Q00473 (RFBM_SALMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannose-1-phosphate guanylyltransferase
EC=2.7.7.13

Alternative name(s):
GDP-mannose pyrophosphorylase
Short name=GMP
Short name=GMPP
GTP--mannose-1-phosphate guanylyltransferase

Gene names

Name:

rfbM

Organism

Salmonella muenchen

Taxonomic identifier

596 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group C2 O antigen.
Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1. Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Mannose-1-phosphate guanylyltransferase		PRO_0000194260

Sequences

	Sequence		Length	Mass (Da)
	Q00473 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: B90AD7D445E2100B	FASTA	473	52,927
	10 20 30 40 50 60 MSKLIPVIMA GGIGSRLWPL SREEHPKQFL SVDGELSMLQ NTIKRLTPLL AGEPLVICND 70 80 90 100 110 120 SHRFLVAEQL RAINKLANNI ILEPVGRNTA PAIALAAFCS LQNVVDEDPL LLVLAADHVI 130 140 150 160 170 180 RDEKVFLKAI NHAEFFATQG KLVTFGIVPT QAETGYGYIC RGEAIGEDAF SVAEFVEKPD 190 200 210 220 230 240 FDTARHYVES EKYYWNSGMF LFRASSYLQE LKDLSPDIYQ ACENAVGSIN PDLDFIRIDK 250 260 270 280 290 300 EAFAMCPSDS IDYAVMEHTR HAVVVPMNAG WSDVGSWSSL WDISKKDPQR NVLHGDIFAY 310 320 330 340 350 360 NSKDNYIYSE KSFISTIGVN NLVIVQTADA LLVSDKDSVQ DVKKVVDYLK ANNRNEHKKH 370 380 390 400 410 420 LEVFRPWGKF SVIHSGDNYL VKRITVKPGA KFAAQMHLHR AEHWIVVSGT ACITKGEEIF 430 440 450 460 470 TISENESTFI PANTVHTLKN PATIPLELIE IQSGTYLAED DIIRLEKHSG YLE

References

[1]

"Molecular analysis of the rfb gene cluster of Salmonella serovar muenchen (strain M67): the genetic basis of the polymorphism between groups C2 and B." Brown P.K., Romana L.K., Reeves P.R. Mol. Microbiol. 6:1385-1394(1992) [PubMed: 1379320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M67.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61917 Genomic DNA. Translation: CAA43915.1.
3D structure databases
ProteinModelPortal	Q00473.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFBM_SALMU

Accession

Primary (citable) accession number: Q00473

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	September 21, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program