Q00539 (NAM8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein NAM8

Gene names

Name:	NAM8
Synonyms:	MRE2
Ordered Locus Names:	YHR086W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a suppressor of mitochondrial splicing deficiencies when overexpressed. Could be a non-essential component of the mitochondrial splicing machinery.
Subcellular location	Mitochondrion.
Miscellaneous	Present with 1480 molecules/cell in log phase SD medium.
Sequence similarities	Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Mitochondrion
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splice site selection Inferred from mutant phenotype PubMed 10072385. Source: SGD positive regulation of mRNA splicing, via spliceosome Inferred from mutant phenotype PubMed 10983980. Source: SGD
Cellular_component	U1 snRNP Inferred from direct assay PubMed 9630245. Source: SGD U2-type prespliceosome Inferred from direct assay PubMed 16618970. Source: SGD commitment complex Inferred from physical interaction PubMed 10072386. Source: SGD mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mRNA binding Inferred from physical interaction PubMed 10072386. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 523	523	Protein NAM8		PRO_0000081659
Regions
	Domain	54 – 145	92	RRM 1
	Domain	163 – 242	80	RRM 2
	Domain	313 – 385	73	RRM 3
Amino acid modifications
	Modified residue	259	1	Phosphoserine Ref.6
	Modified residue	275	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	180	1	F → L in CAA46011. Ref.1
	Sequence conflict	208 – 209	2	GF → VL in CAA46011. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q00539 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 64F198EEFB32A909	FASTA	523	56,972
	10 20 30 40 50 60 MSYKQTTYYP SRGNLVRNDS SPYTNTISSE TNNSSTSVLS LQGASNVSLG TTGNQLYMGD 70 80 90 100 110 120 LDPTWDKNTV RQIWASLGEA NINVRMMWNN TLNNGSRSSM GPKNNQGYCF VDFPSSTHAA 130 140 150 160 170 180 NALLKNGMLI PNFPNKKLKL NWATSSYSNS NNSLNNVKSG NNCSIFVGDL APNVTESQLF 190 200 210 220 230 240 ELFINRYAST SHAKIVHDQV TGMSKGYGFV KFTNSDEQQL ALSEMQGVFL NGRAIKVGPT 250 260 270 280 290 300 SGQQQHVSGN NDYNRSSSSL NNENVDSRFL SKGQSFLSNG NNNMGFKRNH MSQFIYPVQQ 310 320 330 340 350 360 QPSLNHFTDP NNTTVFIGGL SSLVTEDELR AYFQPFGTIV YVKIPVGKCC GFVQYVDRLS 370 380 390 400 410 420 AEAAIAGMQG FPIANSRVRL SWGRSAKQTA LLQQAMLSNS LQVQQQQPGL QQPNYGYIPS 430 440 450 460 470 480 STCEAPVLPD NNVSSTMLPG CQILNYSNPY ANANGLGSNN FSFYSNNNAT NTQATSLLAD 490 500 510 520 TSSMDLSGTG GQQVIMQGSE AVVNSTNAML NRLEQGSNGF MFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The NAM8 gene in Saccharomyces cerevisiae encodes a protein with putative RNA binding motifs and acts as a suppressor of mitochondrial splicing deficiencies when overexpressed." Ekwall K., Kermorgant M., Dujardin G., Groudinsky O., Slonimski P.P. Mol. Gen. Genet. 233:136-144(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: R23/50.
[2]	"A yeast MRE2 gene that is essential for meiotic recombination encodes a novel ribonucleoprotein." Leem S.-H., Hayashi A., Ajimura M., Ogawa H. Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259 AND SER-275, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64763 Genomic DNA. Translation: CAA46011.1. D11461 Genomic DNA. Translation: BAA02016.1. U00060 Genomic DNA. Translation: AAB68928.1. BK006934 Genomic DNA. Translation: DAA06781.1.
PIR	S46720.
RefSeq	NP_011954.1. NM_001179216.1.
3D structure databases
ProteinModelPortal	Q00539.
SMR	Q00539. Positions 56-237, 309-384.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2749N.
IntAct	Q00539. 35 interactions.
MINT	MINT-612683.
STRING	4932.YHR086W.
Proteomic databases
PaxDb	Q00539.
PeptideAtlas	Q00539.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR086W; YHR086W; YHR086W.
GeneID	856486.
KEGG	sce:YHR086W.
Organism-specific databases
CYGD	YHR086w.
SGD	S000001128. NAM8.
Phylogenomic databases
eggNOG	COG0724.
GeneTree	ENSGT00510000047100.
HOGENOM	HOG000066109.
OMA	MNTGMNT.
OrthoDB	EOG44BFB8.
Gene expression databases
Genevestigator	Q00539.
GermOnline	YHR086W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.30.70.330. 3 hits.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 2 hits. [Graphical view]
SMART	SM00360. RRM. 3 hits. [Graphical view]
PROSITE	PS50102. RRM. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	982182.

Entry information

Entry name

NAM8_YEAST

Accession

Primary (citable) accession number: Q00539
Secondary accession number(s): D3DL37

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program