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Q00539

- NAM8_YEAST

UniProt

Q00539 - NAM8_YEAST

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Protein

Protein NAM8

Gene

NAM8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a suppressor of mitochondrial splicing deficiencies when overexpressed. Could be a non-essential component of the mitochondrial splicing machinery.

GO - Molecular functioni

  1. mRNA binding Source: SGD
  2. nucleotide binding Source: InterPro
  3. RNA binding Source: SGD

GO - Biological processi

  1. mRNA splice site selection Source: SGD
  2. mRNA splicing, via spliceosome Source: SGD
  3. positive regulation of mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31133-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NAM8
Gene namesi
Name:NAM8
Synonyms:MRE2
Ordered Locus Names:YHR086W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR086w.
SGDiS000001128. NAM8.

Subcellular locationi

GO - Cellular componenti

  1. commitment complex Source: SGD
  2. cytoplasm Source: SGD
  3. mitochondrion Source: UniProtKB-KW
  4. nucleus Source: SGD
  5. U1 snRNP Source: SGD
  6. U2-type prespliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 523523Protein NAM8PRO_0000081659Add
BLAST

Proteomic databases

MaxQBiQ00539.
PaxDbiQ00539.
PeptideAtlasiQ00539.

Expressioni

Gene expression databases

GenevestigatoriQ00539.

Interactioni

Protein-protein interaction databases

BioGridi36521. 98 interactions.
DIPiDIP-2749N.
IntActiQ00539. 34 interactions.
MINTiMINT-612683.
STRINGi4932.YHR086W.

Structurei

3D structure databases

ProteinModelPortaliQ00539.
SMRiQ00539. Positions 56-384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 14592RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini163 – 24280RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini313 – 38573RRM 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00510000047100.
HOGENOMiHOG000066109.
InParanoidiQ00539.
OMAiFPIANSR.
OrthoDBiEOG7PZS6P.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00539-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSYKQTTYYP SRGNLVRNDS SPYTNTISSE TNNSSTSVLS LQGASNVSLG
60 70 80 90 100
TTGNQLYMGD LDPTWDKNTV RQIWASLGEA NINVRMMWNN TLNNGSRSSM
110 120 130 140 150
GPKNNQGYCF VDFPSSTHAA NALLKNGMLI PNFPNKKLKL NWATSSYSNS
160 170 180 190 200
NNSLNNVKSG NNCSIFVGDL APNVTESQLF ELFINRYAST SHAKIVHDQV
210 220 230 240 250
TGMSKGYGFV KFTNSDEQQL ALSEMQGVFL NGRAIKVGPT SGQQQHVSGN
260 270 280 290 300
NDYNRSSSSL NNENVDSRFL SKGQSFLSNG NNNMGFKRNH MSQFIYPVQQ
310 320 330 340 350
QPSLNHFTDP NNTTVFIGGL SSLVTEDELR AYFQPFGTIV YVKIPVGKCC
360 370 380 390 400
GFVQYVDRLS AEAAIAGMQG FPIANSRVRL SWGRSAKQTA LLQQAMLSNS
410 420 430 440 450
LQVQQQQPGL QQPNYGYIPS STCEAPVLPD NNVSSTMLPG CQILNYSNPY
460 470 480 490 500
ANANGLGSNN FSFYSNNNAT NTQATSLLAD TSSMDLSGTG GQQVIMQGSE
510 520
AVVNSTNAML NRLEQGSNGF MFA
Length:523
Mass (Da):56,972
Last modified:February 1, 1995 - v2
Checksum:i64F198EEFB32A909
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti180 – 1801F → L in CAA46011. (PubMed:1603056)Curated
Sequence conflicti208 – 2092GF → VL in CAA46011. (PubMed:1603056)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64763 Genomic DNA. Translation: CAA46011.1.
D11461 Genomic DNA. Translation: BAA02016.1.
U00060 Genomic DNA. Translation: AAB68928.1.
BK006934 Genomic DNA. Translation: DAA06781.1.
PIRiS46720.
RefSeqiNP_011954.1. NM_001179216.1.

Genome annotation databases

EnsemblFungiiYHR086W; YHR086W; YHR086W.
GeneIDi856486.
KEGGisce:YHR086W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64763 Genomic DNA. Translation: CAA46011.1 .
D11461 Genomic DNA. Translation: BAA02016.1 .
U00060 Genomic DNA. Translation: AAB68928.1 .
BK006934 Genomic DNA. Translation: DAA06781.1 .
PIRi S46720.
RefSeqi NP_011954.1. NM_001179216.1.

3D structure databases

ProteinModelPortali Q00539.
SMRi Q00539. Positions 56-384.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36521. 98 interactions.
DIPi DIP-2749N.
IntActi Q00539. 34 interactions.
MINTi MINT-612683.
STRINGi 4932.YHR086W.

Proteomic databases

MaxQBi Q00539.
PaxDbi Q00539.
PeptideAtlasi Q00539.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR086W ; YHR086W ; YHR086W .
GeneIDi 856486.
KEGGi sce:YHR086W.

Organism-specific databases

CYGDi YHR086w.
SGDi S000001128. NAM8.

Phylogenomic databases

eggNOGi COG0724.
GeneTreei ENSGT00510000047100.
HOGENOMi HOG000066109.
InParanoidi Q00539.
OMAi FPIANSR.
OrthoDBi EOG7PZS6P.

Enzyme and pathway databases

BioCyci YEAST:G3O-31133-MONOMER.

Miscellaneous databases

NextBioi 982182.

Gene expression databases

Genevestigatori Q00539.

Family and domain databases

Gene3Di 3.30.70.330. 3 hits.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
Pfami PF00076. RRM_1. 2 hits.
[Graphical view ]
SMARTi SM00360. RRM. 3 hits.
[Graphical view ]
PROSITEi PS50102. RRM. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The NAM8 gene in Saccharomyces cerevisiae encodes a protein with putative RNA binding motifs and acts as a suppressor of mitochondrial splicing deficiencies when overexpressed."
    Ekwall K., Kermorgant M., Dujardin G., Groudinsky O., Slonimski P.P.
    Mol. Gen. Genet. 233:136-144(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: R23/50.
  2. "A yeast MRE2 gene that is essential for meiotic recombination encodes a novel ribonucleoprotein."
    Leem S.-H., Hayashi A., Ajimura M., Ogawa H.
    Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNAM8_YEAST
AccessioniPrimary (citable) accession number: Q00539
Secondary accession number(s): D3DL37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3