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Q00472 (PNPP_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-nitrophenylphosphatase

Short name=PNPPase
EC=3.1.3.41
Gene names
Name:pho2
ORF Names:SPBC15D4.15
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate.

Enzyme regulation

Activity enhanced by Mg2+ ion but inhibited by Zn2+ ion.

Subunit structure

Homodimer.

Post-translational modification

The N-terminus is blocked.

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processprotein dephosphorylation

Inferred from sequence or structural similarity. Source: GeneDB_Spombe

   Cellular componentcytosol

Inferred from direct assay. Source: GeneDB_Spombe

nucleus

Inferred from direct assay. Source: GeneDB_Spombe

   Molecular function4-nitrophenylphosphatase activity

Inferred from mutant phenotype. Source: GeneDB_Spombe

alkaline phosphatase activity

Inferred from sequence or structural similarity. Source: GeneDB_Spombe

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2982984-nitrophenylphosphatase
PRO_0000058480

Experimental info

Sequence conflict1 – 4949MAKKL…LLRSL → MKLLRSLGKSLLFFRVNSFT Ref.1
Sequence conflict981L → V in CAA44597. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q00472 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: AEF1CF3DFD2D7435

FASTA29832,794
        10         20         30         40         50         60 
MAKKLSSPKE YKEFIDKFDV FLFDCDGVLW SGSKPIPGVT DTMKLLRSLG KQIIFVSNNS 

        70         80         90        100        110        120 
TKSRETYMNK INEHGIAAKL EEIYPSAYSS ATYVKKVLKL PADKKVFVLG EAGIEDELDR 

       130        140        150        160        170        180 
VGVAHIGGTD PSLRRALASE DVEKIGPDPS VGAVLCGMDM HVTYLKYCMA FQYLQDPNCA 

       190        200        210        220        230        240 
FLLTNQDSTF PTNGKFLPGS GAISYPLIFS TGRQPKILGK PYDEMMEAII ANVNFDRKKA 

       250        260        270        280        290 
CFVGDRLNTD IQFAKNSNLG GSLLVLTGVS KEEEILEKDA PVVPDYYVES LAKLAETA 

« Hide

References

« Hide 'large scale' references
[1]"Characterisation of the specific p-nitrophenylphosphatase gene and protein of Schizosaccharomyces pombe."
Yang J., Dhamija S.S., Schweingruber M.E.
Eur. J. Biochem. 198:493-497(1991) [PubMed: 1645660] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BD31.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62722 Genomic DNA. Translation: CAA44597.1.
CU329671 Genomic DNA. Translation: CAA20490.1.
PIRS16088.
T39491.
RefSeqNP_596255.1. NM_001022174.1.

3D structure databases

ProteinModelPortalQ00472.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ00472.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC15D4.15.1; SPBC15D4.15.1:pep; SPBC15D4.15.
GeneID2539960.
GenomeReviewsGene locus pho2 in contig CU329671_GR.
KEGGspo:SPBC15D4.15.
NMPDRfig|4896.1.peg.2121.

Organism-specific databases

GeneDB_SpombeSPBC15D4.15.

Phylogenomic databases

eggNOGfuNOG07028.
GeneTreeEFGT00050000003844.
HOGENOMHBG646794.
OMAENEIATS.
OrthoDBEOG4QNR58.

Enzyme and pathway databases

BioCycSPOM-XXX-01:SPOM-XXX-01-004281-MONOMER.

Gene expression databases

ArrayExpressQ00472.

Family and domain databases

InterProIPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
IPR006357. HAD-SF_hydro_IIA.
IPR023215. NPhePase-like_dom.
IPR006349. PGP_euk.
[Graphical view]
Gene3DG3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
G3DSA:3.40.50.10410. NPhePase-like_dom. 1 hit.
KOK01101.
PfamPF00702. Hydrolase. 1 hit.
[Graphical view]
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01460. HAD-SF-IIA. 1 hit.
TIGR01452. PGP_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry namePNPP_SCHPO
AccessionPrimary (citable) accession number: Q00472
Secondary accession number(s): O74320
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 11, 2001
Last modified: December 14, 2011
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names