases

    MEROPSiI43.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-lymphocyte activation antigen CD80
    Alternative name(s):
    Activation B7-1 antigen
    Short name:
    B7
    CD_antigen: CD80
    Gene namesi
    Name:Cd80
    Synonyms:B7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:101775. Cd80.

    Subcellular locationi

    GO - Cellular componenti

    1. external side of plasma membrane Source: MGI
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3737Add
    BLAST
    Chaini38 – 306269T-lymphocyte activation antigen CD80PRO_0000014548Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi54 ↔ 119PROSITE-ProRule annotation
    Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi149 – 1491N-linked (GlcNAc...)1 Publication
    Glycosylationi155 – 1551N-linked (GlcNAc...)1 Publication
    Disulfide bondi165 ↔ 219PROSITE-ProRule annotation
    Glycosylationi189 – 1891N-linked (GlcNAc...)1 Publication
    Glycosylationi210 – 2101N-linked (GlcNAc...)1 Publication
    Glycosylationi214 – 2141N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ00609.
    PRIDEiQ00609.

    PTM databases

    PhosphoSiteiQ00609.

    Expressioni

    Tissue specificityi

    Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies.

    Developmental stagei

    Expressed between 4 and 12 hours post-activation. Protein was detected at cell surface at 24 hours and it's expression was maximal from 48 to 72 hours post-activation.

    Gene expression databases

    ArrayExpressiQ00609.
    BgeeiQ00609.
    CleanExiMM_CD80.
    GenevestigatoriQ00609.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cd274Q9EP734EBI-5258929,EBI-5258879

    Protein-protein interaction databases

    IntActiQ00609. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00609.
    SMRiQ00609. Positions 41-234.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini38 – 246209ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini269 – 30638CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei247 – 26822HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 13598Ig-like V-typeAdd
    BLAST
    Domaini148 – 22982Ig-like C2-typeAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni227 – 24620Ig-hinge-likeSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG319383.
    HOGENOMiHOG000036959.
    HOVERGENiHBG000261.
    InParanoidiQ00609.
    KOiK05412.
    OMAiVRIYWQK.
    OrthoDBiEOG7VQJFD.
    PhylomeDBiQ00609.
    TreeFamiTF351094.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    InterProiIPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view]
    PfamiPF08205. C2-set_2. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q00609-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MACNCQLMQD TPLLKFPCPR LILLFVLLIR LSQVSSDVDE QLSKSVKDKV    50
    LLPCRYNSPH EDESEDRIYW QKHDKVVLSV IAGKLKVWPE YKNRTLYDNT 100
    TYSLIILGLV LSDRGTYSCV VQKKERGTYE VKHLALVKLS IKADFSTPNI 150
    TESGNPSADT KRITCFASGG FPKPRFSWLE NGRELPGINT TISQDPESEL 200
    YTISSQLDFN TTRNHTIKCL IKYGDAHVSE DFTWEKPPED PPDSKNTLVL 250
    FGAGFGAVIT VVVIVVIIKC FCKHRSCFRR NEASRETNNS LTFGPEEALA 300
    EQTVFL 306
    Length:306
    Mass (Da):34,590
    Last modified:February 1, 1994 - v1
    Checksum:i1DBADE0931B84C62
    GO
    Isoform 2 (identifier: Q00609-2) [UniParc]FASTAAdd to Basket

    Also known as: MB7-2

    The sequence of this isoform differs from the canonical sequence as follows:
         144-237: Missing.

    Show »
    Length:212
    Mass (Da):24,051
    Checksum:iE51455E62260ED75
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei144 – 23794Missing in isoform 2. 1 PublicationVSP_012552Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60958 mRNA. Translation: CAA43291.1.
    L12589
    , L12585, L12586, L12587, L12588 Genomic DNA. Translation: AAA37240.1. Sequence problems.
    D16220 mRNA. Translation: BAA03748.1.
    CCDSiCCDS28168.1. [Q00609-1]
    PIRiI49503.
    RefSeqiNP_033985.3. NM_009855.2. [Q00609-1]
    UniGeneiMm.89474.

    Genome annotation databases

    EnsembliENSMUST00000099816; ENSMUSP00000097404; ENSMUSG00000075122. [Q00609-1]
    GeneIDi12519.
    KEGGimmu:12519.
    UCSCiuc007zez.1. mouse. [Q00609-1]
    uc012afo.1. mouse. [Q00609-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60958 mRNA. Translation: CAA43291.1 .
    L12589
    , L12585 , L12586 , L12587 , L12588 Genomic DNA. Translation: AAA37240.1 . Sequence problems.
    D16220 mRNA. Translation: BAA03748.1 .
    CCDSi CCDS28168.1. [Q00609-1 ]
    PIRi I49503.
    RefSeqi NP_033985.3. NM_009855.2. [Q00609-1 ]
    UniGenei Mm.89474.

    3D structure databases

    ProteinModelPortali Q00609.
    SMRi Q00609. Positions 41-234.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q00609. 1 interaction.

    Protein family/group databases

    MEROPSi I43.001.

    PTM databases

    PhosphoSitei Q00609.

    Proteomic databases

    PaxDbi Q00609.
    PRIDEi Q00609.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000099816 ; ENSMUSP00000097404 ; ENSMUSG00000075122 . [Q00609-1 ]
    GeneIDi 12519.
    KEGGi mmu:12519.
    UCSCi uc007zez.1. mouse. [Q00609-1 ]
    uc012afo.1. mouse. [Q00609-2 ]

    Organism-specific databases

    CTDi 941.
    MGIi MGI:101775. Cd80.

    Phylogenomic databases

    eggNOGi NOG319383.
    HOGENOMi HOG000036959.
    HOVERGENi HBG000261.
    InParanoidi Q00609.
    KOi K05412.
    OMAi VRIYWQK.
    OrthoDBi EOG7VQJFD.
    PhylomeDBi Q00609.
    TreeFami TF351094.

    Enzyme and pathway databases

    Reactomei REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_204081. CD28 co-stimulation.
    REACT_226151. CD28 dependent PI3K/Akt signaling.
    REACT_226341. PIP3 activates AKT signaling.

    Miscellaneous databases

    NextBioi 281522.
    PROi Q00609.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q00609.
    Bgeei Q00609.
    CleanExi MM_CD80.
    Genevestigatori Q00609.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    InterProi IPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view ]
    Pfami PF08205. C2-set_2. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7."
      Freeman G.J., Gray G.S., Gimmi C.D., Lombard D.B., Zhou L.-J., White M., Fingeroth J.D., Gribben J.G., Nadler L.M.
      J. Exp. Med. 174:625-631(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: B-cell.
    2. "Genomic organization of the mouse B-lymphocyte activation antigen B7."
      Selvakumar A., White P.C., Dupont B.
      Immunogenetics 38:292-295(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: B-cell.
    3. "Identification of an alternatively spliced form of the murine homologue of B7."
      Inobe M., Linsley P.S., Ledbetter J.A., Nagai Y., Tamakoshi M., Uede T.
      Biochem. Biophys. Res. Commun. 200:443-449(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: C57BL/6.
      Tissue: Spleen.
    4. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
      Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
      Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-149; ASN-155; ASN-189; ASN-210 AND ASN-214.
      Tissue: Myoblast.

    Entry informationi

    Entry nameiCD80_MOUSE
    AccessioniPrimary (citable) accession number: Q00609
    Secondary accession number(s): Q61332
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3