Reviewed, UniProtKB/Swiss-Prot Q00531 (JI60_HORVU)

Names and origin

Protein names	Recommended name:     60 kDa jasmonate-induced protein     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Induction	By jasmonate.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	60 kDa jasmonate-induced protein		PRO_0000221400
Sites
	Active site	201	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q00531-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 7FD9BEAA024D4813	FASTA	560	60,362
	10 20 30 40 50 60 MALDKVAPIV IVTPFNVMTD RYDEFIEKVR KALAGTAGAK VGPKPKSKVE SPVLDKGTFP 70 80 90 100 110 120 VEQPPRWIHV ELHGKTQGTT TPKPKVAIRS DDAYIMGFTN STGRWFQLSK TGTTYKLVDD 130 140 150 160 170 180 KAVMAGFDGN YNTLVGGVNN LPTLNLNKFS MAQAAAALWN KASTLSGGIG SDVVDDDDGD 190 200 210 220 230 240 MLRANDPVKQ AVATLAVAVC EAARFSPVSK VVNAGWIKDK VSVTPDEVNY IKEWGDLSTA 250 260 270 280 290 300 LLSWMDKQYK DDATIFKKFN GIGITNGEEA LAVVRLVKLV IRSNMAAAPT TDEQLLAYAQ 310 320 330 340 350 360 LPKHGRYMAE VFAVRIPATA GGDRPAAPSL CTAATAAATS FTARKKNTPR SKPAATARVT 370 380 390 400 410 420 WSSLGHRLAT SAYGPIVFNL DLHDGNCGQA DEEEDEKNTG RIVCDAIGGD FSNYNKAISE 430 440 450 460 470 480 TVLTRCGPAE VIYAVLSNGV QGRVDVKLAG LQSRDEVVLV GRIVARSKLF DFGCVLFYNE 490 500 510 520 530 540 AAGVRVRPGE LVPLARHALA VPLHMPLTIE LDIRHGGSGD EIVRGELEFK TAIDGLHTGR 550 560 LVGVNDAEFE VTILWSEYPW

References

[1]	"Isolation and characterization of a cDNA clone encoding a novel jasmonate-induced protein of barley (Hordeum vulgare L.)." Becker W., Apel K. Plant Mol. Biol. 19:1065-1067(1992) [PubMed: 1511131] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66376 mRNA. Translation: CAA47017.1.
PIR	S25092.
UniGene	Hv.24485
3D structure databases
SMR	Q00531. Positions 14-278.
ModBase	Search...
Organism-specific databases
Gramene	Q00531.
Enzyme and pathway databases
BRENDA	3.2.2.22. 283.
Gene expression databases
Genevestigator	Q00531.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JI60_HORVU

Accession

Primary (citable) accession number: Q00531

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)