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Q00493

- CBPE_MOUSE

UniProt

Q00493 - CBPE_MOUSE

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Protein

Carboxypeptidase E

Gene

Cpe

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.

Catalytic activityi

Release of C-terminal arginine or lysine residues from polypeptides.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi114 – 1141ZincBy similarity
Metal bindingi117 – 1171ZincBy similarity
Metal bindingi248 – 2481ZincBy similarity
Active sitei342 – 3421NucleophileBy similarity

GO - Molecular functioni

  1. carboxypeptidase activity Source: MGI
  2. metallocarboxypeptidase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. cardiac left ventricle morphogenesis Source: Ensembl
  2. insulin processing Source: MGI
  3. protein localization to membrane Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_207837. Insulin processing.

Protein family/group databases

MEROPSiM14.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase E (EC:3.4.17.10)
Short name:
CPE
Alternative name(s):
Carboxypeptidase H
Short name:
CPH
Enkephalin convertase
Prohormone-processing carboxypeptidase
Gene namesi
Name:Cpe
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:101932. Cpe.

Subcellular locationi

Cytoplasmic vesiclesecretory vesicle membrane By similarity; Peripheral membrane protein By similarity. Secreted By similarity
Note: Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions. This binding is Ca2+-dependent (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. Golgi apparatus Source: Ensembl
  4. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane, Secreted

Pathology & Biotechi

Involvement in diseasei

Defects in Cpe are the cause of the fat phenotype. Mice homozygous for the fat mutation develop obesity and hyperglycemia that can be suppressed by treatment with exogenous insulin.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727By similarityAdd
BLAST
Propeptidei28 – 4215Activation peptideBy similarityPRO_0000004386Add
BLAST
Chaini43 – 476434Carboxypeptidase EPRO_0000004387Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ00493.
PaxDbiQ00493.
PRIDEiQ00493.

PTM databases

PhosphoSiteiQ00493.

Expressioni

Gene expression databases

BgeeiQ00493.
CleanExiMM_CPE.
ExpressionAtlasiQ00493. baseline and differential.
GenevestigatoriQ00493.

Interactioni

Protein-protein interaction databases

BioGridi198855. 1 interaction.
IntActiQ00493. 3 interactions.
MINTiMINT-4090042.

Structurei

3D structure databases

ProteinModelPortaliQ00493.
SMRiQ00493. Positions 49-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG322453.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiQ00493.
KOiK01294.
OMAiTIVNLIH.
OrthoDBiEOG7B8S32.
PhylomeDBiQ00493.
TreeFamiTF315592.

Family and domain databases

Gene3Di2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00493-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGRGGRVLL ALCAALVAGG WLLTAEAQEP GAPAAGMRRR RRLQQEDGIS
60 70 80 90 100
FEYHRYPELR EALVSVWLQC TAISRIYTVG RSFEGRELLV IELSDNPGVH
110 120 130 140 150
EPGEPEFKYI GNMHGNEAVG RELLIFLAQY LCNEYQKGNE TIVNLIHSTR
160 170 180 190 200
IHIMPSLNPD GFEKAASQPG ELKDWFVGRS NAQGIDLNRN FPDLDRIVYV
210 220 230 240 250
NEKEGGPNNH LLKNLKKIVD QNSKLAPETK AVIHWIMDIP FVLSANLHGG
260 270 280 290 300
DLVANYPYDE TRSGTAHEYS SCPDDAIFQS LARAYSSFNP VMSDPNRPPC
310 320 330 340 350
RKNDDDSSFV DGTTNGGAWY SVPGGMQDFN YLSSNCFEIT VELSCEKFPP
360 370 380 390 400
EETLKSYWED NKNSLISYLE QIHRGVKGFV RDLQGNPIAN ATISVDGIDH
410 420 430 440 450
DVTSAKDGDY WRLLAPGNYK LTASAPGYLA ITKKVAVPFS PAVGVDFELE
460 470
SFSERKEEEK EELMEWWKMM SETLNF
Length:476
Mass (Da):53,256
Last modified:July 15, 1998 - v2
Checksum:i82C5318A12A17567
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251A → R in CAA43550. 1 PublicationCurated
Sequence conflicti81 – 811R → A in CAA43550. 1 PublicationCurated
Sequence conflicti167 – 1671S → W in CAA43550. 1 PublicationCurated
Sequence conflicti171 – 1711E → Q in CAA43550. 1 PublicationCurated
Sequence conflicti301 – 3011R → C in CAA43550. 1 PublicationCurated
Sequence conflicti310 – 3101V → D in CAA43550. 1 PublicationCurated
Sequence conflicti334 – 3341S → SS in CAA43550. 1 PublicationCurated
Sequence conflicti344 – 3441S → T in CAA43550. 1 PublicationCurated
Sequence conflicti367 – 3671S → N in CAA43550. 1 PublicationCurated
Sequence conflicti388 – 3881I → N in CAA43550. 1 PublicationCurated
Sequence conflicti425 – 4251A → D in CAA43550. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti244 – 2441S → P in hyperproinsulinemia obese fat/fat mice; reduced activity. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61232 mRNA. Translation: CAA43550.1.
U23184 mRNA. Translation: AAB60488.1.
BC010197 mRNA. Translation: AAH10197.1.
CCDSiCCDS22327.1.
PIRiS16383.
RefSeqiNP_038522.2. NM_013494.3.
UniGeneiMm.31395.
Mm.470333.

Genome annotation databases

EnsembliENSMUST00000048967; ENSMUSP00000048555; ENSMUSG00000037852.
GeneIDi12876.
KEGGimmu:12876.
UCSCiuc009luw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61232 mRNA. Translation: CAA43550.1 .
U23184 mRNA. Translation: AAB60488.1 .
BC010197 mRNA. Translation: AAH10197.1 .
CCDSi CCDS22327.1.
PIRi S16383.
RefSeqi NP_038522.2. NM_013494.3.
UniGenei Mm.31395.
Mm.470333.

3D structure databases

ProteinModelPortali Q00493.
SMRi Q00493. Positions 49-450.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198855. 1 interaction.
IntActi Q00493. 3 interactions.
MINTi MINT-4090042.

Protein family/group databases

MEROPSi M14.005.

PTM databases

PhosphoSitei Q00493.

Proteomic databases

MaxQBi Q00493.
PaxDbi Q00493.
PRIDEi Q00493.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000048967 ; ENSMUSP00000048555 ; ENSMUSG00000037852 .
GeneIDi 12876.
KEGGi mmu:12876.
UCSCi uc009luw.2. mouse.

Organism-specific databases

CTDi 1363.
MGIi MGI:101932. Cpe.

Phylogenomic databases

eggNOGi NOG322453.
HOGENOMi HOG000232185.
HOVERGENi HBG003410.
InParanoidi Q00493.
KOi K01294.
OMAi TIVNLIH.
OrthoDBi EOG7B8S32.
PhylomeDBi Q00493.
TreeFami TF315592.

Enzyme and pathway databases

Reactomei REACT_207837. Insulin processing.

Miscellaneous databases

ChiTaRSi CPE. mouse.
NextBioi 282472.
PROi Q00493.
SOURCEi Search...

Gene expression databases

Bgeei Q00493.
CleanExi MM_CPE.
ExpressionAtlasi Q00493. baseline and differential.
Genevestigatori Q00493.

Family and domain databases

Gene3Di 2.60.40.1120. 1 hit.
InterProi IPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000834. Peptidase_M14.
[Graphical view ]
Pfami PF00246. Peptidase_M14. 1 hit.
[Graphical view ]
PRINTSi PR00765. CRBOXYPTASEA.
SMARTi SM00631. Zn_pept. 1 hit.
[Graphical view ]
SUPFAMi SSF49464. SSF49464. 1 hit.
PROSITEi PS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Parkinson D.
    Submitted (AUG-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Hyperproinsulinaemia in obese fat/fat mice associated with a carboxypeptidase E mutation which reduces enzyme activity."
    Naggert J.K., Fricker L.D., Varlamov O., Nishina P.M., Rouille Y., Steiner D.F., Carroll R.J., Paigen B.J., Leiter E.H.
    Nat. Genet. 10:135-142(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PRO-244.
    Strain: HRS/J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 43-55; 87-108; 363-374 AND 413-420, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiCBPE_MOUSE
AccessioniPrimary (citable) accession number: Q00493
Secondary accession number(s): Q64439
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 15, 1998
Last modified: October 29, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3