Reviewed, UniProtKB/Swiss-Prot Q00557 (CP1A1_MESAU)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 1A1
    EC=1.14.14.1
Alternative name(s):
    CYPIA1
    Cytochrome P-450MC

Gene names

Name:

CYP1A1

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By 3-methylcholanthrene (3MC).
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Cytochrome P450 1A1		PRO_0000051630
Sites
	Metal binding	461	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	18	1	L → V in BAA01096. Ref.1
	Sequence conflict	521	1	H → Y in BAA01096. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q00557-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 2A4C6AF9AE5FD8E0	FASTA	524	59,136
	10 20 30 40 50 60 MSSIYGLLNF MSATELLLAI TVFCLGFWVV RALRTQVPKG LKTPPGPWGL PILGHVLTLG 70 80 90 100 110 120 KNPHLSLTKL SKQYGDVLQI RIGSTPVVVL SGLDTIRQAL VRQGDDFKGR PDFYSFTLIT 130 140 150 160 170 180 NGKSMTFNPD CGPVWAARRR LAQDALKSFS IALDPASASS CYLEEYVIKE ADYLISKFQK 190 200 210 220 230 240 LMAEVGHFDP DRYLVVSVTN VICAMCFGQR YDHDDQELLS IVNLSNEFGK VTGSGYPPDF 250 260 270 280 290 300 IPILRYLPNS SLDAFKDLNK KFYSFMQKSV KEHYRTFEKG HIRDITDSLI EHCQDKSLDE 310 320 330 340 350 360 NANVQLSDDR VINIIVDLFG AGFDTVTTAI SWSLMYLVTN PGVQRKIQEE LDTVIGRSRR 370 380 390 400 410 420 PRLCDRSQLP YLEAFILETF RHSSFLPFTI PHSTTRDTSL CGFYIPKGHC VFVNQWQINH 430 440 450 460 470 480 NQELWGDPNK FRPERFLTSS GTLDKVLSGK VTLFGLGKRK CIGETIGRLE VFLFLAILLQ 490 500 510 520 QIEFTVSPGE KVDMTPIYGL TLKHARCEYF QAQTRSSGPQ HPQA

References

[1]	"Hamster cytochrome P-450 IA gene family, P-450IA1 and P-450IA2 in lung and liver: cDNA cloning and sequence analysis." Sagami I., Ohmachi T., Fujii H., Kikuchi H., Watanabe M. J. Biochem. 110:641-647(1991) [PubMed: 1778988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"Molecular cloning and expression of hamster cytochrome P450 1A1 and cytochrome P450 reductase." Ohgiya S., Goda T., Ishizaki K., Morimoto M., Sakamoto T., Kamataki T., Shinriki N. Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10251 mRNA. Translation: BAA01096.1. D12977 mRNA. Translation: BAA02354.1. D10913 Genomic DNA. Translation: BAA01717.1.
PIR	JX0189.
3D structure databases
SMR	Q00557. Positions 36-513.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q00557.
Enzyme and pathway databases
BRENDA	1.14.14.1. 824.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit. PTHR19383:SF63. Cyt_P450_E_grp-I_CYP1. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP1A1_MESAU

Accession

Primary (citable) accession number: Q00557
Secondary accession number(s): Q64665

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	December 15, 1998
Last modified:	January 19, 2010
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)