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Q00560

- IL6RB_MOUSE

UniProt

Q00560 - IL6RB_MOUSE

Protein

Interleukin-6 receptor subunit beta

Gene

Il6st

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development.

    GO - Molecular functioni

    1. cytokine receptor activity Source: InterPro
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cytokine-mediated signaling pathway Source: MGI
    2. glycogen metabolic process Source: MGI
    3. interleukin-11-mediated signaling pathway Source: GOC
    4. positive regulation of astrocyte differentiation Source: MGI
    5. positive regulation of cell proliferation Source: MGI
    6. regulation of Notch signaling pathway Source: MGI
    7. signal transduction Source: MGI

    Keywords - Molecular functioni

    Receptor

    Enzyme and pathway databases

    ReactomeiREACT_199118. Interleukin-6 signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interleukin-6 receptor subunit beta
    Short name:
    IL-6 receptor subunit beta
    Short name:
    IL-6R subunit beta
    Short name:
    IL-6R-beta
    Short name:
    IL-6RB
    Alternative name(s):
    Interleukin-6 signal transducer
    Membrane glycoprotein 130
    Short name:
    gp130
    Oncostatin-M receptor subunit alpha
    CD_antigen: CD130
    Gene namesi
    Name:Il6st
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:96560. Il6st.

    Subcellular locationi

    GO - Cellular componenti

    1. cell body Source: MGI
    2. dendrite Source: MGI
    3. external side of plasma membrane Source: MGI
    4. integral component of membrane Source: UniProtKB-KW
    5. neuronal cell body Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 917895Interleukin-6 receptor subunit betaPRO_0000010900Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi28 ↔ 54By similarity
    Glycosylationi43 – 431N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi48 ↔ 103By similarity
    Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi134 ↔ 144By similarity
    Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi172 ↔ 180By similarity
    Glycosylationi225 – 2251N-linked (GlcNAc...)1 Publication
    Glycosylationi388 – 3881N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi456 ↔ 464By similarity
    Glycosylationi476 – 4761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi551 – 5511N-linked (GlcNAc...)Sequence Analysis
    Modified residuei665 – 6651PhosphoserineBy similarity
    Modified residuei780 – 7801PhosphoserineBy similarity
    Modified residuei827 – 8271PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation of Ser-780 down-regulates cell surface expression.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ00560.
    PRIDEiQ00560.

    PTM databases

    PhosphoSiteiQ00560.

    Expressioni

    Tissue specificityi

    Found in tissues such as brain, heart, thymus, spleen, kidney, lung and liver. Found in all the cell lines tested except BaF-B03. Expression not restricted to IL6-responsive cells.

    Developmental stagei

    In embryonic stem cells it is found from day 6 of gestation. It reaches a peak on day 8 and gradually declines during the rest of embryogenesis.

    Gene expression databases

    ArrayExpressiQ00560.
    CleanExiMM_IL6ST.
    GenevestigatoriQ00560.

    Interactioni

    Subunit structurei

    Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Interacts with HCK By similarity. Forms heterodimers composed of LIPR and IL6ST (type I OSM receptor). Also forms heterodimers composed of OSMR and IL6ST (type II OSM receptor). Interacts with INPP5D/SHIP1.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Il12rb2P973782EBI-3862992,EBI-6253448
    Socs3O357183EBI-3862992,EBI-2659360

    Protein-protein interaction databases

    DIPiDIP-5782N.
    IntActiQ00560. 6 interactions.
    MINTiMINT-3382082.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2BBUNMR-B750-764[»]
    2HMHX-ray2.00B753-763[»]
    4GL9X-ray3.90I/J/K/L750-764[»]
    ProteinModelPortaliQ00560.
    SMRiQ00560. Positions 24-610.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ00560.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 617595ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini640 – 917278CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei618 – 63922HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 12095Ig-like C2-typeAdd
    BLAST
    Domaini128 – 22194Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini222 – 322101Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini327 – 41791Fibronectin type-III 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini422 – 51594Fibronectin type-III 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini517 – 61195Fibronectin type-III 5PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi308 – 3125WSXWS motif
    Motifi649 – 6579Box 1 motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi723 – 74119Ser-richAdd
    BLAST

    Domaini

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

    Sequence similaritiesi

    Contains 5 fibronectin type-III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG145009.
    GeneTreeiENSGT00550000074436.
    HOGENOMiHOG000015771.
    HOVERGENiHBG052119.
    InParanoidiQ00560.
    KOiK05060.
    OMAiFKQNCSQ.
    OrthoDBiEOG7FXZXN.
    TreeFamiTF338122.

    Family and domain databases

    Gene3Di2.60.40.10. 6 hits.
    InterProiIPR003961. Fibronectin_type3.
    IPR003529. Hematopoietin_rcpt_Gp130_CS.
    IPR013783. Ig-like_fold.
    IPR010457. IgC2-like_lig-bd.
    IPR015321. IL-6_rcpt_alpha-bd.
    [Graphical view]
    PfamiPF00041. fn3. 2 hits.
    PF09240. IL6Ra-bind. 1 hit.
    PF06328. Lep_receptor_Ig. 1 hit.
    [Graphical view]
    SMARTiSM00060. FN3. 5 hits.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 4 hits.
    PROSITEiPS50853. FN3. 5 hits.
    PS01353. HEMATOPO_REC_L_F2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00560-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAPRIWLAQ ALLFFLTTES IGQLLEPCGY IYPEFPVVQR GSNFTAICVL    50
    KEACLQHYYV NASYIVWKTN HAAVPREQVT VINRTTSSVT FTDVVLPSVQ 100
    LTCNILSFGQ IEQNVYGVTM LSGFPPDKPT NLTCIVNEGK NMLCQWDPGR 150
    ETYLETNYTL KSEWATEKFP DCQSKHGTSC MVSYMPTYYV NIEVWVEAEN 200
    ALGKVSSESI NFDPVDKVKP TPPYNLSVTN SEELSSILKL SWVSSGLGGL 250
    LDLKSDIQYR TKDASTWIQV PLEDTMSPRT SFTVQDLKPF TEYVFRIRSI 300
    KDSGKGYWSD WSEEASGTTY EDRPSRPPSF WYKTNPSHGQ EYRSVRLIWK 350
    ALPLSEANGK ILDYEVILTQ SKSVSQTYTV TGTELTVNLT NDRYVASLAA 400
    RNKVGKSAAA VLTIPSPHVT AAYSVVNLKA FPKDNLLWVE WTPPPKPVSK 450
    YILEWCVLSE NAPCVEDWQQ EDATVNRTHL RGRLLESKCY QITVTPVFAT 500
    GPGGSESLKA YLKQAAPARG PTVRTKKVGK NEAVLAWDQI PVDDQNGFIR 550
    NYSISYRTSV GKEMVVHVDS SHTEYTLSSL SSDTLYMVRM AAYTDEGGKD 600
    GPEFTFTTPK FAQGEIEAIV VPVCLAFLLT TLLGVLFCFN KRDLIKKHIW 650
    PNVPDPSKSH IAQWSPHTPP RHNFNSKDQM YSDGNFTDVS VVEIEANNKK 700
    PCPDDLKSVD LFKKEKVSTE GHSSGIGGSS CMSSSRPSIS SNEENESAQS 750
    TASTVQYSTV VHSGYRHQVP SVQVFSRSES TQPLLDSEER PEDLQLVDSV 800
    DGGDEILPRQ PYFKQNCSQP EACPEISHFE RSNQVLSGNE EDFVRLKQQQ 850
    VSDHISQPYG SEQRRLFQEG STADALGTGA DGQMERFESV GMETTIDEEI 900
    PKSYLPQTVR QGGYMPQ 917
    Length:917
    Mass (Da):102,451
    Last modified:October 3, 2012 - v2
    Checksum:iEDA69AB865E5A6E8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti756 – 7561Q → E in CAA44515. (PubMed:1602143)Curated
    Sequence conflicti756 – 7561Q → E in AAA37723. (PubMed:1602143)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62646 mRNA. Translation: CAA44515.1.
    M83336 mRNA. Translation: AAA37723.1.
    AC159196 Genomic DNA. No translation available.
    CH466568 Genomic DNA. Translation: EDL18412.1.
    CCDSiCCDS26773.1.
    PIRiI49699.
    RefSeqiNP_034690.3. NM_010560.3.
    UniGeneiMm.4364.

    Genome annotation databases

    EnsembliENSMUST00000070731; ENSMUSP00000064205; ENSMUSG00000021756.
    ENSMUST00000183663; ENSMUSP00000138836; ENSMUSG00000021756.
    ENSMUST00000184311; ENSMUSP00000139227; ENSMUSG00000021756.
    GeneIDi16195.
    KEGGimmu:16195.
    UCSCiuc007rwg.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62646 mRNA. Translation: CAA44515.1 .
    M83336 mRNA. Translation: AAA37723.1 .
    AC159196 Genomic DNA. No translation available.
    CH466568 Genomic DNA. Translation: EDL18412.1 .
    CCDSi CCDS26773.1.
    PIRi I49699.
    RefSeqi NP_034690.3. NM_010560.3.
    UniGenei Mm.4364.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2BBU NMR - B 750-764 [» ]
    2HMH X-ray 2.00 B 753-763 [» ]
    4GL9 X-ray 3.90 I/J/K/L 750-764 [» ]
    ProteinModelPortali Q00560.
    SMRi Q00560. Positions 24-610.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-5782N.
    IntActi Q00560. 6 interactions.
    MINTi MINT-3382082.

    PTM databases

    PhosphoSitei Q00560.

    Proteomic databases

    PaxDbi Q00560.
    PRIDEi Q00560.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000070731 ; ENSMUSP00000064205 ; ENSMUSG00000021756 .
    ENSMUST00000183663 ; ENSMUSP00000138836 ; ENSMUSG00000021756 .
    ENSMUST00000184311 ; ENSMUSP00000139227 ; ENSMUSG00000021756 .
    GeneIDi 16195.
    KEGGi mmu:16195.
    UCSCi uc007rwg.2. mouse.

    Organism-specific databases

    CTDi 3572.
    MGIi MGI:96560. Il6st.

    Phylogenomic databases

    eggNOGi NOG145009.
    GeneTreei ENSGT00550000074436.
    HOGENOMi HOG000015771.
    HOVERGENi HBG052119.
    InParanoidi Q00560.
    KOi K05060.
    OMAi FKQNCSQ.
    OrthoDBi EOG7FXZXN.
    TreeFami TF338122.

    Enzyme and pathway databases

    Reactomei REACT_199118. Interleukin-6 signaling.

    Miscellaneous databases

    EvolutionaryTracei Q00560.
    NextBioi 289117.
    PROi Q00560.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q00560.
    CleanExi MM_IL6ST.
    Genevestigatori Q00560.

    Family and domain databases

    Gene3Di 2.60.40.10. 6 hits.
    InterProi IPR003961. Fibronectin_type3.
    IPR003529. Hematopoietin_rcpt_Gp130_CS.
    IPR013783. Ig-like_fold.
    IPR010457. IgC2-like_lig-bd.
    IPR015321. IL-6_rcpt_alpha-bd.
    [Graphical view ]
    Pfami PF00041. fn3. 2 hits.
    PF09240. IL6Ra-bind. 1 hit.
    PF06328. Lep_receptor_Ig. 1 hit.
    [Graphical view ]
    SMARTi SM00060. FN3. 5 hits.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 4 hits.
    PROSITEi PS50853. FN3. 5 hits.
    PS01353. HEMATOPO_REC_L_F2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a murine IL-6 receptor-associated signal transducer, gp130, and its regulated expression in vivo."
      Saito M., Yoshida K., Hibi M., Taga T., Kishimoto T.
      J. Immunol. 148:4066-4071(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR.
      Tissue: Macrophage.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Reconstitution of the functional mouse oncostatin M (OSM) receptor: molecular cloning of the OSM receptor beta subunit."
      Tanaka M., Hara T., Copeland N.G., Gilbert D.J., Jenkins N.A., Miyajima A.
      Blood 93:804-815(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    5. "s-SHIP associates with receptor complexes essential for pluripotent stem cell growth and survival."
      Desponts C., Ninos J.M., Kerr W.G.
      Stem Cells Dev. 15:641-646(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH INPP5D.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-225.

    Entry informationi

    Entry nameiIL6RB_MOUSE
    AccessioniPrimary (citable) accession number: Q00560
    Secondary accession number(s): G5E8D2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3