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Q00471

- CYB6_CHLRE

UniProt

Q00471 - CYB6_CHLRE

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Protein

Cytochrome b6

Gene

petB

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

Cofactori

Binds 2 heme groups. One heme group is bound covalently by a single cysteine link, the other one non-covalently.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351Heme 1 (covalent; via 1 link)
Metal bindingi86 – 861Iron (heme 2 axial ligand)
Metal bindingi100 – 1001Iron (heme 1 axial ligand)
Metal bindingi187 – 1871Iron (heme 2 axial ligand)
Metal bindingi202 – 2021Iron (heme 1 axial ligand)

GO - Molecular functioni

  1. electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Source: UniProtKB-HAMAP
  2. heme binding Source: EnsemblPlants/Gramene
  3. iron ion binding Source: EnsemblPlants/Gramene
  4. oxidoreductase activity Source: EnsemblPlants/Gramene

GO - Biological processi

  1. photosynthesis Source: UniProtKB-HAMAP
  2. respiratory electron transport chain Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciCHLAMY:CHRECP008-MONOMER.
MetaCyc:CHRECP008-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b6UniRule annotation
Gene namesi
Name:petBUniRule annotation
Encoded oniPlastid; Chloroplast
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas
ProteomesiUP000006906: Chloroplast

Subcellular locationi

GO - Cellular componenti

  1. chloroplast thylakoid membrane Source: UniProtKB-HAMAP
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi204 – 2041L → P: Leads to defective cytochrome b6-f complex assembly, probably due to lack of heme assembly. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Cytochrome b6PRO_0000061787Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PRIDEiQ00471.

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer.

Protein-protein interaction databases

DIPiDIP-58593N.

Structurei

Secondary structure

1
215
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 128
Helixi14 – 2310
Helixi33 – 353
Helixi36 – 5419
Turni55 – 573
Turni62 – 643
Helixi65 – 7410
Helixi79 – 10527
Turni106 – 1105
Beta strandi111 – 1133
Helixi115 – 13723
Helixi142 – 15312
Helixi154 – 1574
Helixi161 – 17010
Beta strandi172 – 1765
Helixi177 – 18812
Helixi190 – 20920

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q90X-ray3.10B1-215[»]
ProteinModelPortaliQ00471.
SMRiQ00471. Positions 4-215.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00471.

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei32 – 5221HelicalUniRule annotationAdd
BLAST
Transmembranei90 – 11021HelicalUniRule annotationAdd
BLAST
Transmembranei116 – 13621HelicalUniRule annotationAdd
BLAST
Transmembranei186 – 20621HelicalUniRule annotationAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b family. PetB subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ00471.
KOiK02635.

Family and domain databases

Gene3Di1.20.810.10. 1 hit.
HAMAPiMF_00633. Cytb6_f_cytb6.
InterProiIPR005797. Cyt_b/b6_N.
IPR023530. Cyt_B6_PetB.
IPR027387. Cytb/b6-like.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF13631. Cytochrom_B_N_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000032. Cytochrome_b6. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
PROSITEiPS51002. CYTB_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q00471-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKVYDWFEE RLEIQAIADD ITSKYVPPHV NIFYCIGGIT FTCFLVQVAT
60 70 80 90 100
GFAMTFYYRP TVAEAFASVQ YIMTDVNFGW LIRSIHRWSA SMMVLMMVLH
110 120 130 140 150
VFRVYLTGGF KRPRELTWVT GVIMAVCTVS FGVTGYSLPW DQVGYWAVKI
160 170 180 190 200
VTGVPDAIPG VGGFIVELLR GGVGVGQATL TRFYSLHTFV LPLLTAVFML
210
MHFLMIRKQG ISGPL
Length:215
Mass (Da):24,165
Last modified:December 1, 1992 - v1
Checksum:iE025FB9ED2ED78B4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62905 Genomic DNA. Translation: CAA44690.1.
X72918 Genomic DNA. Translation: CAA51423.1.
FJ423446 Genomic DNA. Translation: ACJ50098.1.
BK000554 Genomic DNA. Translation: DAA00911.1.
PIRiS21253.
RefSeqiNP_958365.1. NC_005353.1.

Genome annotation databases

EnsemblPlantsiDAA00911; DAA00911; DAA00911.
GeneIDi2717017.
KEGGicre:ChreCp008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62905 Genomic DNA. Translation: CAA44690.1 .
X72918 Genomic DNA. Translation: CAA51423.1 .
FJ423446 Genomic DNA. Translation: ACJ50098.1 .
BK000554 Genomic DNA. Translation: DAA00911.1 .
PIRi S21253.
RefSeqi NP_958365.1. NC_005353.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Q90 X-ray 3.10 B 1-215 [» ]
ProteinModelPortali Q00471.
SMRi Q00471. Positions 4-215.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-58593N.

Proteomic databases

PRIDEi Q00471.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi DAA00911 ; DAA00911 ; DAA00911 .
GeneIDi 2717017.
KEGGi cre:ChreCp008.

Phylogenomic databases

InParanoidi Q00471.
KOi K02635.

Enzyme and pathway databases

BioCyci CHLAMY:CHRECP008-MONOMER.
MetaCyc:CHRECP008-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q00471.

Family and domain databases

Gene3Di 1.20.810.10. 1 hit.
HAMAPi MF_00633. Cytb6_f_cytb6.
InterProi IPR005797. Cyt_b/b6_N.
IPR023530. Cyt_B6_PetB.
IPR027387. Cytb/b6-like.
IPR016174. Di-haem_cyt_TM.
[Graphical view ]
Pfami PF13631. Cytochrom_B_N_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000032. Cytochrome_b6. 1 hit.
SUPFAMi SSF81342. SSF81342. 1 hit.
PROSITEi PS51002. CYTB_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequences of the continuous and separated petA, petB and petD chloroplast genes in Chlamydomonas reinhardtii."
    Bueschlen S., Choquet Y., Kuras R., Wollman F.A.
    FEBS Lett. 284:257-262(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 137c / CC-125.
  2. "Nucleotide sequence of the frxC, petB and trnL genes in the chloroplast genome of Chlamydomonas reinhardtii."
    Huang C., Liu X.-Q.
    Plant Mol. Biol. 18:985-988(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome: addressing the mutational-hazard hypothesis."
    Smith D.R., Lee R.W.
    BMC Evol. Biol. 9:120-120(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CC-503.
  4. "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats."
    Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B.
    Plant Cell 14:2659-2679(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, COMPLETE PLASTID GENOME.
  5. "Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
    Pierre Y., Breyton C., Kramer D., Popot J.-L.
    J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: WT12.
  6. "Mutations of cytochrome b6 in Chlamydomonas reinhardtii disclose the functional significance for a proline to leucine conversion by petB editing in maize and tobacco."
    Zito F., Kuras R., Choquet Y., Koessel H., Wollman F.-A.
    Plant Mol. Biol. 33:79-86(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LEU-204.
    Strain: 137c / CC-125.
  7. "Biochemical and spectroscopic characterization of the covalent binding of heme to cytochrome b6."
    de Vitry C., Desbois A., Redeker V., Zito F., Wollman F.A.
    Biochemistry 43:3956-3968(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: HEME-BINDING.
  8. "An atypical haem in the cytochrome b(6)f complex."
    Stroebel D., Choquet Y., Popot J.-L., Picot D.
    Nature 426:413-418(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).

Entry informationi

Entry nameiCYB6_CHLRE
AccessioniPrimary (citable) accession number: Q00471
Secondary accession number(s): B7U1F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Creation by mutagenesis of a Pro residue at position 204 (a codon that is known to be RNA edited to a Leu codon in tobacco and maize) leads to assembly defective mutants, indicating that the Leu-204 is essential for proper assembly of the cytochrome b6-f complex. This is probably due to lack of assembly of apocytochrome b6 with one of its heme groups.
Heme 1 (or BH or b566) is high-potential and absorbs at about 566 nm, and heme 2 (or BL or b562) is low-potential and absorbs at about 562 nm.UniRule annotation

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3