Q00458 (TMOC_PSEME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Toluene-4-monooxygenase system ferredoxin subunit

Alternative name(s):
Toluene-4-monooxygenase system protein C

Gene names

Name:

tmoC

Organism

Pseudomonas mendocina

Taxonomic identifier

300 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable electron transfer from NADPH, via FAD and the 2Fe-2S center, to the oxygenase activity site of the enzyme.
Cofactor	Binds 1 2Fe-2S cluster.
Pathway	Xenobiotic degradation; toluene degradation.
Subunit structure	The multicomponent enzyme toluene-4-monooxygenase is formed by the tmoA, tmoB, tmoC, tmoD, tmoE and tmoF polypeptides.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin component family. Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism Electron transport Transport
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 112

111

Toluene-4-monooxygenase system ferredoxin subunit

PRO_0000072601

Regions

Domain

4 – 100

97

Rieske

Sites

Metal binding

45

1

Iron-sulfur (2Fe-2S) Potential

Metal binding

47

1

Iron-sulfur (2Fe-2S); via pros nitrogen Potential

Metal binding

64

1

Iron-sulfur (2Fe-2S) Potential

Metal binding

67

1

Iron-sulfur (2Fe-2S); via pros nitrogen Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

112

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Helix

9 – 11

3

Beta strand

16 – 21

6

Beta strand

27 – 33

7

Turn

34 – 36

3

Beta strand

37 – 44

8

Beta strand

46 – 49

4

Helix

52 – 54

3

Beta strand

55 – 58

4

Beta strand

61 – 63

3

Turn

65 – 67

3

Beta strand

70 – 72

3

Turn

73 – 75

3

Beta strand

77 – 83

7

Beta strand

91 – 94

4

Beta strand

97 – 100

4

Sequences

	Sequence		Length	Mass (Da)
	Q00458 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: B13CD9A7FC832D07	FASTA	112	12,326
	10 20 30 40 50 60 MSFEKICSLD DIWVGEMETF ETSDGTEVLI VNSEEHGVKA YQAMCPHQEI LLSEGSYEGG 70 80 90 100 110 VITCRAHLWT FNDGTGHGIN PDDCCLAEYP VEVKGDDIYV STKGILPNKA HS

References

[1]	"Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase." Yen K.-M., Karl M.R., Blatt L.M., Simon M.J., Winter R.B., Fausset P.R., Lu H.S., Harcourt A.A., Chen K.K. J. Bacteriol. 173:5315-5327(1991) [PubMed: 1885512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: KR1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M65106 Genomic DNA. Translation: AAA26001.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SJG	NMR	-	A	1-112	[»]
1VM9	X-ray	1.48	A	2-111	[»]
2Q3W	X-ray	1.48	A	2-112	[»]

ProteinModelPortal

Q00458.

SMR

Q00458. Positions 1-112.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR017941. Rieske_2Fe-2S.
[Graphical view]

Gene3D

G3DSA:2.102.10.10. Rieske_reg. 1 hit.

Pfam

PF00355. Rieske. 1 hit.
[Graphical view]

SUPFAM

SSF50022. Rieske_dom. 1 hit.

PROSITE

PS51296. RIESKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TMOC_PSEME

Accession

Primary (citable) accession number: Q00458

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 30, 2010
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program