Reviewed, UniProtKB/Swiss-Prot Q00582 (GTR1_YEAST)

Names and origin

Protein names

Recommended name:
    GTP-binding protein GTR1

Gene names

Name:	GTR1
Ordered Locus Names:	YML121W
ORF Names:	YM7056.05

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTPase component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Functionally associated with the inorganic phosphate transporter PHO84. It is probably required for modulation of PHO84 or involved in its intracellular localization.
Subunit structure	Component of the GSE complex composed of GTR1, GTR2, SLM4, MEH1 and LTV1.
Subcellular location	Vacuole membrane.
Miscellaneous	Present with 907 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the GTR/RAG GTP-binding protein family.

Ontologies

Keywords
Biological process	Phosphate transport Protein transport Transport
Cellular component	Membrane Vacuole
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin silencing at telomere Inferred from mutant phenotype. Source: SGD phosphate transport Ref.1 Inferred from mutant phenotype. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase I promoter Inferred from mutant phenotype. Source: SGD transcription from RNA polymerase III promoter Inferred from mutant phenotype. Source: SGD
Cellular component	GSE complex Ref.5 Inferred from direct assay. Source: SGD late endosome membrane Ref.5 Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD vacuolar membrane Inferred from direct assay. Source: SGD
Molecular function	GDP binding Inferred from direct assay. Source: SGD GTP binding Inferred from direct assay. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-7954,EBI-7954
MEH1	Q02205	1	EBI-7954,EBI-27062
PIB2	P53191	1	EBI-7954,EBI-13400
SLM4	P38247	1	EBI-7954,EBI-21507
VAC8	P39968	1	EBI-7954,EBI-20212
VMA4	P22203	1	EBI-7954,EBI-20268
VMA8	P32610	1	EBI-7954,EBI-20264

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	GTP-binding protein GTR1		PRO_0000122486
Regions
	Nucleotide binding	13 – 20	8	GTP Potential
	Nucleotide binding	61 – 65	5	GTP By similarity
	Nucleotide binding	126 – 129	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00582-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: B79CE7FE7AD2FDBE	FASTA	310	35,847
	10 20 30 40 50 60 MSSNNRKKLL LMGRSGSGKS SMRSIIFSNY SAFDTRRLGA TIDVEHSHLR FLGNMTLNLW 70 80 90 100 110 120 DCGGQDVFME NYFTKQKDHI FQMVQVLIHV FDVESTEVLK DIEIFAKALK QLRKYSPDAK 130 140 150 160 170 180 IFVLLHKMDL VQLDKREELF QIMMKNLSET SSEFGFPNLI GFPTSIWDES LYKAWSQIVC 190 200 210 220 230 240 SLIPNMSNHQ SNLKKFKEIM NALEIILFER TTFLVICSSN GENSNENHDS SDNNNVLLDP 250 260 270 280 290 300 KRFEKISNIM KNFKQSCTKL KSGFKTLILN NNIYVSELSS NMVCFIVLKD MNIPQELVLE 310 NIKKAKEFFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Putative GTP-binding protein, Gtr1, associated with the function of the Pho84 inorganic phosphate transporter in Saccharomyces cerevisiae." Bun-Ya M., Harashima S., Oshima Y. Mol. Cell. Biol. 12:2958-2966(1992) [PubMed: 1620108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast." Gao M., Kaiser C.A. Nat. Cell Biol. 8:657-667(2006) [PubMed: 16732272] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE GSE COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10018 Genomic DNA. Translation: BAA00907.1. Z49218 Genomic DNA. Translation: CAA89159.1. AY692917 Genomic DNA. Translation: AAT92936.1.
PIR	S31303.
RefSeq	NP_013585.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1358N.
IntAct	Q00582. 16 interactions.
STRING	Q00582.
Genome annotation databases
Ensembl	YML121W; YML121W; YML121W; Saccharomyces cerevisiae. [Genome view]
GeneID	854918.
KEGG	sce:YML121W.
NMPDR	fig|4932.3.peg.4621.
Organism-specific databases
CYGD	YML121w.
SGD	S000004590. GTR1.
Phylogenomic databases
HOGENOM	Q00582.
OMA	EVILFER
OrthoDB	EOG9QRJMC
Gene expression databases
ArrayExpress	Q00582.
Genevestigator	Q00582.
GermOnline	YML121W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006762. Gtr1_RagA. [Graphical view]
PANTHER	PTHR11259. Gtr1_RagA. 1 hit.
Pfam	PF04670. Gtr1_RagA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977926.

Entry information

Entry name

GTR1_YEAST

Accession

Primary (citable) accession number: Q00582

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 24, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)