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Protein

Carboxypeptidase E

Gene

Cpe

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.

Catalytic activityi

Release of C-terminal arginine or lysine residues from polypeptides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi114Zinc; catalyticBy similarity1
Metal bindingi117Zinc; catalyticBy similarity1
Metal bindingi248Zinc; catalyticBy similarity1
Active sitei342Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cardiac left ventricle morphogenesis Source: MGI
  • insulin processing Source: MGI
  • protein localization to membrane Source: MGI
  • Wnt signaling pathway Source: MGI

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.17.10 3474

Protein family/group databases

MEROPSiM14.005

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase E (EC:3.4.17.10)
Short name:
CPE
Alternative name(s):
Carboxypeptidase H
Short name:
CPH
Enkephalin convertase
Prohormone-processing carboxypeptidase
Gene namesi
Name:Cpe
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:101932 Cpe

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane, Secreted

Pathology & Biotechi

Involvement in diseasei

Defects in Cpe are the cause of the fat phenotype. Mice homozygous for the fat mutation develop obesity and hyperglycemia that can be suppressed by treatment with exogenous insulin.1 Publication

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27By similarityAdd BLAST27
PropeptideiPRO_000000438628 – 42Activation peptideBy similarityAdd BLAST15
ChainiPRO_000000438743 – 476Carboxypeptidase EAdd BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi139N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi390N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ00493
PeptideAtlasiQ00493
PRIDEiQ00493

PTM databases

PhosphoSitePlusiQ00493

Expressioni

Gene expression databases

BgeeiENSMUSG00000037852
CleanExiMM_CPE
ExpressionAtlasiQ00493 baseline and differential
GenevisibleiQ00493 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198855, 1 interactor
IntActiQ00493, 3 interactors
MINTiQ00493
STRINGi10090.ENSMUSP00000048555

Structurei

3D structure databases

ProteinModelPortaliQ00493
SMRiQ00493
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2649 Eukaryota
ENOG410XX0H LUCA
GeneTreeiENSGT00760000119124
HOGENOMiHOG000232185
HOVERGENiHBG003410
InParanoidiQ00493
KOiK01294
OMAiEELMDWW
OrthoDBiEOG091G06A9
PhylomeDBiQ00493
TreeFamiTF315592

Family and domain databases

InterProiView protein in InterPro
IPR008969 CarboxyPept-like_regulatory
IPR000834 Peptidase_M14
PfamiView protein in Pfam
PF00246 Peptidase_M14, 1 hit
PRINTSiPR00765 CRBOXYPTASEA
SMARTiView protein in SMART
SM00631 Zn_pept, 1 hit
SUPFAMiSSF49464 SSF49464, 1 hit
PROSITEiView protein in PROSITE
PS00132 CARBOXYPEPT_ZN_1, 1 hit
PS00133 CARBOXYPEPT_ZN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRGGRVLL ALCAALVAGG WLLTAEAQEP GAPAAGMRRR RRLQQEDGIS
60 70 80 90 100
FEYHRYPELR EALVSVWLQC TAISRIYTVG RSFEGRELLV IELSDNPGVH
110 120 130 140 150
EPGEPEFKYI GNMHGNEAVG RELLIFLAQY LCNEYQKGNE TIVNLIHSTR
160 170 180 190 200
IHIMPSLNPD GFEKAASQPG ELKDWFVGRS NAQGIDLNRN FPDLDRIVYV
210 220 230 240 250
NEKEGGPNNH LLKNLKKIVD QNSKLAPETK AVIHWIMDIP FVLSANLHGG
260 270 280 290 300
DLVANYPYDE TRSGTAHEYS SCPDDAIFQS LARAYSSFNP VMSDPNRPPC
310 320 330 340 350
RKNDDDSSFV DGTTNGGAWY SVPGGMQDFN YLSSNCFEIT VELSCEKFPP
360 370 380 390 400
EETLKSYWED NKNSLISYLE QIHRGVKGFV RDLQGNPIAN ATISVDGIDH
410 420 430 440 450
DVTSAKDGDY WRLLAPGNYK LTASAPGYLA ITKKVAVPFS PAVGVDFELE
460 470
SFSERKEEEK EELMEWWKMM SETLNF
Length:476
Mass (Da):53,256
Last modified:July 15, 1998 - v2
Checksum:i82C5318A12A17567
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25A → R in CAA43550 (Ref. 1) Curated1
Sequence conflicti81R → A in CAA43550 (Ref. 1) Curated1
Sequence conflicti167S → W in CAA43550 (Ref. 1) Curated1
Sequence conflicti171E → Q in CAA43550 (Ref. 1) Curated1
Sequence conflicti301R → C in CAA43550 (Ref. 1) Curated1
Sequence conflicti310V → D in CAA43550 (Ref. 1) Curated1
Sequence conflicti334S → SS in CAA43550 (Ref. 1) Curated1
Sequence conflicti344S → T in CAA43550 (Ref. 1) Curated1
Sequence conflicti367S → N in CAA43550 (Ref. 1) Curated1
Sequence conflicti388I → N in CAA43550 (Ref. 1) Curated1
Sequence conflicti425A → D in CAA43550 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti244S → P in hyperproinsulinemia obese fat/fat mice; reduced activity. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61232 mRNA Translation: CAA43550.1
U23184 mRNA Translation: AAB60488.1
BC010197 mRNA Translation: AAH10197.1
CCDSiCCDS22327.1
PIRiS16383
RefSeqiNP_038522.2, NM_013494.4
UniGeneiMm.31395
Mm.470333

Genome annotation databases

EnsembliENSMUST00000048967; ENSMUSP00000048555; ENSMUSG00000037852
GeneIDi12876
KEGGimmu:12876
UCSCiuc009luw.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCBPE_MOUSE
AccessioniPrimary (citable) accession number: Q00493
Secondary accession number(s): Q64439
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 15, 1998
Last modified: May 23, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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