Q00566 (MECP2_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 106.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Methyl-CpG-binding protein 2 Short name=MeCp-2 protein Short name=MeCp2 | ||
| Gene names |
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| Organism | Rattus norvegicus (Rat) [Reference proteome] | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 492 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A By similarity. |
| Subunit structure | Interacts with FNBP3 By similarity. Interacts with CDKL5 By similarity. |
| Subcellular location | Nucleus. Note: Colocalized with methyl-CpG in the genome. Ref.2 |
| Tissue specificity | Present in all adult somatic tissues tested. |
| Induction | In brain, by repeated injections of fluoxetine or cocaine. Ref.2 |
| Post-translational modification | Phosphorylated on Ser-421 by CaMK2 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. Ref.3 |
| Sequence similarities | Contains 2 A.T hook DNA-binding domains. Contains 1 MBD (methyl-CpG-binding) domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform A (identifier: Q00566-1) Also known as: e2; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform B (identifier: Q00566-2) Also known as: e1; The sequence of this isoform differs from the canonical sequence as follows: 1-10: MVAGMLGLRK → MAAAAAAAAAAAAAAAAAAAAAAAAPSGGGGGEEERLE |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 492 | 492 | Methyl-CpG-binding protein 2 | PRO_0000096348 | |||||
Regions | |||||||||
| Domain | 90 – 162 | 73 | MBD | ||||||
| DNA binding | 185 – 197 | 13 | A.T hook 1 | ||||||
| DNA binding | 265 – 277 | 13 | A.T hook 2 | ||||||
| Compositional bias | 366 – 372 | 7 | His-rich | ||||||
| Compositional bias | 379 – 403 | 25 | Pro-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 80 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 116 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 216 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 229 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 421 | 1 | Phosphoserine; by CaMK2 Ref.3 | ||||||
| Modified residue | 424 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 455 | 1 | N6-acetyllysine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 10 | 10 | MVAGMLGLRK → MAAAAAAAAAAAAAAAAAAA AAAAAPSGGGGGEEERLE in isoform B. | VSP_022950 | |||||
Experimental info | |||||||||
| Sequence conflict | 10 | 1 | K → E in ABI55238. Ref.2 | ||||||
Sequences
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References
| [1] | "Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA." Lewis J.D., Meehan R.R., Henzel W.J., Maurer-Fogy I., Jeppesen P., Klein F., Bird A. Cell 69:905-914(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), PARTIAL PROTEIN SEQUENCE. Strain: Wistar. Tissue: Brain and Corpus striatum. |
| [2] | "Fluoxetine and cocaine induce the epigenetic factors MeCP2 and MBD1 in adult rat brain." Cassel S., Carouge D., Gensburger C., Anglard P., Burgun C., Dietrich J.-B., Aunis D., Zwiller J. Mol. Pharmacol. 70:487-492(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), SUBCELLULAR LOCATION, INDUCTION BY FLUOXETINE AND COCAINE. Strain: Wistar. Tissue: Brain cortex. |
| [3] | "Brain-specific phosphorylation of MeCP2 regulates activity-dependent Bdnf transcription, dendritic growth, and spine maturation." Zhou Z., Hong E.J., Cohen S., Zhao W.-N., Ho H.H., Schmidt L., Chen W.G., Lin Y., Savner E., Griffith E.C., Hu L., Steen J.A.J., Weitz C.J., Greenberg M.E. Neuron 52:255-269(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-80; SER-229 AND SER-421, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M94064 mRNA. Translation: AAA41584.1. DQ897368 mRNA. Translation: ABI55237.1. DQ897369 mRNA. Translation: ABI55238.1. |
| IPI | IPI00798628. IPI00829488. |
| PIR | A41907. |
| RefSeq | NP_073164.2. NM_022673.2. |
| UniGene | Rn.9680. |
3D structure databases | |
| ProteinModelPortal | Q00566. |
| SMR | Q00566. Positions 71-195. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q00566. |
Proteomic databases | |
| PaxDb | Q00566. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 29386. |
| KEGG | rno:29386. |
Organism-specific databases | |
| CTD | 4204. |
| RGD | 3075. Mecp2. |
Phylogenomic databases | |
| eggNOG | NOG237320. |
| HOGENOM | HOG000015809. |
| HOVERGEN | HBG052445. |
| InParanoid | Q00566. |
| KO | K11588. |
| OrthoDB | EOG40VVQ3. |
Gene expression databases | |
| ArrayExpress | Q00566. |
| Genevestigator | Q00566. |
| GermOnline | ENSRNOG00000037262. Rattus norvegicus. |
Family and domain databases | |
| Gene3D | 3.30.890.10. 1 hit. |
| InterPro | IPR017956. AT_hook_DNA-bd_motif. IPR016177. DNA-bd_integrase-typ. IPR017353. Me_CpG-bd_MeCP2. IPR001739. Methyl_CpG_DNA-bd. [Graphical view] |
| Pfam | PF01429. MBD. 1 hit. [Graphical view] |
| PIRSF | PIRSF038006. Methyl_CpG_bd_MeCP2. 1 hit. |
| SMART | SM00384. AT_hook. 2 hits. SM00391. MBD. 1 hit. [Graphical view] |
| SUPFAM | SSF54171. DNA-binding_integrase-type. 1 hit. |
| PROSITE | PS50982. MBD. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 608987. |
Entry information
| Entry name | MECP2_RAT | ||||||||
| Accession | Primary (citable) accession number: Q00566 Secondary accession number(s): Q09HV7, Q09HV8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |