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Q00471

- CYB6_CHLRE

UniProt

Q00471 - CYB6_CHLRE

Protein

Cytochrome b6

Gene

petB

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

    Cofactori

    Binds 2 heme groups. One heme group is bound covalently by a single cysteine link, the other one non-covalently.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei35 – 351Heme 1 (covalent; via 1 link)
    Metal bindingi86 – 861Iron (heme 2 axial ligand)
    Metal bindingi100 – 1001Iron (heme 1 axial ligand)
    Metal bindingi187 – 1871Iron (heme 2 axial ligand)
    Metal bindingi202 – 2021Iron (heme 1 axial ligand)

    GO - Molecular functioni

    1. electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Source: UniProtKB-HAMAP
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. oxidoreductase activity Source: InterPro

    GO - Biological processi

    1. photosynthesis Source: UniProtKB-HAMAP
    2. respiratory electron transport chain Source: InterPro

    Keywords - Biological processi

    Electron transport, Photosynthesis, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciCHLAMY:CHRECP008-MONOMER.
    MetaCyc:CHRECP008-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b6UniRule annotation
    Gene namesi
    Name:petBUniRule annotation
    Encoded oniPlastid; Chloroplast
    OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
    Taxonomic identifieri3055 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas
    ProteomesiUP000006906: Chloroplast

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast thylakoid membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Thylakoid

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi204 – 2041L → P: Leads to defective cytochrome b6-f complex assembly, probably due to lack of heme assembly. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 215215Cytochrome b6PRO_0000061787Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Proteomic databases

    PRIDEiQ00471.

    Interactioni

    Subunit structurei

    The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.

    Protein-protein interaction databases

    DIPiDIP-58593N.

    Structurei

    Secondary structure

    1
    215
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 128
    Helixi14 – 2310
    Helixi33 – 353
    Helixi36 – 5419
    Turni55 – 573
    Turni62 – 643
    Helixi65 – 7410
    Helixi79 – 10527
    Turni106 – 1105
    Beta strandi111 – 1133
    Helixi115 – 13723
    Helixi142 – 15312
    Helixi154 – 1574
    Helixi161 – 17010
    Beta strandi172 – 1765
    Helixi177 – 18812
    Helixi190 – 20920

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Q90X-ray3.10B1-215[»]
    ProteinModelPortaliQ00471.
    SMRiQ00471. Positions 4-215.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ00471.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei32 – 5221HelicalUniRule annotationAdd
    BLAST
    Transmembranei90 – 11021HelicalUniRule annotationAdd
    BLAST
    Transmembranei116 – 13621HelicalUniRule annotationAdd
    BLAST
    Transmembranei186 – 20621HelicalUniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome b family. PetB subfamily.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK02635.

    Family and domain databases

    Gene3Di1.20.810.10. 1 hit.
    HAMAPiMF_00633. Cytb6_f_cytb6.
    InterProiIPR005797. Cyt_b/b6_N.
    IPR023530. Cyt_B6_PetB.
    IPR027387. Cytb/b6-like.
    IPR016174. Di-haem_cyt_TM.
    [Graphical view]
    PfamiPF13631. Cytochrom_B_N_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000032. Cytochrome_b6. 1 hit.
    SUPFAMiSSF81342. SSF81342. 1 hit.
    PROSITEiPS51002. CYTB_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q00471-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKVYDWFEE RLEIQAIADD ITSKYVPPHV NIFYCIGGIT FTCFLVQVAT    50
    GFAMTFYYRP TVAEAFASVQ YIMTDVNFGW LIRSIHRWSA SMMVLMMVLH 100
    VFRVYLTGGF KRPRELTWVT GVIMAVCTVS FGVTGYSLPW DQVGYWAVKI 150
    VTGVPDAIPG VGGFIVELLR GGVGVGQATL TRFYSLHTFV LPLLTAVFML 200
    MHFLMIRKQG ISGPL 215
    Length:215
    Mass (Da):24,165
    Last modified:December 1, 1992 - v1
    Checksum:iE025FB9ED2ED78B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62905 Genomic DNA. Translation: CAA44690.1.
    X72918 Genomic DNA. Translation: CAA51423.1.
    FJ423446 Genomic DNA. Translation: ACJ50098.1.
    BK000554 Genomic DNA. Translation: DAA00911.1.
    PIRiS21253.
    RefSeqiNP_958365.1. NC_005353.1.

    Genome annotation databases

    EnsemblPlantsiDAA00911; DAA00911; DAA00911.
    GeneIDi2717017.
    KEGGicre:ChreCp008.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62905 Genomic DNA. Translation: CAA44690.1 .
    X72918 Genomic DNA. Translation: CAA51423.1 .
    FJ423446 Genomic DNA. Translation: ACJ50098.1 .
    BK000554 Genomic DNA. Translation: DAA00911.1 .
    PIRi S21253.
    RefSeqi NP_958365.1. NC_005353.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Q90 X-ray 3.10 B 1-215 [» ]
    ProteinModelPortali Q00471.
    SMRi Q00471. Positions 4-215.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-58593N.

    Proteomic databases

    PRIDEi Q00471.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi DAA00911 ; DAA00911 ; DAA00911 .
    GeneIDi 2717017.
    KEGGi cre:ChreCp008.

    Phylogenomic databases

    KOi K02635.

    Enzyme and pathway databases

    BioCyci CHLAMY:CHRECP008-MONOMER.
    MetaCyc:CHRECP008-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q00471.

    Family and domain databases

    Gene3Di 1.20.810.10. 1 hit.
    HAMAPi MF_00633. Cytb6_f_cytb6.
    InterProi IPR005797. Cyt_b/b6_N.
    IPR023530. Cyt_B6_PetB.
    IPR027387. Cytb/b6-like.
    IPR016174. Di-haem_cyt_TM.
    [Graphical view ]
    Pfami PF13631. Cytochrom_B_N_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000032. Cytochrome_b6. 1 hit.
    SUPFAMi SSF81342. SSF81342. 1 hit.
    PROSITEi PS51002. CYTB_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequences of the continuous and separated petA, petB and petD chloroplast genes in Chlamydomonas reinhardtii."
      Bueschlen S., Choquet Y., Kuras R., Wollman F.A.
      FEBS Lett. 284:257-262(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 137c / CC-125.
    2. "Nucleotide sequence of the frxC, petB and trnL genes in the chloroplast genome of Chlamydomonas reinhardtii."
      Huang C., Liu X.-Q.
      Plant Mol. Biol. 18:985-988(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome: addressing the mutational-hazard hypothesis."
      Smith D.R., Lee R.W.
      BMC Evol. Biol. 9:120-120(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CC-503.
    4. "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats."
      Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B.
      Plant Cell 14:2659-2679(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, COMPLETE PLASTID GENOME.
    5. "Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
      Pierre Y., Breyton C., Kramer D., Popot J.-L.
      J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: WT12.
    6. "Mutations of cytochrome b6 in Chlamydomonas reinhardtii disclose the functional significance for a proline to leucine conversion by petB editing in maize and tobacco."
      Zito F., Kuras R., Choquet Y., Koessel H., Wollman F.-A.
      Plant Mol. Biol. 33:79-86(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF LEU-204.
      Strain: 137c / CC-125.
    7. "Biochemical and spectroscopic characterization of the covalent binding of heme to cytochrome b6."
      de Vitry C., Desbois A., Redeker V., Zito F., Wollman F.A.
      Biochemistry 43:3956-3968(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: HEME-BINDING.
    8. "An atypical haem in the cytochrome b(6)f complex."
      Stroebel D., Choquet Y., Popot J.-L., Picot D.
      Nature 426:413-418(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).

    Entry informationi

    Entry nameiCYB6_CHLRE
    AccessioniPrimary (citable) accession number: Q00471
    Secondary accession number(s): B7U1F1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Creation by mutagenesis of a Pro residue at position 204 (a codon that is known to be RNA edited to a Leu codon in tobacco and maize) leads to assembly defective mutants, indicating that the Leu-204 is essential for proper assembly of the cytochrome b6-f complex. This is probably due to lack of assembly of apocytochrome b6 with one of its heme groups.
    Heme 1 (or BH or b566) is high-potential and absorbs at about 566 nm, and heme 2 (or BL or b562) is low-potential and absorbs at about 562 nm.UniRule annotation

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3