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Protein

Transcriptional activator protein Pur-alpha

Gene

PURA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination.

GO - Molecular functioni

  1. double-stranded telomeric DNA binding Source: UniProtKB
  2. poly(A) RNA binding Source: UniProtKB
  3. purine-rich negative regulatory element binding Source: Ensembl
  4. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: ProtInc
  5. single-stranded DNA binding Source: UniProtKB
  6. transcription factor binding Source: UniProtKB
  7. translation repressor activity, nucleic acid binding Source: Ensembl

GO - Biological processi

  1. DNA replication initiation Source: ProtInc
  2. DNA unwinding involved in DNA replication Source: UniProtKB
  3. negative regulation of transcription, DNA-templated Source: Ensembl
  4. nervous system development Source: Ensembl
  5. positive regulation of cell proliferation Source: Ensembl
  6. regulation of transcription from RNA polymerase II promoter Source: GOC
  7. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiQ00577.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator protein Pur-alpha
Alternative name(s):
Purine-rich single-stranded DNA-binding protein alpha
Gene namesi
Name:PURA
Synonyms:PUR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:9701. PURA.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. dendrite Source: Ensembl
  3. DNA replication factor A complex Source: UniProtKB
  4. neuronal cell body Source: Ensembl
  5. nuclear chromosome, telomeric region Source: UniProtKB
  6. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34045.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 322321Transcriptional activator protein Pur-alphaPRO_0000097107Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ00577.
PaxDbiQ00577.
PeptideAtlasiQ00577.
PRIDEiQ00577.

PTM databases

PhosphoSiteiQ00577.

Expressioni

Gene expression databases

BgeeiQ00577.
CleanExiHS_PURA.
GenevestigatoriQ00577.

Interactioni

Subunit structurei

Homodimer, heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
P030702EBI-1045860,EBI-617698From a different organism.
P030724EBI-1045860,EBI-8658901From a different organism.
RB1P064006EBI-1045860,EBI-491274

Protein-protein interaction databases

BioGridi111772. 31 interactions.
IntActiQ00577. 9 interactions.
MINTiMINT-112751.
STRINGi9606.ENSP00000332706.

Structurei

3D structure databases

ProteinModelPortaliQ00577.
SMRiQ00577. Positions 59-277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 5343Gly-richAdd
BLAST
Compositional biasi293 – 32230Gln/Glu-rich (part of the transcriptional activation domain)Add
BLAST

Sequence similaritiesi

Belongs to the PUR DNA-binding protein family.Curated

Phylogenomic databases

eggNOGiNOG312871.
GeneTreeiENSGT00390000015406.
HOGENOMiHOG000232132.
HOVERGENiHBG006888.
InParanoidiQ00577.
OMAiVPYKVWS.
OrthoDBiEOG78D7M4.
PhylomeDBiQ00577.
TreeFamiTF313701.

Family and domain databases

InterProiIPR006628. PUR_DNA_RNA-bd.
[Graphical view]
PANTHERiPTHR12611. PTHR12611. 1 hit.
PfamiPF04845. PurA. 1 hit.
[Graphical view]
SMARTiSM00712. PUR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00577-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRDSGSEQ GGAALGSGGS LGHPGSGSGS GGGGGGGGGG GGSGGGGGGA
60 70 80 90 100
PGGLQHETQE LASKRVDIQN KRFYLDVKQN AKGRFLKIAE VGAGGNKSRL
110 120 130 140 150
TLSMSVAVEF RDYLGDFIEH YAQLGPSQPP DLAQAQDEPR RALKSEFLVR
160 170 180 190 200
ENRKYYMDLK ENQRGRFLRI RQTVNRGPGL GSTQGQTIAL PAQGLIEFRD
210 220 230 240 250
ALAKLIDDYG VEEEPAELPE GTSLTVDNKR FFFDVGSNKY GVFMRVSEVK
260 270 280 290 300
PTYRNSITVP YKVWAKFGHT FCKYSEEMKK IQEKQREKRA ACEQLHQQQQ
310 320
QQQEETAAAT LLLQGEEEGE ED
Length:322
Mass (Da):34,911
Last modified:February 1, 1996 - v2
Checksum:i797968504D01B356
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96684 mRNA. Translation: AAA60229.1.
U02098 mRNA. No translation available.
CCDSiCCDS4220.1.
PIRiA45036.
RefSeqiNP_005850.1. NM_005859.4.
UniGeneiHs.443121.
Hs.733524.

Genome annotation databases

EnsembliENST00000331327; ENSP00000332706; ENSG00000185129.
GeneIDi5813.
KEGGihsa:5813.
UCSCiuc003lfa.3. human.

Polymorphism databases

DMDMi1346918.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96684 mRNA. Translation: AAA60229.1.
U02098 mRNA. No translation available.
CCDSiCCDS4220.1.
PIRiA45036.
RefSeqiNP_005850.1. NM_005859.4.
UniGeneiHs.443121.
Hs.733524.

3D structure databases

ProteinModelPortaliQ00577.
SMRiQ00577. Positions 59-277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111772. 31 interactions.
IntActiQ00577. 9 interactions.
MINTiMINT-112751.
STRINGi9606.ENSP00000332706.

PTM databases

PhosphoSiteiQ00577.

Polymorphism databases

DMDMi1346918.

Proteomic databases

MaxQBiQ00577.
PaxDbiQ00577.
PeptideAtlasiQ00577.
PRIDEiQ00577.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000331327; ENSP00000332706; ENSG00000185129.
GeneIDi5813.
KEGGihsa:5813.
UCSCiuc003lfa.3. human.

Organism-specific databases

CTDi5813.
GeneCardsiGC05P139473.
HGNCiHGNC:9701. PURA.
MIMi600473. gene.
neXtProtiNX_Q00577.
PharmGKBiPA34045.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG312871.
GeneTreeiENSGT00390000015406.
HOGENOMiHOG000232132.
HOVERGENiHBG006888.
InParanoidiQ00577.
OMAiVPYKVWS.
OrthoDBiEOG78D7M4.
PhylomeDBiQ00577.
TreeFamiTF313701.

Enzyme and pathway databases

SignaLinkiQ00577.

Miscellaneous databases

GeneWikiiPURA.
GenomeRNAii5813.
NextBioi22642.
PROiQ00577.
SOURCEiSearch...

Gene expression databases

BgeeiQ00577.
CleanExiHS_PURA.
GenevestigatoriQ00577.

Family and domain databases

InterProiIPR006628. PUR_DNA_RNA-bd.
[Graphical view]
PANTHERiPTHR12611. PTHR12611. 1 hit.
PfamiPF04845. PurA. 1 hit.
[Graphical view]
SMARTiSM00712. PUR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of cDNA comprising the human pur gene and sequence-specific single-stranded-DNA-binding properties of the encoded protein."
    Bergemann A.D., Ma Z.-W., Johnson E.M.
    Mol. Cell. Biol. 12:5673-5682(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fetal liver.
  2. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  3. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  4. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPURA_HUMAN
AccessioniPrimary (citable) accession number: Q00577
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 1, 1996
Last modified: March 4, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.