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Q00539 (NAM8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein NAM8
Gene names
Name:NAM8
Synonyms:MRE2
Ordered Locus Names:YHR086W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length523 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a suppressor of mitochondrial splicing deficiencies when overexpressed. Could be a non-essential component of the mitochondrial splicing machinery.

Subcellular location

Mitochondrion.

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.

Sequence similarities

Contains 3 RRM (RNA recognition motif) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 523523Protein NAM8
PRO_0000081659

Regions

Domain54 – 14592RRM 1
Domain163 – 24280RRM 2
Domain313 – 38573RRM 3

Experimental info

Sequence conflict1801F → L in CAA46011. Ref.1
Sequence conflict208 – 2092GF → VL in CAA46011. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q00539 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 64F198EEFB32A909

FASTA52356,972
        10         20         30         40         50         60 
MSYKQTTYYP SRGNLVRNDS SPYTNTISSE TNNSSTSVLS LQGASNVSLG TTGNQLYMGD 

        70         80         90        100        110        120 
LDPTWDKNTV RQIWASLGEA NINVRMMWNN TLNNGSRSSM GPKNNQGYCF VDFPSSTHAA 

       130        140        150        160        170        180 
NALLKNGMLI PNFPNKKLKL NWATSSYSNS NNSLNNVKSG NNCSIFVGDL APNVTESQLF 

       190        200        210        220        230        240 
ELFINRYAST SHAKIVHDQV TGMSKGYGFV KFTNSDEQQL ALSEMQGVFL NGRAIKVGPT 

       250        260        270        280        290        300 
SGQQQHVSGN NDYNRSSSSL NNENVDSRFL SKGQSFLSNG NNNMGFKRNH MSQFIYPVQQ 

       310        320        330        340        350        360 
QPSLNHFTDP NNTTVFIGGL SSLVTEDELR AYFQPFGTIV YVKIPVGKCC GFVQYVDRLS 

       370        380        390        400        410        420 
AEAAIAGMQG FPIANSRVRL SWGRSAKQTA LLQQAMLSNS LQVQQQQPGL QQPNYGYIPS 

       430        440        450        460        470        480 
STCEAPVLPD NNVSSTMLPG CQILNYSNPY ANANGLGSNN FSFYSNNNAT NTQATSLLAD 

       490        500        510        520 
TSSMDLSGTG GQQVIMQGSE AVVNSTNAML NRLEQGSNGF MFA 

« Hide

References

« Hide 'large scale' references
[1]"The NAM8 gene in Saccharomyces cerevisiae encodes a protein with putative RNA binding motifs and acts as a suppressor of mitochondrial splicing deficiencies when overexpressed."
Ekwall K., Kermorgant M., Dujardin G., Groudinsky O., Slonimski P.P.
Mol. Gen. Genet. 233:136-144(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: R23/50.
[2]"A yeast MRE2 gene that is essential for meiotic recombination encodes a novel ribonucleoprotein."
Leem S.-H., Hayashi A., Ajimura M., Ogawa H.
Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X64763 Genomic DNA. Translation: CAA46011.1.
D11461 Genomic DNA. Translation: BAA02016.1.
U00060 Genomic DNA. Translation: AAB68928.1.
BK006934 Genomic DNA. Translation: DAA06781.1.
PIRS46720.
RefSeqNP_011954.1. NM_001179216.1.

3D structure databases

ProteinModelPortalQ00539.
SMRQ00539. Positions 56-269, 309-384.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36521. 97 interactions.
DIPDIP-2749N.
IntActQ00539. 34 interactions.
MINTMINT-612683.
STRING4932.YHR086W.

Proteomic databases

MaxQBQ00539.
PaxDbQ00539.
PeptideAtlasQ00539.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR086W; YHR086W; YHR086W.
GeneID856486.
KEGGsce:YHR086W.

Organism-specific databases

CYGDYHR086w.
SGDS000001128. NAM8.

Phylogenomic databases

eggNOGCOG0724.
GeneTreeENSGT00510000047100.
HOGENOMHOG000066109.
OMAFPIANSR.
OrthoDBEOG7PZS6P.

Enzyme and pathway databases

BioCycYEAST:G3O-31133-MONOMER.

Gene expression databases

GenevestigatorQ00539.

Family and domain databases

Gene3D3.30.70.330. 3 hits.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTSM00360. RRM. 3 hits.
[Graphical view]
PROSITEPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio982182.

Entry information

Entry nameNAM8_YEAST
AccessionPrimary (citable) accession number: Q00539
Secondary accession number(s): D3DL37
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families