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Q00526

- CDK3_HUMAN

UniProt

Q00526 - CDK3_HUMAN

Protein

Cyclin-dependent kinase 3

Gene

CDK3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 153 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.3 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: ProtInc

    GO - Biological processi

    1. cell proliferation Source: UniProtKB
    2. G0 to G1 transition Source: UniProtKB
    3. G1/S transition of mitotic cell cycle Source: UniProtKB
    4. mitotic nuclear division Source: UniProtKB-KW
    5. regulation of cell cycle Source: GOC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 2681.
    SignaLinkiQ00526.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 3 (EC:2.7.11.22)
    Alternative name(s):
    Cell division protein kinase 3
    Gene namesi
    Name:CDK3
    Synonyms:CDKN3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:1772. CDK3.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26309.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 305305Cyclin-dependent kinase 3PRO_0000085776Add
    BLAST

    Proteomic databases

    PaxDbiQ00526.
    PRIDEiQ00526.

    PTM databases

    PhosphoSiteiQ00526.

    Expressioni

    Tissue specificityi

    Expressed in cancer cell lines and glioblastoma tissue.1 Publication

    Gene expression databases

    BgeeiQ00526.
    CleanExiHS_CDK3.
    GenevestigatoriQ00526.

    Organism-specific databases

    HPAiHPA007420.

    Interactioni

    Subunit structurei

    Interacts with CABLES1 and CABLES2 By similarity. Interacts with ATF1. Binding to CCNC/cyclin-C promotes RB1 phosphorylation.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi107453. 32 interactions.
    DIPiDIP-686N.
    IntActiQ00526. 23 interactions.
    MINTiMINT-1509509.
    STRINGi9606.ENSP00000400088.

    Structurei

    Secondary structure

    1
    305
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 129
    Beta strandi14 – 2310
    Turni24 – 263
    Beta strandi29 – 368
    Beta strandi39 – 424
    Helixi46 – 5712
    Beta strandi66 – 716
    Beta strandi73 – 819
    Beta strandi84 – 863
    Helixi87 – 937
    Turni95 – 973
    Helixi101 – 12020
    Helixi130 – 1323
    Beta strandi133 – 1353
    Beta strandi141 – 1433
    Beta strandi154 – 1574
    Turni159 – 1613
    Helixi171 – 1755
    Helixi183 – 19816
    Helixi208 – 21912
    Turni224 – 2263
    Helixi230 – 2323
    Helixi248 – 2514
    Helixi257 – 26610
    Turni271 – 2733
    Helixi277 – 2826
    Helixi284 – 2863

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LFNmodel-A1-305[»]
    ProteinModelPortaliQ00526.
    SMRiQ00526. Positions 1-286.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 286283Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    HOVERGENiHBG014652.
    InParanoidiQ00526.
    KOiK02088.
    OMAiMVIRRAL.
    OrthoDBiEOG7966H8.
    PhylomeDBiQ00526.
    TreeFamiTF101021.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00526-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDMFQKVEKI GEGTYGVVYK AKNRETGQLV ALKKIRLDLE MEGVPSTAIR    50
    EISLLKELKH PNIVRLLDVV HNERKLYLVF EFLSQDLKKY MDSTPGSELP 100
    LHLIKSYLFQ LLQGVSFCHS HRVIHRDLKP QNLLINELGA IKLADFGLAR 150
    AFGVPLRTYT HEVVTLWYRA PEILLGSKFY TTAVDIWSIG CIFAEMVTRK 200
    ALFPGDSEID QLFRIFRMLG TPSEDTWPGV TQLPDYKGSF PKWTRKGLEE 250
    IVPNLEPEGR DLLMQLLQYD PSQRITAKTA LAHPYFSSPE PSPAARQYVL 300
    QRFRH 305
    Length:305
    Mass (Da):35,046
    Last modified:April 1, 1993 - v1
    Checksum:i1128BE0096EA262A
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti106 – 1061S → N in a glioblastoma multiforme sample; somatic mutation. 1 Publication
    VAR_041973
    Natural varianti124 – 1241I → T.1 Publication
    Corresponds to variant rs34918446 [ dbSNP | Ensembl ].
    VAR_041974
    Natural varianti214 – 2141R → H.1 Publication
    Corresponds to variant rs34670267 [ dbSNP | Ensembl ].
    VAR_041975
    Natural varianti226 – 2261T → I.1 Publication
    Corresponds to variant rs2069532 [ dbSNP | Ensembl ].
    VAR_021101
    Natural varianti264 – 2641M → T.2 Publications
    Corresponds to variant rs17884251 [ dbSNP | Ensembl ].
    VAR_021102

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66357 mRNA. Translation: CAA47001.1.
    AY789470 Genomic DNA. Translation: AAV40830.1.
    CCDSiCCDS11736.1.
    PIRiS23382.
    RefSeqiNP_001249.1. NM_001258.2.
    UniGeneiHs.706766.

    Genome annotation databases

    EnsembliENST00000425876; ENSP00000410561; ENSG00000250506.
    GeneIDi1018.
    KEGGihsa:1018.
    UCSCiuc010dgt.3. human.

    Polymorphism databases

    DMDMi231726.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66357 mRNA. Translation: CAA47001.1 .
    AY789470 Genomic DNA. Translation: AAV40830.1 .
    CCDSi CCDS11736.1.
    PIRi S23382.
    RefSeqi NP_001249.1. NM_001258.2.
    UniGenei Hs.706766.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LFN model - A 1-305 [» ]
    ProteinModelPortali Q00526.
    SMRi Q00526. Positions 1-286.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107453. 32 interactions.
    DIPi DIP-686N.
    IntActi Q00526. 23 interactions.
    MINTi MINT-1509509.
    STRINGi 9606.ENSP00000400088.

    Chemistry

    BindingDBi Q00526.
    ChEMBLi CHEMBL2111444.
    GuidetoPHARMACOLOGYi 1975.

    PTM databases

    PhosphoSitei Q00526.

    Polymorphism databases

    DMDMi 231726.

    Proteomic databases

    PaxDbi Q00526.
    PRIDEi Q00526.

    Protocols and materials databases

    DNASUi 1018.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000425876 ; ENSP00000410561 ; ENSG00000250506 .
    GeneIDi 1018.
    KEGGi hsa:1018.
    UCSCi uc010dgt.3. human.

    Organism-specific databases

    CTDi 1018.
    GeneCardsi GC17P073996.
    HGNCi HGNC:1772. CDK3.
    HPAi HPA007420.
    MIMi 123828. gene.
    neXtProti NX_Q00526.
    PharmGKBi PA26309.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    HOVERGENi HBG014652.
    InParanoidi Q00526.
    KOi K02088.
    OMAi MVIRRAL.
    OrthoDBi EOG7966H8.
    PhylomeDBi Q00526.
    TreeFami TF101021.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 2681.
    SignaLinki Q00526.

    Miscellaneous databases

    GeneWikii Cyclin-dependent_kinase_3.
    GenomeRNAii 1018.
    NextBioi 4279.
    PROi Q00526.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q00526.
    CleanExi HS_CDK3.
    Genevestigatori Q00526.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A family of human cdc2-related protein kinases."
      Meyerson M., Enders G.H., Wu C.-L., Su L.-K., Gorka C., Nelson C., Harlow E., Tsai L.-H.
      EMBO J. 11:2909-2917(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Fetal brain.
    2. NIEHS SNPs program
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ILE-226 AND THR-264.
    3. "Differential effects of cdk2 and cdk3 on the control of pRb and E2F function during G1 exit."
      Hofmann F., Livingston D.M.
      Genes Dev. 10:851-861(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN G1-S TRANSITION.
    4. "Cyclin C/cdk3 promotes Rb-dependent G0 exit."
      Ren S., Rollins B.J.
      Cell 117:239-251(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS RB1 KINASE, FUNCTION IN G0-G1 TRANSITION, INTERACTION WITH CCNC.
    5. "Cyclin-dependent kinase 3-mediated activating transcription factor 1 phosphorylation enhances cell transformation."
      Zheng D., Cho Y.-Y., Lau A.T.Y., Zhang J., Ma W.-Y., Bode A.M., Dong Z.
      Cancer Res. 68:7650-7660(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS ATF1 KINASE, TISSUE SPECIFICITY, INTERACTION WITH ATF1.
    6. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASN-106; THR-124; HIS-214 AND THR-264.

    Entry informationi

    Entry nameiCDK3_HUMAN
    AccessioniPrimary (citable) accession number: Q00526
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 153 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3