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Protein

Cyclin-dependent kinase 16

Gene

CDK16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).By similarity3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei194ATPCurated1
Active sitei286Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi171 – 179ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • exocytosis Source: UniProtKB
  • growth hormone secretion Source: UniProtKB
  • neuron projection development Source: UniProtKB
  • protein phosphorylation Source: ProtInc
  • regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  • spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS02369-MONOMER.
BRENDAi2.7.11.22. 2681.
SignaLinkiQ00536.
SIGNORiQ00536.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 16 (EC:2.7.11.22)
Alternative name(s):
Cell division protein kinase 16
PCTAIRE-motif protein kinase 1
Serine/threonine-protein kinase PCTAIRE-1
Gene namesi
Name:CDK16
Synonyms:PCTAIRE1, PCTK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:8749. CDK16.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  • microtubule cytoskeleton Source: HPA
  • neuron projection Source: UniProtKB-SubCell
  • plasma membrane Source: HPA
  • synaptic vesicle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12S → A: Abolishes phosphorylation by BRSK2. Abolishes effect on insulin secretion. 1 Publication1
Mutagenesisi119S → A: Strongly reduces interaction with CCNY. 1 Publication1
Mutagenesisi153S → A: Constitutively activated, due to loss of an inhibitory phosphorylation site. Increases interaction with CCNY. 2 Publications1
Mutagenesisi153S → D: Abolishes interaction with CCNY. 2 Publications1
Mutagenesisi194K → A: Loss of kinase activity. Abolishes effect on insulin secretion. 2 Publications1
Mutagenesisi194K → R: Loss of kinase activity. 2 Publications1

Organism-specific databases

DisGeNETi5127.
OpenTargetsiENSG00000102225.
PharmGKBiPA33095.

Chemistry databases

ChEMBLiCHEMBL4597.
GuidetoPHARMACOLOGYi1969.

Polymorphism and mutation databases

BioMutaiCDK16.
DMDMi266425.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000864841 – 496Cyclin-dependent kinase 16Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphoserine; by BRSK2Combined sources1 Publication1
Modified residuei42PhosphoserineCombined sources1
Modified residuei65PhosphoserineCombined sources1
Modified residuei78PhosphoserineCombined sources1
Modified residuei82PhosphoserineCombined sources1
Modified residuei95Phosphoserine; by CDK5Combined sources1
Modified residuei110PhosphoserineBy similarity1
Modified residuei119PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei153PhosphoserineCombined sources1
Modified residuei155PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-153 inhibits kinase activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ00536.
MaxQBiQ00536.
PaxDbiQ00536.
PeptideAtlasiQ00536.
PRIDEiQ00536.

PTM databases

iPTMnetiQ00536.
PhosphoSitePlusiQ00536.

Expressioni

Tissue specificityi

Detected in pancreas islets (at protein level). Detected in brain and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000102225.
CleanExiHS_PCTK1.
ExpressionAtlasiQ00536. baseline and differential.
GenevisibleiQ00536. HS.

Organism-specific databases

HPAiCAB016535.
HPA000974.
HPA001366.

Interactioni

Subunit structurei

Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with YWHAH, YWHAQ and YWHAZ. Interacts with NSF. Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1 (By similarity). Interacts with BRSK2. Interacts with CCNY; this increases the CDK16 kinase activity.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APPBP2Q926243EBI-726261,EBI-743771
CCNYQ8ND76-17EBI-726261,EBI-11615526
CCNYL1Q8N7R73EBI-726261,EBI-10103094
SEC23AQ154363EBI-726261,EBI-81088
ZBTB14O438293EBI-726261,EBI-10176632

Protein-protein interaction databases

BioGridi111154. 40 interactors.
IntActiQ00536. 29 interactors.
STRINGi9606.ENSP00000276052.

Chemistry databases

BindingDBiQ00536.

Structurei

Secondary structure

1496
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi164 – 173Combined sources10
Beta strandi175 – 184Combined sources10
Turni185 – 187Combined sources3
Beta strandi190 – 196Combined sources7
Helixi212 – 215Combined sources4
Beta strandi226 – 231Combined sources6
Beta strandi236 – 241Combined sources6
Beta strandi244 – 246Combined sources3
Helixi247 – 253Combined sources7
Turni254 – 256Combined sources3
Helixi260 – 279Combined sources20
Beta strandi282 – 286Combined sources5
Helixi289 – 291Combined sources3
Beta strandi292 – 294Combined sources3
Beta strandi300 – 302Combined sources3
Beta strandi304 – 309Combined sources6
Helixi325 – 327Combined sources3
Helixi330 – 333Combined sources4
Helixi342 – 357Combined sources16
Helixi367 – 378Combined sources12
Turni383 – 385Combined sources3
Helixi389 – 391Combined sources3
Helixi393 – 397Combined sources5
Helixi408 – 411Combined sources4
Helixi417 – 426Combined sources10
Helixi431 – 433Combined sources3
Helixi437 – 440Combined sources4
Helixi444 – 449Combined sources6
Beta strandi454 – 456Combined sources3
Helixi462 – 464Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MTLX-ray2.40A163-478[»]
ProteinModelPortaliQ00536.
SMRiQ00536.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini165 – 446Protein kinasePROSITE-ProRule annotationAdd BLAST282

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
GeneTreeiENSGT00790000122986.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ00536.
KOiK08820.
OMAiPMAGPIT.
OrthoDBiEOG091G0CH0.
PhylomeDBiQ00536.
TreeFamiTF106508.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q00536-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDRMKKIKRQ LSMTLRGGRG IDKTNGAPEQ IGLDESGGGG GSDPGEAPTR
60 70 80 90 100
AAPGELRSAR GPLSSAPEIV HEDLKMGSDG ESDQASATSS DEVQSPVRVR
110 120 130 140 150
MRNHPPRKIS TEDINKRLSL PADIRLPEGY LEKLTLNSPI FDKPLSRRLR
160 170 180 190 200
RVSLSEIGFG KLETYIKLDK LGEGTYATVY KGKSKLTDNL VALKEIRLEH
210 220 230 240 250
EEGAPCTAIR EVSLLKDLKH ANIVTLHDII HTEKSLTLVF EYLDKDLKQY
260 270 280 290 300
LDDCGNIINM HNVKLFLFQL LRGLAYCHRQ KVLHRDLKPQ NLLINERGEL
310 320 330 340 350
KLADFGLARA KSIPTKTYSN EVVTLWYRPP DILLGSTDYS TQIDMWGVGC
360 370 380 390 400
IFYEMATGRP LFPGSTVEEQ LHFIFRILGT PTEETWPGIL SNEEFKTYNY
410 420 430 440 450
PKYRAEALLS HAPRLDSDGA DLLTKLLQFE GRNRISAEDA MKHPFFLSLG
460 470 480 490
ERIHKLPDTT SIFALKEIQL QKEASLRSSS MPDSGRPAFR VVDTEF
Length:496
Mass (Da):55,716
Last modified:April 1, 1993 - v1
Checksum:i9A5DDD34A5E5CBBB
GO
Isoform 2 (identifier: Q00536-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MPLYGRARDH...HLNLRTQIAM

Note: No experimental confirmation available.
Show »
Length:570
Mass (Da):63,458
Checksum:i9D3020F5CADD1899
GO
Isoform 3 (identifier: Q00536-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MQSEIAM

Note: No experimental confirmation available.
Show »
Length:502
Mass (Da):56,375
Checksum:iAC4B7353DFF4D005
GO

Sequence cautioni

The sequence AAH01048 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH15607 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25N → D in BAH13564 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0461341M → MPLYGRARDHVTHPSILGTR PGRPMAGPITAAVPEKICNG AFCSCSGAFPLDPNNPSLGP LPSISHLNLRTQIAM in isoform 2. 1 Publication1
Alternative sequenceiVSP_0463421M → MQSEIAM in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66363 mRNA. Translation: CAA47006.1.
BT006827 mRNA. Translation: AAP35473.1.
AK290145 mRNA. Translation: BAF82834.1.
AK301832 mRNA. Translation: BAH13564.1.
AL096791 Genomic DNA. Translation: CAD20055.1.
AL513366 Genomic DNA. No translation available.
CH471164 Genomic DNA. Translation: EAW59295.1.
CH471164 Genomic DNA. Translation: EAW59297.1.
BC001048 mRNA. Translation: AAH01048.1. Different initiation.
BC015607 mRNA. Translation: AAH15607.1. Different initiation.
CCDSiCCDS14276.1. [Q00536-1]
CCDS48101.1. [Q00536-3]
CCDS55408.1. [Q00536-2]
PIRiS23385.
RefSeqiNP_001163931.1. NM_001170460.1. [Q00536-2]
NP_006192.1. NM_006201.4. [Q00536-1]
NP_148978.2. NM_033018.3. [Q00536-3]
XP_016885059.1. XM_017029570.1. [Q00536-1]
XP_016885060.1. XM_017029571.1. [Q00536-1]
XP_016885061.1. XM_017029572.1. [Q00536-1]
XP_016885062.1. XM_017029573.1. [Q00536-1]
UniGeneiHs.496068.

Genome annotation databases

EnsembliENST00000276052; ENSP00000276052; ENSG00000102225. [Q00536-2]
ENST00000357227; ENSP00000349762; ENSG00000102225. [Q00536-1]
ENST00000457458; ENSP00000405798; ENSG00000102225. [Q00536-3]
ENST00000518022; ENSP00000429751; ENSG00000102225. [Q00536-1]
GeneIDi5127.
KEGGihsa:5127.
UCSCiuc004dho.4. human. [Q00536-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66363 mRNA. Translation: CAA47006.1.
BT006827 mRNA. Translation: AAP35473.1.
AK290145 mRNA. Translation: BAF82834.1.
AK301832 mRNA. Translation: BAH13564.1.
AL096791 Genomic DNA. Translation: CAD20055.1.
AL513366 Genomic DNA. No translation available.
CH471164 Genomic DNA. Translation: EAW59295.1.
CH471164 Genomic DNA. Translation: EAW59297.1.
BC001048 mRNA. Translation: AAH01048.1. Different initiation.
BC015607 mRNA. Translation: AAH15607.1. Different initiation.
CCDSiCCDS14276.1. [Q00536-1]
CCDS48101.1. [Q00536-3]
CCDS55408.1. [Q00536-2]
PIRiS23385.
RefSeqiNP_001163931.1. NM_001170460.1. [Q00536-2]
NP_006192.1. NM_006201.4. [Q00536-1]
NP_148978.2. NM_033018.3. [Q00536-3]
XP_016885059.1. XM_017029570.1. [Q00536-1]
XP_016885060.1. XM_017029571.1. [Q00536-1]
XP_016885061.1. XM_017029572.1. [Q00536-1]
XP_016885062.1. XM_017029573.1. [Q00536-1]
UniGeneiHs.496068.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MTLX-ray2.40A163-478[»]
ProteinModelPortaliQ00536.
SMRiQ00536.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111154. 40 interactors.
IntActiQ00536. 29 interactors.
STRINGi9606.ENSP00000276052.

Chemistry databases

BindingDBiQ00536.
ChEMBLiCHEMBL4597.
GuidetoPHARMACOLOGYi1969.

PTM databases

iPTMnetiQ00536.
PhosphoSitePlusiQ00536.

Polymorphism and mutation databases

BioMutaiCDK16.
DMDMi266425.

Proteomic databases

EPDiQ00536.
MaxQBiQ00536.
PaxDbiQ00536.
PeptideAtlasiQ00536.
PRIDEiQ00536.

Protocols and materials databases

DNASUi5127.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000276052; ENSP00000276052; ENSG00000102225. [Q00536-2]
ENST00000357227; ENSP00000349762; ENSG00000102225. [Q00536-1]
ENST00000457458; ENSP00000405798; ENSG00000102225. [Q00536-3]
ENST00000518022; ENSP00000429751; ENSG00000102225. [Q00536-1]
GeneIDi5127.
KEGGihsa:5127.
UCSCiuc004dho.4. human. [Q00536-1]

Organism-specific databases

CTDi5127.
DisGeNETi5127.
GeneCardsiCDK16.
HGNCiHGNC:8749. CDK16.
HPAiCAB016535.
HPA000974.
HPA001366.
MIMi311550. gene.
neXtProtiNX_Q00536.
OpenTargetsiENSG00000102225.
PharmGKBiPA33095.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
GeneTreeiENSGT00790000122986.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ00536.
KOiK08820.
OMAiPMAGPIT.
OrthoDBiEOG091G0CH0.
PhylomeDBiQ00536.
TreeFamiTF106508.

Enzyme and pathway databases

BioCyciZFISH:HS02369-MONOMER.
BRENDAi2.7.11.22. 2681.
SignaLinkiQ00536.
SIGNORiQ00536.

Miscellaneous databases

ChiTaRSiCDK16. human.
GeneWikiiPCTK1.
GenomeRNAii5127.
PROiQ00536.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102225.
CleanExiHS_PCTK1.
ExpressionAtlasiQ00536. baseline and differential.
GenevisibleiQ00536. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDK16_HUMAN
AccessioniPrimary (citable) accession number: Q00536
Secondary accession number(s): A8K280
, B7Z7C8, J3KN74, J3KQP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 173 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.