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Protein

Protein NAM8

Gene

NAM8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a suppressor of mitochondrial splicing deficiencies when overexpressed. Could be a non-essential component of the mitochondrial splicing machinery.

GO - Molecular functioni

  • mRNA binding Source: SGD
  • nucleotide binding Source: InterPro
  • RNA binding Source: SGD

GO - Biological processi

  • mRNA splice site selection Source: SGD
  • mRNA splicing, via spliceosome Source: SGD
  • positive regulation of mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31133-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NAM8
Gene namesi
Name:NAM8
Synonyms:MRE2
Ordered Locus Names:YHR086W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR086W.
SGDiS000001128. NAM8.

Subcellular locationi

GO - Cellular componenti

  • commitment complex Source: SGD
  • cytoplasm Source: SGD
  • mitochondrion Source: UniProtKB-SubCell
  • nucleus Source: SGD
  • U1 snRNP Source: SGD
  • U2-type prespliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816591 – 523Protein NAM8Add BLAST523

Proteomic databases

MaxQBiQ00539.
PRIDEiQ00539.

PTM databases

iPTMnetiQ00539.

Interactioni

Protein-protein interaction databases

BioGridi36521. 101 interactors.
DIPiDIP-2749N.
IntActiQ00539. 35 interactors.
MINTiMINT-612683.

Structurei

3D structure databases

ProteinModelPortaliQ00539.
SMRiQ00539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 145RRM 1PROSITE-ProRule annotationAdd BLAST92
Domaini163 – 242RRM 2PROSITE-ProRule annotationAdd BLAST80
Domaini313 – 385RRM 3PROSITE-ProRule annotationAdd BLAST73

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00510000047100.
HOGENOMiHOG000066109.
InParanoidiQ00539.
OrthoDBiEOG092C3SCB.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00539-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYKQTTYYP SRGNLVRNDS SPYTNTISSE TNNSSTSVLS LQGASNVSLG
60 70 80 90 100
TTGNQLYMGD LDPTWDKNTV RQIWASLGEA NINVRMMWNN TLNNGSRSSM
110 120 130 140 150
GPKNNQGYCF VDFPSSTHAA NALLKNGMLI PNFPNKKLKL NWATSSYSNS
160 170 180 190 200
NNSLNNVKSG NNCSIFVGDL APNVTESQLF ELFINRYAST SHAKIVHDQV
210 220 230 240 250
TGMSKGYGFV KFTNSDEQQL ALSEMQGVFL NGRAIKVGPT SGQQQHVSGN
260 270 280 290 300
NDYNRSSSSL NNENVDSRFL SKGQSFLSNG NNNMGFKRNH MSQFIYPVQQ
310 320 330 340 350
QPSLNHFTDP NNTTVFIGGL SSLVTEDELR AYFQPFGTIV YVKIPVGKCC
360 370 380 390 400
GFVQYVDRLS AEAAIAGMQG FPIANSRVRL SWGRSAKQTA LLQQAMLSNS
410 420 430 440 450
LQVQQQQPGL QQPNYGYIPS STCEAPVLPD NNVSSTMLPG CQILNYSNPY
460 470 480 490 500
ANANGLGSNN FSFYSNNNAT NTQATSLLAD TSSMDLSGTG GQQVIMQGSE
510 520
AVVNSTNAML NRLEQGSNGF MFA
Length:523
Mass (Da):56,972
Last modified:February 1, 1995 - v2
Checksum:i64F198EEFB32A909
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180F → L in CAA46011 (PubMed:1603056).Curated1
Sequence conflicti208 – 209GF → VL in CAA46011 (PubMed:1603056).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64763 Genomic DNA. Translation: CAA46011.1.
D11461 Genomic DNA. Translation: BAA02016.1.
U00060 Genomic DNA. Translation: AAB68928.1.
BK006934 Genomic DNA. Translation: DAA06781.1.
PIRiS46720.
RefSeqiNP_011954.1. NM_001179216.1.

Genome annotation databases

EnsemblFungiiYHR086W; YHR086W; YHR086W.
GeneIDi856486.
KEGGisce:YHR086W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64763 Genomic DNA. Translation: CAA46011.1.
D11461 Genomic DNA. Translation: BAA02016.1.
U00060 Genomic DNA. Translation: AAB68928.1.
BK006934 Genomic DNA. Translation: DAA06781.1.
PIRiS46720.
RefSeqiNP_011954.1. NM_001179216.1.

3D structure databases

ProteinModelPortaliQ00539.
SMRiQ00539.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36521. 101 interactors.
DIPiDIP-2749N.
IntActiQ00539. 35 interactors.
MINTiMINT-612683.

PTM databases

iPTMnetiQ00539.

Proteomic databases

MaxQBiQ00539.
PRIDEiQ00539.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR086W; YHR086W; YHR086W.
GeneIDi856486.
KEGGisce:YHR086W.

Organism-specific databases

EuPathDBiFungiDB:YHR086W.
SGDiS000001128. NAM8.

Phylogenomic databases

GeneTreeiENSGT00510000047100.
HOGENOMiHOG000066109.
InParanoidiQ00539.
OrthoDBiEOG092C3SCB.

Enzyme and pathway databases

BioCyciYEAST:G3O-31133-MONOMER.

Miscellaneous databases

PROiQ00539.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAM8_YEAST
AccessioniPrimary (citable) accession number: Q00539
Secondary accession number(s): D3DL37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.