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Q00577

- PURA_HUMAN

UniProt

Q00577 - PURA_HUMAN

Protein

Transcriptional activator protein Pur-alpha

Gene

PURA

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination.

    GO - Molecular functioni

    1. double-stranded telomeric DNA binding Source: UniProtKB
    2. poly(A) RNA binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. purine-rich negative regulatory element binding Source: Ensembl
    5. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: ProtInc
    6. single-stranded DNA binding Source: UniProtKB
    7. transcription factor binding Source: UniProtKB
    8. translation repressor activity, nucleic acid binding Source: Ensembl

    GO - Biological processi

    1. DNA replication initiation Source: ProtInc
    2. DNA unwinding involved in DNA replication Source: UniProtKB
    3. negative regulation of transcription, DNA-templated Source: Ensembl
    4. nervous system development Source: Ensembl
    5. positive regulation of cell proliferation Source: Ensembl
    6. regulation of transcription from RNA polymerase II promoter Source: GOC
    7. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    SignaLinkiQ00577.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional activator protein Pur-alpha
    Alternative name(s):
    Purine-rich single-stranded DNA-binding protein alpha
    Gene namesi
    Name:PURA
    Synonyms:PUR1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:9701. PURA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. dendrite Source: Ensembl
    3. DNA replication factor A complex Source: UniProtKB
    4. neuronal cell body Source: Ensembl
    5. nuclear chromosome, telomeric region Source: UniProtKB
    6. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34045.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 322322Transcriptional activator protein Pur-alphaPRO_0000097107Add
    BLAST

    Proteomic databases

    MaxQBiQ00577.
    PaxDbiQ00577.
    PeptideAtlasiQ00577.
    PRIDEiQ00577.

    PTM databases

    PhosphoSiteiQ00577.

    Expressioni

    Gene expression databases

    BgeeiQ00577.
    CleanExiHS_PURA.
    GenevestigatoriQ00577.

    Interactioni

    Subunit structurei

    Homodimer, heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    P030702EBI-1045860,EBI-617698From a different organism.
    P030724EBI-1045860,EBI-8658901From a different organism.
    RB1P064006EBI-1045860,EBI-491274

    Protein-protein interaction databases

    BioGridi111772. 26 interactions.
    IntActiQ00577. 9 interactions.
    MINTiMINT-112751.
    STRINGi9606.ENSP00000332706.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00577.
    SMRiQ00577. Positions 59-277.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi11 – 5343Gly-richAdd
    BLAST
    Compositional biasi293 – 32230Gln/Glu-rich (part of the transcriptional activation domain)Add
    BLAST

    Sequence similaritiesi

    Belongs to the PUR DNA-binding protein family.Curated

    Phylogenomic databases

    eggNOGiNOG312871.
    HOGENOMiHOG000232132.
    HOVERGENiHBG006888.
    InParanoidiQ00577.
    OMAiREKRACE.
    OrthoDBiEOG78D7M4.
    PhylomeDBiQ00577.
    TreeFamiTF313701.

    Family and domain databases

    InterProiIPR006628. PUR_DNA_RNA-bd.
    [Graphical view]
    PANTHERiPTHR12611. PTHR12611. 1 hit.
    PfamiPF04845. PurA. 1 hit.
    [Graphical view]
    SMARTiSM00712. PUR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00577-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADRDSGSEQ GGAALGSGGS LGHPGSGSGS GGGGGGGGGG GGSGGGGGGA    50
    PGGLQHETQE LASKRVDIQN KRFYLDVKQN AKGRFLKIAE VGAGGNKSRL 100
    TLSMSVAVEF RDYLGDFIEH YAQLGPSQPP DLAQAQDEPR RALKSEFLVR 150
    ENRKYYMDLK ENQRGRFLRI RQTVNRGPGL GSTQGQTIAL PAQGLIEFRD 200
    ALAKLIDDYG VEEEPAELPE GTSLTVDNKR FFFDVGSNKY GVFMRVSEVK 250
    PTYRNSITVP YKVWAKFGHT FCKYSEEMKK IQEKQREKRA ACEQLHQQQQ 300
    QQQEETAAAT LLLQGEEEGE ED 322
    Length:322
    Mass (Da):34,911
    Last modified:February 1, 1996 - v2
    Checksum:i797968504D01B356
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96684 mRNA. Translation: AAA60229.1.
    U02098 mRNA. No translation available.
    CCDSiCCDS4220.1.
    PIRiA45036.
    RefSeqiNP_005850.1. NM_005859.4.
    UniGeneiHs.443121.
    Hs.733524.

    Genome annotation databases

    EnsembliENST00000331327; ENSP00000332706; ENSG00000185129.
    GeneIDi5813.
    KEGGihsa:5813.
    UCSCiuc003lfa.3. human.

    Polymorphism databases

    DMDMi1346918.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96684 mRNA. Translation: AAA60229.1 .
    U02098 mRNA. No translation available.
    CCDSi CCDS4220.1.
    PIRi A45036.
    RefSeqi NP_005850.1. NM_005859.4.
    UniGenei Hs.443121.
    Hs.733524.

    3D structure databases

    ProteinModelPortali Q00577.
    SMRi Q00577. Positions 59-277.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111772. 26 interactions.
    IntActi Q00577. 9 interactions.
    MINTi MINT-112751.
    STRINGi 9606.ENSP00000332706.

    PTM databases

    PhosphoSitei Q00577.

    Polymorphism databases

    DMDMi 1346918.

    Proteomic databases

    MaxQBi Q00577.
    PaxDbi Q00577.
    PeptideAtlasi Q00577.
    PRIDEi Q00577.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000331327 ; ENSP00000332706 ; ENSG00000185129 .
    GeneIDi 5813.
    KEGGi hsa:5813.
    UCSCi uc003lfa.3. human.

    Organism-specific databases

    CTDi 5813.
    GeneCardsi GC05P139473.
    HGNCi HGNC:9701. PURA.
    MIMi 600473. gene.
    neXtProti NX_Q00577.
    PharmGKBi PA34045.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG312871.
    HOGENOMi HOG000232132.
    HOVERGENi HBG006888.
    InParanoidi Q00577.
    OMAi REKRACE.
    OrthoDBi EOG78D7M4.
    PhylomeDBi Q00577.
    TreeFami TF313701.

    Enzyme and pathway databases

    SignaLinki Q00577.

    Miscellaneous databases

    GeneWikii PURA.
    GenomeRNAii 5813.
    NextBioi 22642.
    PROi Q00577.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q00577.
    CleanExi HS_PURA.
    Genevestigatori Q00577.

    Family and domain databases

    InterProi IPR006628. PUR_DNA_RNA-bd.
    [Graphical view ]
    PANTHERi PTHR12611. PTHR12611. 1 hit.
    Pfami PF04845. PurA. 1 hit.
    [Graphical view ]
    SMARTi SM00712. PUR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of cDNA comprising the human pur gene and sequence-specific single-stranded-DNA-binding properties of the encoded protein."
      Bergemann A.D., Ma Z.-W., Johnson E.M.
      Mol. Cell. Biol. 12:5673-5682(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Fetal liver.
    2. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPURA_HUMAN
    AccessioniPrimary (citable) accession number: Q00577
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3