Reviewed, UniProtKB/Swiss-Prot Q00593 (ALKJ_PSEOL)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase [acceptor]
    EC=1.1.99.-

Gene names

Name:	alkJ
Synonyms:	alcO

Encoded on

Plasmid OCT

Organism

Pseudomonas oleovorans

Taxonomic identifier

301 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain.
Catalytic activity	Alcohol + acceptor = aldehyde + reduced acceptor.
Cofactor	FAD By similarity.
Subcellular location	Cell inner membrane.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on CH-OH group of donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Alcohol dehydrogenase [acceptor]		PRO_0000205578
Regions
	Nucleotide binding	3 – 32	30	FAD Potential
Sites
	Active site	469	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00593-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: DF4363ECC71B02E8	FASTA	558	61,195
	10 20 30 40 50 60 MYDYIIVGAG SAGCVLANRL SADPSKRVCL LEAGPRDTNP LIHMPLGIAL LSNSKKLNWA 70 80 90 100 110 120 FQTAPQQNLN GRSLFWPRGK TLGGSSSINA MVYIRGHEDD YHAWEQAAGR YWGWYRALEL 130 140 150 160 170 180 FKRLECNQRF DKSEHHGVDG ELAVSDLKYI NPLSKAFVQA GMEANINFNG DFNGEYQDGV 190 200 210 220 230 240 GFYQVTQKNG QRWSSARAFL HGVLSRPNLD IITDAHASKI LFEDRKAVGV SYIKKNMHHQ 250 260 270 280 290 300 VKTTSGGEVL LSLGAVGTPH LLMLSGVGAA AELKEHGVSL VHDLPEVGKN LQDHLDITLM 310 320 330 340 350 360 CAANSREPIG VALSFIPRGV SGLFSYVFKR EGFLTSNVAE SGGFVKSSPD RDRPNLQFHF 370 380 390 400 410 420 LPTYLKDHGR KIAGGYGYTL HICDLLPKSR GRIGLKSANP LQPPLIDPNY LSDHEDIKTM 430 440 450 460 470 480 IAGIKIGRAI LQAPSMAKHF KHEVVPGQAV KTDDEIIEDI RRRAETIYHP VGTCRMGKDP 490 500 510 520 530 540 ASVVDPCLKI RGLANIRVVD ASIMPHLVAG NTNAPTIMIA ENAAEIIMRN LDVEALEASA 550 EFAREGAELE LAMIAVCM

References

[1]

"DNA sequence determination and functional characterization of the OCT-plasmid-encoded alkJKL genes of Pseudomonas oleovorans." van Beilen J.B., Eggink G., Enequist H., Bos R., Witholt B. Mol. Microbiol. 6:3121-3136(1992) [PubMed: 1453953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10. Strain: GPo1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ245436 Genomic DNA. Translation: CAB54054.1.
PIR	S27994.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-1081.
Family and domain databases
InterPro	IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALKJ_PSEOL

Accession

Primary (citable) accession number: Q00593

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)