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Protein

Methyl-CpG-binding protein 2

Gene

Mecp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi185 – 197A.T hook 1Add BLAST13
DNA bindingi265 – 277A.T hook 2Add BLAST13

GO - Molecular functioni

  • chromatin DNA binding Source: RGD
  • double-stranded methylated DNA binding Source: RGD
  • enzyme binding Source: RGD
  • four-way junction DNA binding Source: RGD
  • histone deacetylase binding Source: RGD
  • methyl-CpG binding Source: RGD
  • unmethylated CpG binding Source: RGD

GO - Biological processi

  • heart development Source: RGD
  • locomotory behavior Source: GO_Central
  • negative regulation of astrocyte differentiation Source: RGD
  • negative regulation of transcription, DNA-templated Source: RGD
  • negative regulation of transcription from RNA polymerase II promoter Source: RGD
  • regulation of DNA methylation Source: RGD
  • response to estradiol Source: RGD
  • response to ionizing radiation Source: RGD
  • response to lead ion Source: RGD
  • response to organic cyclic compound Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-CpG-binding protein 2
Short name:
MeCp-2 protein
Short name:
MeCp2
Gene namesi
Name:Mecp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3075. Mecp2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
  • heterochromatin Source: GO_Central
  • nuclear chromatin Source: RGD
  • nucleus Source: RGD
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000963481 – 492Methyl-CpG-binding protein 2Add BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineCombined sources1
Modified residuei80PhosphoserineCombined sources1 Publication1
Modified residuei116PhosphoserineBy similarity1
Modified residuei162Omega-N-methylarginineBy similarity1
Modified residuei216PhosphoserineBy similarity1
Modified residuei229PhosphoserineCombined sources1 Publication1
Modified residuei321N6-acetyllysineBy similarity1
Modified residuei421Phosphoserine; by CaMK21 Publication1
Modified residuei424PhosphoserineBy similarity1
Modified residuei455N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated on Ser-421 by CaMK2 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ00566.
PRIDEiQ00566.

PTM databases

iPTMnetiQ00566.
PhosphoSitePlusiQ00566.

Expressioni

Tissue specificityi

Present in all adult somatic tissues tested.

Inductioni

In brain, by repeated injections of fluoxetine or cocaine.1 Publication

Interactioni

Subunit structurei

Interacts with FNBP3 (By similarity). Interacts with CDKL5 (By similarity). Interacts with ATRX; MECP2 recruits ATRX to pericentric heterochromatin in neuronal cells (PubMed:17296936). Interacts with NCOR2 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ATRXP461006EBI-9396907,EBI-396461From a different organism.

GO - Molecular functioni

  • enzyme binding Source: RGD
  • histone deacetylase binding Source: RGD

Protein-protein interaction databases

BioGridi248038. 1 interactor.
DIPiDIP-60875N.
IntActiQ00566. 2 interactors.
STRINGi10116.ENSRNOP00000053166.

Structurei

3D structure databases

ProteinModelPortaliQ00566.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 162MBDPROSITE-ProRule annotationAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni269 – 309Interaction with NCOR2By similarityAdd BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi366 – 372His-rich7
Compositional biasi379 – 403Pro-richAdd BLAST25

Sequence similaritiesi

Contains 2 A.T hook DNA-binding domains.Curated
Contains 1 MBD (methyl-CpG-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4161. Eukaryota.
ENOG41126JX. LUCA.
HOGENOMiHOG000015809.
HOVERGENiHBG052445.
InParanoidiQ00566.
KOiK11588.
PhylomeDBiQ00566.
TreeFamiTF332974.

Family and domain databases

Gene3Di3.30.890.10. 1 hit.
InterProiIPR016177. DNA-bd_dom.
IPR017353. Me_CpG-bd_MeCP2.
IPR001739. Methyl_CpG_DNA-bd.
[Graphical view]
PfamiPF01429. MBD. 1 hit.
[Graphical view]
PIRSFiPIRSF038006. Methyl_CpG_bd_MeCP2. 1 hit.
SMARTiSM00391. MBD. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS50982. MBD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q00566-1) [UniParc]FASTAAdd to basket
Also known as: e2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVAGMLGLRK EKSEDQDLQG LKEKPLKFKK VKKDKKEDKE GKHEPLQPSA
60 70 80 90 100
HHSAEPAEAG KAETSESSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL
110 120 130 140 150
PEGWTRKLKQ RKSGRSAGKY DVYLINPQGK AFRSKVELIA YFEKVGDTSL
160 170 180 190 200
DPNDFDFTVT GRGSPSRREQ KPPKKPKSPK APGTGRGRGR PKGSGTGRPK
210 220 230 240 250
AAASEGVQVK RVLEKSPGKL LVKMPFQASP GGKGEGGGAT TSAQVMVIKR
260 270 280 290 300
PGRKRKAEAD PQAIPKKRGR KPGSVVAAAA AEAKKKAVKE SSIRSVQETV
310 320 330 340 350
LPIKKRKTRE TVSIEVKEVV KPLLVSTLGE KSGKGLKTCK SPGRKSKESS
360 370 380 390 400
PKGRSSSASS PPKKEHHHHH HHAESPKAPM PLLPPPPPPE PQSSEDPISP
410 420 430 440 450
PEPQDLSSSI CKEEKMPRAG SLESDGCPKE PAKTQPMVAA AATTTTTTTT
460 470 480 490
TVAEKYKHRG EGERKDIVSS SMPRPNREEP VDSRTPVTER VS
Length:492
Mass (Da):53,047
Last modified:April 1, 1993 - v1
Checksum:iA67E705C68BA2D38
GO
Isoform B (identifier: Q00566-2) [UniParc]FASTAAdd to basket
Also known as: e1

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MVAGMLGLRK → MAAAAAAAAAAAAAAAAAAAAAAAAPSGGGGGEEERLE

Show »
Length:520
Mass (Da):55,082
Checksum:i02800646B6DF90D8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10K → E in ABI55238 (PubMed:16670375).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0229501 – 10MVAGMLGLRK → MAAAAAAAAAAAAAAAAAAA AAAAAPSGGGGGEEERLE in isoform B. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94064 mRNA. Translation: AAA41584.1.
DQ897368 mRNA. Translation: ABI55237.1.
DQ897369 mRNA. Translation: ABI55238.1.
PIRiA41907.
RefSeqiNP_073164.2. NM_022673.2.
XP_006229629.1. XM_006229567.3.
UniGeneiRn.38416.
Rn.9680.

Genome annotation databases

GeneIDi29386.
KEGGirno:29386.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94064 mRNA. Translation: AAA41584.1.
DQ897368 mRNA. Translation: ABI55237.1.
DQ897369 mRNA. Translation: ABI55238.1.
PIRiA41907.
RefSeqiNP_073164.2. NM_022673.2.
XP_006229629.1. XM_006229567.3.
UniGeneiRn.38416.
Rn.9680.

3D structure databases

ProteinModelPortaliQ00566.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248038. 1 interactor.
DIPiDIP-60875N.
IntActiQ00566. 2 interactors.
STRINGi10116.ENSRNOP00000053166.

PTM databases

iPTMnetiQ00566.
PhosphoSitePlusiQ00566.

Proteomic databases

PaxDbiQ00566.
PRIDEiQ00566.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29386.
KEGGirno:29386.

Organism-specific databases

CTDi4204.
RGDi3075. Mecp2.

Phylogenomic databases

eggNOGiKOG4161. Eukaryota.
ENOG41126JX. LUCA.
HOGENOMiHOG000015809.
HOVERGENiHBG052445.
InParanoidiQ00566.
KOiK11588.
PhylomeDBiQ00566.
TreeFamiTF332974.

Miscellaneous databases

PROiQ00566.

Family and domain databases

Gene3Di3.30.890.10. 1 hit.
InterProiIPR016177. DNA-bd_dom.
IPR017353. Me_CpG-bd_MeCP2.
IPR001739. Methyl_CpG_DNA-bd.
[Graphical view]
PfamiPF01429. MBD. 1 hit.
[Graphical view]
PIRSFiPIRSF038006. Methyl_CpG_bd_MeCP2. 1 hit.
SMARTiSM00391. MBD. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS50982. MBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMECP2_RAT
AccessioniPrimary (citable) accession number: Q00566
Secondary accession number(s): Q09HV7, Q09HV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.