Q00457 (TMOB_PSEME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Toluene-4-monooxygenase system protein B
EC=1.14.13.-

Gene names

Name:

tmoB

Organism

Pseudomonas mendocina

Taxonomic identifier

300 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	84 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydroxylates toluene to form p-cresol.
Cofactor	FAD. Fe2+.
Pathway	Xenobiotic degradation; toluene degradation.
Subunit structure	The multicomponent enzyme toluene-4-monooxygenase is formed by the tmoA, tmoB, tmoC, tmoD, tmoE and tmoF polypeptides.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein Iron
Molecular function	Monooxygenase Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 84

83

Toluene-4-monooxygenase system protein B

PRO_0000072600

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

84

Helix Strand Turn

Details...

Beta strand

3 – 10

8

Beta strand

17 – 22

6

Helix

27 – 36

10

Turn

37 – 41

5

Beta strand

50 – 54

5

Turn

67 – 69

3

Beta strand

77 – 82

6

Sequences

	Sequence		Length	Mass (Da)
	Q00457 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 9EB4FE89F0A7218B	FASTA	84	9,588
	10 20 30 40 50 60 MSAFPVHAAF EKDFLVQLVV VDLNDSMDQV AEKVAYHCVN RRVAPREGVM RVRKHRSTEL 70 80 FPRDMTIAES GLNPTEVIDV VFEE

References

[1]	"Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase." Yen K.-M., Karl M.R., Blatt L.M., Simon M.J., Winter R.B., Fausset P.R., Lu H.S., Harcourt A.A., Chen K.K. J. Bacteriol. 173:5315-5327(1991) [PubMed: 1885512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-28. Strain: KR1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M65106 Genomic DNA. Translation: AAA26000.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DHG	X-ray	1.85	C/F	1-84	[»]
3DHH	X-ray	1.94	C	1-84	[»]
3DHI	X-ray	1.68	C	1-84	[»]
3GE3	X-ray	1.52	C	1-84	[»]
3GE8	X-ray	2.19	C/G	1-84	[»]
3I5J	X-ray	1.90	C	1-84	[»]
3I63	X-ray	2.09	C	1-84	[»]

ProteinModelPortal

Q00457.

SMR

Q00457. Positions 3-83.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48645N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR009355. Toluene_mOase_B.
[Graphical view]

Gene3D

G3DSA:3.10.20.270. Toluene_mOase_B. 1 hit.

Pfam

PF06234. TmoB. 1 hit.
[Graphical view]

SUPFAM

SSF110814. Toluene_mOase_B. 1 hit.

ProtoNet

Search...

Entry information

Entry name

TMOB_PSEME

Accession

Primary (citable) accession number: Q00457

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	June 28, 2011
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program