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Protein

Cyclin-dependent kinase 3

Gene

CDK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: ProtInc

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • cell proliferation Source: UniProtKB
  • G0 to G1 transition Source: UniProtKB
  • G1/S transition of mitotic cell cycle Source: UniProtKB
  • mitotic nuclear division Source: UniProtKB-KW
  • regulation of cell cycle Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 2681.
SignaLinkiQ00526.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 3 (EC:2.7.11.22)
Alternative name(s):
Cell division protein kinase 3
Gene namesi
Name:CDK3
Synonyms:CDKN3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:1772. CDK3.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26309.

Polymorphism and mutation databases

BioMutaiCDK3.
DMDMi231726.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Cyclin-dependent kinase 3PRO_0000085776Add
BLAST

Proteomic databases

MaxQBiQ00526.
PaxDbiQ00526.
PRIDEiQ00526.

PTM databases

PhosphoSiteiQ00526.

Expressioni

Tissue specificityi

Expressed in cancer cell lines and glioblastoma tissue.1 Publication

Gene expression databases

BgeeiQ00526.
CleanExiHS_CDK3.
ExpressionAtlasiQ00526. baseline.
GenevisibleiQ00526. HS.

Organism-specific databases

HPAiHPA007420.

Interactioni

Subunit structurei

Interacts with CABLES1 and CABLES2 (By similarity). Interacts with ATF1. Binding to CCNC/cyclin-C promotes RB1 phosphorylation.By similarity2 Publications

Protein-protein interaction databases

BioGridi107453. 43 interactions.
DIPiDIP-686N.
IntActiQ00526. 23 interactions.
MINTiMINT-1509509.
STRINGi9606.ENSP00000400088.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LFNmodel-A1-305[»]
ProteinModelPortaliQ00526.
SMRiQ00526. Positions 1-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 286283Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00790000122973.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ00526.
KOiK02088.
OMAiRHTNETI.
OrthoDBiEOG7966H8.
PhylomeDBiQ00526.
TreeFamiTF101021.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDMFQKVEKI GEGTYGVVYK AKNRETGQLV ALKKIRLDLE MEGVPSTAIR
60 70 80 90 100
EISLLKELKH PNIVRLLDVV HNERKLYLVF EFLSQDLKKY MDSTPGSELP
110 120 130 140 150
LHLIKSYLFQ LLQGVSFCHS HRVIHRDLKP QNLLINELGA IKLADFGLAR
160 170 180 190 200
AFGVPLRTYT HEVVTLWYRA PEILLGSKFY TTAVDIWSIG CIFAEMVTRK
210 220 230 240 250
ALFPGDSEID QLFRIFRMLG TPSEDTWPGV TQLPDYKGSF PKWTRKGLEE
260 270 280 290 300
IVPNLEPEGR DLLMQLLQYD PSQRITAKTA LAHPYFSSPE PSPAARQYVL

QRFRH
Length:305
Mass (Da):35,046
Last modified:April 1, 1993 - v1
Checksum:i1128BE0096EA262A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti106 – 1061S → N in a glioblastoma multiforme sample; somatic mutation. 1 Publication
VAR_041973
Natural varianti124 – 1241I → T.1 Publication
Corresponds to variant rs34918446 [ dbSNP | Ensembl ].
VAR_041974
Natural varianti214 – 2141R → H.1 Publication
Corresponds to variant rs34670267 [ dbSNP | Ensembl ].
VAR_041975
Natural varianti226 – 2261T → I.1 Publication
Corresponds to variant rs2069532 [ dbSNP | Ensembl ].
VAR_021101
Natural varianti264 – 2641M → T.2 Publications
Corresponds to variant rs17884251 [ dbSNP | Ensembl ].
VAR_021102

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66357 mRNA. Translation: CAA47001.1.
AY789470 Genomic DNA. Translation: AAV40830.1.
CCDSiCCDS11736.1.
PIRiS23382.
RefSeqiNP_001249.1. NM_001258.2.
UniGeneiHs.706766.

Genome annotation databases

EnsembliENST00000425876; ENSP00000410561; ENSG00000250506.
GeneIDi1018.
KEGGihsa:1018.
UCSCiuc010dgt.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66357 mRNA. Translation: CAA47001.1.
AY789470 Genomic DNA. Translation: AAV40830.1.
CCDSiCCDS11736.1.
PIRiS23382.
RefSeqiNP_001249.1. NM_001258.2.
UniGeneiHs.706766.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LFNmodel-A1-305[»]
ProteinModelPortaliQ00526.
SMRiQ00526. Positions 1-286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107453. 43 interactions.
DIPiDIP-686N.
IntActiQ00526. 23 interactions.
MINTiMINT-1509509.
STRINGi9606.ENSP00000400088.

Chemistry

BindingDBiQ00526.
ChEMBLiCHEMBL2111444.
GuidetoPHARMACOLOGYi1975.

PTM databases

PhosphoSiteiQ00526.

Polymorphism and mutation databases

BioMutaiCDK3.
DMDMi231726.

Proteomic databases

MaxQBiQ00526.
PaxDbiQ00526.
PRIDEiQ00526.

Protocols and materials databases

DNASUi1018.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000425876; ENSP00000410561; ENSG00000250506.
GeneIDi1018.
KEGGihsa:1018.
UCSCiuc010dgt.3. human.

Organism-specific databases

CTDi1018.
GeneCardsiGC17P073996.
HGNCiHGNC:1772. CDK3.
HPAiHPA007420.
MIMi123828. gene.
neXtProtiNX_Q00526.
PharmGKBiPA26309.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00790000122973.
HOGENOMiHOG000233024.
HOVERGENiHBG014652.
InParanoidiQ00526.
KOiK02088.
OMAiRHTNETI.
OrthoDBiEOG7966H8.
PhylomeDBiQ00526.
TreeFamiTF101021.

Enzyme and pathway databases

BRENDAi2.7.11.22. 2681.
SignaLinkiQ00526.

Miscellaneous databases

GeneWikiiCyclin-dependent_kinase_3.
GenomeRNAii1018.
NextBioi4279.
PROiQ00526.
SOURCEiSearch...

Gene expression databases

BgeeiQ00526.
CleanExiHS_CDK3.
ExpressionAtlasiQ00526. baseline.
GenevisibleiQ00526. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A family of human cdc2-related protein kinases."
    Meyerson M., Enders G.H., Wu C.-L., Su L.-K., Gorka C., Nelson C., Harlow E., Tsai L.-H.
    EMBO J. 11:2909-2917(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fetal brain.
  2. NIEHS SNPs program
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ILE-226 AND THR-264.
  3. "Differential effects of cdk2 and cdk3 on the control of pRb and E2F function during G1 exit."
    Hofmann F., Livingston D.M.
    Genes Dev. 10:851-861(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN G1-S TRANSITION.
  4. "Cyclin C/cdk3 promotes Rb-dependent G0 exit."
    Ren S., Rollins B.J.
    Cell 117:239-251(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS RB1 KINASE, FUNCTION IN G0-G1 TRANSITION, INTERACTION WITH CCNC.
  5. "Cyclin-dependent kinase 3-mediated activating transcription factor 1 phosphorylation enhances cell transformation."
    Zheng D., Cho Y.-Y., Lau A.T.Y., Zhang J., Ma W.-Y., Bode A.M., Dong Z.
    Cancer Res. 68:7650-7660(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS ATF1 KINASE, TISSUE SPECIFICITY, INTERACTION WITH ATF1.
  6. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASN-106; THR-124; HIS-214 AND THR-264.

Entry informationi

Entry nameiCDK3_HUMAN
AccessioniPrimary (citable) accession number: Q00526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 22, 2015
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.