Q00465 (RIPA_LUFCY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ribosome-inactivating protein luffin-alpha EC=3.2.2.22 Alternative name(s): rRNA N-glycosidase
Organism	Luffa cylindrica (Smooth loofah) (Sponge gourd)
Taxonomic identifier	3670 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Sicyoeae › Luffa

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Antiviral defense Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	3D-structure
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Chain

20 – 277

258

Ribosome-inactivating protein luffin-alpha

PRO_0000030785

Sites

Active site

179

1

By similarity

Secondary structure

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277

Helix Strand Turn

Details...

Beta strand

22 – 26

5

Helix

31 – 44

14

Beta strand

47 – 51

5

Beta strand

54 – 57

4

Helix

63 – 66

4

Beta strand

67 – 73

7

Beta strand

79 – 85

7

Turn

86 – 88

3

Beta strand

91 – 96

6

Beta strand

99 – 102

4

Helix

106 – 111

6

Turn

112 – 114

3

Beta strand

119 – 123

5

Helix

130 – 137

8

Helix

141 – 143

3

Helix

148 – 159

12

Turn

163 – 165

3

Helix

166 – 176

11

Helix

178 – 182

5

Helix

184 – 192

9

Beta strand

194 – 196

3

Helix

202 – 221

20

Turn

222 – 226

5

Beta strand

227 – 235

9

Beta strand

241 – 246

6

Helix

250 – 253

4

Sequences

	Sequence		Length	Mass (Da)
	Q00465 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: EA17FC27998C25AC	FASTA	277	30,212
	10 20 30 40 50 60 MKRFTVLILA IFVAASTVEA DVRFSLSGSS STSYSKFIGD LRKALPSNGT VYNITLLLSS 70 80 90 100 110 120 ASGASRYTLM TLSNYDGKAI TVAVDVTNVY IMGYLVNSTS YFFNESDAKL ASQYVFKGST 130 140 150 160 170 180 IVTLPYSGNY EKLQTAAGKI REKIPLGFPA LDSAITTLFH YDSTAAAAAF LVIIQTTAEA 190 200 210 220 230 240 SRFKYIEGQI IERISKNQVP SLATISLENE WSALSKQIQL AQTNNGTFKT PVVITDDKGQ 250 260 270 RVEITNVTSK VVTKNIQLLL NYKQNVAAFD EDVSAKH

References

[1]	"Nucleotide sequence of cDNA encoding alpha-luffin, a ribosome-inactivating protein from Luffa cylindrica." Kataoka J., Habuka N., Miyano M., Masuta C., Koiwai A. Plant Mol. Biol. 18:1199-1202(1992) [PubMed: 1600156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Seed.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X62371 mRNA. Translation: CAA44229.1.

PIR

S22494.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2OQA	X-ray	1.40	A/B	21-261	[»]

ProteinModelPortal

Q00465.

SMR

Q00465. Positions 21-261.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]

Gene3D

G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.

Pfam

PF00161. RIP. 1 hit.
[Graphical view]

PRINTS

PR00396. SHIGARICIN.

SUPFAM

SSF56371. Ribosome_inactivat_prot. 1 hit.

PROSITE

PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RIPA_LUFCY

Accession

Primary (citable) accession number: Q00465

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 31, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program