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Q00526 (CDK3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 151. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 3

EC=2.7.11.22
Alternative name(s):
Cell division protein kinase 3
Gene names
Name:CDK3
Synonyms:CDKN3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner. Ref.3 Ref.4 Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with CABLES1 and CABLES2 By similarity. Interacts with ATF1. Binding to CCNC/cyclin-C promotes RB1 phosphorylation. Ref.4 Ref.5

Tissue specificity

Expressed in cancer cell lines and glioblastoma tissue. Ref.5

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305Cyclin-dependent kinase 3
PRO_0000085776

Regions

Domain4 – 286283Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1271Proton acceptor By similarity
Binding site331ATP By similarity

Natural variations

Natural variant1061S → N in a glioblastoma multiforme sample; somatic mutation. Ref.6
VAR_041973
Natural variant1241I → T. Ref.6
Corresponds to variant rs34918446 [ dbSNP | Ensembl ].
VAR_041974
Natural variant2141R → H. Ref.6
Corresponds to variant rs34670267 [ dbSNP | Ensembl ].
VAR_041975
Natural variant2261T → I. Ref.2
Corresponds to variant rs2069532 [ dbSNP | Ensembl ].
VAR_021101
Natural variant2641M → T. Ref.2 Ref.6
Corresponds to variant rs17884251 [ dbSNP | Ensembl ].
VAR_021102

Secondary structure

.................................................... 305
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q00526 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 1128BE0096EA262A

FASTA30535,046
        10         20         30         40         50         60 
MDMFQKVEKI GEGTYGVVYK AKNRETGQLV ALKKIRLDLE MEGVPSTAIR EISLLKELKH 

        70         80         90        100        110        120 
PNIVRLLDVV HNERKLYLVF EFLSQDLKKY MDSTPGSELP LHLIKSYLFQ LLQGVSFCHS 

       130        140        150        160        170        180 
HRVIHRDLKP QNLLINELGA IKLADFGLAR AFGVPLRTYT HEVVTLWYRA PEILLGSKFY 

       190        200        210        220        230        240 
TTAVDIWSIG CIFAEMVTRK ALFPGDSEID QLFRIFRMLG TPSEDTWPGV TQLPDYKGSF 

       250        260        270        280        290        300 
PKWTRKGLEE IVPNLEPEGR DLLMQLLQYD PSQRITAKTA LAHPYFSSPE PSPAARQYVL 


QRFRH 

« Hide

References

« Hide 'large scale' references
[1]"A family of human cdc2-related protein kinases."
Meyerson M., Enders G.H., Wu C.-L., Su L.-K., Gorka C., Nelson C., Harlow E., Tsai L.-H.
EMBO J. 11:2909-2917(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fetal brain.
[2]NIEHS SNPs program
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ILE-226 AND THR-264.
[3]"Differential effects of cdk2 and cdk3 on the control of pRb and E2F function during G1 exit."
Hofmann F., Livingston D.M.
Genes Dev. 10:851-861(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN G1-S TRANSITION.
[4]"Cyclin C/cdk3 promotes Rb-dependent G0 exit."
Ren S., Rollins B.J.
Cell 117:239-251(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS RB1 KINASE, FUNCTION IN G0-G1 TRANSITION, INTERACTION WITH CCNC.
[5]"Cyclin-dependent kinase 3-mediated activating transcription factor 1 phosphorylation enhances cell transformation."
Zheng D., Cho Y.-Y., Lau A.T.Y., Zhang J., Ma W.-Y., Bode A.M., Dong Z.
Cancer Res. 68:7650-7660(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS ATF1 KINASE, TISSUE SPECIFICITY, INTERACTION WITH ATF1.
[6]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASN-106; THR-124; HIS-214 AND THR-264.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X66357 mRNA. Translation: CAA47001.1.
AY789470 Genomic DNA. Translation: AAV40830.1.
CCDSCCDS11736.1.
PIRS23382.
RefSeqNP_001249.1. NM_001258.2.
UniGeneHs.706766.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LFNmodel-A1-305[»]
ProteinModelPortalQ00526.
SMRQ00526. Positions 1-286.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107453. 32 interactions.
DIPDIP-686N.
IntActQ00526. 23 interactions.
MINTMINT-1509509.
STRING9606.ENSP00000400088.

Chemistry

BindingDBQ00526.
ChEMBLCHEMBL2111444.
GuidetoPHARMACOLOGY1975.

PTM databases

PhosphoSiteQ00526.

Polymorphism databases

DMDM231726.

Proteomic databases

PaxDbQ00526.
PRIDEQ00526.

Protocols and materials databases

DNASU1018.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000425876; ENSP00000410561; ENSG00000250506.
ENST00000448471; ENSP00000400088; ENSG00000250506.
GeneID1018.
KEGGhsa:1018.
UCSCuc010dgt.3. human.

Organism-specific databases

CTD1018.
GeneCardsGC17P073996.
HGNCHGNC:1772. CDK3.
HPAHPA007420.
MIM123828. gene.
neXtProtNX_Q00526.
PharmGKBPA26309.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
HOVERGENHBG014652.
InParanoidQ00526.
KOK02088.
OMAMVIRRAL.
OrthoDBEOG7966H8.
PhylomeDBQ00526.
TreeFamTF101021.

Enzyme and pathway databases

BRENDA2.7.11.22. 2681.
SignaLinkQ00526.

Gene expression databases

BgeeQ00526.
CleanExHS_CDK3.
GenevestigatorQ00526.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCyclin-dependent_kinase_3.
GenomeRNAi1018.
NextBio4279.
PROQ00526.
SOURCESearch...

Entry information

Entry nameCDK3_HUMAN
AccessionPrimary (citable) accession number: Q00526
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 9, 2014
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM