Reviewed, UniProtKB/Swiss-Prot Q00583 (HMDH3_HEVBR)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase 3
      Short name=HMG-CoA reductase 3
    EC=1.1.1.34

Gene names

Name:

HMGR3

Organism

Hevea brasiliensis (Para rubber tree)

Taxonomic identifier

3981 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Euphorbiaceae › Crotonoideae › Micrandreae › Hevea

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Plastid membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Plastid
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: InterPro mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plastid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	3-hydroxy-3-methylglutaryl-coenzyme A reductase 3		PRO_0000114442
Regions
	Transmembrane	36 – 59	24	By similarity
	Transmembrane	87 – 107	21	By similarity
	Region	108 – 170	63	Linker By similarity
	Region	171 – 586	416	Catalytic By similarity
	Region	172 – 586	415	Catalytic By similarity
Sites
	Active site	265	1	Charge relay system By similarity
	Active site	397	1	Charge relay system By similarity
	Active site	473	1	Charge relay system By similarity
	Active site	571	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	575	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q00583-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 0B9D6DCC5979B456	FASTA	586	62,970
	10 20 30 40 50 60 MDEVRRRPPK HIVRKDHDGE VLNSFSHGHH LPPLKPSDYS LPLSLYLANA LVFSLFFSVA 70 80 90 100 110 120 YFLLHRWREK IRKSTPLHIV TFPEIAALIC LVASVIYLLG FFGIGFVHSF SRASTDSWDV 130 140 150 160 170 180 EEYDDDNIII KEDTRPTGAC AAPSLDCSLS LPTKIHAPIV STTTTSTLSD DDEQIIKSVV 190 200 210 220 230 240 SGSIPSYSLE SKLGNCKRAA LIRRETLQRM SGRSLEGLPL DGFDYESILG QCCEMAIGYV 250 260 270 280 290 300 QIPVGIAGPL LLDGKEYTVP MATTEGCLVA SANRGCKAIY ASGGATSVLL RDGMTRAPVV 310 320 330 340 350 360 RFPTAKRAAD LKFFMEDPDN FDTIAVVFNK SSRFARLQSV QCAIAGKNLY MRFSCSTGDA 370 380 390 400 410 420 MGMNMVSKAV QNVIDYLQND FPDMDVIGLT GNFCADKKAA AVNWIEGRGK SVVCEAIIKE 430 440 450 460 470 480 EVVKKVLKTN VAALVELNMI KNLTGSAVAG SLGGFNAHAS NMVTAVYIAT GQDPAQNVES 490 500 510 520 530 540 SHCITMMEAV NDGKDLHISV SMPSIELGTV GGGTQLASQS ACLNLLGVKG ASKDSPGSNS 550 560 570 580 RLLATIVAGS VLAGELSLMS AIAAGQLVNS HMKYNRSAKD VSKITF

References

[1]	"Three genes encode 3-hydroxy-3-methylglutaryl-coenzyme A reductase in Hevea brasiliensis: hmg1 and hmg3 are differentially expressed." Chye M.L., Tan C.T., Chua N.H. Plant Mol. Biol. 19:473-484(1992) [PubMed: 1377968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M74800 mRNA. Translation: AAA33360.1. M74798 Genomic DNA. Translation: AAA33358.1. M74799 Genomic DNA. Translation: AAA33359.1.
PIR	S22521.
3D structure databases
HSSP	HSSP built from PDB template 1HWI based on UniProtKB P04035.
ModBase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH3_HEVBR

Accession

Primary (citable) accession number: Q00583

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 25, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)