Q00560 (IL6RB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-6 receptor subunit beta
Short name=IL-6 receptor subunit beta
Short name=IL-6R subunit beta
Short name=IL-6R-beta
Short name=IL-6RB

Alternative name(s):
Interleukin-6 signal transducer
Membrane glycoprotein 130
Short name=gp130
Oncostatin-M receptor subunit alpha
CD_antigen=CD130

Gene names

Name:

Il6st

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	917 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development.
Subunit structure	Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Interacts with HCK By similarity. Forms heterodimers composed of LIPR and IL6ST (type I OSM receptor). Also forms heterodimers composed of OSMR and IL6ST (type II OSM receptor). Interacts with INPP5D/SHIP1. Ref.4 Ref.5
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver. Found in all the cell lines tested except BaF-B03. Expression not restricted to IL6-responsive cells.
Developmental stage	In embryonic stem cells it is found from day 6 of gestation. It reaches a peak on day 8 and gradually declines during the rest of embryogenesis.
Domain	The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
Post-translational modification	Phosphorylation of Ser-780 down-regulates cell surface expression By similarity.
Sequence similarities	Belongs to the type I cytokine receptor family. Type 2 subfamily. Contains 5 fibronectin type-III domains. Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen metabolic process Inferred from mutant phenotype PubMed 10205167. Source: MGI interleukin-11-mediated signaling pathway Inferred from direct assay PubMed 7957045. Source: GOC interleukin-27-mediated signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of interleukin-6-mediated signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of T cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of astrocyte differentiation Inferred from mutant phenotype PubMed 10486560. Source: MGI positive regulation of cell proliferation Inferred from genetic interaction PubMed 15051883. Source: MGI positive regulation of osteoblast differentiation Inferred from electronic annotation. Source: Compara positive regulation of tyrosine phosphorylation of Stat1 protein Inferred from electronic annotation. Source: Compara positive regulation of tyrosine phosphorylation of Stat3 protein Inferred from electronic annotation. Source: Compara regulation of Notch signaling pathway Inferred from direct assay PubMed 12629177. Source: MGI
Cellular_component	ciliary neurotrophic factor receptor complex Inferred from electronic annotation. Source: Compara dendrite Inferred from direct assay PubMed 10989258. Source: MGI external side of plasma membrane Inferred from direct assay PubMed 11275690. Source: MGI extracellular space Inferred from electronic annotation. Source: Compara interleukin-6 receptor complex Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from direct assay PubMed 10989258. Source: MGI oncostatin-M receptor complex Inferred from electronic annotation. Source: Compara
Molecular_function	interleukin-6 receptor activity Inferred from electronic annotation. Source: Compara leukemia inhibitory factor receptor activity Inferred from electronic annotation. Source: Compara oncostatin-M receptor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Il12rb2	P97378	2	EBI-3862992,EBI-6253448

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 917	895	Interleukin-6 receptor subunit beta		PRO_0000010900
Regions
	Topological domain	23 – 617	595	Extracellular Potential
	Transmembrane	618 – 639	22	Helical; Potential
	Topological domain	640 – 917	278	Cytoplasmic Potential
	Domain	26 – 120	95	Ig-like C2-type
	Domain	125 – 214	90	Fibronectin type-III 1
	Domain	220 – 319	100	Fibronectin type-III 2
	Domain	324 – 416	93	Fibronectin type-III 3
	Domain	421 – 512	92	Fibronectin type-III 4
	Domain	514 – 608	95	Fibronectin type-III 5
	Motif	308 – 312	5	WSXWS motif
	Motif	649 – 657	9	Box 1 motif
	Compositional bias	723 – 741	19	Ser-rich
Amino acid modifications
	Modified residue	665	1	Phosphoserine By similarity
	Modified residue	780	1	Phosphoserine By similarity
	Modified residue	827	1	Phosphoserine By similarity
	Glycosylation	43	1	N-linked (GlcNAc...) Potential
	Glycosylation	61	1	N-linked (GlcNAc...) Potential
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Glycosylation	157	1	N-linked (GlcNAc...) Potential
	Glycosylation	225	1	N-linked (GlcNAc...) Ref.6
	Glycosylation	388	1	N-linked (GlcNAc...) Potential
	Glycosylation	476	1	N-linked (GlcNAc...) Potential
	Glycosylation	551	1	N-linked (GlcNAc...) Potential
	Disulfide bond	28 ↔ 54		By similarity
	Disulfide bond	48 ↔ 103		By similarity
	Disulfide bond	134 ↔ 144		By similarity
	Disulfide bond	172 ↔ 180		By similarity
	Disulfide bond	456 ↔ 464		By similarity
Experimental info
	Sequence conflict	756	1	Q → E in CAA44515. Ref.1
	Sequence conflict	756	1	Q → E in AAA37723. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q00560 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: EDA69AB865E5A6E8	FASTA	917	102,451
	10 20 30 40 50 60 MSAPRIWLAQ ALLFFLTTES IGQLLEPCGY IYPEFPVVQR GSNFTAICVL KEACLQHYYV 70 80 90 100 110 120 NASYIVWKTN HAAVPREQVT VINRTTSSVT FTDVVLPSVQ LTCNILSFGQ IEQNVYGVTM 130 140 150 160 170 180 LSGFPPDKPT NLTCIVNEGK NMLCQWDPGR ETYLETNYTL KSEWATEKFP DCQSKHGTSC 190 200 210 220 230 240 MVSYMPTYYV NIEVWVEAEN ALGKVSSESI NFDPVDKVKP TPPYNLSVTN SEELSSILKL 250 260 270 280 290 300 SWVSSGLGGL LDLKSDIQYR TKDASTWIQV PLEDTMSPRT SFTVQDLKPF TEYVFRIRSI 310 320 330 340 350 360 KDSGKGYWSD WSEEASGTTY EDRPSRPPSF WYKTNPSHGQ EYRSVRLIWK ALPLSEANGK 370 380 390 400 410 420 ILDYEVILTQ SKSVSQTYTV TGTELTVNLT NDRYVASLAA RNKVGKSAAA VLTIPSPHVT 430 440 450 460 470 480 AAYSVVNLKA FPKDNLLWVE WTPPPKPVSK YILEWCVLSE NAPCVEDWQQ EDATVNRTHL 490 500 510 520 530 540 RGRLLESKCY QITVTPVFAT GPGGSESLKA YLKQAAPARG PTVRTKKVGK NEAVLAWDQI 550 560 570 580 590 600 PVDDQNGFIR NYSISYRTSV GKEMVVHVDS SHTEYTLSSL SSDTLYMVRM AAYTDEGGKD 610 620 630 640 650 660 GPEFTFTTPK FAQGEIEAIV VPVCLAFLLT TLLGVLFCFN KRDLIKKHIW PNVPDPSKSH 670 680 690 700 710 720 IAQWSPHTPP RHNFNSKDQM YSDGNFTDVS VVEIEANNKK PCPDDLKSVD LFKKEKVSTE 730 740 750 760 770 780 GHSSGIGGSS CMSSSRPSIS SNEENESAQS TASTVQYSTV VHSGYRHQVP SVQVFSRSES 790 800 810 820 830 840 TQPLLDSEER PEDLQLVDSV DGGDEILPRQ PYFKQNCSQP EACPEISHFE RSNQVLSGNE 850 860 870 880 890 900 EDFVRLKQQQ VSDHISQPYG SEQRRLFQEG STADALGTGA DGQMERFESV GMETTIDEEI 910 PKSYLPQTVR QGGYMPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a murine IL-6 receptor-associated signal transducer, gp130, and its regulated expression in vivo." Saito M., Yoshida K., Hibi M., Taga T., Kishimoto T. J. Immunol. 148:4066-4071(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR. Tissue: Macrophage.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Reconstitution of the functional mouse oncostatin M (OSM) receptor: molecular cloning of the OSM receptor beta subunit." Tanaka M., Hara T., Copeland N.G., Gilbert D.J., Jenkins N.A., Miyajima A. Blood 93:804-815(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
[5]	"s-SHIP associates with receptor complexes essential for pluripotent stem cell growth and survival." Desponts C., Ninos J.M., Kerr W.G. Stem Cells Dev. 15:641-646(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH INPP5D.
[6]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-225, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X62646 mRNA. Translation: CAA44515.1.
M83336 mRNA. Translation: AAA37723.1.
AC159196 Genomic DNA. No translation available.
CH466568 Genomic DNA. Translation: EDL18412.1.

IPI

IPI00120155.

PIR

I49699.

RefSeq

NP_034690.3. NM_010560.3.

UniGene

Mm.4364.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HMH	X-ray	2.00	B	753-763	[»]
4GL9	X-ray	3.90	I/J/K/L	750-764	[»]

ProteinModelPortal

Q00560.

SMR

Q00560. Positions 24-610.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5782N.

IntAct

Q00560. 4 interactions.

MINT

MINT-3382082.

PTM databases

PhosphoSite

Q00560.

Proteomic databases

PaxDb

Q00560.

PRIDE

Q00560.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000070731; ENSMUSP00000064205; ENSMUSG00000021756.

GeneID

16195.

KEGG

mmu:16195.

Organism-specific databases

CTD

3572.

MGI

MGI:96560. Il6st.

Phylogenomic databases

eggNOG

NOG145009.

GeneTree

ENSGT00550000074436.

HOGENOM

HOG000015771.

HOVERGEN

HBG052119.

InParanoid

Q00560.

KO

K05060.

OMA

KDQMYSD.

OrthoDB

EOG42BX7V.

Gene expression databases

CleanEx

MM_IL6ST.

Genevestigator

Q00560.

GermOnline

ENSMUSG00000021756. Mus musculus.

Family and domain databases

Gene3D

2.60.40.10. 6 hits.

InterPro

IPR003961. Fibronectin_type3.
IPR003529. Hematopoietin_rcpt_Gp130_CS.
IPR013783. Ig-like_fold.
IPR010457. IgC2-like_lig-bd.
IPR015321. IL6_recept-bd.
[Graphical view]

Pfam

PF00041. fn3. 2 hits.
PF09240. IL6Ra-bind. 1 hit.
PF06328. Lep_receptor_Ig. 1 hit.
[Graphical view]

SMART

SM00060. FN3. 5 hits.
[Graphical view]

SUPFAM

SSF49265. FN_III-like. 6 hits.

PROSITE

PS50853. FN3. 5 hits.
PS01353. HEMATOPO_REC_L_F2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q00560.

NextBio

289117.

SOURCE

Search...

Entry information

Entry name

IL6RB_MOUSE

Accession

Primary (citable) accession number: Q00560
Secondary accession number(s): G5E8D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program