Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q00539

- NAM8_YEAST

UniProt

Q00539 - NAM8_YEAST

Protein

Protein NAM8

Gene

NAM8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Acts as a suppressor of mitochondrial splicing deficiencies when overexpressed. Could be a non-essential component of the mitochondrial splicing machinery.

    GO - Molecular functioni

    1. mRNA binding Source: SGD
    2. nucleotide binding Source: InterPro
    3. RNA binding Source: SGD

    GO - Biological processi

    1. mRNA splice site selection Source: SGD
    2. mRNA splicing, via spliceosome Source: SGD
    3. positive regulation of mRNA splicing, via spliceosome Source: SGD

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31133-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NAM8
    Gene namesi
    Name:NAM8
    Synonyms:MRE2
    Ordered Locus Names:YHR086W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR086w.
    SGDiS000001128. NAM8.

    Subcellular locationi

    GO - Cellular componenti

    1. commitment complex Source: SGD
    2. cytoplasm Source: SGD
    3. mitochondrion Source: UniProtKB-SubCell
    4. nucleus Source: SGD
    5. U1 snRNP Source: SGD
    6. U2-type prespliceosome Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 523523Protein NAM8PRO_0000081659Add
    BLAST

    Proteomic databases

    MaxQBiQ00539.
    PaxDbiQ00539.
    PeptideAtlasiQ00539.

    Expressioni

    Gene expression databases

    GenevestigatoriQ00539.

    Interactioni

    Protein-protein interaction databases

    BioGridi36521. 97 interactions.
    DIPiDIP-2749N.
    IntActiQ00539. 34 interactions.
    MINTiMINT-612683.
    STRINGi4932.YHR086W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00539.
    SMRiQ00539. Positions 56-269, 309-384.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 14592RRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini163 – 24280RRM 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini313 – 38573RRM 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0724.
    GeneTreeiENSGT00510000047100.
    HOGENOMiHOG000066109.
    OMAiFPIANSR.
    OrthoDBiEOG7PZS6P.

    Family and domain databases

    Gene3Di3.30.70.330. 3 hits.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF00076. RRM_1. 2 hits.
    [Graphical view]
    SMARTiSM00360. RRM. 3 hits.
    [Graphical view]
    PROSITEiPS50102. RRM. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00539-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYKQTTYYP SRGNLVRNDS SPYTNTISSE TNNSSTSVLS LQGASNVSLG    50
    TTGNQLYMGD LDPTWDKNTV RQIWASLGEA NINVRMMWNN TLNNGSRSSM 100
    GPKNNQGYCF VDFPSSTHAA NALLKNGMLI PNFPNKKLKL NWATSSYSNS 150
    NNSLNNVKSG NNCSIFVGDL APNVTESQLF ELFINRYAST SHAKIVHDQV 200
    TGMSKGYGFV KFTNSDEQQL ALSEMQGVFL NGRAIKVGPT SGQQQHVSGN 250
    NDYNRSSSSL NNENVDSRFL SKGQSFLSNG NNNMGFKRNH MSQFIYPVQQ 300
    QPSLNHFTDP NNTTVFIGGL SSLVTEDELR AYFQPFGTIV YVKIPVGKCC 350
    GFVQYVDRLS AEAAIAGMQG FPIANSRVRL SWGRSAKQTA LLQQAMLSNS 400
    LQVQQQQPGL QQPNYGYIPS STCEAPVLPD NNVSSTMLPG CQILNYSNPY 450
    ANANGLGSNN FSFYSNNNAT NTQATSLLAD TSSMDLSGTG GQQVIMQGSE 500
    AVVNSTNAML NRLEQGSNGF MFA 523
    Length:523
    Mass (Da):56,972
    Last modified:February 1, 1995 - v2
    Checksum:i64F198EEFB32A909
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti180 – 1801F → L in CAA46011. (PubMed:1603056)Curated
    Sequence conflicti208 – 2092GF → VL in CAA46011. (PubMed:1603056)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64763 Genomic DNA. Translation: CAA46011.1.
    D11461 Genomic DNA. Translation: BAA02016.1.
    U00060 Genomic DNA. Translation: AAB68928.1.
    BK006934 Genomic DNA. Translation: DAA06781.1.
    PIRiS46720.
    RefSeqiNP_011954.1. NM_001179216.1.

    Genome annotation databases

    EnsemblFungiiYHR086W; YHR086W; YHR086W.
    GeneIDi856486.
    KEGGisce:YHR086W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64763 Genomic DNA. Translation: CAA46011.1 .
    D11461 Genomic DNA. Translation: BAA02016.1 .
    U00060 Genomic DNA. Translation: AAB68928.1 .
    BK006934 Genomic DNA. Translation: DAA06781.1 .
    PIRi S46720.
    RefSeqi NP_011954.1. NM_001179216.1.

    3D structure databases

    ProteinModelPortali Q00539.
    SMRi Q00539. Positions 56-269, 309-384.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36521. 97 interactions.
    DIPi DIP-2749N.
    IntActi Q00539. 34 interactions.
    MINTi MINT-612683.
    STRINGi 4932.YHR086W.

    Proteomic databases

    MaxQBi Q00539.
    PaxDbi Q00539.
    PeptideAtlasi Q00539.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR086W ; YHR086W ; YHR086W .
    GeneIDi 856486.
    KEGGi sce:YHR086W.

    Organism-specific databases

    CYGDi YHR086w.
    SGDi S000001128. NAM8.

    Phylogenomic databases

    eggNOGi COG0724.
    GeneTreei ENSGT00510000047100.
    HOGENOMi HOG000066109.
    OMAi FPIANSR.
    OrthoDBi EOG7PZS6P.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31133-MONOMER.

    Miscellaneous databases

    NextBioi 982182.

    Gene expression databases

    Genevestigatori Q00539.

    Family and domain databases

    Gene3Di 3.30.70.330. 3 hits.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF00076. RRM_1. 2 hits.
    [Graphical view ]
    SMARTi SM00360. RRM. 3 hits.
    [Graphical view ]
    PROSITEi PS50102. RRM. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The NAM8 gene in Saccharomyces cerevisiae encodes a protein with putative RNA binding motifs and acts as a suppressor of mitochondrial splicing deficiencies when overexpressed."
      Ekwall K., Kermorgant M., Dujardin G., Groudinsky O., Slonimski P.P.
      Mol. Gen. Genet. 233:136-144(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: R23/50.
    2. "A yeast MRE2 gene that is essential for meiotic recombination encodes a novel ribonucleoprotein."
      Leem S.-H., Hayashi A., Ajimura M., Ogawa H.
      Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNAM8_YEAST
    AccessioniPrimary (citable) accession number: Q00539
    Secondary accession number(s): D3DL37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1480 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3