Q00512 (GSPE_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type II secretion system protein E
Short name=T2SS protein E

Alternative name(s):
General secretion pathway protein E
Type II traffic warden ATPase

Gene names

Name:	xcpR
Ordered Locus Names:	PA3103

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GSP E family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein secretion by the type II secretion system Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell type II protein secretion system complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Type II secretion system protein E		PRO_0000207289
Regions
	Nucleotide binding	263 – 270	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q00512 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 3E88B7A95D0C4916	FASTA	502	55,525
	10 20 30 40 50 60 MMTAPLPDIA APAAPPRRLP FSFAKRQGLL FLCLEEQYWL ACRPQVELAA IAEAQRFAGR 70 80 90 100 110 120 RLPLKALGED AFNQALAASY QHDSSAAMQL AEDLGGSLDL AALADQVPET EDLMEQEDDA 130 140 150 160 170 180 PIIRLINAIL GEAIRENASD IHLETFEKRL VVRFRVDGVL REVLEPKREL AALLVSRIKV 190 200 210 220 230 240 MARLDIAEKR IPQDGRISLR VGGREVDIRV STLPSANGER VVLRLLDKQA GRLNLQHLGM 250 260 270 280 290 300 SERDRKLMDE TVRKPHGILL VTGPTGSGKT TTLYASLTTL NDRTRNILTV EDPIEYHLEG 310 320 330 340 350 360 IGQTQVNAKV DMTFARGLRA ILRQDPDVVM VGEIRDRETA EIAVQASLTG HLVLSTLHTN 370 380 390 400 410 420 SAIGAITRLV DMGIEPFLLS SSMLGVLAQR LVRVLCPACK EPYRADEAEC ALLGVDPAAP 430 440 450 460 470 480 PTLHRARGCG ECHQHGYRGR TGIYELVVFD DHMRSLIHNE SSEQEMTRHA RTSGPSIRDD 490 500 GRRKVLEGVT TVEEVLRVTR EE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Protein secretion in Pseudomonas aeruginosa: characterization of seven xcp genes and processing of secretory apparatus components by prepilin peptidase." Bally M., Filloux A., Akrim M., Ball G., Lazdunski A., Tommassen J. Mol. Microbiol. 6:1121-1131(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62666 Genomic DNA. Translation: CAA44533.1. AE004091 Genomic DNA. Translation: AAG06491.1.
PIR	SKPSXR. S25384.
RefSeq	NP_251793.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q00512.
SMR	Q00512. Positions 117-497.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA3103.
Proteomic databases
PRIDE	Q00512.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877804.
KEGG	pae:PA3103.
PATRIC	19840779. VBIPseAer58763_3255.
Organism-specific databases
PseudoCAP	PA3103.
Phylogenomic databases
eggNOG	COG2804.
HOGENOM	HOG000008427.
KO	K02454.
OMA	RIRIKLA.
ProtClustDB	CLSK867761.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR013369. T2SS_GspE. IPR001482. T2SS_protein-E. [Graphical view]
Pfam	PF00437. T2SE. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR02533. type_II_gspE. 1 hit.
PROSITE	PS00662. T2SP_E. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSPE_PSEAE

Accession

Primary (citable) accession number: Q00512

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program