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Q00456 (TMOA_PSEME) Reviewed, UniProtKB/Swiss-Prot

Last modified June 28, 2011. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Toluene-4-monooxygenase system protein A
EC=1.14.13.-
Gene names
Name:tmoA
OrganismPseudomonas mendocina
Taxonomic identifier300 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydroxylates toluene to form p-cresol.

Cofactor

FAD.

Fe2+.

Pathway

Xenobiotic degradation; toluene degradation.

Subunit structure

The multicomponent enzyme toluene-4-monooxygenase is formed by the tmoA, tmoB, tmoC, tmoD, tmoE and tmoF polypeptides.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandFAD
Flavoprotein
Iron
   Molecular functionMonooxygenase
Oxidoreductase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological processaromatic compound catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionmonooxygenase activity

Inferred from electronic annotation. Source: UniProtKB-KW

transition metal ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 500499Toluene-4-monooxygenase system protein A
PRO_0000072599

Secondary structure

1 ........................................................................................ 500
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q00456 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: D6BD8D541614726D

FASTA50057,982
        10         20         30         40         50         60 
MAMHPRKDWY ELTRATNWTP SYVTEEQLFP ERMSGHMGIP LEKWESYDEP YKTSYPEYVS 

        70         80         90        100        110        120 
IQREKDAGAY SVKAALERAK IYENSDPGWI STLKSHYGAI AVGEYAAVTG EGRMARFSKA 

       130        140        150        160        170        180 
PGNRNMATFG MMDELRHGQL QLFFPHEYCK KDRQFDWAWR AYHSNEWAAI AAKHFFDDII 

       190        200        210        220        230        240 
TGRDAISVAI MLTFSFETGF TNMQFLGLAA DAAEAGDYTF ANLISSIQTD ESRHAQQGGP 

       250        260        270        280        290        300 
ALQLLIENGK REEAQKKVDM AIWRAWRLFA VLTGPVMDYY TPLEDRSQSF KEFMYEWIIG 

       310        320        330        340        350        360 
QFERSLIDLG LDKPWYWDLF LKDIDELHHS YHMGVLDWRT TAWWNPAAGV TPEERDWLEE 

       370        380        390        400        410        420 
KYPGWNKRWG RCWDVITENV LNDRMDLVSP ETLPSVCNMS QIPLVGVPGD DWNIEVFSLE 

       430        440        450        460        470        480 
HNGRLYHFGS EVDRWVFQQD PVQYQNHMNI VDRFLAGQIQ PMTLEGALKY MGFQSIEEMG 

       490        500 
KDAHDFAWAD KCKPAMKKSA

« Hide

References

[1]"Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase."
Yen K.-M., Karl M.R., Blatt L.M., Simon M.J., Winter R.B., Fausset P.R., Lu H.S., Harcourt A.A., Chen K.K.
J. Bacteriol. 173:5315-5327(1991) [PubMed: 1885512] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-14.
Strain: KR1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M65106 Genomic DNA. Translation: AAA25999.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3DHGX-ray1.85A/D1-500[»]
3DHHX-ray1.94A1-500[»]
3DHIX-ray1.68A1-500[»]
3GE3X-ray1.52A1-500[»]
3GE8X-ray2.19A/D1-500[»]
3I5JX-ray1.90A1-500[»]
3I63X-ray2.09A1-500[»]
ProteinModelPortalQ00456.
SMRQ00456. Positions 2-488.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-48644N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR009078. Ferritin/RR-like.
IPR003430. Phenol_Hydrox.
IPR012348. Ribncl_red-rel.
IPR007029. YHS.
[Graphical view]
Gene3DG3DSA:1.10.620.20. Ribncl_red_rel. 1 hit.
PfamPF02332. Phenol_Hydrox. 1 hit.
PF04945. YHS. 1 hit.
[Graphical view]
SUPFAMSSF47240. Ferritin/RR_like. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTMOA_PSEME
AccessionPrimary (citable) accession number: Q00456
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: June 28, 2011
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

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