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Protein

Interleukin-6 receptor subunit beta

Gene

Il6st

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiREACT_277805. ERK1 activation.
REACT_301070. Interleukin-6 signaling.
REACT_353300. ERK2 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-6 receptor subunit beta
Short name:
IL-6 receptor subunit beta
Short name:
IL-6R subunit beta
Short name:
IL-6R-beta
Short name:
IL-6RB
Alternative name(s):
Interleukin-6 signal transducer
Membrane glycoprotein 130
Short name:
gp130
Oncostatin-M receptor subunit alpha
CD_antigen: CD130
Gene namesi
Name:Il6st
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:96560. Il6st.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 617595ExtracellularSequence AnalysisAdd
BLAST
Transmembranei618 – 63922HelicalSequence AnalysisAdd
BLAST
Topological domaini640 – 917278CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell body Source: MGI
  • ciliary neurotrophic factor receptor complex Source: MGI
  • dendrite Source: MGI
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • interleukin-6 receptor complex Source: MGI
  • membrane Source: MGI
  • neuronal cell body Source: MGI
  • oncostatin-M receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 917895Interleukin-6 receptor subunit betaPRO_0000010900Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 54By similarity
Glycosylationi43 – 431N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi48 ↔ 103By similarity
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi134 ↔ 144By similarity
Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi172 ↔ 180By similarity
Glycosylationi225 – 2251N-linked (GlcNAc...)1 Publication
Glycosylationi388 – 3881N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi456 ↔ 464By similarity
Glycosylationi476 – 4761N-linked (GlcNAc...)Sequence Analysis
Glycosylationi551 – 5511N-linked (GlcNAc...)Sequence Analysis
Modified residuei659 – 6591PhosphoserineBy similarity
Modified residuei665 – 6651PhosphoserineBy similarity
Modified residuei780 – 7801PhosphoserineBy similarity
Modified residuei827 – 8271PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation of Ser-780 down-regulates cell surface expression.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ00560.
PRIDEiQ00560.

PTM databases

PhosphoSiteiQ00560.

Expressioni

Tissue specificityi

Found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver. Found in all the cell lines tested except BaF-B03. Expression not restricted to IL6-responsive cells.

Developmental stagei

In embryonic stem cells it is found from day 6 of gestation. It reaches a peak on day 8 and gradually declines during the rest of embryogenesis.

Gene expression databases

CleanExiMM_IL6ST.
ExpressionAtlasiQ00560. baseline and differential.

Interactioni

Subunit structurei

Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Interacts with HCK (By similarity). Forms heterodimers composed of LIPR and IL6ST (type I OSM receptor). Also forms heterodimers composed of OSMR and IL6ST (type II OSM receptor). Interacts with INPP5D/SHIP1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Il12rb2P973782EBI-3862992,EBI-6253448
Socs3O357183EBI-3862992,EBI-2659360

Protein-protein interaction databases

BioGridi200643. 9 interactions.
DIPiDIP-5782N.
IntActiQ00560. 6 interactions.
MINTiMINT-3382082.
STRINGi10090.ENSMUSP00000064205.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BBUNMR-B750-764[»]
2HMHX-ray2.00B753-763[»]
4GL9X-ray3.90I/J/K/L750-764[»]
ProteinModelPortaliQ00560.
SMRiQ00560. Positions 24-610.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00560.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 12095Ig-like C2-typeAdd
BLAST
Domaini128 – 22194Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini222 – 322101Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini327 – 41791Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini422 – 51594Fibronectin type-III 4PROSITE-ProRule annotationAdd
BLAST
Domaini517 – 61195Fibronectin type-III 5PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi308 – 3125WSXWS motif
Motifi649 – 6579Box 1 motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi723 – 74119Ser-richAdd
BLAST

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Contains 5 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG145009.
GeneTreeiENSGT00550000074436.
HOGENOMiHOG000015771.
HOVERGENiHBG052119.
InParanoidiQ00560.
KOiK05060.
OMAiFKQNCSQ.
OrthoDBiEOG7FXZXN.
TreeFamiTF338122.

Family and domain databases

Gene3Di2.60.40.10. 6 hits.
InterProiIPR003961. FN3_dom.
IPR003529. Hematopoietin_rcpt_Gp130_CS.
IPR013783. Ig-like_fold.
IPR010457. IgC2-like_lig-bd.
IPR015321. IL-6_rcpt_alpha-bd.
[Graphical view]
PfamiPF00041. fn3. 2 hits.
PF09240. IL6Ra-bind. 1 hit.
PF06328. Lep_receptor_Ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 5 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 4 hits.
PROSITEiPS50853. FN3. 5 hits.
PS01353. HEMATOPO_REC_L_F2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPRIWLAQ ALLFFLTTES IGQLLEPCGY IYPEFPVVQR GSNFTAICVL
60 70 80 90 100
KEACLQHYYV NASYIVWKTN HAAVPREQVT VINRTTSSVT FTDVVLPSVQ
110 120 130 140 150
LTCNILSFGQ IEQNVYGVTM LSGFPPDKPT NLTCIVNEGK NMLCQWDPGR
160 170 180 190 200
ETYLETNYTL KSEWATEKFP DCQSKHGTSC MVSYMPTYYV NIEVWVEAEN
210 220 230 240 250
ALGKVSSESI NFDPVDKVKP TPPYNLSVTN SEELSSILKL SWVSSGLGGL
260 270 280 290 300
LDLKSDIQYR TKDASTWIQV PLEDTMSPRT SFTVQDLKPF TEYVFRIRSI
310 320 330 340 350
KDSGKGYWSD WSEEASGTTY EDRPSRPPSF WYKTNPSHGQ EYRSVRLIWK
360 370 380 390 400
ALPLSEANGK ILDYEVILTQ SKSVSQTYTV TGTELTVNLT NDRYVASLAA
410 420 430 440 450
RNKVGKSAAA VLTIPSPHVT AAYSVVNLKA FPKDNLLWVE WTPPPKPVSK
460 470 480 490 500
YILEWCVLSE NAPCVEDWQQ EDATVNRTHL RGRLLESKCY QITVTPVFAT
510 520 530 540 550
GPGGSESLKA YLKQAAPARG PTVRTKKVGK NEAVLAWDQI PVDDQNGFIR
560 570 580 590 600
NYSISYRTSV GKEMVVHVDS SHTEYTLSSL SSDTLYMVRM AAYTDEGGKD
610 620 630 640 650
GPEFTFTTPK FAQGEIEAIV VPVCLAFLLT TLLGVLFCFN KRDLIKKHIW
660 670 680 690 700
PNVPDPSKSH IAQWSPHTPP RHNFNSKDQM YSDGNFTDVS VVEIEANNKK
710 720 730 740 750
PCPDDLKSVD LFKKEKVSTE GHSSGIGGSS CMSSSRPSIS SNEENESAQS
760 770 780 790 800
TASTVQYSTV VHSGYRHQVP SVQVFSRSES TQPLLDSEER PEDLQLVDSV
810 820 830 840 850
DGGDEILPRQ PYFKQNCSQP EACPEISHFE RSNQVLSGNE EDFVRLKQQQ
860 870 880 890 900
VSDHISQPYG SEQRRLFQEG STADALGTGA DGQMERFESV GMETTIDEEI
910
PKSYLPQTVR QGGYMPQ
Length:917
Mass (Da):102,451
Last modified:October 3, 2012 - v2
Checksum:iEDA69AB865E5A6E8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti756 – 7561Q → E in CAA44515 (PubMed:1602143).Curated
Sequence conflicti756 – 7561Q → E in AAA37723 (PubMed:1602143).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62646 mRNA. Translation: CAA44515.1.
M83336 mRNA. Translation: AAA37723.1.
AC159196 Genomic DNA. No translation available.
CH466568 Genomic DNA. Translation: EDL18412.1.
CCDSiCCDS26773.1.
PIRiI49699.
RefSeqiNP_034690.3. NM_010560.3.
UniGeneiMm.4364.

Genome annotation databases

EnsembliENSMUST00000070731; ENSMUSP00000064205; ENSMUSG00000021756.
ENSMUST00000183663; ENSMUSP00000138836; ENSMUSG00000021756.
ENSMUST00000184311; ENSMUSP00000139227; ENSMUSG00000021756.
GeneIDi16195.
KEGGimmu:16195.
UCSCiuc007rwg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62646 mRNA. Translation: CAA44515.1.
M83336 mRNA. Translation: AAA37723.1.
AC159196 Genomic DNA. No translation available.
CH466568 Genomic DNA. Translation: EDL18412.1.
CCDSiCCDS26773.1.
PIRiI49699.
RefSeqiNP_034690.3. NM_010560.3.
UniGeneiMm.4364.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BBUNMR-B750-764[»]
2HMHX-ray2.00B753-763[»]
4GL9X-ray3.90I/J/K/L750-764[»]
ProteinModelPortaliQ00560.
SMRiQ00560. Positions 24-610.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200643. 9 interactions.
DIPiDIP-5782N.
IntActiQ00560. 6 interactions.
MINTiMINT-3382082.
STRINGi10090.ENSMUSP00000064205.

PTM databases

PhosphoSiteiQ00560.

Proteomic databases

PaxDbiQ00560.
PRIDEiQ00560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070731; ENSMUSP00000064205; ENSMUSG00000021756.
ENSMUST00000183663; ENSMUSP00000138836; ENSMUSG00000021756.
ENSMUST00000184311; ENSMUSP00000139227; ENSMUSG00000021756.
GeneIDi16195.
KEGGimmu:16195.
UCSCiuc007rwg.2. mouse.

Organism-specific databases

CTDi3572.
MGIiMGI:96560. Il6st.

Phylogenomic databases

eggNOGiNOG145009.
GeneTreeiENSGT00550000074436.
HOGENOMiHOG000015771.
HOVERGENiHBG052119.
InParanoidiQ00560.
KOiK05060.
OMAiFKQNCSQ.
OrthoDBiEOG7FXZXN.
TreeFamiTF338122.

Enzyme and pathway databases

ReactomeiREACT_277805. ERK1 activation.
REACT_301070. Interleukin-6 signaling.
REACT_353300. ERK2 activation.

Miscellaneous databases

EvolutionaryTraceiQ00560.
NextBioi289117.
PROiQ00560.
SOURCEiSearch...

Gene expression databases

CleanExiMM_IL6ST.
ExpressionAtlasiQ00560. baseline and differential.

Family and domain databases

Gene3Di2.60.40.10. 6 hits.
InterProiIPR003961. FN3_dom.
IPR003529. Hematopoietin_rcpt_Gp130_CS.
IPR013783. Ig-like_fold.
IPR010457. IgC2-like_lig-bd.
IPR015321. IL-6_rcpt_alpha-bd.
[Graphical view]
PfamiPF00041. fn3. 2 hits.
PF09240. IL6Ra-bind. 1 hit.
PF06328. Lep_receptor_Ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 5 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 4 hits.
PROSITEiPS50853. FN3. 5 hits.
PS01353. HEMATOPO_REC_L_F2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a murine IL-6 receptor-associated signal transducer, gp130, and its regulated expression in vivo."
    Saito M., Yoshida K., Hibi M., Taga T., Kishimoto T.
    J. Immunol. 148:4066-4071(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
    Tissue: Macrophage.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Reconstitution of the functional mouse oncostatin M (OSM) receptor: molecular cloning of the OSM receptor beta subunit."
    Tanaka M., Hara T., Copeland N.G., Gilbert D.J., Jenkins N.A., Miyajima A.
    Blood 93:804-815(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  5. "s-SHIP associates with receptor complexes essential for pluripotent stem cell growth and survival."
    Desponts C., Ninos J.M., Kerr W.G.
    Stem Cells Dev. 15:641-646(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH INPP5D.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-225.

Entry informationi

Entry nameiIL6RB_MOUSE
AccessioniPrimary (citable) accession number: Q00560
Secondary accession number(s): G5E8D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 3, 2012
Last modified: June 24, 2015
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.