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Protein

Cytochrome P450 1A1

Gene

CYP1A1

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei228 – 2281SubstrateBy similarity
Metal bindingi461 – 4611Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A1 (EC:1.14.14.1)
Alternative name(s):
CYPIA1
Cytochrome P-450MC
Gene namesi
Name:CYP1A1
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Cytochrome P450 1A1PRO_0000051630Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711O-linked (GlcNAc)By similarity

Keywords - PTMi

Glycoprotein

Expressioni

Inductioni

By 3-methylcholanthrene (3MC).

Structurei

3D structure databases

ProteinModelPortaliQ00557.
SMRiQ00557. Positions 36-513.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG106944.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00557-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSIYGLLNF MSATELLLAI TVFCLGFWVV RALRTQVPKG LKTPPGPWGL
60 70 80 90 100
PILGHVLTLG KNPHLSLTKL SKQYGDVLQI RIGSTPVVVL SGLDTIRQAL
110 120 130 140 150
VRQGDDFKGR PDFYSFTLIT NGKSMTFNPD CGPVWAARRR LAQDALKSFS
160 170 180 190 200
IALDPASASS CYLEEYVIKE ADYLISKFQK LMAEVGHFDP DRYLVVSVTN
210 220 230 240 250
VICAMCFGQR YDHDDQELLS IVNLSNEFGK VTGSGYPPDF IPILRYLPNS
260 270 280 290 300
SLDAFKDLNK KFYSFMQKSV KEHYRTFEKG HIRDITDSLI EHCQDKSLDE
310 320 330 340 350
NANVQLSDDR VINIIVDLFG AGFDTVTTAI SWSLMYLVTN PGVQRKIQEE
360 370 380 390 400
LDTVIGRSRR PRLCDRSQLP YLEAFILETF RHSSFLPFTI PHSTTRDTSL
410 420 430 440 450
CGFYIPKGHC VFVNQWQINH NQELWGDPNK FRPERFLTSS GTLDKVLSGK
460 470 480 490 500
VTLFGLGKRK CIGETIGRLE VFLFLAILLQ QIEFTVSPGE KVDMTPIYGL
510 520
TLKHARCEYF QAQTRSSGPQ HPQA
Length:524
Mass (Da):59,136
Last modified:December 15, 1998 - v2
Checksum:i2A4C6AF9AE5FD8E0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181L → V in BAA01096. (PubMed:1778988)Curated
Sequence conflicti521 – 5211H → Y in BAA01096. (PubMed:1778988)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10251 mRNA. Translation: BAA01096.1.
D12977 mRNA. Translation: BAA02354.1.
D10913 Genomic DNA. Translation: BAA01717.1.
PIRiJX0189.
RefSeqiNP_001268352.1. NM_001281423.1.

Genome annotation databases

GeneIDi101844540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10251 mRNA. Translation: BAA01096.1.
D12977 mRNA. Translation: BAA02354.1.
D10913 Genomic DNA. Translation: BAA01717.1.
PIRiJX0189.
RefSeqiNP_001268352.1. NM_001281423.1.

3D structure databases

ProteinModelPortaliQ00557.
SMRiQ00557. Positions 36-513.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101844540.

Phylogenomic databases

HOVERGENiHBG106944.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Hamster cytochrome P-450 IA gene family, P-450IA1 and P-450IA2 in lung and liver: cDNA cloning and sequence analysis."
    Sagami I., Ohmachi T., Fujii H., Kikuchi H., Watanabe M.
    J. Biochem. 110:641-647(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "Molecular cloning and expression of hamster cytochrome P450 1A1 and cytochrome P450 reductase."
    Ohgiya S., Goda T., Ishizaki K., Morimoto M., Sakamoto T., Kamataki T., Shinriki N.
    Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiCP1A1_MESAU
AccessioniPrimary (citable) accession number: Q00557
Secondary accession number(s): Q64665
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 15, 1998
Last modified: January 7, 2015
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.