Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q00519

- XDH_MOUSE

UniProt

Q00519 - XDH_MOUSE

Protein

Xanthine dehydrogenase/oxidase

Gene

Xdh

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 5 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species By similarity.By similarity

    Catalytic activityi

    Xanthine + NAD+ + H2O = urate + NADH.
    Hypoxanthine + NAD+ + H2O = xanthine + NADH.
    Xanthine + H2O + O2 = urate + H2O2.

    Cofactori

    Binds 2 2Fe-2S clusters.By similarity
    FAD.By similarity
    Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

    Enzyme regulationi

    Can be converted from the dehydrogenase form (D) to the oxidase form (O) irreversibly by proteolysis or reversibly through the oxidation of sulfhydryl groups.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi46 – 461Iron-sulfur 1By similarity
    Metal bindingi51 – 511Iron-sulfur 1By similarity
    Metal bindingi54 – 541Iron-sulfur 1By similarity
    Metal bindingi76 – 761Iron-sulfur 1By similarity
    Metal bindingi115 – 1151Iron-sulfur 2By similarity
    Metal bindingi118 – 1181Iron-sulfur 2By similarity
    Metal bindingi150 – 1501Iron-sulfur 2By similarity
    Metal bindingi152 – 1521Iron-sulfur 2By similarity
    Binding sitei339 – 3391FADBy similarity
    Binding sitei362 – 3621FADBy similarity
    Binding sitei406 – 4061FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei424 – 4241FADBy similarity
    Metal bindingi770 – 7701MolybdenumBy similarity
    Metal bindingi801 – 8011Molybdenum; via carbonyl oxygenBy similarity
    Binding sitei805 – 8051SubstrateBy similarity
    Binding sitei883 – 8831SubstrateBy similarity
    Metal bindingi915 – 9151Molybdenum; via amide nitrogenBy similarity
    Binding sitei917 – 9171SubstrateBy similarity
    Binding sitei1013 – 10131Substrate; via amide nitrogenBy similarity
    Metal bindingi1082 – 10821Molybdenum; via amide nitrogenBy similarity
    Active sitei1264 – 12641Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi259 – 2668FADBy similarity
    Nucleotide bindingi349 – 3535FADBy similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB
    2. electron carrier activity Source: InterPro
    3. flavin adenine dinucleotide binding Source: UniProtKB
    4. iron ion binding Source: InterPro
    5. molybdenum ion binding Source: InterPro
    6. molybdopterin cofactor binding Source: UniProtKB
    7. oxidoreductase activity Source: MGI
    8. protein binding Source: MGI
    9. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
    10. xanthine dehydrogenase activity Source: UniProtKB
    11. xanthine oxidase activity Source: UniProtKB

    GO - Biological processi

    1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
    2. lactation Source: MGI
    3. negative regulation of endothelial cell differentiation Source: Ensembl
    4. negative regulation of endothelial cell proliferation Source: Ensembl
    5. negative regulation of gene expression Source: Ensembl
    6. negative regulation of protein kinase B signaling Source: Ensembl
    7. negative regulation of protein phosphorylation Source: Ensembl
    8. negative regulation of vascular endothelial growth factor signaling pathway Source: Ensembl
    9. negative regulation of vasculogenesis Source: Ensembl
    10. oxidation-reduction process Source: MGI
    11. positive regulation of p38MAPK cascade Source: Ensembl
    12. positive regulation of reactive oxygen species metabolic process Source: Ensembl
    13. regulation of epithelial cell differentiation Source: MGI
    14. xanthine catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14019.
    SABIO-RKQ00519.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xanthine dehydrogenase/oxidase
    Including the following 2 domains:
    Xanthine dehydrogenase (EC:1.17.1.4)
    Short name:
    XD
    Xanthine oxidase (EC:1.17.3.2)
    Short name:
    XO
    Alternative name(s):
    Xanthine oxidoreductase
    Short name:
    XOR
    Gene namesi
    Name:Xdh
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:98973. Xdh.

    Subcellular locationi

    Cytoplasm By similarity. Peroxisome By similarity. Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. peroxisome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Peroxisome, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 13351334Xanthine dehydrogenase/oxidasePRO_0000166085Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi538 ↔ 995In oxidase formBy similarity
    Glycosylationi1076 – 10761N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).By similarity
    Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ00519.
    PaxDbiQ00519.
    PRIDEiQ00519.

    PTM databases

    PhosphoSiteiQ00519.

    Expressioni

    Inductioni

    By interferon.

    Gene expression databases

    ArrayExpressiQ00519.
    BgeeiQ00519.
    CleanExiMM_XDH.
    GenevestigatoriQ00519.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with BTN1A1.1 Publication

    Protein-protein interaction databases

    IntActiQ00519. 2 interactions.
    MINTiMINT-1866314.
    STRINGi10090.ENSMUSP00000024866.

    Structurei

    3D structure databases

    ProteinModelPortaliQ00519.
    SMRiQ00519. Positions 7-166, 197-530, 574-1318.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 94882Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini231 – 416186FAD-binding PCMH-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the xanthine dehydrogenase family.Curated
    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG4630.
    GeneTreeiENSGT00390000003772.
    HOGENOMiHOG000191197.
    HOVERGENiHBG004182.
    InParanoidiQ00519.
    KOiK00106.
    OMAiCDETFFA.
    OrthoDBiEOG7QRQSZ.
    TreeFamiTF353036.

    Family and domain databases

    Gene3Di1.10.150.120. 1 hit.
    3.10.20.30. 1 hit.
    3.30.365.10. 6 hits.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    3.90.1170.50. 1 hit.
    InterProiIPR002888. 2Fe-2S-bd.
    IPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR000674. Ald_Oxase/Xan_DH_a/b.
    IPR016208. Ald_Oxase/xanthine_DH.
    IPR008274. AldOxase/xan_DH_Mopterin-bd.
    IPR012675. Beta-grasp_dom.
    IPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    IPR022407. OxRdtase_Mopterin_BS.
    IPR014309. Xanthine_DH_Mopterin-bd_su.
    IPR014307. Xanthine_DH_ssu.
    [Graphical view]
    PfamiPF01315. Ald_Xan_dh_C. 1 hit.
    PF02738. Ald_Xan_dh_C2. 1 hit.
    PF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    PF00111. Fer2. 1 hit.
    PF01799. Fer2_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
    SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
    SM01092. CO_deh_flav_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF47741. SSF47741. 1 hit.
    SSF54292. SSF54292. 1 hit.
    SSF54665. SSF54665. 1 hit.
    SSF55447. SSF55447. 1 hit.
    SSF56003. SSF56003. 1 hit.
    SSF56176. SSF56176. 1 hit.
    TIGRFAMsiTIGR02963. xanthine_xdhA. 1 hit.
    TIGR02965. xanthine_xdhB. 1 hit.
    PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS51387. FAD_PCMH. 1 hit.
    PS00559. MOLYBDOPTERIN_EUK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q00519-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTRTTVDELV FFVNGKKVVE KNADPETTLL VYLRRKLGLC GTKLGCGEGG     50
    CGACTVMISK YDRLQNKIVH FSVNACLTPI CSLHHVAVTT VEGIGNTKKL 100
    HPVQERIAKS HGSQCGFCTP GIVMSMYTLL RNKPEPTVEE IENAFQGNLC 150
    RCTGYRPILQ GFRTFAKDGG CCGGSGNNPN CCMSQTKDQT IAPSSSLFNP 200
    EDFKPLDPTQ EPIFPPELLR LKDTPRKTLR FEGERVTWIQ VSTMEELLDL 250
    KAQHPDAKLV VGNTEIGIEM KFKNMLFPLI ICPAWILELT SVAHGPEGIS 300
    FGAACPLSLV ESVLADAIAT LPEQRTEVFR GVMEQLRWFA GKQVKSVASI 350
    GGNIITASPI SDLNPVLMAS RAKLTLASRG TKRTVWMDHT FFPGYRRTLL 400
    SPEEILVSIV IPYSRKGEFF SAFKQASRRE DDIAKVTSGM RVLFKPGTTE 450
    VQELSLCFGG MADRTVSALK TTPKQLSKSW NEELLQDVCA GLAEELHLAP 500
    DAPGGMVEFR RTLTLSFFFK FYLTVLQKLG RADLEGMCGK LDPTFASATL 550
    LFQKDPPANV QLFQEVPKGQ SEEDMVGRPM PHLAADMQAS GEAVYCDDIP 600
    RYENELSLRL VTSTRAHAKI MSIDTSEAKK VPGFVCFLTS EDVPGSNITG 650
    IFNDETVFAK DEVTCVGHII GAVVADTPEH AHRAARGVKI TYEDLPAIIT 700
    IQDAIKNNSF YGPEVKIEKG DLKKGFSEAD NVVSGELYIG GQEHFYLETH 750
    CTIAVPKGEA GEMELFVSTQ NTMKTQSFIA KMLGVPDNRI VVRVKRMGGG 800
    FGGKETRSTL ISTAVALAAY KTGRPVRCML DRDEDMLITG GRHPFLAKYK 850
    VGFMKTGTIV ALEVAHFSNG GNSEDLSRSI MERAVFHMDN AYKIPNIRGT 900
    GRICKTNLPS NTAFRGFGGP QGMLIAEYWM SEVAVTCGLP AEEVRRKNMY 950
    KEGDLTHFNQ KLEGFTLPRC WDECIASSQY QARKMEVEKF NRENCWKKRG 1000
    LCIIPTKFGI SFTLSFLNQG GALVHVYTDG SVLLTHGGTE MGQGLHTKMV 1050
    QVASRALKIP TSKIHITETS TNTVPNTSPT AASASADLNG QAIYEACQTI 1100
    LKRLEPFKKK NPSGSWESWV MDAYTSAVSL SATGFYKTPN LGYSFETNSG 1150
    NPFHYFSYGV ACSEVEIDCL TGDHKNLRTD IVMDVGSSLN PAIDIGQVEG 1200
    AFVQGLGLFT MEELHYSPEG SLHTRGPSTY KIPAFGSIPI EFRVSLLRDC 1250
    PNKRAIYASK AVGEPPLFLA SSIFFAIKDA IRAARAQHGD SNAKQLFQLD 1300
    SPATPEKIRN ACVDQFTTLC ATGTPENCKS WSVRI 1335
    Length:1,335
    Mass (Da):146,562
    Last modified:July 27, 2011 - v5
    Checksum:i56AD3E0C75E00F98
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti241 – 2411V → I in CAA44705. (PubMed:1590774)Curated
    Sequence conflicti621 – 6211M → T in CAA52997. (PubMed:7835888)Curated
    Sequence conflicti1247 – 12471L → V in CAA52997. (PubMed:7835888)Curated
    Sequence conflicti1247 – 12471L → V in CAA44705. (PubMed:1590774)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75129
    , X75128, X75127, X75126, X75125, X75124, X75123, X75122, X75121, X75120, X75119, X75130, X75131, X75132, X75133, X75134, X75135, X75136, X75137, X75138, X75139, X75140, X75141, X75142, X75143, X75151, X75152, X75153, X75154, X75144, X75145, X75146, X75147, X75148, X75149, X75150 Genomic DNA. Translation: CAA52997.1.
    X62932 mRNA. Translation: CAA44705.1.
    AC159187 Genomic DNA. No translation available.
    CT025731 Genomic DNA. No translation available.
    CCDSiCCDS28967.1.
    PIRiI48374. XOMSDH.
    RefSeqiNP_035853.2. NM_011723.3.
    UniGeneiMm.11223.

    Genome annotation databases

    EnsembliENSMUST00000024866; ENSMUSP00000024866; ENSMUSG00000024066.
    GeneIDi22436.
    KEGGimmu:22436.
    UCSCiuc008dno.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75129
    , X75128 , X75127 , X75126 , X75125 , X75124 , X75123 , X75122 , X75121 , X75120 , X75119 , X75130 , X75131 , X75132 , X75133 , X75134 , X75135 , X75136 , X75137 , X75138 , X75139 , X75140 , X75141 , X75142 , X75143 , X75151 , X75152 , X75153 , X75154 , X75144 , X75145 , X75146 , X75147 , X75148 , X75149 , X75150 Genomic DNA. Translation: CAA52997.1 .
    X62932 mRNA. Translation: CAA44705.1 .
    AC159187 Genomic DNA. No translation available.
    CT025731 Genomic DNA. No translation available.
    CCDSi CCDS28967.1.
    PIRi I48374. XOMSDH.
    RefSeqi NP_035853.2. NM_011723.3.
    UniGenei Mm.11223.

    3D structure databases

    ProteinModelPortali Q00519.
    SMRi Q00519. Positions 7-166, 197-530, 574-1318.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q00519. 2 interactions.
    MINTi MINT-1866314.
    STRINGi 10090.ENSMUSP00000024866.

    PTM databases

    PhosphoSitei Q00519.

    Proteomic databases

    MaxQBi Q00519.
    PaxDbi Q00519.
    PRIDEi Q00519.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024866 ; ENSMUSP00000024866 ; ENSMUSG00000024066 .
    GeneIDi 22436.
    KEGGi mmu:22436.
    UCSCi uc008dno.1. mouse.

    Organism-specific databases

    CTDi 7498.
    MGIi MGI:98973. Xdh.

    Phylogenomic databases

    eggNOGi COG4630.
    GeneTreei ENSGT00390000003772.
    HOGENOMi HOG000191197.
    HOVERGENi HBG004182.
    InParanoidi Q00519.
    KOi K00106.
    OMAi CDETFFA.
    OrthoDBi EOG7QRQSZ.
    TreeFami TF353036.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-14019.
    SABIO-RK Q00519.

    Miscellaneous databases

    ChiTaRSi XDH. mouse.
    NextBioi 302885.
    PROi Q00519.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q00519.
    Bgeei Q00519.
    CleanExi MM_XDH.
    Genevestigatori Q00519.

    Family and domain databases

    Gene3Di 1.10.150.120. 1 hit.
    3.10.20.30. 1 hit.
    3.30.365.10. 6 hits.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    3.90.1170.50. 1 hit.
    InterProi IPR002888. 2Fe-2S-bd.
    IPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR000674. Ald_Oxase/Xan_DH_a/b.
    IPR016208. Ald_Oxase/xanthine_DH.
    IPR008274. AldOxase/xan_DH_Mopterin-bd.
    IPR012675. Beta-grasp_dom.
    IPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    IPR022407. OxRdtase_Mopterin_BS.
    IPR014309. Xanthine_DH_Mopterin-bd_su.
    IPR014307. Xanthine_DH_ssu.
    [Graphical view ]
    Pfami PF01315. Ald_Xan_dh_C. 1 hit.
    PF02738. Ald_Xan_dh_C2. 1 hit.
    PF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    PF00111. Fer2. 1 hit.
    PF01799. Fer2_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000127. Xanthine_DH. 1 hit.
    SMARTi SM01008. Ald_Xan_dh_C. 1 hit.
    SM01092. CO_deh_flav_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47741. SSF47741. 1 hit.
    SSF54292. SSF54292. 1 hit.
    SSF54665. SSF54665. 1 hit.
    SSF55447. SSF55447. 1 hit.
    SSF56003. SSF56003. 1 hit.
    SSF56176. SSF56176. 1 hit.
    TIGRFAMsi TIGR02963. xanthine_xdhA. 1 hit.
    TIGR02965. xanthine_xdhB. 1 hit.
    PROSITEi PS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS51387. FAD_PCMH. 1 hit.
    PS00559. MOLYBDOPTERIN_EUK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Chromosomal mapping, isolation, and characterization of the mouse xanthine dehydrogenase gene."
      Cazzaniga G., Terao M., Lo Schiavo P., Galbiati F., Segalla F., Seldin M.F., Garattini E.
      Genomics 23:390-402(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
      Tissue: Spleen.
    2. "Molecular cloning of a cDNA coding for mouse liver xanthine dehydrogenase. Regulation of its transcript by interferons in vivo."
      Terao M., Cazzaniga G., Ghezzi P., Bianchi M., Falciani F., Perani P., Garattini E.
      Biochem. J. 283:863-870(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Liver.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "Carboxy-terminal cytoplasmic domain of mouse butyrophilin specifically associates with a 150-kDa protein of mammary epithelial cells and milk fat globule membrane."
      Ishii T., Aoki N., Noda A., Adachi T., Nakamura R., Matsuda T.
      Biochim. Biophys. Acta 1245:285-292(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-9, INTERACTION WITH BTN1A1.

    Entry informationi

    Entry nameiXDH_MOUSE
    AccessioniPrimary (citable) accession number: Q00519
    Secondary accession number(s): E9QLM9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 135 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3