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Q00511

- URIC_ASPFL

UniProt

Q00511 - URIC_ASPFL

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Protein
Uricase
Gene
uaZ, uox
Organism
Aspergillus flavus
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.

Catalytic activityi

Urate + O2 + H2O = 5-hydroxyisourate + H2O2.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771Charge relay system
Binding sitei177 – 1771Substrate By similarity
Active sitei229 – 2291Charge relay system

GO - Molecular functioni

  1. urate oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. purine nucleobase metabolic process Source: UniProtKB-KW
  2. urate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism

Enzyme and pathway databases

BRENDAi1.7.3.3. 506.
UniPathwayiUPA00394; UER00650.

Names & Taxonomyi

Protein namesi
Recommended name:
Uricase (EC:1.7.3.3)
Alternative name(s):
Urate oxidase
Gene namesi
Name:uaZ
Synonyms:uox
OrganismiAspergillus flavus
Taxonomic identifieri5059 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

  1. peroxisome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 302301Uricase
PRO_0000165995Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ00511.

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 2113
Turni23 – 253
Beta strandi28 – 4215
Helixi44 – 485
Helixi52 – 543
Helixi58 – 7114
Helixi77 – 9115
Beta strandi95 – 10511
Beta strandi108 – 1125
Beta strandi115 – 1228
Beta strandi128 – 1369
Turni137 – 1393
Beta strandi140 – 15819
Beta strandi174 – 1763
Beta strandi179 – 18911
Beta strandi192 – 1943
Helixi195 – 2006
Helixi202 – 22221
Helixi228 – 24215
Beta strandi246 – 25510
Beta strandi258 – 2603
Turni264 – 2685
Helixi273 – 2753
Beta strandi279 – 2813
Beta strandi287 – 2948

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R4SX-ray1.80A2-302[»]
1R4UX-ray1.65A2-302[»]
1R51X-ray1.75A2-302[»]
1R56X-ray2.30A/B/C/D/E/F/G/H2-302[»]
1WRRX-ray1.64A2-302[»]
1WS2X-ray2.70A/B/C/D2-302[»]
1WS3X-ray3.20A/B/C/D2-302[»]
1XT4X-ray2.01A2-302[»]
1XXJX-ray2.80A/B/C/D2-302[»]
1XY3X-ray3.20A/B/C/D/E/F/G/H2-302[»]
2FUBX-ray2.30A2-302[»]
2FXLX-ray1.76A2-302[»]
2IBAX-ray1.50A2-302[»]
2IC0X-ray1.78A2-302[»]
2ICQX-ray1.75A2-302[»]
2PESX-ray1.60A2-296[»]
2ZKAX-ray1.61A2-302[»]
2ZKBX-ray1.61A2-302[»]
3BJPX-ray1.80A2-302[»]
3BK8X-ray1.60A2-302[»]
3CKSX-ray1.70A2-302[»]
3CKUX-ray1.70A2-302[»]
3F2MX-ray1.80A2-302[»]
3GKOX-ray1.60A2-302[»]
3L8WX-ray1.00A2-296[»]
3L9GX-ray1.75A2-296[»]
3LBGX-ray1.50A2-296[»]
3LD4X-ray1.35A2-296[»]
3OBPX-ray1.50A2-302[»]
3P9FX-ray1.70A2-302[»]
3P9OX-ray1.45A2-302[»]
3PJKX-ray1.70A2-302[»]
3PK3X-ray1.65A2-302[»]
3PK4X-ray1.85A2-302[»]
3PK5X-ray1.75A2-302[»]
3PK6X-ray1.80A2-302[»]
3PK8X-ray1.65A2-302[»]
3PKFX-ray1.65A2-302[»]
3PKGX-ray1.60A2-302[»]
3PKHX-ray1.71A2-302[»]
3PKKX-ray1.73A2-302[»]
3PKLX-ray1.75A2-302[»]
3PKSX-ray1.75A2-302[»]
3PKTX-ray1.75A2-302[»]
3PKUX-ray1.75A2-302[»]
3PLEX-ray1.60A2-302[»]
3PLGX-ray1.60A2-302[»]
3PLHX-ray1.80A2-302[»]
3PLIX-ray1.68A2-302[»]
3PLJX-ray1.73A2-302[»]
3PLMX-ray1.62A2-302[»]
4FSKX-ray1.98A2-302[»]
4N3MOther1.90A2-302[»]
4N9MOther2.30A2-302[»]
4N9SX-ray1.06A2-302[»]
4N9VX-ray1.10A2-302[»]
4OP6X-ray1.65A2-302[»]
4OP9X-ray1.58A2-302[»]
ProteinModelPortaliQ00511.
SMRiQ00511. Positions 3-300.

Miscellaneous databases

EvolutionaryTraceiQ00511.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 592Substrate binding By similarity
Regioni228 – 2292Substrate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi300 – 3023Microbody targeting signal Reviewed prediction

Sequence similaritiesi

Belongs to the uricase family.

Phylogenomic databases

eggNOGiCOG3648.
HOGENOMiHOG000250659.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00511-1 [UniParc]FASTAAdd to Basket

« Hide

MSAVKAARYG KDNVRVYKVH KDEKTGVQTV YEMTVCVLLE GEIETSYTKA    50
DNSVIVATDS IKNTIYITAK QNPVTPPELF GSILGTHFIE KYNHIHAAHV 100
NIVCHRWTRM DIDGKPHPHS FIRDSEEKRN VQVDVVEGKG IDIKSSLSGL 150
TVLKSTNSQF WGFLRDEYTT LKETWDRILS TDVDATWQWK NFSGLQEVRS 200
HVPKFDATWA TAREVTLKTF AEDNSASVQA TMYKMAEQIL ARQQLIETVE 250
YSLPNKHYFE IDLSWHKGLQ NTGKNAEVFA PQSDPNGLIK CTVGRSSLKS 300
KL 302
Length:302
Mass (Da):34,241
Last modified:January 23, 2007 - v3
Checksum:iB4FAD4ED4EC121AE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61766 mRNA. Translation: CAA43896.1.
X61765 Genomic DNA. Translation: CAA43895.1.
PIRiA38097.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61766 mRNA. Translation: CAA43896.1 .
X61765 Genomic DNA. Translation: CAA43895.1 .
PIRi A38097.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1R4S X-ray 1.80 A 2-302 [» ]
1R4U X-ray 1.65 A 2-302 [» ]
1R51 X-ray 1.75 A 2-302 [» ]
1R56 X-ray 2.30 A/B/C/D/E/F/G/H 2-302 [» ]
1WRR X-ray 1.64 A 2-302 [» ]
1WS2 X-ray 2.70 A/B/C/D 2-302 [» ]
1WS3 X-ray 3.20 A/B/C/D 2-302 [» ]
1XT4 X-ray 2.01 A 2-302 [» ]
1XXJ X-ray 2.80 A/B/C/D 2-302 [» ]
1XY3 X-ray 3.20 A/B/C/D/E/F/G/H 2-302 [» ]
2FUB X-ray 2.30 A 2-302 [» ]
2FXL X-ray 1.76 A 2-302 [» ]
2IBA X-ray 1.50 A 2-302 [» ]
2IC0 X-ray 1.78 A 2-302 [» ]
2ICQ X-ray 1.75 A 2-302 [» ]
2PES X-ray 1.60 A 2-296 [» ]
2ZKA X-ray 1.61 A 2-302 [» ]
2ZKB X-ray 1.61 A 2-302 [» ]
3BJP X-ray 1.80 A 2-302 [» ]
3BK8 X-ray 1.60 A 2-302 [» ]
3CKS X-ray 1.70 A 2-302 [» ]
3CKU X-ray 1.70 A 2-302 [» ]
3F2M X-ray 1.80 A 2-302 [» ]
3GKO X-ray 1.60 A 2-302 [» ]
3L8W X-ray 1.00 A 2-296 [» ]
3L9G X-ray 1.75 A 2-296 [» ]
3LBG X-ray 1.50 A 2-296 [» ]
3LD4 X-ray 1.35 A 2-296 [» ]
3OBP X-ray 1.50 A 2-302 [» ]
3P9F X-ray 1.70 A 2-302 [» ]
3P9O X-ray 1.45 A 2-302 [» ]
3PJK X-ray 1.70 A 2-302 [» ]
3PK3 X-ray 1.65 A 2-302 [» ]
3PK4 X-ray 1.85 A 2-302 [» ]
3PK5 X-ray 1.75 A 2-302 [» ]
3PK6 X-ray 1.80 A 2-302 [» ]
3PK8 X-ray 1.65 A 2-302 [» ]
3PKF X-ray 1.65 A 2-302 [» ]
3PKG X-ray 1.60 A 2-302 [» ]
3PKH X-ray 1.71 A 2-302 [» ]
3PKK X-ray 1.73 A 2-302 [» ]
3PKL X-ray 1.75 A 2-302 [» ]
3PKS X-ray 1.75 A 2-302 [» ]
3PKT X-ray 1.75 A 2-302 [» ]
3PKU X-ray 1.75 A 2-302 [» ]
3PLE X-ray 1.60 A 2-302 [» ]
3PLG X-ray 1.60 A 2-302 [» ]
3PLH X-ray 1.80 A 2-302 [» ]
3PLI X-ray 1.68 A 2-302 [» ]
3PLJ X-ray 1.73 A 2-302 [» ]
3PLM X-ray 1.62 A 2-302 [» ]
4FSK X-ray 1.98 A 2-302 [» ]
4N3M Other 1.90 A 2-302 [» ]
4N9M Other 2.30 A 2-302 [» ]
4N9S X-ray 1.06 A 2-302 [» ]
4N9V X-ray 1.10 A 2-302 [» ]
4OP6 X-ray 1.65 A 2-302 [» ]
4OP9 X-ray 1.58 A 2-302 [» ]
ProteinModelPortali Q00511.
SMRi Q00511. Positions 3-300.
ModBasei Search...
MobiDBi Search...

Chemistry

DrugBanki DB00049. Rasburicase.

Proteomic databases

PRIDEi Q00511.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG3648.
HOGENOMi HOG000250659.

Enzyme and pathway databases

UniPathwayi UPA00394 ; UER00650 .
BRENDAi 1.7.3.3. 506.

Miscellaneous databases

EvolutionaryTracei Q00511.

Family and domain databases

InterProi IPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view ]
Pfami PF01014. Uricase. 2 hits.
[Graphical view ]
PIRSFi PIRSF000241. Urate_oxidase. 1 hit.
PRINTSi PR00093. URICASE.
TIGRFAMsi TIGR03383. urate_oxi. 1 hit.
PROSITEi PS00366. URICASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression in Escherichia coli of the gene encoding Aspergillus flavus urate oxidase."
    Legoux R., Delpech B., Dumont X., Guillemot J.-C., Ramond P., Shire D., Caput D., Ferrara P., Loison G.
    J. Biol. Chem. 267:8565-8570(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 20047.
  2. "Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05-A resolution."
    Colloc'h N., el Hajji M., Bachet B., L'Hermite G., Schiltz M., Prange T., Castro B., Mornon J.-P.
    Nat. Struct. Biol. 4:947-952(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiURIC_ASPFL
AccessioniPrimary (citable) accession number: Q00511
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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