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Q00557 (CP1A1_MESAU) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 1A1

EC=1.14.14.1
Alternative name(s):
CYPIA1
Cytochrome P-450MC
Gene names
Name:CYP1A1
OrganismMesocricetus auratus (Golden hamster)
Taxonomic identifier10036 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

By 3-methylcholanthrene (3MC).

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaromatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 524524Cytochrome P450 1A1
PRO_0000051630

Sites

Metal binding4611Iron (heme axial ligand) By similarity
Binding site2281Substrate By similarity

Experimental info

Sequence conflict181L → V in BAA01096. Ref.1
Sequence conflict5211H → Y in BAA01096. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q00557 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 2A4C6AF9AE5FD8E0

FASTA52459,136
        10         20         30         40         50         60 
MSSIYGLLNF MSATELLLAI TVFCLGFWVV RALRTQVPKG LKTPPGPWGL PILGHVLTLG 

        70         80         90        100        110        120 
KNPHLSLTKL SKQYGDVLQI RIGSTPVVVL SGLDTIRQAL VRQGDDFKGR PDFYSFTLIT 

       130        140        150        160        170        180 
NGKSMTFNPD CGPVWAARRR LAQDALKSFS IALDPASASS CYLEEYVIKE ADYLISKFQK 

       190        200        210        220        230        240 
LMAEVGHFDP DRYLVVSVTN VICAMCFGQR YDHDDQELLS IVNLSNEFGK VTGSGYPPDF 

       250        260        270        280        290        300 
IPILRYLPNS SLDAFKDLNK KFYSFMQKSV KEHYRTFEKG HIRDITDSLI EHCQDKSLDE 

       310        320        330        340        350        360 
NANVQLSDDR VINIIVDLFG AGFDTVTTAI SWSLMYLVTN PGVQRKIQEE LDTVIGRSRR 

       370        380        390        400        410        420 
PRLCDRSQLP YLEAFILETF RHSSFLPFTI PHSTTRDTSL CGFYIPKGHC VFVNQWQINH 

       430        440        450        460        470        480 
NQELWGDPNK FRPERFLTSS GTLDKVLSGK VTLFGLGKRK CIGETIGRLE VFLFLAILLQ 

       490        500        510        520 
QIEFTVSPGE KVDMTPIYGL TLKHARCEYF QAQTRSSGPQ HPQA 

« Hide

References

[1]"Hamster cytochrome P-450 IA gene family, P-450IA1 and P-450IA2 in lung and liver: cDNA cloning and sequence analysis."
Sagami I., Ohmachi T., Fujii H., Kikuchi H., Watanabe M.
J. Biochem. 110:641-647(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]"Molecular cloning and expression of hamster cytochrome P450 1A1 and cytochrome P450 reductase."
Ohgiya S., Goda T., Ishizaki K., Morimoto M., Sakamoto T., Kamataki T., Shinriki N.
Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10251 mRNA. Translation: BAA01096.1.
D12977 mRNA. Translation: BAA02354.1.
D10913 Genomic DNA. Translation: BAA01717.1.
PIRJX0189.
RefSeqNP_001268352.1. NM_001281423.1.

3D structure databases

ProteinModelPortalQ00557.
SMRQ00557. Positions 36-513.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101844540.

Phylogenomic databases

HOVERGENHBG106944.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP1A1_MESAU
AccessionPrimary (citable) accession number: Q00557
Secondary accession number(s): Q64665
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 15, 1998
Last modified: December 11, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families