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Protein

4-nitrophenylphosphatase

Gene

pho2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein predictedi

Functioni

Catalytic activityi

4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate.

Enzyme regulationi

Activity enhanced by Mg2+ ion but inhibited by Zn2+ ion.

GO - Molecular functioni

  • alkaline phosphatase activity Source: PomBase
  • magnesium ion binding Source: PomBase

GO - Biological processi

  • 4-nitrophenol catabolic process Source: PomBase
  • dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
4-nitrophenylphosphatase (EC:3.1.3.41)
Short name:
PNPPase
Gene namesi
Name:pho2
ORF Names:SPBC15D4.15
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC15D4.15.
PomBaseiSPBC15D4.15. pho2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2982984-nitrophenylphosphatasePRO_0000058480Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiQ00472.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi276504. 48 interactions.
MINTiMINT-4693300.

Structurei

3D structure databases

ProteinModelPortaliQ00472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000068104.
InParanoidiQ00472.
KOiK01101.
OMAiWVATNTD.
OrthoDBiEOG7B05Q3.
PhylomeDBiQ00472.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
3.40.50.10410. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006357. HAD-SF_hydro_IIA.
IPR023215. NPhePase-like_dom.
IPR006349. PGP_euk.
[Graphical view]
PfamiPF13344. Hydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF000915. PGP-type_phosphatase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01460. HAD-SF-IIA. 1 hit.
TIGR01452. PGP_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q00472-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKLSSPKE YKEFIDKFDV FLFDCDGVLW SGSKPIPGVT DTMKLLRSLG
60 70 80 90 100
KQIIFVSNNS TKSRETYMNK INEHGIAAKL EEIYPSAYSS ATYVKKVLKL
110 120 130 140 150
PADKKVFVLG EAGIEDELDR VGVAHIGGTD PSLRRALASE DVEKIGPDPS
160 170 180 190 200
VGAVLCGMDM HVTYLKYCMA FQYLQDPNCA FLLTNQDSTF PTNGKFLPGS
210 220 230 240 250
GAISYPLIFS TGRQPKILGK PYDEMMEAII ANVNFDRKKA CFVGDRLNTD
260 270 280 290
IQFAKNSNLG GSLLVLTGVS KEEEILEKDA PVVPDYYVES LAKLAETA
Length:298
Mass (Da):32,794
Last modified:January 11, 2001 - v2
Checksum:iAEF1CF3DFD2D7435
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 4949MAKKL…LLRSL → MKLLRSLGKSLLFFRVNSFT (PubMed:1645660).CuratedAdd
BLAST
Sequence conflicti98 – 981L → V in CAA44597 (PubMed:1645660).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62722 Genomic DNA. Translation: CAA44597.1.
CU329671 Genomic DNA. Translation: CAA20490.1.
PIRiS16088.
T39491.
RefSeqiNP_596255.1. NM_001022174.2.

Genome annotation databases

EnsemblFungiiSPBC15D4.15.1; SPBC15D4.15.1:pep; SPBC15D4.15.
GeneIDi2539960.
KEGGispo:SPBC15D4.15.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62722 Genomic DNA. Translation: CAA44597.1.
CU329671 Genomic DNA. Translation: CAA20490.1.
PIRiS16088.
T39491.
RefSeqiNP_596255.1. NM_001022174.2.

3D structure databases

ProteinModelPortaliQ00472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276504. 48 interactions.
MINTiMINT-4693300.

Proteomic databases

MaxQBiQ00472.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC15D4.15.1; SPBC15D4.15.1:pep; SPBC15D4.15.
GeneIDi2539960.
KEGGispo:SPBC15D4.15.

Organism-specific databases

EuPathDBiFungiDB:SPBC15D4.15.
PomBaseiSPBC15D4.15. pho2.

Phylogenomic databases

HOGENOMiHOG000068104.
InParanoidiQ00472.
KOiK01101.
OMAiWVATNTD.
OrthoDBiEOG7B05Q3.
PhylomeDBiQ00472.

Miscellaneous databases

PROiQ00472.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
3.40.50.10410. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006357. HAD-SF_hydro_IIA.
IPR023215. NPhePase-like_dom.
IPR006349. PGP_euk.
[Graphical view]
PfamiPF13344. Hydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF000915. PGP-type_phosphatase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01460. HAD-SF-IIA. 1 hit.
TIGR01452. PGP_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterisation of the specific p-nitrophenylphosphatase gene and protein of Schizosaccharomyces pombe."
    Yang J., Dhamija S.S., Schweingruber M.E.
    Eur. J. Biochem. 198:493-497(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BD31.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiPNPP_SCHPO
AccessioniPrimary (citable) accession number: Q00472
Secondary accession number(s): O74320
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 11, 2001
Last modified: June 8, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.