Reviewed, UniProtKB/Swiss-Prot Q00472 (PNPP_SCHPO)

Names and origin

Protein names

Recommended name:
    4-nitrophenylphosphatase
      Short name=PNPPase
    EC=3.1.3.41

Gene names

Name:	pho2
ORF Names:	SPBC15D4.15

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate.
Enzyme regulation	Activity enhanced by Mg2+ ion but inhibited by Zn2+ ion.
Subunit structure	Homodimer.
Post-translational modification	The N-terminus is blocked.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	4-nitrophenylphosphatase activity Ref.1 Inferred from direct assay. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	4-nitrophenylphosphatase		PRO_0000058480
Experimental info
	Sequence conflict	1 – 49	49	MAKKL…LLRSL → MKLLRSLGKSLLFFRVNSFT Ref.1
	Sequence conflict	98	1	L → V in CAA44597. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q00472-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: AEF1CF3DFD2D7435	FASTA	298	32,794
	10 20 30 40 50 60 MAKKLSSPKE YKEFIDKFDV FLFDCDGVLW SGSKPIPGVT DTMKLLRSLG KQIIFVSNNS 70 80 90 100 110 120 TKSRETYMNK INEHGIAAKL EEIYPSAYSS ATYVKKVLKL PADKKVFVLG EAGIEDELDR 130 140 150 160 170 180 VGVAHIGGTD PSLRRALASE DVEKIGPDPS VGAVLCGMDM HVTYLKYCMA FQYLQDPNCA 190 200 210 220 230 240 FLLTNQDSTF PTNGKFLPGS GAISYPLIFS TGRQPKILGK PYDEMMEAII ANVNFDRKKA 250 260 270 280 290 CFVGDRLNTD IQFAKNSNLG GSLLVLTGVS KEEEILEKDA PVVPDYYVES LAKLAETA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterisation of the specific p-nitrophenylphosphatase gene and protein of Schizosaccharomyces pombe." Yang J., Dhamija S.S., Schweingruber M.E. Eur. J. Biochem. 198:493-497(1991) [PubMed: 1645660] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BD31.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X62722 Genomic DNA. Translation: CAA44597.1. CU329671 Genomic DNA. Translation: CAA20490.1.
PIR	S16088. T39491.
RefSeq	NP_596255.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2539960.
KEGG	spo:SPBC15D4.15.
NMPDR	fig|4896.1.peg.2121.
Organism-specific databases
GeneDB_Spombe	SPBC15D4.15.
Phylogenomic databases
OMA	Q00472. NSTKSRE.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004281-MON.
BRENDA	3.1.3.41. 653.
Gene expression databases
ArrayExpress	Q00472.
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR006357. HAD-SF_hydro_IIA. IPR006349. PGP_euk. [Graphical view]
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01460. HAD-SF-IIA. 1 hit. TIGR01452. PGP_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNPP_SCHPO

Accession

Primary (citable) accession number: Q00472
Secondary accession number(s): O74320

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	January 11, 2001
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)