Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q00566 (MECP2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-CpG-binding protein 2

Short name=MeCp-2 protein
Short name=MeCp2
Gene names
Name:Mecp2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC) By similarity.

Subunit structure

Interacts with FNBP3 By similarity. Interacts with CDKL5 By similarity.

Subcellular location

Nucleus. Note: Colocalized with methyl-CpG in the genome. Ref.2

Tissue specificity

Present in all adult somatic tissues tested.

Induction

In brain, by repeated injections of fluoxetine or cocaine. Ref.2

Post-translational modification

Phosphorylated on Ser-421 by CaMK2 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. Ref.3

Sequence similarities

Contains 2 A.T hook DNA-binding domains.

Contains 1 MBD (methyl-CpG-binding) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandDNA-binding
   Molecular functionRepressor
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 9038338. Source: RGD

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 9038338. Source: RGD

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytosol

Inferred from direct assay PubMed 17052801. Source: RGD

nuclear chromatin

Inferred from direct assay PubMed 9038338. Source: RGD

nucleus

Inferred from direct assay PubMed 11844796PubMed 17052801. Source: RGD

protein complex

Inferred from direct assay PubMed 12473678. Source: RGD

   Molecular_functionchromatin DNA binding

Inferred from direct assay PubMed 9038338. Source: RGD

double-stranded methylated DNA binding

Inferred from direct assay PubMed 16314321PubMed 9038338. Source: RGD

enzyme binding

Inferred from physical interaction PubMed 12473678. Source: RGD

four-way junction DNA binding

Inferred from direct assay PubMed 16314321. Source: RGD

methyl-CpG binding

Inferred from direct assay PubMed 11844796PubMed 12473678. Source: RGD

unmethylated CpG binding

Inferred from direct assay PubMed 16314321. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q00566-1)

Also known as: e2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q00566-2)

Also known as: e1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MVAGMLGLRK → MAAAAAAAAAAAAAAAAAAAAAAAAPSGGGGGEEERLE

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Methyl-CpG-binding protein 2
PRO_0000096348

Regions

Domain90 – 16273MBD
DNA binding185 – 19713A.T hook 1
DNA binding265 – 27713A.T hook 2
Compositional bias366 – 3727His-rich
Compositional bias379 – 40325Pro-rich

Amino acid modifications

Modified residue801Phosphoserine Ref.3
Modified residue1161Phosphoserine By similarity
Modified residue2161Phosphoserine By similarity
Modified residue2291Phosphoserine Ref.3
Modified residue3211N6-acetyllysine By similarity
Modified residue4211Phosphoserine; by CaMK2 Ref.3
Modified residue4241Phosphoserine By similarity
Modified residue4551N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 1010MVAGMLGLRK → MAAAAAAAAAAAAAAAAAAA AAAAAPSGGGGGEEERLE in isoform B.
VSP_022950

Experimental info

Sequence conflict101K → E in ABI55238. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform A (e2) [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: A67E705C68BA2D38

FASTA49253,047
        10         20         30         40         50         60 
MVAGMLGLRK EKSEDQDLQG LKEKPLKFKK VKKDKKEDKE GKHEPLQPSA HHSAEPAEAG 

        70         80         90        100        110        120 
KAETSESSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY 

       130        140        150        160        170        180 
DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ KPPKKPKSPK 

       190        200        210        220        230        240 
APGTGRGRGR PKGSGTGRPK AAASEGVQVK RVLEKSPGKL LVKMPFQASP GGKGEGGGAT 

       250        260        270        280        290        300 
TSAQVMVIKR PGRKRKAEAD PQAIPKKRGR KPGSVVAAAA AEAKKKAVKE SSIRSVQETV 

       310        320        330        340        350        360 
LPIKKRKTRE TVSIEVKEVV KPLLVSTLGE KSGKGLKTCK SPGRKSKESS PKGRSSSASS 

       370        380        390        400        410        420 
PPKKEHHHHH HHAESPKAPM PLLPPPPPPE PQSSEDPISP PEPQDLSSSI CKEEKMPRAG 

       430        440        450        460        470        480 
SLESDGCPKE PAKTQPMVAA AATTTTTTTT TVAEKYKHRG EGERKDIVSS SMPRPNREEP 

       490 
VDSRTPVTER VS 

« Hide

Isoform B (e1) [UniParc].

Checksum: 02800646B6DF90D8
Show »

FASTA52055,082

References

[1]"Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA."
Lewis J.D., Meehan R.R., Henzel W.J., Maurer-Fogy I., Jeppesen P., Klein F., Bird A.
Cell 69:905-914(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), PARTIAL PROTEIN SEQUENCE.
Strain: Wistar.
Tissue: Brain and Corpus striatum.
[2]"Fluoxetine and cocaine induce the epigenetic factors MeCP2 and MBD1 in adult rat brain."
Cassel S., Carouge D., Gensburger C., Anglard P., Burgun C., Dietrich J.-B., Aunis D., Zwiller J.
Mol. Pharmacol. 70:487-492(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), SUBCELLULAR LOCATION, INDUCTION BY FLUOXETINE AND COCAINE.
Strain: Wistar.
Tissue: Brain cortex.
[3]"Brain-specific phosphorylation of MeCP2 regulates activity-dependent Bdnf transcription, dendritic growth, and spine maturation."
Zhou Z., Hong E.J., Cohen S., Zhao W.-N., Ho H.H., Schmidt L., Chen W.G., Lin Y., Savner E., Griffith E.C., Hu L., Steen J.A.J., Weitz C.J., Greenberg M.E.
Neuron 52:255-269(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-80; SER-229 AND SER-421, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M94064 mRNA. Translation: AAA41584.1.
DQ897368 mRNA. Translation: ABI55237.1.
DQ897369 mRNA. Translation: ABI55238.1.
PIRA41907.
RefSeqNP_073164.2. NM_022673.2.
XP_006229628.1. XM_006229566.1.
XP_006229629.1. XM_006229567.1.
XP_006229630.1. XM_006229568.1.
UniGeneRn.38416.
Rn.9680.

3D structure databases

ProteinModelPortalQ00566.
SMRQ00566. Positions 71-195.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248038. 1 interaction.

PTM databases

PhosphoSiteQ00566.

Proteomic databases

PaxDbQ00566.
PRIDEQ00566.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29386.
KEGGrno:29386.

Organism-specific databases

CTD4204.
RGD3075. Mecp2.

Phylogenomic databases

eggNOGNOG237320.
HOGENOMHOG000015809.
HOVERGENHBG052445.
InParanoidQ00566.
KOK11588.
PhylomeDBQ00566.
TreeFamTF332974.

Gene expression databases

GenevestigatorQ00566.

Family and domain databases

Gene3D3.30.890.10. 1 hit.
InterProIPR017956. AT_hook_DNA-bd_motif.
IPR016177. DNA-bd_dom.
IPR017353. Me_CpG-bd_MeCP2.
IPR001739. Methyl_CpG_DNA-bd.
[Graphical view]
PfamPF01429. MBD. 1 hit.
[Graphical view]
PIRSFPIRSF038006. Methyl_CpG_bd_MeCP2. 1 hit.
SMARTSM00384. AT_hook. 2 hits.
SM00391. MBD. 1 hit.
[Graphical view]
SUPFAMSSF54171. SSF54171. 1 hit.
PROSITEPS50982. MBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio608987.
PROQ00566.

Entry information

Entry nameMECP2_RAT
AccessionPrimary (citable) accession number: Q00566
Secondary accession number(s): Q09HV7, Q09HV8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families