Q00460 (TMOE_PSEME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Toluene-4-monooxygenase system protein E
EC=1.14.13.-

Gene names

Name:

tmoE

Organism

Pseudomonas mendocina

Taxonomic identifier

300 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydroxylates toluene to form p-cresol.
Cofactor	FAD. Fe2+.
Pathway	Xenobiotic degradation; toluene degradation.
Subunit structure	The multicomponent enzyme toluene-4-monooxygenase is formed by the tmoA, tmoB, tmoC, tmoD, tmoE and tmoF polypeptides.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein Iron
Molecular function	Monooxygenase Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen Inferred from electronic annotation. Source: InterPro transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 327

326

Toluene-4-monooxygenase system protein E

PRO_0000072603

Secondary structure

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327

Helix Strand Turn

Details...

Turn

13 – 17

5

Helix

24 – 29

6

Helix

34 – 36

3

Beta strand

42 – 45

4

Helix

50 – 58

9

Turn

59 – 61

3

Helix

69 – 71

3

Helix

80 – 87

8

Helix

91 – 103

13

Helix

106 – 109

4

Helix

115 – 121

7

Turn

122 – 124

3

Helix

125 – 142

18

Helix

146 – 176

31

Helix

186 – 192

7

Helix

200 – 206

7

Helix

215 – 220

6

Helix

223 – 230

8

Helix

232 – 241

10

Helix

246 – 272

27

Helix

278 – 282

5

Helix

286 – 305

20

Sequences

	Sequence		Length	Mass (Da)
	Q00460 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 65C53BDFF57E040D	FASTA	327	38,386
	10 20 30 40 50 60 MSFESKKPMR TWSHLAEMRK KPSEYDIVSR KLHYSTNNPD SPWELSPDSP MNLWYKQYRN 70 80 90 100 110 120 ASPLKHDNWD AFTDPDQLVY RTYNLMQDGQ ESYVQSLFDQ FNEREHDQMV REGWEHTMAR 130 140 150 160 170 180 CYSPLRYLFH CLQMSSAYVQ QMAPASTISN CCILQTADSL RWLTHTAYRT HELSLTYPDA 190 200 210 220 230 240 GLGEHERELW EKEPGWQGLR ELMEKQLTAF DWGEAFVSLN LVVKPMIVES IFKPLQQQAW 250 260 270 280 290 300 ENNDTLLPLL IDSQLKDAER HSRWSKALVK HALENPDNHA VIEGWIEKWR PLADRAAEAY 310 320 LSMLSSDILH AQYLERSTSL RASILTV

References

[1]	"Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase." Yen K.-M., Karl M.R., Blatt L.M., Simon M.J., Winter R.B., Fausset P.R., Lu H.S., Harcourt A.A., Chen K.K. J. Bacteriol. 173:5315-5327(1991) [PubMed: 1885512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-15. Strain: KR1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M65106 Genomic DNA. Translation: AAA26003.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DHG	X-ray	1.85	B/E	1-327	[»]
3DHH	X-ray	1.94	B	1-327	[»]
3DHI	X-ray	1.68	B	1-327	[»]
3GE3	X-ray	1.52	B	1-327	[»]
3GE8	X-ray	2.19	B/F	1-327	[»]
3I5J	X-ray	1.90	B	1-327	[»]
3I63	X-ray	2.09	B	1-327	[»]

ProteinModelPortal

Q00460.

SMR

Q00460. Positions 7-304.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48647N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR009078. Ferritin/RR-like.
IPR012078. MP_mOase_hydro.
IPR003430. Phenol_Hydrox.
IPR012348. Ribncl_red-rel.
[Graphical view]

Gene3D

G3DSA:1.10.620.20. Ribncl_red_rel. 1 hit.

Pfam

PF02332. Phenol_Hydrox. 1 hit.
[Graphical view]

PIRSF

PIRSF000040. MMOH_comp. 1 hit.

SUPFAM

SSF47240. Ferritin/RR_like. 1 hit.

ProtoNet

Search...

Entry information

Entry name

TMOE_PSEME

Accession

Primary (citable) accession number: Q00460

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	June 28, 2011
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program