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Protein

Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein

Gene

chlL

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The L component serves as a unique electron donor to the NB-component of the complex, and binds Mg-ATP.UniRule annotation

Catalytic activityi

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Pathwayi: chlorophyll biosynthesis (light-independent)

This protein is involved in the pathway chlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi14MagnesiumUniRule annotation1
Binding sitei39ATPUniRule annotation1
Metal bindingi95Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi129Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15ATPUniRule annotation6
Nucleotide bindingi180 – 181ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processChlorophyll biosynthesis, Photosynthesis
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:CHRECP009-MONOMER.
UniPathwayiUPA00670.

Names & Taxonomyi

Protein namesi
Recommended name:
Light-independent protochlorophyllide reductase iron-sulfur ATP-binding proteinUniRule annotation (EC:1.3.7.7UniRule annotation)
Short name:
DPOR subunit LUniRule annotation
Short name:
LI-POR subunit LUniRule annotation
Gene namesi
Name:chlLUniRule annotation
Synonyms:frxC, gidB
Encoded oniPlastid; Chloroplast
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas
Proteomesi
  • UP000006906 Componenti: Chloroplast

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001395541 – 293Light-independent protochlorophyllide reductase iron-sulfur ATP-binding proteinAdd BLAST293

Proteomic databases

PaxDbiQ00469.

Interactioni

Subunit structurei

Homodimer. Protochlorophyllide reductase is composed of three subunits; ChlL, ChlN and ChlB.UniRule annotation

Protein-protein interaction databases

STRINGi3055.DAA00912.

Structurei

3D structure databases

ProteinModelPortaliQ00469.
SMRiQ00469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotation

Phylogenomic databases

eggNOGiENOG410JACQ. Eukaryota.
COG1348. LUCA.
InParanoidiQ00469.
KOiK04037.

Family and domain databases

CDDicd02032. Bchl_like. 1 hit.
HAMAPiMF_00355. ChlL_BchL. 1 hit.
InterProiView protein in InterPro
IPR030655. NifH/chlL_CS.
IPR000392. NifH/frxC.
IPR027417. P-loop_NTPase.
IPR005971. Protochlorophyllide_ATP-bd.
PANTHERiPTHR42864. PTHR42864. 1 hit.
PTHR42864:SF1. PTHR42864:SF1. 1 hit.
PfamiView protein in Pfam
PF00142. Fer4_NifH. 1 hit.
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01281. DPOR_bchL. 1 hit.
PROSITEiView protein in PROSITE
PS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q00469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLAVYGKGG IGKSTTSCNI SIALRKRGKK VLQIGCDPKH DSTFTLTGFL
60 70 80 90 100
IPTIIDTLSS KDYHYEDIWP EDVIYGGYGG VDCVEAGGPP AGAGCGGYVV
110 120 130 140 150
GETVKLLKEL NAFFEYDVIL FDVLGDVVCG GFAAPLNYAD YCIIVTDNGF
160 170 180 190 200
DALFAANRIA ASVREKARTH PLRLAGLIGN RTSKRDLIDK YVEACPMPVL
210 220 230 240 250
EVLPLIEEIR ISRVKGKTLF EMSNKNNMTS AHMDGSKGDN STVGVSETPS
260 270 280 290
EDYICNFYLN IADQLLTEPE GVIPRELADK ELFTLLSDFY LKI
Length:293
Mass (Da):31,945
Last modified:December 1, 1992 - v1
Checksum:iF0628502BC007D93
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti25R → A in strain: 2137 and CC-503. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60490 Genomic DNA. Translation: CAA43020.1.
X62905 Genomic DNA. Translation: CAA44691.1.
FJ423446 Genomic DNA. Translation: ACJ50099.1.
BK000554 Genomic DNA. Translation: DAA00912.1.
PIRiJQ1675.
RefSeqiNP_958366.1. NC_005353.1.

Genome annotation databases

EnsemblPlantsiDAA00912; DAA00912; DAA00912.
GeneIDi2717052.
GrameneiDAA00912; DAA00912; DAA00912.
KEGGicre:ChreCp009.

Similar proteinsi

Entry informationi

Entry nameiCHLL_CHLRE
AccessioniPrimary (citable) accession number: Q00469
Secondary accession number(s): B7U1F2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 25, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families