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Reviewed, UniProtKB/Swiss-Prot Q00472 (PNPP_SCHPO)

Last modified June 16, 2009. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    4-nitrophenylphosphatase
      Short name=PNPPase
    EC=3.1.3.41
Gene names
Name: pho2
ORF Names: SPBC15D4.15
OrganismSchizosaccharomyces pombe (Fission yeast) [Complete proteome]
Taxonomic identifier4896 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate.

Enzyme regulation

Activity enhanced by Mg2+ ion but inhibited by Zn2+ ion.

Subunit structure

Homodimer.

Post-translational modification

The N-terminus is blocked.

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytosol

Inferred from direct assay. Source: GeneDB_SPombe

nucleus

Inferred from direct assay. Source: GeneDB_SPombe

   Molecular function4-nitrophenylphosphatase activity Ref.1

Inferred from direct assay. Source: GeneDB_SPombe

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2982984-nitrophenylphosphatase
PRO_0000058480

Experimental info

Sequence conflict1 – 4949MAKKL…LLRSL → MKLLRSLGKSLLFFRVNSFT Ref.1
Sequence conflict981L → V in CAA44597. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q00472-1 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: AEF1CF3DFD2D7435

FASTA29832,794
        10         20         30         40         50         60 
MAKKLSSPKE YKEFIDKFDV FLFDCDGVLW SGSKPIPGVT DTMKLLRSLG KQIIFVSNNS 

        70         80         90        100        110        120 
TKSRETYMNK INEHGIAAKL EEIYPSAYSS ATYVKKVLKL PADKKVFVLG EAGIEDELDR 

       130        140        150        160        170        180 
VGVAHIGGTD PSLRRALASE DVEKIGPDPS VGAVLCGMDM HVTYLKYCMA FQYLQDPNCA 

       190        200        210        220        230        240 
FLLTNQDSTF PTNGKFLPGS GAISYPLIFS TGRQPKILGK PYDEMMEAII ANVNFDRKKA 

       250        260        270        280        290 
CFVGDRLNTD IQFAKNSNLG GSLLVLTGVS KEEEILEKDA PVVPDYYVES LAKLAETA 

« Hide

References

« Hide 'large scale' references
[1]"Characterisation of the specific p-nitrophenylphosphatase gene and protein of Schizosaccharomyces pombe."
Yang J., Dhamija S.S., Schweingruber M.E.
Eur. J. Biochem. 198:493-497(1991) [PubMed: 1645660] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BD31.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 38366 / 972.

Cross-references

Sequence databases

X62722 Genomic DNA. Translation: CAA44597.1.
CU329671 Genomic DNA. Translation: CAA20490.1.
PIRS16088.
T39491.
RefSeqNP_596255.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID2539960.
KEGGspo:SPBC15D4.15.
NMPDRfig|4896.1.peg.2121.

Organism-specific databases

GeneDB_SpombeSPBC15D4.15.

Phylogenomic databases

OMAQ00472. NSTKSRE.

Enzyme and pathway databases

BioCycSPOM-XXX-01:SPOM-XXX-01-004281-MON.
BRENDA3.1.3.41. 653.

Gene expression databases

ArrayExpressQ00472.

Family and domain databases

InterProIPR005834. Dehalogen-like_hydro.
IPR006357. HAD-SF_hydro_IIA.
IPR006349. PGP_euk.
[Graphical view]
PfamPF00702. Hydrolase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01460. HAD-SF-IIA. 1 hit.
TIGR01452. PGP_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry namePNPP_SCHPO
AccessionPrimary (citable) accession number: Q00472
Secondary accession number(s): O74320
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 11, 2001
Last modified: June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents