Q00598 (FENR_CYAPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferredoxin--NADP reductase, cyanelle
Short name=FNR
EC=1.18.1.2

Gene names

Name:

PETH

Organism

Cyanophora paradoxa

Taxonomic identifier

2762 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Glaucocystophyceae › Cyanophoraceae › Cyanophora

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.
Catalytic activity	2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.
Cofactor	FAD.
Subcellular location	Plastid › cyanelle stroma. Plastid › cyanelle thylakoid membrane; Peripheral membrane protein; Stromal side Probable. Note: In the vicinity of the photosystem I By similarity.
Sequence similarities	Belongs to the ferredoxin--NADP reductase type 1 family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Cyanelle Membrane Plastid Thylakoid
Domain	Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cyanelle stroma Inferred from electronic annotation. Source: UniProtKB-SubCell cyanelle thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro ferredoxin-NADP+ reductase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 65	65	Cyanelle Ref.1
	Chain	66 – 363	298	Ferredoxin--NADP reductase, cyanelle		PRO_0000019418
Regions
	Domain	84 – 206	123	FAD-binding FR-type
	Nucleotide binding	142 – 145	4	FAD By similarity
	Nucleotide binding	163 – 165	3	FAD By similarity
	Nucleotide binding	180 – 182	3	FAD By similarity
	Nucleotide binding	253 – 254	2	NADP By similarity
	Nucleotide binding	283 – 284	2	NADP By similarity
	Nucleotide binding	322 – 323	2	NADP By similarity
Sites
	Binding site	145	1	NADP By similarity
	Binding site	165	1	NADP By similarity
	Binding site	169	1	FAD By similarity
	Binding site	221	1	FAD By similarity
	Binding site	221	1	NADP; via amide nitrogen By similarity
	Binding site	293	1	NADP By similarity
	Binding site	361	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00598 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: F5F5D1AEA91ADEBF	FASTA	363	40,491
	10 20 30 40 50 60 MAFVASVPVF ANASGLKTEA KVCQKPALKN SFFRGEEVTS RSFFASQAVS AKPATTFEVD 70 80 90 100 110 120 TTIRAQAVDA KKKGDIPLNL FRPANPYIGK CIYNERIVGE GAPGETKHII FTHEGKVPYL 130 140 150 160 170 180 EGQSIGIIPP GTDKDGKPHK LRLYSIASTR HGDFGDDKTV SLSVKRLEYT DANGNLVKGV 190 200 210 220 230 240 CSNYLCDLKP GDEVMITGPV GTTMLMPEDQ SATIIMLATG TGIAPFRSFL RRMFEETHAD 250 260 270 280 290 300 YKFNGLAWLF LGVPTSSTLL YREELEKMQK ANPNNFRLDY AISREQTDSK GEKMYIQNRI 310 320 330 340 350 360 AEYANEFWNM IQKPNTFVYM CGLRGMEDGI QQCMEDIAKA NGTTWDAVVK GLKKEKRWHV ETY

References

[1]

"Sequence analysis of pre-ferredoxin-NADP(+)-reductase cDNA from Cyanophora paradoxa specifying a precursor for a nucleus-encoded cyanelle polypeptide." Jakowitsch J., Bayer M.G., Maier T.L., Luettke A., Gebhart U.B., Brandtner M., Hamilton B., Neumann-Spallart C., Michalowski C.B., Bohnert H.J., Schenk H.E.A., Loeffelhardt W. Plant Mol. Biol. 21:1023-1033(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 66-90. Strain: UTEX LB 555 / Pringsheim.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66372 mRNA. Translation: CAA47015.1.
PIR	A56664. S33545.
3D structure databases
ProteinModelPortal	Q00598.
SMR	Q00598. Positions 62-363.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR015701. Fd_Red. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR012146. FNR. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
PANTHER	PTHR19384:SF1. PTHR19384:SF1. 1 hit.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF000361. Frd-NADP+_RD. 1 hit.
PRINTS	PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FENR_CYAPA

Accession

Primary (citable) accession number: Q00598

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)