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Q00560 (IL6RB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interleukin-6 receptor subunit beta

Short name=IL-6 receptor subunit beta
Short name=IL-6R subunit beta
Short name=IL-6R-beta
Short name=IL-6RB
Alternative name(s):
Interleukin-6 signal transducer
Membrane glycoprotein 130
Short name=gp130
Oncostatin-M receptor subunit alpha
CD_antigen=CD130
Gene names
Name:Il6st
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length917 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development.

Subunit structure

Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Interacts with HCK By similarity. Forms heterodimers composed of LIPR and IL6ST (type I OSM receptor). Also forms heterodimers composed of OSMR and IL6ST (type II OSM receptor). Interacts with INPP5D/SHIP1. Ref.4 Ref.5

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver. Found in all the cell lines tested except BaF-B03. Expression not restricted to IL6-responsive cells.

Developmental stage

In embryonic stem cells it is found from day 6 of gestation. It reaches a peak on day 8 and gradually declines during the rest of embryogenesis.

Domain

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Post-translational modification

Phosphorylation of Ser-780 down-regulates cell surface expression By similarity.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 2 subfamily.

Contains 5 fibronectin type-III domains.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Il12rb2P973782EBI-3862992,EBI-6253448
Socs3O357183EBI-3862992,EBI-2659360

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 917895Interleukin-6 receptor subunit beta
PRO_0000010900

Regions

Topological domain23 – 617595Extracellular Potential
Transmembrane618 – 63922Helical; Potential
Topological domain640 – 917278Cytoplasmic Potential
Domain26 – 12095Ig-like C2-type
Domain128 – 22194Fibronectin type-III 1
Domain222 – 322101Fibronectin type-III 2
Domain327 – 41791Fibronectin type-III 3
Domain422 – 51594Fibronectin type-III 4
Domain517 – 61195Fibronectin type-III 5
Motif308 – 3125WSXWS motif
Motif649 – 6579Box 1 motif
Compositional bias723 – 74119Ser-rich

Amino acid modifications

Modified residue6651Phosphoserine By similarity
Modified residue7801Phosphoserine By similarity
Modified residue8271Phosphoserine By similarity
Glycosylation431N-linked (GlcNAc...) Potential
Glycosylation611N-linked (GlcNAc...) Potential
Glycosylation831N-linked (GlcNAc...) Potential
Glycosylation1311N-linked (GlcNAc...) Potential
Glycosylation1571N-linked (GlcNAc...) Potential
Glycosylation2251N-linked (GlcNAc...) Ref.7
Glycosylation3881N-linked (GlcNAc...) Potential
Glycosylation4761N-linked (GlcNAc...) Potential
Glycosylation5511N-linked (GlcNAc...) Potential
Disulfide bond28 ↔ 54 By similarity
Disulfide bond48 ↔ 103 By similarity
Disulfide bond134 ↔ 144 By similarity
Disulfide bond172 ↔ 180 By similarity
Disulfide bond456 ↔ 464 By similarity

Experimental info

Sequence conflict7561Q → E in CAA44515. Ref.1
Sequence conflict7561Q → E in AAA37723. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q00560 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: EDA69AB865E5A6E8

FASTA917102,451
        10         20         30         40         50         60 
MSAPRIWLAQ ALLFFLTTES IGQLLEPCGY IYPEFPVVQR GSNFTAICVL KEACLQHYYV 

        70         80         90        100        110        120 
NASYIVWKTN HAAVPREQVT VINRTTSSVT FTDVVLPSVQ LTCNILSFGQ IEQNVYGVTM 

       130        140        150        160        170        180 
LSGFPPDKPT NLTCIVNEGK NMLCQWDPGR ETYLETNYTL KSEWATEKFP DCQSKHGTSC 

       190        200        210        220        230        240 
MVSYMPTYYV NIEVWVEAEN ALGKVSSESI NFDPVDKVKP TPPYNLSVTN SEELSSILKL 

       250        260        270        280        290        300 
SWVSSGLGGL LDLKSDIQYR TKDASTWIQV PLEDTMSPRT SFTVQDLKPF TEYVFRIRSI 

       310        320        330        340        350        360 
KDSGKGYWSD WSEEASGTTY EDRPSRPPSF WYKTNPSHGQ EYRSVRLIWK ALPLSEANGK 

       370        380        390        400        410        420 
ILDYEVILTQ SKSVSQTYTV TGTELTVNLT NDRYVASLAA RNKVGKSAAA VLTIPSPHVT 

       430        440        450        460        470        480 
AAYSVVNLKA FPKDNLLWVE WTPPPKPVSK YILEWCVLSE NAPCVEDWQQ EDATVNRTHL 

       490        500        510        520        530        540 
RGRLLESKCY QITVTPVFAT GPGGSESLKA YLKQAAPARG PTVRTKKVGK NEAVLAWDQI 

       550        560        570        580        590        600 
PVDDQNGFIR NYSISYRTSV GKEMVVHVDS SHTEYTLSSL SSDTLYMVRM AAYTDEGGKD 

       610        620        630        640        650        660 
GPEFTFTTPK FAQGEIEAIV VPVCLAFLLT TLLGVLFCFN KRDLIKKHIW PNVPDPSKSH 

       670        680        690        700        710        720 
IAQWSPHTPP RHNFNSKDQM YSDGNFTDVS VVEIEANNKK PCPDDLKSVD LFKKEKVSTE 

       730        740        750        760        770        780 
GHSSGIGGSS CMSSSRPSIS SNEENESAQS TASTVQYSTV VHSGYRHQVP SVQVFSRSES 

       790        800        810        820        830        840 
TQPLLDSEER PEDLQLVDSV DGGDEILPRQ PYFKQNCSQP EACPEISHFE RSNQVLSGNE 

       850        860        870        880        890        900 
EDFVRLKQQQ VSDHISQPYG SEQRRLFQEG STADALGTGA DGQMERFESV GMETTIDEEI 

       910 
PKSYLPQTVR QGGYMPQ 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of a murine IL-6 receptor-associated signal transducer, gp130, and its regulated expression in vivo."
Saito M., Yoshida K., Hibi M., Taga T., Kishimoto T.
J. Immunol. 148:4066-4071(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
Tissue: Macrophage.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Reconstitution of the functional mouse oncostatin M (OSM) receptor: molecular cloning of the OSM receptor beta subunit."
Tanaka M., Hara T., Copeland N.G., Gilbert D.J., Jenkins N.A., Miyajima A.
Blood 93:804-815(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[5]"s-SHIP associates with receptor complexes essential for pluripotent stem cell growth and survival."
Desponts C., Ninos J.M., Kerr W.G.
Stem Cells Dev. 15:641-646(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH INPP5D.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-225.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62646 mRNA. Translation: CAA44515.1.
M83336 mRNA. Translation: AAA37723.1.
AC159196 Genomic DNA. No translation available.
CH466568 Genomic DNA. Translation: EDL18412.1.
PIRI49699.
RefSeqNP_034690.3. NM_010560.3.
UniGeneMm.4364.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2HMHX-ray2.00B753-763[»]
4GL9X-ray3.90I/J/K/L750-764[»]
ProteinModelPortalQ00560.
SMRQ00560. Positions 24-610.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-5782N.
IntActQ00560. 6 interactions.
MINTMINT-3382082.

PTM databases

PhosphoSiteQ00560.

Proteomic databases

PaxDbQ00560.
PRIDEQ00560.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000070731; ENSMUSP00000064205; ENSMUSG00000021756.
ENSMUST00000183663; ENSMUSP00000138836; ENSMUSG00000021756.
ENSMUST00000184311; ENSMUSP00000139227; ENSMUSG00000021756.
GeneID16195.
KEGGmmu:16195.
UCSCuc007rwg.2. mouse.

Organism-specific databases

CTD3572.
MGIMGI:96560. Il6st.

Phylogenomic databases

eggNOGNOG145009.
GeneTreeENSGT00550000074436.
HOGENOMHOG000015771.
HOVERGENHBG052119.
InParanoidQ00560.
KOK05060.
OMAFKQNCSQ.
OrthoDBEOG7FXZXN.
TreeFamTF338122.

Gene expression databases

ArrayExpressQ00560.
CleanExMM_IL6ST.
GenevestigatorQ00560.

Family and domain databases

Gene3D2.60.40.10. 6 hits.
InterProIPR003961. Fibronectin_type3.
IPR003529. Hematopoietin_rcpt_Gp130_CS.
IPR013783. Ig-like_fold.
IPR010457. IgC2-like_lig-bd.
IPR015321. IL-6_rcpt_alpha-bd.
[Graphical view]
PfamPF00041. fn3. 2 hits.
PF09240. IL6Ra-bind. 1 hit.
PF06328. Lep_receptor_Ig. 1 hit.
[Graphical view]
SMARTSM00060. FN3. 5 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 4 hits.
PROSITEPS50853. FN3. 5 hits.
PS01353. HEMATOPO_REC_L_F2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ00560.
NextBio289117.
PROQ00560.
SOURCESearch...

Entry information

Entry nameIL6RB_MOUSE
AccessionPrimary (citable) accession number: Q00560
Secondary accession number(s): G5E8D2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot