Reviewed, UniProtKB/Swiss-Prot Q00449 (MDR49_DROME)

Names and origin

Protein names

Recommended name:
    Multidrug resistance protein homolog 49
    EC=3.6.3.44
Alternative name(s):
    P-glycoprotein 49

Gene names

Name:	Mdr49
ORF Names:	CG3879

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	germ cell migration Inferred from mutant phenotype. Source: FlyBase multidrug transport Ref.1 Non-traceable author statement. Source: UniProtKB response to hypoxia Inferred from expression pattern. Source: FlyBase
Cellular component	integral to plasma membrane Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VIS7	1	EBI-121715,EBI-179005
pzg	Q9VP57	1	EBI-121715,EBI-87108

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1302	1302	Multidrug resistance protein homolog 49		PRO_0000093347
Regions
	Topological domain	1 – 46	46	Cytoplasmic Potential
	Transmembrane	47 – 67	21	Potential
	Transmembrane	117 – 145	29	Potential
	Transmembrane	193 – 213	21	Potential
	Transmembrane	221 – 240	20	Potential
	Transmembrane	301 – 322	22	Potential
	Transmembrane	340 – 360	21	Potential
	Topological domain	361 – 734	374	Cytoplasmic Potential
	Transmembrane	735 – 756	22	Potential
	Transmembrane	780 – 801	22	Potential
	Transmembrane	856 – 876	21	Potential
	Transmembrane	878 – 897	20	Potential
	Transmembrane	960 – 980	21	Potential
	Transmembrane	997 – 1017	21	Potential
	Topological domain	1018 – 1302	285	Cytoplasmic Potential
	Domain	46 – 367	322	ABC transmembrane type-1 1
	Domain	402 – 638	237	ABC transporter 1
	Domain	735 – 1023	289	ABC transmembrane type-1 2
	Domain	1059 – 1297	239	ABC transporter 2
	Nucleotide binding	437 – 444	8	ATP 1 Potential
	Nucleotide binding	1094 – 1101	8	ATP 2 Potential
Amino acid modifications
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	697	1	S → N
	Natural variant	712	1	D → N
	Natural variant	952	1	V → I

Sequences

	Sequence		Length	Mass (Da)
	Q00449-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 4720C169455C28CD	FASTA	1,302	142,725
	10 20 30 40 50 60 MVKKEESRLP QAGDFQLKEG SVVDATRKYS YFDLFRYSTR CERFLLVVSL LVATAASAFI 70 80 90 100 110 120 PYFMIIYGEF TSLLVDRTVG VGTSSPAFAL PMFGGGQQLT NASKEENNQA IIDDATAFGI 130 140 150 160 170 180 GSLVGSVAMF LLITLAIDLA NRIALNQIDR IRKLFLEAML RQDIAWYDTS SGSNFASKMT 190 200 210 220 230 240 EDLDKLKEGI GEKIVIVVFL IMTFVIGIVS AFVYGWKLTL VVLSCVPFII AATSVVARLQ 250 260 270 280 290 300 GSLAEKELKS YSDAANVVEE VFSGIRTVFA FSGQEKEKER FGKLLIPAEN TGRKKGLYSG 310 320 330 340 350 360 MGNALSWLII YLCMALAIWY GVTLILDERD LPDRVYTPAV LVIVLFAVIM GAQNLGFASP 370 380 390 400 410 420 HVEAIAVATA AGQTLFNIID RPSQVDPMDE KGNRPENTAG HIRFEGIRFR YPARPDVEIL 430 440 450 460 470 480 KGLTVDVLPG QTVAFVGASG CGKSTLIQLM QRFYDPEAGS VKLDGRDLRT LNVGWLRSQI 490 500 510 520 530 540 GVVGQEPVLF ATTIGENIRY GRPSATQADI EKAARAANCH DFITRLPKGY DTQVGEKGAQ 550 560 570 580 590 600 ISGGQKQRIA IARALVRQPQ VLLLDEATSA LDPTSEKRVQ SALELASQGP TTLVVAHRLS 610 620 630 640 650 660 TITNADKIVF LKDGVVAEQG THEELMERRG LYCELVSITQ RKEATEADEG AVAGRPLQKS 670 680 690 700 710 720 QNLSDEETDD DEEDEEEDEE PELQTSGSSR DSGFRASTRR KRRSQRRKKK KDKEVVSKVS 730 740 750 760 770 780 FTQLMKLNSP EWRFIVVGGI ASVMHGATFP LWGLFFGDFF GILSDGDDDV VRAEVLKISM 790 800 810 820 830 840 IFVGIGLMAG LGNMLQTYMF TTAGVKMTTR LRKRAFGTII GQDIAYFDDE RNSVGALCSR 850 860 870 880 890 900 LASDCSNVQG ATGARVGTML QAVATLVVGM VVGFVFSWQQ TLLTLVTLPL VCLSVYLEGR 910 920 930 940 950 960 FIMKSAQKAK ASIEEASQVA VEAITNIRTV NGLCLERQVL DQYVQQIDRV DVACRRKVRF 970 980 990 1000 1010 1020 RGLVFALGQA APFLAYGISM YYGGILVAEE RMNYEDIIKV AEALIFGSWM LGQALAYAPN 1030 1040 1050 1060 1070 1080 VNDAILSAGR LMDLFKRTST QPNPPQSPYN TVEKSEGDIV YENVGFEYPT RKGTPILQGL 1090 1100 1110 1120 1130 1140 NLTIKKSTTV ALVGPSGSGK STCVQLLLRY YDPVSGSVNL SGVPSTEFPL DTLRSKLGLV 1150 1160 1170 1180 1190 1200 SQEPVLFDRT IAENIAYGNN FRDDVSMQEI IEAAKKSNIH NFISALPQGY DTRLGKTSQL 1210 1220 1230 1240 1250 1260 SGGQKQRIAI ARALVRNPKI LILDEATSAL DLESEKVVQQ ALDEARSGRT CLTIAHRLTT 1270 1280 1290 1300 VRNADLICVL KRGVVVEHGT HDELMALNKI YANLYLMQQV SG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of Drosophila multidrug resistance gene homologs." Wu C.-T., Budding M., Griffin M.S., Croop J.M. Mol. Cell. Biol. 11:3940-3948(1991) [PubMed: 2072901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Head.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M59076 mRNA. Translation: AAA28679.1. AE013599 Genomic DNA. Translation: AAF58437.2. AY058791 mRNA. Translation: AAL14020.1.
PIR	A41249.
RefSeq	NP_523724.2.
UniGene	Dm.2580
3D structure databases
HSSP	HSSP built from PDB template 1MT0 based on UniProtKB P08716.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q00449. 5 interactions.
STRING	Q00449.
Proteomic databases
PRIDE	Q00449.
Genome annotation databases
Ensembl	FBtr0087801; FBpp0086914; FBgn0004512; Drosophila melanogaster. [Genome view]
GeneID	36428.
KEGG	dme:Dmel_CG3879.
Organism-specific databases
CTD	36428.
FlyBase	FBgn0004512. Mdr49.
Phylogenomic databases
HOGENOM	Q00449.
Enzyme and pathway databases
BRENDA	3.6.3.44. 48.
Gene expression databases
ArrayExpress	Q00449.
GermOnline	CG3879. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001140. ABC_TM_transpt. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
ProDom	PD000006. ABC_transporter. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 2 hits. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	798518.

Entry information

Entry name

MDR49_DROME

Accession

Primary (citable) accession number: Q00449
Secondary accession number(s): Q9V6I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	October 13, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project