Reviewed,
UniProtKB/Swiss-Prot Q00519 (XDH_MOUSE)
Last modified
February 9, 2010.
Version 95.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Xanthine dehydrogenase/oxidase Including the following 2 domains: 1- Recommended name: Xanthine dehydrogenase Short name=XD EC=1.17.1.4 2- Recommended name: Xanthine oxidase Short name=XO EC=1.17.3.2 Alternative name(s): Xanthine oxidoreductase | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 1335 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species By similarity. |
| Catalytic activity | Xanthine + NAD+ + H2O = urate + NADH. Hypoxanthine + NAD+ + H2O = xanthine + NADH. Xanthine + H2O + O2 = urate + H2O2. |
| Cofactor | Binds 2 2Fe-2S clusters By similarity. FAD By similarity. Binds 1 molybdenum ion (molybdopterin) per subunit. |
| Enzyme regulation | Can be converted from the dehydrogenase form (D) to the oxidase form (O) irreversibly by proteolysis or reversibly through the oxidation of sulfhydryl groups By similarity. |
| Subunit structure | Homodimer. Interacts with BTN1A1. Ref.3 |
| Subcellular location | Cytoplasm By similarity. Peroxisome By similarity. Secreted By similarity. |
| Induction | By interferon. |
| Post-translational modification | Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O) By similarity. Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O) By similarity. |
| Sequence similarities | Belongs to the xanthine dehydrogenase family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding PCMH-type domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.3 | ||||||||
| Chain | 2 – 1335 | 1334 | Xanthine dehydrogenase/oxidase | PRO_0000166085 | |||||||
Regions | |||||||||||
| Domain | 7 – 94 | 88 | 2Fe-2S ferredoxin-type | ||||||||
| Domain | 231 – 416 | 186 | FAD-binding PCMH-type | ||||||||
| Nucleotide binding | 259 – 266 | 8 | FAD By similarity | ||||||||
| Nucleotide binding | 349 – 353 | 5 | FAD By similarity | ||||||||
Sites | |||||||||||
| Active site | 1264 | 1 | Proton acceptor By similarity | ||||||||
| Metal binding | 46 | 1 | Iron-sulfur 1 By similarity | ||||||||
| Metal binding | 51 | 1 | Iron-sulfur 1 By similarity | ||||||||
| Metal binding | 54 | 1 | Iron-sulfur 1 By similarity | ||||||||
| Metal binding | 76 | 1 | Iron-sulfur 1 By similarity | ||||||||
| Metal binding | 115 | 1 | Iron-sulfur 2 By similarity | ||||||||
| Metal binding | 118 | 1 | Iron-sulfur 2 By similarity | ||||||||
| Metal binding | 150 | 1 | Iron-sulfur 2 By similarity | ||||||||
| Metal binding | 152 | 1 | Iron-sulfur 2 By similarity | ||||||||
| Metal binding | 770 | 1 | Molybdenum By similarity | ||||||||
| Metal binding | 801 | 1 | Molybdenum; via carbonyl oxygen By similarity | ||||||||
| Metal binding | 915 | 1 | Molybdenum; via amide nitrogen By similarity | ||||||||
| Metal binding | 1082 | 1 | Molybdenum; via amide nitrogen By similarity | ||||||||
| Binding site | 339 | 1 | FAD By similarity | ||||||||
| Binding site | 362 | 1 | FAD By similarity | ||||||||
| Binding site | 406 | 1 | FAD; via amide nitrogen and carbonyl oxygen By similarity | ||||||||
| Binding site | 424 | 1 | FAD By similarity | ||||||||
| Binding site | 805 | 1 | Substrate By similarity | ||||||||
| Binding site | 883 | 1 | Substrate By similarity | ||||||||
| Binding site | 917 | 1 | Substrate By similarity | ||||||||
| Binding site | 1013 | 1 | Substrate; via amide nitrogen By similarity | ||||||||
Amino acid modifications | |||||||||||
| Modified residue | 224 | 1 | Phosphothreonine By similarity | ||||||||
| Glycosylation | 1076 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 538 ↔ 995 | In oxidase form By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 241 | 1 | V → I in CAA44705. Ref.2 | ||||||||
| Sequence conflict | 621 | 1 | T → M in CAA44705. Ref.2 | ||||||||
Sequences
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References
| [1] | "Chromosomal mapping, isolation, and characterization of the mouse xanthine dehydrogenase gene." Cazzaniga G., Terao M., Lo Schiavo P., Galbiati F., Segalla F., Seldin M.F., Garattini E. Genomics 23:390-402(1994) [PubMed: 7835888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv. Tissue: Spleen. |
| [2] | "Molecular cloning of a cDNA coding for mouse liver xanthine dehydrogenase. Regulation of its transcript by interferons in vivo." Terao M., Cazzaniga G., Ghezzi P., Bianchi M., Falciani F., Perani P., Garattini E. Biochem. J. 283:863-870(1992) [PubMed: 1590774] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Liver. |
| [3] | "Carboxy-terminal cytoplasmic domain of mouse butyrophilin specifically associates with a 150-kDa protein of mammary epithelial cells and milk fat globule membrane." Ishii T., Aoki N., Noda A., Adachi T., Nakamura R., Matsuda T. Biochim. Biophys. Acta 1245:285-292(1995) [PubMed: 8541302] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-9, INTERACTION WITH BTN1A1. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X75129 X75150 Genomic DNA. Translation: CAA52997.1. X62932 mRNA. Translation: CAA44705.1. |
| IPI | IPI00352984. |
| PIR | XOMSDH. I48374. |
| UniGene | Mm.11223 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q00519. |
PTM databases | |
| PhosphoSite | Q00519. |
Proteomic databases | |
| PRIDE | Q00519. |
Genome annotation databases | |
| Ensembl | ENSMUST00000024866; ENSMUSP00000024866; ENSMUSG00000024066; Mus musculus. [Genome view] |
Organism-specific databases | |
| MGI | MGI:98973. Xdh. |
Phylogenomic databases | |
| eggNOG | roNOG11539. |
| HOGENOM | HBG386245. |
| HOVERGEN | Q00519. |
| InParanoid | Q00519. |
| PhylomeDB | Q00519. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-14019. |
| BRENDA | 1.17.1.4. 244. 1.17.3.2. 244. |
Gene expression databases | |
| ArrayExpress | Q00519. |
| Bgee | Q00519. |
| CleanEx | MM_XDH. |
| Genevestigator | Q00519. |
| GermOnline | ENSMUSG00000024066. Mus musculus. |
Family and domain databases | |
| InterPro | IPR002888. 2Fe-2S_bd. IPR006058. 2Fe2S_fd_BS. IPR000674. Ald_Oxase/Xan_DH_a/b. IPR016208. Ald_Oxase/xanthine_DH. IPR008274. AldOxase/xan_DH_Mopterin-bd. IPR012675. b-grasp_ferredoxin-like. IPR005107. CO_DH_flav_C. IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR001041. Ferredoxin. IPR002346. Mopterin_DH_FAD-bd. IPR000572. OxRdtase_Mopterin-bd. IPR014309. Xanthine_DH_Mopterin-bd_su. IPR014307. Xanthine_DH_ssu. [Graphical view] |
| Gene3D | G3DSA:3.30.365.10. Ald_xan_DH_mo_bd. 2 hits. G3DSA:3.90.1170.50. Aldxan_DH_hamm. 1 hit. G3DSA:3.30.390.50. CO_DH_flav_C. 1 hit. G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. |
| Pfam | PF01315. Ald_Xan_dh_C. 1 hit. PF02738. Ald_Xan_dh_C2. 1 hit. PF03450. CO_deh_flav_C. 1 hit. PF00941. FAD_binding_5. 1 hit. PF00111. Fer2. 1 hit. PF01799. Fer2_2. 1 hit. [Graphical view] |
| PIRSF | PIRSF000127. Xanthine_DH. 1 hit. |
| TIGRFAMs | TIGR02963. xanthine_xdhA. 1 hit. TIGR02965. xanthine_xdhB. 1 hit. |
| PROSITE | PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51387. FAD_PCMH. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | XDH_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q00519 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


