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Protein

Cytochrome b6

Gene

petB

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

Cofactori

heme2 PublicationsNote: Binds 2 heme groups. One heme group is bound covalently by a single cysteine link, the other one non-covalently.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35Heme 1 (covalent; via 1 link)1 Publication1
Metal bindingi86Iron (heme 2 axial ligand); via tele nitrogen1 Publication1
Metal bindingi100Iron (heme 1 axial ligand); via tele nitrogen1 Publication1
Metal bindingi187Iron (heme 2 axial ligand); via tele nitrogen1 Publication1
Metal bindingi202Iron (heme 1 axial ligand); via tele nitrogen1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciCHLAMY:CHRECP008-MONOMER.
MetaCyc:CHRECP008-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6UniRule annotation
Gene namesi
Name:petBUniRule annotation
Encoded oniPlastid; Chloroplast
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas
Proteomesi
  • UP000006906 Componenti: Chloroplast

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei32 – 52HelicalUniRule annotationAdd BLAST21
Transmembranei90 – 110HelicalUniRule annotationAdd BLAST21
Transmembranei116 – 136HelicalUniRule annotationAdd BLAST21
Transmembranei186 – 206HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi204L → P: Leads to defective cytochrome b6-f complex assembly, probably due to lack of heme assembly. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000617871 – 215Cytochrome b6Add BLAST215

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiQ00471.
PRIDEiQ00471.

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.

Protein-protein interaction databases

DIPiDIP-58593N.
STRINGi3055.DAA00911.

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 12Combined sources8
Helixi14 – 23Combined sources10
Helixi33 – 35Combined sources3
Helixi36 – 54Combined sources19
Turni55 – 57Combined sources3
Turni62 – 64Combined sources3
Helixi65 – 74Combined sources10
Helixi79 – 105Combined sources27
Turni106 – 110Combined sources5
Beta strandi111 – 113Combined sources3
Helixi115 – 137Combined sources23
Helixi142 – 153Combined sources12
Helixi154 – 157Combined sources4
Helixi161 – 170Combined sources10
Beta strandi172 – 176Combined sources5
Helixi177 – 188Combined sources12
Helixi190 – 209Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q90X-ray3.10B1-215[»]
ProteinModelPortaliQ00471.
SMRiQ00471.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00471.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b family. PetB subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4663. Eukaryota.
COG1290. LUCA.
InParanoidiQ00471.
KOiK02635.

Family and domain databases

CDDicd00284. Cytochrome_b_N. 1 hit.
Gene3Di1.20.810.10. 1 hit.
HAMAPiMF_00633. Cytb6_f_cytb6. 1 hit.
InterProiIPR005797. Cyt_b/b6_N.
IPR023530. Cyt_B6_PetB.
IPR027387. Cytb/b6-like.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF00033. Cytochrome_B. 1 hit.
[Graphical view]
PIRSFiPIRSF000032. Cytochrome_b6. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
PROSITEiPS51002. CYTB_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVYDWFEE RLEIQAIADD ITSKYVPPHV NIFYCIGGIT FTCFLVQVAT
60 70 80 90 100
GFAMTFYYRP TVAEAFASVQ YIMTDVNFGW LIRSIHRWSA SMMVLMMVLH
110 120 130 140 150
VFRVYLTGGF KRPRELTWVT GVIMAVCTVS FGVTGYSLPW DQVGYWAVKI
160 170 180 190 200
VTGVPDAIPG VGGFIVELLR GGVGVGQATL TRFYSLHTFV LPLLTAVFML
210
MHFLMIRKQG ISGPL
Length:215
Mass (Da):24,165
Last modified:December 1, 1992 - v1
Checksum:iE025FB9ED2ED78B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62905 Genomic DNA. Translation: CAA44690.1.
X72918 Genomic DNA. Translation: CAA51423.1.
FJ423446 Genomic DNA. Translation: ACJ50098.1.
BK000554 Genomic DNA. Translation: DAA00911.1.
PIRiS21253.
RefSeqiNP_958365.1. NC_005353.1.

Genome annotation databases

EnsemblPlantsiDAA00911; DAA00911; DAA00911.
GeneIDi2717017.
GrameneiDAA00911; DAA00911; DAA00911.
KEGGicre:ChreCp008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62905 Genomic DNA. Translation: CAA44690.1.
X72918 Genomic DNA. Translation: CAA51423.1.
FJ423446 Genomic DNA. Translation: ACJ50098.1.
BK000554 Genomic DNA. Translation: DAA00911.1.
PIRiS21253.
RefSeqiNP_958365.1. NC_005353.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q90X-ray3.10B1-215[»]
ProteinModelPortaliQ00471.
SMRiQ00471.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-58593N.
STRINGi3055.DAA00911.

Proteomic databases

PaxDbiQ00471.
PRIDEiQ00471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiDAA00911; DAA00911; DAA00911.
GeneIDi2717017.
GrameneiDAA00911; DAA00911; DAA00911.
KEGGicre:ChreCp008.

Phylogenomic databases

eggNOGiKOG4663. Eukaryota.
COG1290. LUCA.
InParanoidiQ00471.
KOiK02635.

Enzyme and pathway databases

BioCyciCHLAMY:CHRECP008-MONOMER.
MetaCyc:CHRECP008-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ00471.

Family and domain databases

CDDicd00284. Cytochrome_b_N. 1 hit.
Gene3Di1.20.810.10. 1 hit.
HAMAPiMF_00633. Cytb6_f_cytb6. 1 hit.
InterProiIPR005797. Cyt_b/b6_N.
IPR023530. Cyt_B6_PetB.
IPR027387. Cytb/b6-like.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF00033. Cytochrome_B. 1 hit.
[Graphical view]
PIRSFiPIRSF000032. Cytochrome_b6. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
PROSITEiPS51002. CYTB_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB6_CHLRE
AccessioniPrimary (citable) accession number: Q00471
Secondary accession number(s): B7U1F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Creation by mutagenesis of a Pro residue at position 204 (a codon that is known to be RNA edited to a Leu codon in tobacco and maize) leads to assembly defective mutants, indicating that the Leu-204 is essential for proper assembly of the cytochrome b6-f complex. This is probably due to lack of assembly of apocytochrome b6 with one of its heme groups.1 Publication
Heme 1 (or BH or b566) is high-potential and absorbs at about 566 nm, and heme 2 (or BL or b562) is low-potential and absorbs at about 562 nm.UniRule annotation

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.